e coli bl21 Promega Search Results


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  • 93
    Promega e coli bl21
    Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of <t>BL21/DTβ4</t> in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.
    E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 754 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega e coli bl21 de3
    rPFR1 protein production by <t>rec.BL21</t> <t>DE3</t> E.coli cells by SDS-PAGE Lane M: Prestained protein ladder (Puregene, Genetix, PG-PMT 2922) Lane 1: 4h post induction Lane 2: 6h post induction Lane 3: 8h post induction Lanes 4–6: Uninduced control
    E Coli Bl21 De3, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega escherichia coli strain bl21
    rPFR1 protein production by <t>rec.BL21</t> <t>DE3</t> E.coli cells by SDS-PAGE Lane M: Prestained protein ladder (Puregene, Genetix, PG-PMT 2922) Lane 1: 4h post induction Lane 2: 6h post induction Lane 3: 8h post induction Lanes 4–6: Uninduced control
    Escherichia Coli Strain Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega escherichia coli bl21 plyss
    rPFR1 protein production by <t>rec.BL21</t> <t>DE3</t> E.coli cells by SDS-PAGE Lane M: Prestained protein ladder (Puregene, Genetix, PG-PMT 2922) Lane 1: 4h post induction Lane 2: 6h post induction Lane 3: 8h post induction Lanes 4–6: Uninduced control
    Escherichia Coli Bl21 Plyss, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Promega competent escherichia coli bl21
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    Competent Escherichia Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega purification e coli bl21
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    Purification E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega xyr1 e coli bl21
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    Xyr1 E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Promega e coli strain bl21 plyss
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    E Coli Strain Bl21 Plyss, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega e coli bl21 de3 strain
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    E Coli Bl21 De3 Strain, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega eschericha coli bl21
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    Eschericha Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega e coli strain bl21 de3 plyss
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    E Coli Strain Bl21 De3 Plyss, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega e coli strain bl21 codon2
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    E Coli Strain Bl21 Codon2, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega electrocompetent e coli bl21
    Expression of rROP1 in two different bacterial expression hosts, E. coli <t>BL21</t> (DE3) <t>pLysS</t> (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively
    Electrocompetent E Coli Bl21, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.

    Journal: Drug Design, Development and Therapy

    Article Title: A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing

    doi: 10.2147/DDDT.S50183

    Figure Lengend Snippet: Cloning, expression, and large-scale cultivation of dimeric thymosin beta 4 (DTβ4) engineered bacteria. ( A ) Two entire complementary DNA sequences of thymosin beta 4 (Tβ4) were constructed into a prokaryotic expression plasmid with a small DNA linker (GGTTCT). The results show a 267 bp fragment with correct sequence as expected (arrow). ( B ) Five colonies obtained after the transformation of Escherichia coli were randomly picked to test the protein expression on a small scale with sodium dodecyl sulfate polyacrylamide gel electrophoresis. After isopropyl β-D-1-thiogalactopyranoside (IPTG) induction, a new protein (arrow) appeared in each culture pellet and accounted for over 15% of all the bacteria proteins (1: molecular ladder; 2–6: protein expression of picked five clones; 7: protein expression without IPTG induction). ( C ) Bacterial growth curve of BL21/DTβ4 in a 10 L fermenter (the arrow indicates the induction time). ( D ) DTβ4 expression (arrow) during fermentation (1: DTβ4 expression without IPTG induction; 2–6: DTβ4 expression every hour after IPTG induction). Abbreviation: DNA, deoxyribonucleic acid.

    Article Snippet: Expression of DTβ4 and large-scale cultivation of engineered bacteria The pET22b-DTβ4 plasmid was transformed into E. coli BL21 (DE3; Promega) using the calcium chloride method.

    Techniques: Clone Assay, Expressing, Construct, Plasmid Preparation, Sequencing, Transformation Assay, Polyacrylamide Gel Electrophoresis

    rPFR1 protein production by rec.BL21 DE3 E.coli cells by SDS-PAGE Lane M: Prestained protein ladder (Puregene, Genetix, PG-PMT 2922) Lane 1: 4h post induction Lane 2: 6h post induction Lane 3: 8h post induction Lanes 4–6: Uninduced control

    Journal: Iranian Journal of Parasitology

    Article Title: Molecular Cloning, Expression and Characterization of Para Flagellar Rod Protein 1 of Trypanosoma evansi

    doi:

    Figure Lengend Snippet: rPFR1 protein production by rec.BL21 DE3 E.coli cells by SDS-PAGE Lane M: Prestained protein ladder (Puregene, Genetix, PG-PMT 2922) Lane 1: 4h post induction Lane 2: 6h post induction Lane 3: 8h post induction Lanes 4–6: Uninduced control

    Article Snippet: E. coli BL21 (DE3) cells (Promega, USA) were transformed with the recombinant PFR1 plasmid following the standard protocol ( ).

    Techniques: SDS Page

    Expression of rROP1 in two different bacterial expression hosts, E. coli BL21 (DE3) pLysS (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Expression and Purification of Recombinant ROP1 of Toxoplasma gondii in Bacteria

    doi:

    Figure Lengend Snippet: Expression of rROP1 in two different bacterial expression hosts, E. coli BL21 (DE3) pLysS (lanes 1 and 2) and Rosetta (lanes 3 and 4). Induced (lanes 2 and 4) and uninduced bacteria (lanes 1 and 3) were analyzed on SDSPAGE. Densitometry analysis using Image J software showed rROP1band attributed to 1.5% and 0.5% of total protein in induced Rosetta (DE3) and BL21 (DE3) pLysS bacteria, respectively

    Article Snippet: E. coli Top 10F’ (Invitrogen, Carlsbad, CA, USA), E. coli BL21 (DE3) PlysS and Rosetta (DE3) (Promega, Madison, WI, USA) were used for cloning and expression of recombinant antigen, respectively.

    Techniques: Expressing, Software