e coli bl21 Merck Kgaa Search Results


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  • 99
    Millipore escherichia coli bl21
    Escherichia Coli Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5196 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli bl21
    SDS-PAGE (a) and western blot (b) analyses of the recombinant BirA protein expressed via pET24-BirA vector in <t>BL21</t> E. coli cells by addition of IPTG to the final concentration of 1 mM. (a) Lane 1: Protein molecular weight marker (Unstained protein molecular weight marker, Fermentas, Lithuania). Different induced colonies (lane 3–7) showed the same size of BirA (36.6 kDa) expression compared with un-induced sample (lane 2) (b) Western blot analysis of recombinant BirA protein by anti-His antibody specific for C-terminal polyhistidine tag indicated the specific 36.6 kDa protein band. Lane 1: Protein marker (PrestainedProtein Ladder, CinnaGen, Iran) Lane 2: Un-induced bacterial lysate. Lane 3: Recombinant BirA protein (36.6 kDa). Arrows indicate the target protein bands
    E Coli Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA bl21 plys escherichia coli
    SDS-PAGE (a) and western blot (b) analyses of the recombinant BirA protein expressed via pET24-BirA vector in <t>BL21</t> E. coli cells by addition of IPTG to the final concentration of 1 mM. (a) Lane 1: Protein molecular weight marker (Unstained protein molecular weight marker, Fermentas, Lithuania). Different induced colonies (lane 3–7) showed the same size of BirA (36.6 kDa) expression compared with un-induced sample (lane 2) (b) Western blot analysis of recombinant BirA protein by anti-His antibody specific for C-terminal polyhistidine tag indicated the specific 36.6 kDa protein band. Lane 1: Protein marker (PrestainedProtein Ladder, CinnaGen, Iran) Lane 2: Un-induced bacterial lysate. Lane 3: Recombinant BirA protein (36.6 kDa). Arrows indicate the target protein bands
    Bl21 Plys Escherichia Coli, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co e coli bl21
    SDS-PAGE (a) and western blot (b) analyses of the recombinant BirA protein expressed via pET24-BirA vector in <t>BL21</t> E. coli cells by addition of IPTG to the final concentration of 1 mM. (a) Lane 1: Protein molecular weight marker (Unstained protein molecular weight marker, Fermentas, Lithuania). Different induced colonies (lane 3–7) showed the same size of BirA (36.6 kDa) expression compared with un-induced sample (lane 2) (b) Western blot analysis of recombinant BirA protein by anti-His antibody specific for C-terminal polyhistidine tag indicated the specific 36.6 kDa protein band. Lane 1: Protein marker (PrestainedProtein Ladder, CinnaGen, Iran) Lane 2: Un-induced bacterial lysate. Lane 3: Recombinant BirA protein (36.6 kDa). Arrows indicate the target protein bands
    E Coli Bl21, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA escherichia coli
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Escherichia Coli, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli strain bl21
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    E Coli Strain Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli bl21 de3
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    E Coli Bl21 De3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore e coli strain bl21
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    E Coli Strain Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck KGaA purification escherichia coli
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Purification Escherichia Coli, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli line bl21 de3
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    E Coli Line Bl21 De3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA e coli bl21 cells
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    E Coli Bl21 Cells, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA escherichia coli strain bl21 de3 plyse
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Escherichia Coli Strain Bl21 De3 Plyse, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA chemical competent e coli bl21 tuner
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Chemical Competent E Coli Bl21 Tuner, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA escherichia coli strain bl21 de3
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Escherichia Coli Strain Bl21 De3, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA purification e coli strain bl21
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Purification E Coli Strain Bl21, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA bl21 rosetta2 e coli strain
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Bl21 Rosetta2 E Coli Strain, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA bl21 de3 competent e coli cells
    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, <t>Escherichia</t> coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.
    Bl21 De3 Competent E Coli Cells, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SDS-PAGE (a) and western blot (b) analyses of the recombinant BirA protein expressed via pET24-BirA vector in BL21 E. coli cells by addition of IPTG to the final concentration of 1 mM. (a) Lane 1: Protein molecular weight marker (Unstained protein molecular weight marker, Fermentas, Lithuania). Different induced colonies (lane 3–7) showed the same size of BirA (36.6 kDa) expression compared with un-induced sample (lane 2) (b) Western blot analysis of recombinant BirA protein by anti-His antibody specific for C-terminal polyhistidine tag indicated the specific 36.6 kDa protein band. Lane 1: Protein marker (PrestainedProtein Ladder, CinnaGen, Iran) Lane 2: Un-induced bacterial lysate. Lane 3: Recombinant BirA protein (36.6 kDa). Arrows indicate the target protein bands

    Journal: Advanced Biomedical Research

    Article Title: Isolation, cloning, and expression of E. coli BirA gene for biotinylation applications

    doi: 10.4103/2277-9175.161576

    Figure Lengend Snippet: SDS-PAGE (a) and western blot (b) analyses of the recombinant BirA protein expressed via pET24-BirA vector in BL21 E. coli cells by addition of IPTG to the final concentration of 1 mM. (a) Lane 1: Protein molecular weight marker (Unstained protein molecular weight marker, Fermentas, Lithuania). Different induced colonies (lane 3–7) showed the same size of BirA (36.6 kDa) expression compared with un-induced sample (lane 2) (b) Western blot analysis of recombinant BirA protein by anti-His antibody specific for C-terminal polyhistidine tag indicated the specific 36.6 kDa protein band. Lane 1: Protein marker (PrestainedProtein Ladder, CinnaGen, Iran) Lane 2: Un-induced bacterial lysate. Lane 3: Recombinant BirA protein (36.6 kDa). Arrows indicate the target protein bands

    Article Snippet: E. coli BL21 (DE3) and pET-24a(+) plasmid (Merck KGaA, Darmstadt, Germany) were used as expression host and vector, respectively.

    Techniques: SDS Page, Western Blot, Recombinant, Plasmid Preparation, Concentration Assay, Molecular Weight, Marker, Expressing

    Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, Escherichia coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.

    Journal: International Journal of Molecular Medicine

    Article Title: Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophages

    doi: 10.3892/ijmm.2020.4613

    Figure Lengend Snippet: Expression, purification, and RNase activity of r Af RNASET2. (A) Analysis of the prokaryotic recombinant expression vector pET-His- Af RNASET2 (without signal peptides, Glutamic acid 26-Arginine 407) by double enzyme digestion. Lane M indicates the DNA marker. The 1,200 bp band indicates the Af RNASET2 cDNA insert. (B) SDS-PAGE analysis of pET-His-Af RNASET2 expression. Lane M, protein marker; lane 1, E. coli transformed with pET-His-Af RNASET2 prior to IPTG induction; lane 2, E. coli transformed with pET-His- Af RNASET2 following IPTG induction; lane 3, sediment from E. coli transformed with pET-His- Af RNASET2 plasmid following IPTG induction, and ultrasonication. (C) SDS-PAGE of renatured r Af RNASET2 protein following purification using Ni-NTA affinity chromatography. Lane M indicates the protein marker. (D) Analysis of RNase activity of r Af RNASET2 (1 µ g) with yeast RNA (50 µ g) at 37°C at 20, 40, 60, 80 and 100 min). (E) Analysis of RNase activity of r Af RNASET2 with or without RNasin (RNAse inhibitor) pretreatment. RNase enzymolysis products were analyzed using 1% agarose gel electrophoresis. E. coli, Escherichia coli ; r, recombinant; Af, Aspergillus fumigatus ; RNASET2, T2 ribonuclease; IPTG, isopropyl-β-d-thiogalactopyranoside.

    Article Snippet: Expression system components and reagents The following reagents were purchased for recombinant protein expression: Escherichia coli (E. coli ) BL21 (DE3) plysS cells (Merck KGaA), pMD 19-T vectors (Takara Biotechnology Co., Ltd.), pET-His vectors (Wuhan Miaoling Bioscience & Technology Co., Ltd.), and primer STAR HS DNA polymerase (Takara Biotechnology Co., Ltd.).

    Techniques: Expressing, Purification, Activity Assay, Recombinant, Plasmid Preparation, Positron Emission Tomography, Marker, SDS Page, Transformation Assay, Affinity Chromatography, Agarose Gel Electrophoresis