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    Agilent technologies escherichia coli e coli bl21 codonplus
    Escherichia Coli E Coli Bl21 Codonplus, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies e coli bl21
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
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    Agilent technologies virus strains escherichia coli bl21 codonplus de3 ril agilent technologies n a chemicals
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
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    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
    E Coli Bl21 Ril, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies e coli bl21 gold
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
    E Coli Bl21 Gold, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies escherichia coli bl21 de3 gold
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
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    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
    E Coli Bl21 De3 Ril, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies escherichia coli bl21 de3 codonplusril
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
    Escherichia Coli Bl21 De3 Codonplusril, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies e coli strain bl21
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
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    Agilent technologies e coli transformants e coli strain bl21
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
    E Coli Transformants E Coli Strain Bl21, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies escherichia coli bl21 de3 pril
    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.
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    Adseverin D5 is not is expressed during  in vitro  OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression.  B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21  E. coli  induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.

    Journal: PLoS ONE

    Article Title: The Actin Binding Protein Adseverin Regulates Osteoclastogenesis

    doi: 10.1371/journal.pone.0109078

    Figure Lengend Snippet: Adseverin D5 is not is expressed during in vitro OCG. A ) PCR detected only the full-length Ads isoform in Day 0 and Day 6 BMMs and Day 0 and Day 4 RAW macrophage cultures. pSE380-Ads (full-length Ads) and pSE380-Ads-D5 (Ads D5 isoform) plasmids were used as positive controls. Equal volumes of cDNA from each representative samples were used as templates. The intensity of the signal is not a quantitative measure of Ads gene expression. B ) Immunoblot analysis also failed to detect the Ads D5 in BMM and RAW macrophage-derived osteoclasts. Lysates from the transformed BL21 E. coli induced to express exogenous protein were used as positive controls. Only the 85 kDa full-length isoform was detected in 20 µg of TCL from Day 6 BMMs and Day 4 RAW macrophages cultures. Ads was not detected in 20 µg of TCL from unstimulated samples.

    Article Snippet: Two distinct bands at 85 kDa and 80 kDa are readily identifiable in lysates of BL21 E. coli induced to express exogenous Ads or Ads D5, respectively.

    Techniques: In Vitro, Polymerase Chain Reaction, Expressing, Derivative Assay, Transformation Assay