Journal: PLoS ONE
Article Title: Identification of Key Contributory Factors Responsible for Vascular Dysfunction in Idiopathic Recurrent Spontaneous Miscarriage
Figure Lengend Snippet: Multivariate data analyses of factors responsible for vascular dysfunction. (A) Scores scatter plot t1 vs. t2 resulting after applying PCA to endometrial expression of various angiogenic and vasoactive factors of controls (green) and IRSM (blue), (B) scores scatter plot t1 vs t2 resulting after applying PLS-DA to endometrial expression of various angiogenic and vasoactive factors of controls (green) and IRSM (blue), (C) Results from the permutation test for the IRSM group suggest a valid PLS-DA model. The vertical axis is the R2 and Q2 values of each model and the horizontal axis shows the correlation between the permuted class vectors and the original class vector. The original class has the correlation 1.0 with itself, defining the high point on the horizontal axis. All R2 and Q2 values calculated from the permuted data are lower than the original model in the validation plot. Y-axis intercepts: R2= (0.0, 0.0189), Q2= (0.0, −0.123). (D) Loading scatter plot indicates factors IL-1β, TNF-α, IFN-γ, TGF-β1, PGE2 are upregulated in IRSM and factors IL-2, IL-4, IL-6, IL-8, IL-10, VEGF, ADM, eNOS, NO are upregulated in controls.
Article Snippet: ELISA Tissue homogenates containing protein concentration of 30 μg/ml were used for estimating the level of IL-1β, TNF-α, IFN-γ, TGF-β1, IL-10, -2, -6, -8, VEGF, eNOS and ADM. VEGF and PGE2 were assessed using anti-human rabbit polyclonal antibody to VEGF (ab9570) and PGE2 (ab-2318; Abcam, Cambridge, UK), respectively employing quantitative direct enzyme immunoassay technique.
Techniques: Expressing, Plasmid Preparation