dynein ic1 2 cytosolic antibody Search Results


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    • cytosolic  (Santa Cruz Biotechnology)
    93
    Santa Cruz Biotechnology cytosolic
    Tax expression in MT-2 cells. (A) Flow cytometry analysis of MT-2 cell culture, showing about 50% of Tax expressing cells (anti-Tax APC diluted 1:100). (B) Western blot of MT-2 or K562 cell fractionation into <t>cytosolic</t> (lanes 1), intermediate (lanes 2), and nuclear (lanes 3) fractions. The figure shows western blots using antibodies against Tax (Covalab, diluted 1:1,000), GAPDH (cytosolic marker, diluted 1:20,000), calreticulin (CRT) ER marker, diluted 1:5,000), and TFIIB (nuclear marker, diluted 1:1,000).
    Cytosolic, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytosolic/product/Santa Cruz Biotechnology
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytosolic - by Bioz Stars, 2023-03
    93/100 stars
    		  Buy from Supplier
    monoclonal antibodies against cytoplasmic dynein intermediate chain  (Santa Cruz Biotechnology)
    86
    Santa Cruz Biotechnology monoclonal antibodies against cytoplasmic dynein intermediate chain
    (A) An example of Rab11 vesicle movement (yellow circle) along the microtubules (Jupiter-GFP). Scale bar, 1 μm. (B) Acute disruption of microtubules via on-stage injection of colchicine results in immediate inhibition of Rab11 vesicle transport. Images showing the same region before and one minute after injection. Magenta arrowheads indicate residual microtubules after colchicine injection. Yellow dotted circle marks a region where microtubules are completely disrupted. Scale bar, 2 μm. (C) En face view of the constricting domain shortly (< 2 min) after on-stage injection of water or colchicine. Injection of colchicine shortly before but not after the onset of gastrulation (N = 5 embryos for each condition) results in reduced Rab11 vesicle accumulation compared to water injected controls (N = 4 embryos). Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm. (D-F) Quantification of Rab11 vesicle density after water or colchicine injection within a 3.5 μm-stack from apical surface (D) or in a single z plane near apical surface with the highest vesicle density (E). The two approaches are well consistent with each other (F). (G) On-stage injection of <t>dynein</t> antibody rapidly abolishes both apical and basal transport of Rab11 vesicles. Images showing the overlay between the Rab11 channel and the apically or basally targeted vesicle trajectories within a 20-sec time window at the indicated time after injection. N = 5 embryos for both DIC antibody <t>and</t> <t>GST</t> antibody injection. Scale bar, 2 μm. (H) En face view showing inhibition of apical accumulation of Rab11 vesicles upon on-stage injection of dynein antibody immediately before the onset of gastrulation. N = 5 and 6 embryos for GST antibody and dynein antibody injection, respectively. Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm.
    Monoclonal Antibodies Against Cytoplasmic Dynein Intermediate Chain, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/monoclonal antibodies against cytoplasmic dynein intermediate chain/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    monoclonal antibodies against cytoplasmic dynein intermediate chain - by Bioz Stars, 2023-03
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Tax expression in MT-2 cells. (A) Flow cytometry analysis of MT-2 cell culture, showing about 50% of Tax expressing cells (anti-Tax APC diluted 1:100). (B) Western blot of MT-2 or K562 cell fractionation into cytosolic (lanes 1), intermediate (lanes 2), and nuclear (lanes 3) fractions. The figure shows western blots using antibodies against Tax (Covalab, diluted 1:1,000), GAPDH (cytosolic marker, diluted 1:20,000), calreticulin (CRT) ER marker, diluted 1:5,000), and TFIIB (nuclear marker, diluted 1:1,000).

    Journal: AIDS Research and Human Retroviruses

    Article Title: Tax Posttranslational Modifications and Interaction with Calreticulin in MT-2 Cells and Human Peripheral Blood Mononuclear Cells of Human T Cell Lymphotropic Virus Type-I-Associated Myelopathy/Tropical Spastic Paraparesis Patients

    doi: 10.1089/aid.2013.0036

    Figure Lengend Snippet: Tax expression in MT-2 cells. (A) Flow cytometry analysis of MT-2 cell culture, showing about 50% of Tax expressing cells (anti-Tax APC diluted 1:100). (B) Western blot of MT-2 or K562 cell fractionation into cytosolic (lanes 1), intermediate (lanes 2), and nuclear (lanes 3) fractions. The figure shows western blots using antibodies against Tax (Covalab, diluted 1:1,000), GAPDH (cytosolic marker, diluted 1:20,000), calreticulin (CRT) ER marker, diluted 1:5,000), and TFIIB (nuclear marker, diluted 1:1,000).

    Article Snippet: Cat. mab0022, diluted 1:1,000) and HTLV-1 Tax hybridoma 168A51-2 (NIH, AIDS Reagent Program, Germantown, MD; Cat. 1316, used as ascitic fluid, diluted 1:1,000), antibodies against ubiquitin (Upstate, Lake Placid, NY; Cat. 04-263, diluted 1:1,000), SUMO-1 (Upstate; Cat. 04-453, diluted 1:1,000), SUMO-2/3 (Abcam Inc., Cambridge, MA; Cat. Ab3742, diluted 1:1,000), GAPDH (Sigma-Aldrich Inc.; Cat. G8795, diluted 1:20,000), TFIIB (Santa Cruz Biotechnology Inc., Santa Cruz, CA; Cat. sc-225, diluted 1:1,000), Dynein IC1/2, cytosolic (Santa Cruz Biotechnology Inc.; Cat. sc-13524, diluted 1:500), MMP-9 (Millipore Merck, Billerica, MA; Cat. MAB3309, diluted 1:500), and polyclonal antibodies against CRT prepared in Dr. Arturo Ferreira's laboratory (diluted 1:5,000 or 1:800,000 according to the antibody titer).

    Techniques: Expressing, Flow Cytometry, Cell Culture, Western Blot, Cell Fractionation, Marker

    (A) An example of Rab11 vesicle movement (yellow circle) along the microtubules (Jupiter-GFP). Scale bar, 1 μm. (B) Acute disruption of microtubules via on-stage injection of colchicine results in immediate inhibition of Rab11 vesicle transport. Images showing the same region before and one minute after injection. Magenta arrowheads indicate residual microtubules after colchicine injection. Yellow dotted circle marks a region where microtubules are completely disrupted. Scale bar, 2 μm. (C) En face view of the constricting domain shortly (< 2 min) after on-stage injection of water or colchicine. Injection of colchicine shortly before but not after the onset of gastrulation (N = 5 embryos for each condition) results in reduced Rab11 vesicle accumulation compared to water injected controls (N = 4 embryos). Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm. (D-F) Quantification of Rab11 vesicle density after water or colchicine injection within a 3.5 μm-stack from apical surface (D) or in a single z plane near apical surface with the highest vesicle density (E). The two approaches are well consistent with each other (F). (G) On-stage injection of dynein antibody rapidly abolishes both apical and basal transport of Rab11 vesicles. Images showing the overlay between the Rab11 channel and the apically or basally targeted vesicle trajectories within a 20-sec time window at the indicated time after injection. N = 5 embryos for both DIC antibody and GST antibody injection. Scale bar, 2 μm. (H) En face view showing inhibition of apical accumulation of Rab11 vesicles upon on-stage injection of dynein antibody immediately before the onset of gastrulation. N = 5 and 6 embryos for GST antibody and dynein antibody injection, respectively. Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm.

    Journal: bioRxiv

    Article Title: A feedback mechanism mediated by myosin II-dependent apical targeting of Rab11 vesicles reinforces apical constriction

    doi: 10.1101/2021.03.07.434309

    Figure Lengend Snippet: (A) An example of Rab11 vesicle movement (yellow circle) along the microtubules (Jupiter-GFP). Scale bar, 1 μm. (B) Acute disruption of microtubules via on-stage injection of colchicine results in immediate inhibition of Rab11 vesicle transport. Images showing the same region before and one minute after injection. Magenta arrowheads indicate residual microtubules after colchicine injection. Yellow dotted circle marks a region where microtubules are completely disrupted. Scale bar, 2 μm. (C) En face view of the constricting domain shortly (< 2 min) after on-stage injection of water or colchicine. Injection of colchicine shortly before but not after the onset of gastrulation (N = 5 embryos for each condition) results in reduced Rab11 vesicle accumulation compared to water injected controls (N = 4 embryos). Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm. (D-F) Quantification of Rab11 vesicle density after water or colchicine injection within a 3.5 μm-stack from apical surface (D) or in a single z plane near apical surface with the highest vesicle density (E). The two approaches are well consistent with each other (F). (G) On-stage injection of dynein antibody rapidly abolishes both apical and basal transport of Rab11 vesicles. Images showing the overlay between the Rab11 channel and the apically or basally targeted vesicle trajectories within a 20-sec time window at the indicated time after injection. N = 5 embryos for both DIC antibody and GST antibody injection. Scale bar, 2 μm. (H) En face view showing inhibition of apical accumulation of Rab11 vesicles upon on-stage injection of dynein antibody immediately before the onset of gastrulation. N = 5 and 6 embryos for GST antibody and dynein antibody injection, respectively. Scale bar, 10 μm. Zoom-in view of regions marked by red boxes are shown in the bottom. Scale bar, 2 μm.

    Article Snippet: For dynein and GST antibody injection, commercial monoclonal antibodies against cytoplasmic dynein intermediate chain (Cat#sc-13524, Santa Cruz Biotechnology) and monoclonal antibody against GST (Cat#sc-138, Santa Cruz Biotechnology) were first concentrated to 2 mg/mL with Amicon Ultra-0.5 mL centrifugal filters before injection (Cat#UFC501024, Millipore Sigma).

    Techniques: Injection, Inhibition