dynamag-2 magnet Thermo Fisher Search Results


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  • 99
    Thermo Fisher magnet stand
    Soluble OPN is produced in the bone microenvironment and enhances human breast cancer cell migration. (A) Goat anti-mouse OPN primary antibody was incubated for 20 min at RT with DynaBeads ® Protein G prior to incubation with BMCM for 30 min at RT. The beads-antibody-antigen complex was removed with a <t>DynaMag™-2</t> magnet. Concentration of OPN in BMCM, BMCM with beads only (no antibody) and BMCM depleted of OPN (ΔOPN) was assessed by ELISA. Data are presented as mean ± SEM (N = 3). (B) MDA-MB-231 and (C) SUM-159 cells were subjected to transwell migration assays (5 x 10 4 cells/well; 8μm pore size) using basal media (DMEM/F12 + Mito + ), BMCM, BMCM depleted of OPN (ΔOPN) or BMCM ΔOPN rescued with GST-hOPN. Plates were incubated at 37°C, 5% CO 2 for 18 hr, fixed and stained. Five high-powered fields of view (HPF) were captured per transwell and migrated cells were analyzed. Data are presented as mean ± SEM (N = 3; fold-change from negative control basal media). * = significantly different than basal media; ϕ = significantly different than BMCM (P≤0.05).
    Magnet Stand, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher dynamag 50 magnet
    Soluble OPN is produced in the bone microenvironment and enhances human breast cancer cell migration. (A) Goat anti-mouse OPN primary antibody was incubated for 20 min at RT with DynaBeads ® Protein G prior to incubation with BMCM for 30 min at RT. The beads-antibody-antigen complex was removed with a <t>DynaMag™-2</t> magnet. Concentration of OPN in BMCM, BMCM with beads only (no antibody) and BMCM depleted of OPN (ΔOPN) was assessed by ELISA. Data are presented as mean ± SEM (N = 3). (B) MDA-MB-231 and (C) SUM-159 cells were subjected to transwell migration assays (5 x 10 4 cells/well; 8μm pore size) using basal media (DMEM/F12 + Mito + ), BMCM, BMCM depleted of OPN (ΔOPN) or BMCM ΔOPN rescued with GST-hOPN. Plates were incubated at 37°C, 5% CO 2 for 18 hr, fixed and stained. Five high-powered fields of view (HPF) were captured per transwell and migrated cells were analyzed. Data are presented as mean ± SEM (N = 3; fold-change from negative control basal media). * = significantly different than basal media; ϕ = significantly different than BMCM (P≤0.05).
    Dynamag 50 Magnet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynamag 50 magnet/product/Thermo Fisher
    Average 93 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    dynamag 50 magnet - by Bioz Stars, 2020-08
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    93
    Thermo Fisher dynamag 2 magnet invitrogen
    Soluble OPN is produced in the bone microenvironment and enhances human breast cancer cell migration. (A) Goat anti-mouse OPN primary antibody was incubated for 20 min at RT with DynaBeads ® Protein G prior to incubation with BMCM for 30 min at RT. The beads-antibody-antigen complex was removed with a <t>DynaMag™-2</t> magnet. Concentration of OPN in BMCM, BMCM with beads only (no antibody) and BMCM depleted of OPN (ΔOPN) was assessed by ELISA. Data are presented as mean ± SEM (N = 3). (B) MDA-MB-231 and (C) SUM-159 cells were subjected to transwell migration assays (5 x 10 4 cells/well; 8μm pore size) using basal media (DMEM/F12 + Mito + ), BMCM, BMCM depleted of OPN (ΔOPN) or BMCM ΔOPN rescued with GST-hOPN. Plates were incubated at 37°C, 5% CO 2 for 18 hr, fixed and stained. Five high-powered fields of view (HPF) were captured per transwell and migrated cells were analyzed. Data are presented as mean ± SEM (N = 3; fold-change from negative control basal media). * = significantly different than basal media; ϕ = significantly different than BMCM (P≤0.05).
    Dynamag 2 Magnet Invitrogen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dynamag 2 magnet invitrogen - by Bioz Stars, 2020-08
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    Image Search Results


    Soluble OPN is produced in the bone microenvironment and enhances human breast cancer cell migration. (A) Goat anti-mouse OPN primary antibody was incubated for 20 min at RT with DynaBeads ® Protein G prior to incubation with BMCM for 30 min at RT. The beads-antibody-antigen complex was removed with a DynaMag™-2 magnet. Concentration of OPN in BMCM, BMCM with beads only (no antibody) and BMCM depleted of OPN (ΔOPN) was assessed by ELISA. Data are presented as mean ± SEM (N = 3). (B) MDA-MB-231 and (C) SUM-159 cells were subjected to transwell migration assays (5 x 10 4 cells/well; 8μm pore size) using basal media (DMEM/F12 + Mito + ), BMCM, BMCM depleted of OPN (ΔOPN) or BMCM ΔOPN rescued with GST-hOPN. Plates were incubated at 37°C, 5% CO 2 for 18 hr, fixed and stained. Five high-powered fields of view (HPF) were captured per transwell and migrated cells were analyzed. Data are presented as mean ± SEM (N = 3; fold-change from negative control basal media). * = significantly different than basal media; ϕ = significantly different than BMCM (P≤0.05).

    Journal: PLoS ONE

    Article Title: Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells

    doi: 10.1371/journal.pone.0177640

    Figure Lengend Snippet: Soluble OPN is produced in the bone microenvironment and enhances human breast cancer cell migration. (A) Goat anti-mouse OPN primary antibody was incubated for 20 min at RT with DynaBeads ® Protein G prior to incubation with BMCM for 30 min at RT. The beads-antibody-antigen complex was removed with a DynaMag™-2 magnet. Concentration of OPN in BMCM, BMCM with beads only (no antibody) and BMCM depleted of OPN (ΔOPN) was assessed by ELISA. Data are presented as mean ± SEM (N = 3). (B) MDA-MB-231 and (C) SUM-159 cells were subjected to transwell migration assays (5 x 10 4 cells/well; 8μm pore size) using basal media (DMEM/F12 + Mito + ), BMCM, BMCM depleted of OPN (ΔOPN) or BMCM ΔOPN rescued with GST-hOPN. Plates were incubated at 37°C, 5% CO 2 for 18 hr, fixed and stained. Five high-powered fields of view (HPF) were captured per transwell and migrated cells were analyzed. Data are presented as mean ± SEM (N = 3; fold-change from negative control basal media). * = significantly different than basal media; ϕ = significantly different than BMCM (P≤0.05).

    Article Snippet: Resulting bead-antibody-antigen complexes were removed from BMCM using a DynaMag-2 magnet (Novex/Life Technologies).

    Techniques: Produced, Migration, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Multiple Displacement Amplification, Staining, Negative Control