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  • 94
    Thermo Fisher cd4 dynabeads
    The functional response to PD-1/CTLA-4 blockade associate directly with CTLA-4 expression but not FoxP3 + Tregs. (A) Correlation between HCV specific effector cytokine response to combined PD-1/CTLA-4 blockade and ex vivo %CTLA-4 + /CD8 but not %PD-1 + /CD8 and %FoxP3 + <t>/CD4.</t> The y-axis represents the sum of CD8 T cells with HCV-specific IFN-γ + TNF-α + , IFN-γ + TNF-α − and IFN-γ − TNF-α + expression during combined PD-1/CTLA-4 blockade from 14 HLA-A2 − patients (6 intrahepatic and 8 peripheral blood responses). R and p-values by the Spearman rank correlation test. (B) Positive correlation between fold expansion of HCV-specific tetramer + CD8 T cells with combined PD-1/CTLA-4 blockade (relative to PD-1 blockade alone) and ex vivo %CTLA-4 + in HCV-specific tetramer+ CD8 T cells in 7 HLA A2+ HCV-infected patients. R- and p-values by the Spearman rank correlation test. (C) (Left) : Liver infiltrating lymphocytes from chronic patient C07 are examined for CD4, CD8 and FoxP3 + T cell subsets before (upper) and after (lower) depletion of CD4 T cells by CD4 <t>Dynabeads</t> (Dynal Inc), resulting in > 99% depletion of CD4 T cells including FoxP3 + CD4 T cells. (Right) : Undepleted and CD4-depleted liver infiltrating lymphocytes were cultured for 7 days with overlapping HCV NS3-derived 15mer peptides (pNS3) in the presence of isotype or blocking antibodies before direct staining for T cell subsets (CD4, FoxP3) and following additional 6 hours of stimulation with media alone (negative control) or pNS3 peptides to examine HCV-specific intracellular IFN-γ and TNF-α expression in CD8 T cells. Combined PD-1/CTLA-4 blockade promoted markedly enhanced HCV-specific cytokine response in undepleted and CD4-depleted cultures regardless of FoxP3 + Tregs.
    Cd4 Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4 dynabeads/product/Thermo Fisher
    Average 94 stars, based on 202 article reviews
    Price from $9.99 to $1999.99
    cd4 dynabeads - by Bioz Stars, 2020-05
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    98
    Thermo Fisher dynabeads
    Optimization of TRAP immunoprecipitation. The amount of antibody required for the TRAP IP was determined by titration. 500 μl hippocampal lysate was incubated with varying amounts of anti-HA antibody from 2.5 to 12.5 μg, followed by pull down of the antibody-ribosome-mRNA complex with protein A <t>dynabeads.</t> After washing, half the beads were used for protein extraction while the other half were used RNA isolation. ( A ) Protein concentration in the input lysate and remaining in the supernatant after IP (Post-IP Lysate) as well as the amount of protein eluted from the beads was determined by Western blotting. Western blots for the ribosomal protein RPP0 and the HA-tagged RPL22 ribosomal protein are shown (i) along with the quantification of the protein bands (ii). ( B ) Concentration of RNA eluted from the beads is shown for each IP. Based on these combined results 10 μg antibody per 500 μl lysate was determined to be the optimal concentration to ensure maximal depletion of the HA-tagged polysomes and give the best signal-to-noise.
    Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 13503 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads/product/Thermo Fisher
    Average 98 stars, based on 13503 article reviews
    Price from $9.99 to $1999.99
    dynabeads - by Bioz Stars, 2020-05
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    95
    Thermo Fisher dynabeads m450
    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and <t>CD3</t> on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 <t>Dynabeads,</t> generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).
    Dynabeads M450, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads m450/product/Thermo Fisher
    Average 95 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    dynabeads m450 - by Bioz Stars, 2020-05
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    99
    Thermo Fisher igg dynabeads dynabeads
    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and <t>CD3</t> on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 <t>Dynabeads,</t> generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).
    Igg Dynabeads Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 3 article reviews
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    95
    Thermo Fisher dynabeads cd8
    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and <t>CD3</t> on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 <t>Dynabeads,</t> generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).
    Dynabeads Cd8, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads cd8/product/Thermo Fisher
    Average 95 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    dynabeads cd8 - by Bioz Stars, 2020-05
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    94
    Thermo Fisher dynabeads cd45
    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and <t>CD3</t> on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 <t>Dynabeads,</t> generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).
    Dynabeads Cd45, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads cd45/product/Thermo Fisher
    Average 94 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    dynabeads cd45 - by Bioz Stars, 2020-05
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    98
    Thermo Fisher dynabeads myone
    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and <t>CD3</t> on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 <t>Dynabeads,</t> generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).
    Dynabeads Myone, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabeads myone/product/Thermo Fisher
    Average 98 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    dynabeads myone - by Bioz Stars, 2020-05
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    Image Search Results


    The functional response to PD-1/CTLA-4 blockade associate directly with CTLA-4 expression but not FoxP3 + Tregs. (A) Correlation between HCV specific effector cytokine response to combined PD-1/CTLA-4 blockade and ex vivo %CTLA-4 + /CD8 but not %PD-1 + /CD8 and %FoxP3 + /CD4. The y-axis represents the sum of CD8 T cells with HCV-specific IFN-γ + TNF-α + , IFN-γ + TNF-α − and IFN-γ − TNF-α + expression during combined PD-1/CTLA-4 blockade from 14 HLA-A2 − patients (6 intrahepatic and 8 peripheral blood responses). R and p-values by the Spearman rank correlation test. (B) Positive correlation between fold expansion of HCV-specific tetramer + CD8 T cells with combined PD-1/CTLA-4 blockade (relative to PD-1 blockade alone) and ex vivo %CTLA-4 + in HCV-specific tetramer+ CD8 T cells in 7 HLA A2+ HCV-infected patients. R- and p-values by the Spearman rank correlation test. (C) (Left) : Liver infiltrating lymphocytes from chronic patient C07 are examined for CD4, CD8 and FoxP3 + T cell subsets before (upper) and after (lower) depletion of CD4 T cells by CD4 Dynabeads (Dynal Inc), resulting in > 99% depletion of CD4 T cells including FoxP3 + CD4 T cells. (Right) : Undepleted and CD4-depleted liver infiltrating lymphocytes were cultured for 7 days with overlapping HCV NS3-derived 15mer peptides (pNS3) in the presence of isotype or blocking antibodies before direct staining for T cell subsets (CD4, FoxP3) and following additional 6 hours of stimulation with media alone (negative control) or pNS3 peptides to examine HCV-specific intracellular IFN-γ and TNF-α expression in CD8 T cells. Combined PD-1/CTLA-4 blockade promoted markedly enhanced HCV-specific cytokine response in undepleted and CD4-depleted cultures regardless of FoxP3 + Tregs.

    Journal: PLoS Pathogens

    Article Title: Synergistic Reversal of Intrahepatic HCV-Specific CD8 T Cell Exhaustion by Combined PD-1/CTLA-4 Blockade

    doi: 10.1371/journal.ppat.1000313

    Figure Lengend Snippet: The functional response to PD-1/CTLA-4 blockade associate directly with CTLA-4 expression but not FoxP3 + Tregs. (A) Correlation between HCV specific effector cytokine response to combined PD-1/CTLA-4 blockade and ex vivo %CTLA-4 + /CD8 but not %PD-1 + /CD8 and %FoxP3 + /CD4. The y-axis represents the sum of CD8 T cells with HCV-specific IFN-γ + TNF-α + , IFN-γ + TNF-α − and IFN-γ − TNF-α + expression during combined PD-1/CTLA-4 blockade from 14 HLA-A2 − patients (6 intrahepatic and 8 peripheral blood responses). R and p-values by the Spearman rank correlation test. (B) Positive correlation between fold expansion of HCV-specific tetramer + CD8 T cells with combined PD-1/CTLA-4 blockade (relative to PD-1 blockade alone) and ex vivo %CTLA-4 + in HCV-specific tetramer+ CD8 T cells in 7 HLA A2+ HCV-infected patients. R- and p-values by the Spearman rank correlation test. (C) (Left) : Liver infiltrating lymphocytes from chronic patient C07 are examined for CD4, CD8 and FoxP3 + T cell subsets before (upper) and after (lower) depletion of CD4 T cells by CD4 Dynabeads (Dynal Inc), resulting in > 99% depletion of CD4 T cells including FoxP3 + CD4 T cells. (Right) : Undepleted and CD4-depleted liver infiltrating lymphocytes were cultured for 7 days with overlapping HCV NS3-derived 15mer peptides (pNS3) in the presence of isotype or blocking antibodies before direct staining for T cell subsets (CD4, FoxP3) and following additional 6 hours of stimulation with media alone (negative control) or pNS3 peptides to examine HCV-specific intracellular IFN-γ and TNF-α expression in CD8 T cells. Combined PD-1/CTLA-4 blockade promoted markedly enhanced HCV-specific cytokine response in undepleted and CD4-depleted cultures regardless of FoxP3 + Tregs.

    Article Snippet: Depletion of CD4 and CD28 CD4 T cells were depleted from PBL and/or LIL using CD4 Dynabeads (Invitrogen, Oslo, Norway) as previously described .

    Techniques: Functional Assay, Expressing, Ex Vivo, Infection, Cell Culture, Derivative Assay, Blocking Assay, Staining, Negative Control

    Optimization of TRAP immunoprecipitation. The amount of antibody required for the TRAP IP was determined by titration. 500 μl hippocampal lysate was incubated with varying amounts of anti-HA antibody from 2.5 to 12.5 μg, followed by pull down of the antibody-ribosome-mRNA complex with protein A dynabeads. After washing, half the beads were used for protein extraction while the other half were used RNA isolation. ( A ) Protein concentration in the input lysate and remaining in the supernatant after IP (Post-IP Lysate) as well as the amount of protein eluted from the beads was determined by Western blotting. Western blots for the ribosomal protein RPP0 and the HA-tagged RPL22 ribosomal protein are shown (i) along with the quantification of the protein bands (ii). ( B ) Concentration of RNA eluted from the beads is shown for each IP. Based on these combined results 10 μg antibody per 500 μl lysate was determined to be the optimal concentration to ensure maximal depletion of the HA-tagged polysomes and give the best signal-to-noise.

    Journal: eLife

    Article Title: FMRP has a cell-type-specific role in CA1 pyramidal neurons to regulate autism-related transcripts and circadian memory

    doi: 10.7554/eLife.46919

    Figure Lengend Snippet: Optimization of TRAP immunoprecipitation. The amount of antibody required for the TRAP IP was determined by titration. 500 μl hippocampal lysate was incubated with varying amounts of anti-HA antibody from 2.5 to 12.5 μg, followed by pull down of the antibody-ribosome-mRNA complex with protein A dynabeads. After washing, half the beads were used for protein extraction while the other half were used RNA isolation. ( A ) Protein concentration in the input lysate and remaining in the supernatant after IP (Post-IP Lysate) as well as the amount of protein eluted from the beads was determined by Western blotting. Western blots for the ribosomal protein RPP0 and the HA-tagged RPL22 ribosomal protein are shown (i) along with the quantification of the protein bands (ii). ( B ) Concentration of RNA eluted from the beads is shown for each IP. Based on these combined results 10 μg antibody per 500 μl lysate was determined to be the optimal concentration to ensure maximal depletion of the HA-tagged polysomes and give the best signal-to-noise.

    Article Snippet: The remaining lysates was precleared by incubating with 50 μl Protein A Dynabeads (Invitrogen) for 45 min at 4°C with rotation then incubated with 20 μg anti-HA (ab9110, abcam) for 2 hr at 4°C with rotation.

    Techniques: Immunoprecipitation, Titration, Incubation, Protein Extraction, Isolation, Protein Concentration, Western Blot, Concentration Assay

    Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and CD3 on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 Dynabeads, generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).

    Journal: PLoS ONE

    Article Title: Enhanced Cytotoxicity of Natural Killer Cells following the Acquisition of Chimeric Antigen Receptors through Trogocytosis

    doi: 10.1371/journal.pone.0109352

    Figure Lengend Snippet: Immunophenotypic features of expanded NK cells (acceptor cells) and K562-antiCD19BBζ cells (feeder cells). A. Expression of CD56 and CD3 on peripheral blood mononuclear cells from a healthy donor was examined after 1 week (top row) of co-culture with irradiated (IR, left column) or freeze/thaw-treated (F, right column) K562-mb15-41BBL cells at a low dose (10 U/mL) of IL-2. The T cells were removed using CD3 Dynabeads, generating cell populations comprising > 95% CD56+CD3- NK cells (bottom row). B. Percentage of CD56-positive cells within NK cells expanded by co-culturing with irradiated (IR) or freeze/thaw-treated (F) K562-mb15-41BBL cells prior to and after CD3 depletion on day 7. The data are presented as the mean of values obtained using 3 unrelated NK donors. Error bars represent the SD. C. Histogram illustrating the anti-CD19 expression on K562 cells (control, shaded histogram) and K562-antiCD19BBζ cells (feeder cells, open histogram).

    Article Snippet: After 7 days of co-culture, the T cells were removed using CD3 Dynabeads (Invitrogen, Carlsbad, CA), which yielded cell populations containing > 95% CD56+CD3- NK cells.

    Techniques: Expressing, Co-Culture Assay, Irradiation