dynabead m-280 streptavidin Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher streptavidin dynabeads m280
    A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, <t>M280</t> and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, <t>streptavidin</t> ChIP with M280 <t>Dynabeads</t> gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.
    Streptavidin Dynabeads M280, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin dynabeads m280/product/Thermo Fisher
    Average 99 stars, based on 170 article reviews
    Price from $9.99 to $1999.99
    streptavidin dynabeads m280 - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    Igen International dynabead m 280 streptavidin
    A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, <t>M280</t> and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, <t>streptavidin</t> ChIP with M280 <t>Dynabeads</t> gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.
    Dynabead M 280 Streptavidin, supplied by Igen International, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabead m 280 streptavidin/product/Igen International
    Average 88 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    dynabead m 280 streptavidin - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    93
    Thermo Fisher preblocked dynabead m 280 streptavidin
    A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, <t>M280</t> and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, <t>streptavidin</t> ChIP with M280 <t>Dynabeads</t> gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.
    Preblocked Dynabead M 280 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preblocked dynabead m 280 streptavidin/product/Thermo Fisher
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    preblocked dynabead m 280 streptavidin - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    99
    Thermo Fisher dynabead m 280 streptavidin
    A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, <t>M280</t> and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, <t>streptavidin</t> ChIP with M280 <t>Dynabeads</t> gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.
    Dynabead M 280 Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dynabead m 280 streptavidin/product/Thermo Fisher
    Average 99 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    dynabead m 280 streptavidin - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, M280 and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, streptavidin ChIP with M280 Dynabeads gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.

    Journal: BMC Molecular Biology

    Article Title: Optimal use of tandem biotin and V5 tags in ChIP assays

    doi: 10.1186/1471-2199-10-6

    Figure Lengend Snippet: A) Schematic representation of the C-terminally biotin-V5-tagged GATA-1 . NZF and CZF: N-terminal and C-terminal zinc fingers, respectively. V5 and biotin (BIO) tags are not drawn to scale. B) Western blot detected with anti-GATA-1 N6 antibody showing the relative amounts of biotin-V5-GATA-1 and endogenous GATA-1 in the cells used for ChIP. C) Comparison of V5, M280 and two different anti-GATA-1 antibodies, N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. The enrichment is calculated over a BirA-only transfected control or over IgG negative control, respectively. V5 ChIP gives the highest yield in the EKLF enhancer and promoter elements, streptavidin ChIP with M280 Dynabeads gives comparable yield in the upstream enhancer element as M20 anti-GATA-1 antibody. The M20 antibody can enrich for more GATA-1 bound to the EKLF promoter than the M280 Dynabeads. The N6 antibody precipitates the least amount of GATA-1 bound to EKLF promoter elements. D) Comparison of V5, M280 and two different anti-GATA-1 antibodies N6 and M20, tested for the binding of GATA-1 to the EKLF promoter. Enrichment of the specific binding to EKLF promoter and enhancer was calculated over the negative primer set (-1.35 kb element in EKLF promoter). V5 agarose and M280 Dynabeads bring down comparable amounts of GATA-1 bound to EKLF enhancer and promoter sequences. The M20 antibody enriches the most for GATA-1 bound to the EKLF upstream enhancer, though this also included sequences in vivo bound by endogenous GATA-1 protein which can not be bound by M280 or anti-V5 beads. Rat and goat IgGs as well as BirA control show similarly low enrichments of specific primer sets.

    Article Snippet: Streptavidin ChIP Chromatin pull-downs with streptavidin beads were carried out overnight using 20 μl of streptavidin Dynabeads M280 (Invitrogen) or 20 μl of UltraLink Immobilized NeutrAvidin Protein (Pierce) per chromatin aliquot.

    Techniques: Zinc-Fingers, Western Blot, Chromatin Immunoprecipitation, Binding Assay, Transfection, Negative Control, In Vivo

    A) Location of the ChIP primers in the EKLF gene .

    Journal: BMC Molecular Biology

    Article Title: Optimal use of tandem biotin and V5 tags in ChIP assays

    doi: 10.1186/1471-2199-10-6

    Figure Lengend Snippet: A) Location of the ChIP primers in the EKLF gene . "GATA" boxes indicate GATA-1 binding sites. B) Comparison of different derivatives of immobilized streptavidin: NeutrAvidin, streptavidin agarose, streptavidin mutein and M280 Dynabeads. Relative enrichment for EKLF sequences was calculated over negative control chromatin isolated from cells expressing BirA biotin ligase, but not tagged GATA-1.

    Article Snippet: Streptavidin ChIP Chromatin pull-downs with streptavidin beads were carried out overnight using 20 μl of streptavidin Dynabeads M280 (Invitrogen) or 20 μl of UltraLink Immobilized NeutrAvidin Protein (Pierce) per chromatin aliquot.

    Techniques: Chromatin Immunoprecipitation, Binding Assay, Negative Control, Isolation, Expressing

    (a) Western blot analysis of CTB-, AV- and ST-bound MSC EVs. MSC CM was incubated with CTB, AV or ST followed by incubation with Dynabeads conjugated with Streptavidin. The beads were immobilised with a magnet, washed, denatured and resolved onto polyacrylamide gels before electroblotting onto a nitrocellulose membrane. The membrane was probed with a primary antibody followed by horseradish peroxidase-coupled secondary antibodies against the primary antibody. The blot was then incubated with a chemiluminescent HRP substrate to detect bound primary antibody. (b) 10 µg MSC EV was extracted sequentially with biotinylated CTB and then biotinylated AV or vice versa. After each extraction, the ligand-bound vesicles were removed with Dynabeads ® MyOne Streptavidin T1 and assayed for CD81 by ELISA. The relative level of CD81 in CTB-vesicles before and after extraction with AV, and that in AV-vesicles before and after extraction with CTB were normalized to that in AV-vesicles before CTB extraction. (c) RNA analysis of CTB-, AV- and ST-EVs. CTB-, AV- or ST-binding EVs were isolated as described above and extracted for RNA using Trizol. The pellet in each of extracts was re-suspended in 50 µL of RNase-free water. 10 µL of each RNA solution was resolved on a 15% Novex Tris-borate-EDTA(TBE)-urea gel before staining with ethidium bromide.

    Journal: Journal of Extracellular Vesicles

    Article Title: MSC secretes at least 3 EV types each with a unique permutation of membrane lipid, protein and RNA

    doi: 10.3402/jev.v5.29828

    Figure Lengend Snippet: (a) Western blot analysis of CTB-, AV- and ST-bound MSC EVs. MSC CM was incubated with CTB, AV or ST followed by incubation with Dynabeads conjugated with Streptavidin. The beads were immobilised with a magnet, washed, denatured and resolved onto polyacrylamide gels before electroblotting onto a nitrocellulose membrane. The membrane was probed with a primary antibody followed by horseradish peroxidase-coupled secondary antibodies against the primary antibody. The blot was then incubated with a chemiluminescent HRP substrate to detect bound primary antibody. (b) 10 µg MSC EV was extracted sequentially with biotinylated CTB and then biotinylated AV or vice versa. After each extraction, the ligand-bound vesicles were removed with Dynabeads ® MyOne Streptavidin T1 and assayed for CD81 by ELISA. The relative level of CD81 in CTB-vesicles before and after extraction with AV, and that in AV-vesicles before and after extraction with CTB were normalized to that in AV-vesicles before CTB extraction. (c) RNA analysis of CTB-, AV- and ST-EVs. CTB-, AV- or ST-binding EVs were isolated as described above and extracted for RNA using Trizol. The pellet in each of extracts was re-suspended in 50 µL of RNase-free water. 10 µL of each RNA solution was resolved on a 15% Novex Tris-borate-EDTA(TBE)-urea gel before staining with ethidium bromide.

    Article Snippet: The CTB, AV or ST reaction mix was added to 30 µL equivalent of Dynabeads M280 Streptavidin (Thermo Fisher Scientific, Waltham, MA) that were pre-washed as per manufacturer's instruction.

    Techniques: Western Blot, CtB Assay, Incubation, Enzyme-linked Immunosorbent Assay, Binding Assay, Isolation, Staining

    (a) SDS-PAGE analysis of MSC EVs extracted with membrane lipid-binding ligands, CTB, AV and ST, respectively. MSC CM was incubated with CTB, AV or ST followed by incubation with Dynabeads conjugated with Streptavidin. The beads were immobilized with a magnet and the supernatant was collected as the “unbound” fraction. The beads were then washed twice and the wash solutions were collected as “wash 1” and “wash 2,” respectively. The beads were re-suspended in PBS as the “bound” fraction. The equivalent of 20% of the starting samples (input) and each of their respective “unbound,” “wash 1,” “wash 2” and “bound” fractions were resolved onto polyacrylamide gels and the gels were stained with silver. (b) Size distribution of MSC EVs by NanoSight. MSC EVs were diluted 1,000× with 0.22 µm filtered PBS. The size distribution of exosome was then measured using NanoSight LM10 and analysed by Nanoparticles Tracking Analysis software according to the manufacturer's protocol. (c) SEM analysis of MSC EVs that were extracted with CTB, AV and ST. MSC EV preparation was incubated with biotinylated CTB, AV, ST or without ligand and then streptavidin-coated polystyrene particles. The beads were then washed twice with PBS and resuspended in PBS before being spotted and left to dry onto carbon tape on aluminium stubs at 40°C. The stubs were sputter coated with 2 ηm of gold coating (Leica Biosystems) and imaged in a Jeol 6701FESEM. Scale bar=100 ηm.

    Journal: Journal of Extracellular Vesicles

    Article Title: MSC secretes at least 3 EV types each with a unique permutation of membrane lipid, protein and RNA

    doi: 10.3402/jev.v5.29828

    Figure Lengend Snippet: (a) SDS-PAGE analysis of MSC EVs extracted with membrane lipid-binding ligands, CTB, AV and ST, respectively. MSC CM was incubated with CTB, AV or ST followed by incubation with Dynabeads conjugated with Streptavidin. The beads were immobilized with a magnet and the supernatant was collected as the “unbound” fraction. The beads were then washed twice and the wash solutions were collected as “wash 1” and “wash 2,” respectively. The beads were re-suspended in PBS as the “bound” fraction. The equivalent of 20% of the starting samples (input) and each of their respective “unbound,” “wash 1,” “wash 2” and “bound” fractions were resolved onto polyacrylamide gels and the gels were stained with silver. (b) Size distribution of MSC EVs by NanoSight. MSC EVs were diluted 1,000× with 0.22 µm filtered PBS. The size distribution of exosome was then measured using NanoSight LM10 and analysed by Nanoparticles Tracking Analysis software according to the manufacturer's protocol. (c) SEM analysis of MSC EVs that were extracted with CTB, AV and ST. MSC EV preparation was incubated with biotinylated CTB, AV, ST or without ligand and then streptavidin-coated polystyrene particles. The beads were then washed twice with PBS and resuspended in PBS before being spotted and left to dry onto carbon tape on aluminium stubs at 40°C. The stubs were sputter coated with 2 ηm of gold coating (Leica Biosystems) and imaged in a Jeol 6701FESEM. Scale bar=100 ηm.

    Article Snippet: The CTB, AV or ST reaction mix was added to 30 µL equivalent of Dynabeads M280 Streptavidin (Thermo Fisher Scientific, Waltham, MA) that were pre-washed as per manufacturer's instruction.

    Techniques: SDS Page, Binding Assay, CtB Assay, Incubation, Staining, Software

    Recruitment of the transcription machinery into the PIC. (A) Complexes were assembled on M280-streptavidin Dynabeads carrying a biotinylated DNA fragment from the plasmid pA4xMLΔ53, which contained four copies of the HNF-4 cognate site A and the adenovirus major late core promoter. After PIC formation, beads were washed and bound complexes were analyzed for their factor content by immunoblotting. PICs were formed with GTFs only (TBP, TFIIB, TFIIE, TFIIF, TFIIH, Pol II (lane 3); with GTFs and HNF-4 (lane 4); or with GTFs, HNF-4, and PC4 (lanes 2 and 5). Lane 2, control in which the indicated factors were incubated with beads only (no template DNA); lane 1, GTFs only (input). (B) Immobilized-template recruitment assays are as for panel A, except that all reaction mixtures contained TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, Pol II, and TRAP/SMCC/Mediator, as shown in input lane 1. HNF-4 (lanes 3 and 5) and PC4 (lanes 4 and 5) were additionally added as indicated. (C) Immobilized-template recruitment assays are as for panel B, except that reaction mixtures in lanes 6 and 7 did not contain TFIID. HNF-4 (lanes 3, 5, and 7) and PC4 (lanes 4 to 7) were additionally added as indicated.

    Journal: Molecular and Cellular Biology

    Article Title: TRAP/SMCC/Mediator-Dependent Transcriptional Activation from DNA and Chromatin Templates by Orphan Nuclear Receptor Hepatocyte Nuclear Factor 4

    doi: 10.1128/MCB.22.15.5626-5637.2002

    Figure Lengend Snippet: Recruitment of the transcription machinery into the PIC. (A) Complexes were assembled on M280-streptavidin Dynabeads carrying a biotinylated DNA fragment from the plasmid pA4xMLΔ53, which contained four copies of the HNF-4 cognate site A and the adenovirus major late core promoter. After PIC formation, beads were washed and bound complexes were analyzed for their factor content by immunoblotting. PICs were formed with GTFs only (TBP, TFIIB, TFIIE, TFIIF, TFIIH, Pol II (lane 3); with GTFs and HNF-4 (lane 4); or with GTFs, HNF-4, and PC4 (lanes 2 and 5). Lane 2, control in which the indicated factors were incubated with beads only (no template DNA); lane 1, GTFs only (input). (B) Immobilized-template recruitment assays are as for panel A, except that all reaction mixtures contained TFIIA, TFIIB, TFIID, TFIIE, TFIIF, TFIIH, Pol II, and TRAP/SMCC/Mediator, as shown in input lane 1. HNF-4 (lanes 3 and 5) and PC4 (lanes 4 and 5) were additionally added as indicated. (C) Immobilized-template recruitment assays are as for panel B, except that reaction mixtures in lanes 6 and 7 did not contain TFIID. HNF-4 (lanes 3, 5, and 7) and PC4 (lanes 4 to 7) were additionally added as indicated.

    Article Snippet: 600 bp) from plasmid pA4xMLΔ53 ( , ), which contains the HNF-4 cognate sites and the core promoter elements, was filled in with biotinylated dATP by using the Klenow fragment of DNA Pol I, gel purified, and bound to M280-streptavidin Dynabeads (Dynal), as suggested by the manufacturer.

    Techniques: Plasmid Preparation, Incubation

    Quantitative analyses of DS-bound proteins after release with DNase I digestion. A 428-bp DNA fragment (ARV) bearing DS was biotinylated at both ends and immobilized on streptavidin-Dynabeads. Then EB90 mbp 323 (11 pmol), Orc1–5 complex (0.6 pmol),

    Journal: The Journal of Biological Chemistry

    Article Title: Epstein-Barr Nuclear Antigen 1 (EBNA1)-dependent Recruitment of Origin Recognition Complex (Orc) on oriP of Epstein-Barr Virus with Purified Proteins

    doi: 10.1074/jbc.M112.368456

    Figure Lengend Snippet: Quantitative analyses of DS-bound proteins after release with DNase I digestion. A 428-bp DNA fragment (ARV) bearing DS was biotinylated at both ends and immobilized on streptavidin-Dynabeads. Then EB90 mbp 323 (11 pmol), Orc1–5 complex (0.6 pmol),

    Article Snippet: Dynabeads-streptavidin M280 was obtained from Invitrogen.

    Techniques: