dye jc1 Search Results


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  • 99
    Thermo Fisher jc1 dye
    Disruption of calcium homeostasis and alteration of mitochondrial potential for selected drugs and drug pairs. ( A ) Time lapsed capture of calcium dependent Fura −2 fluorescence for two live side-by-side intraerythrocytic strain Dd2 parasites showing rapid loss of digestive vacuole (D.V.) Ca 2+ (bright green inner circle, top panels) upon perfusion with cytocidal (2 × LD 50 ) dose of CQ (see methods). ( B ) Examination of the combination responses of KN-62 and Artemether (ATM) in three parasite lines via an isobologram analysis of the mitochondrial membrane potential as judged by a combination <t>JC1</t> assay (panel 1), heatmap analysis of the viability combination response (10 × 10 matrix) (panel 2), Delta Bliss analysis (panel 3), and isobolographic analysis of the viability combination response (panel 4).
    Jc1 Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore jc1 dye
    Disruption of calcium homeostasis and alteration of mitochondrial potential for selected drugs and drug pairs. ( A ) Time lapsed capture of calcium dependent Fura −2 fluorescence for two live side-by-side intraerythrocytic strain Dd2 parasites showing rapid loss of digestive vacuole (D.V.) Ca 2+ (bright green inner circle, top panels) upon perfusion with cytocidal (2 × LD 50 ) dose of CQ (see methods). ( B ) Examination of the combination responses of KN-62 and Artemether (ATM) in three parasite lines via an isobologram analysis of the mitochondrial membrane potential as judged by a combination <t>JC1</t> assay (panel 1), heatmap analysis of the viability combination response (10 × 10 matrix) (panel 2), Delta Bliss analysis (panel 3), and isobolographic analysis of the viability combination response (panel 4).
    Jc1 Dye, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Beyotime dye jc 1
    Flow cytometry of ROS generation and mitochondria depolarization in UVA irradiated HDFs after co-culturing with or without HAMSCs for 72 h or without. Transwells containing HAMSCs were moved into the correlating wells containing HDFs which just accepted 9 J/cm 2 UVA irradiation. (A) Macrogaphs of DCFDA fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culturing with/without HAMSCs. (B) <t>JC-1</t> fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culture with or without HAMSCs. ## P
    Dye Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 89/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Keygen Biotech dye jc 1
    AOPP s triggered intrinsic apoptosis pathway by  NADPH  oxidase‐dependent,  MAPK  signaling. (a) Confocal microscopy analysis using  JC ‐1 staining revealed that  AOPP  challenge for 24 hr decreased the ▵Ψm level of  MC 3T3‐E1 cells in a dose‐dependent manner (Bar = 100 μm). Numerical data were expressed in terms of the ratio of  JC ‐1 aggregates to  JC ‐1 monomers, the increased ration stand for the decreased ▵Ψm. (b–g)  AOPP  treatment (200 μg/ml) significantly increased the expression of  BAX , cytochrome c, cleaved caspase‐9, cleaved caspase‐12, BiP/ GRP 78, phosphorylated Ca 2+  channel  IP 3R, cleaved caspase‐3, and cleaved  PARP , while decreased the expression of Bcl‐2, intact caspase‐9, intact caspase‐3, and intact  PARP  in 48 hr. (h,i) Pretreated with apocynin (100 μ m ),  DPI  (10 μ m ), and  SOD  (50 U/ml) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐3, cleaved  PARP , cleaved caspase‐9,  BAX , cleaved caspase‐12, and BiP/ GRP 78, while increased the expression National Natural Science Foundation of Bcl‐2. (m) p47 phox  lentiviral  RNA i vector transfection significantly decreased  AOPP ‐induced expression of cleaved caspase‐3 and cleaved  PARP . (j–l)  JNK  inhibitor  SP 600125 (10 μ m ), p38 inhibitor  SB 203580 (10 μ m ), and  ERK  inhibitor U0126 (10 μ m ) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐9,  BAX , cleaved caspase‐12, BiP/ GRP 78, cleaved caspase‐3, and cleaved  PARP . (n) Intracellular calcium level was determined by Fluo‐3/ AM .  AOPP  (200 μg/ml) treatment induced Ca 2+  overload in a time‐dependent manner, while this effect could be blocked by  IP 3R inhibitor Xestospongin C (5 μ m ) and  NADPH  oxidase inhibitor apocynin (100 μ m ). (o) Pretreated with caspase inhibitor Z‐ VAD ‐ FMK  (20 μ m ) and  IP 3R inhibitor Xestospongin C (5 μ m ) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) cleaved  PARP  expression. Cells in the inhibitor group were pretreated with apocynin,  DPI ,  SOD ,  SP 600125,  SB 203580, U0126, Xestospongin C, and Z‐ VAD ‐ FMK , respectively, for 40 min before  AOPP  administration, and all of them were present during  AOPP  incubation. Data were presented as mean ±  SD . * p
    Dye Jc 1, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nanjing KeyGen Biotech Co Ltd dye jc 1
    Nec-1 treatment decreases the Δψm of C2C12 cells under hypoxic conditions. (A) Flow cytometry images and (B) statistical analysis of <t>JC-1.</t> The x-axis represents monomer intensity, whereas the y-axis indicates the aggregate intensity. * P
    Dye Jc 1, supplied by Nanjing KeyGen Biotech Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Cell Technology Inc ratiometric mitochondrial dye jc1
    Nec-1 treatment decreases the Δψm of C2C12 cells under hypoxic conditions. (A) Flow cytometry images and (B) statistical analysis of <t>JC-1.</t> The x-axis represents monomer intensity, whereas the y-axis indicates the aggregate intensity. * P
    Ratiometric Mitochondrial Dye Jc1, supplied by Cell Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Biotium jc1 dye
    Aged NPCs have fewer mitochondria and are resistant to rotenone toxicity. A and B , numbers of <t>JC1</t> + mitochondria ( red ) were quantified in young adult ( A ) and aged adult ( B ) NPCs. Scale bars , 5 μm. C , a significant decrease in JC1 + mitochondria/cell
    Jc1 Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Beyotime cationic dye jc 1
    Involvement of mitochondrial apoptotic pathway in radiosensitivity after autophagy inhibition in Eca-109 cells. At 12 h after treatment with IR (8 Gy) with or without pretreatment with autophagy inhibitors (10 μ M for LY294002 and 1 mM for 3-MA), cytochrome c release (A) and Bax translocation to mitochondria (B), and the cellular apoptosis initiators caspase-8 and caspase-9, the effector caspase-3, and the apoptotic protein Bcl-2 (C) in Eca-109 cells were analyzed by western blotting. GAPDH and COX IV were used as internal protein loading controls for the cytosolic and mitochondrial fractions, respectively. (D and E) After treatment as in A, representative flow cytometric analysis of <t>JC-1</t> assay was conducted, and the depolarized cells exhibited decreased red fluorescence and enhanced green fluorescence. The histogram presents the change of green fluorescence intensity in Eca-109 cells after various treatments (mean ± standard deviation, n=3, * P
    Cationic Dye Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Biotium cationic dye jc 1
    Bornyl cis -4-hydroxycinnamate induced apoptosis through mitochondria potential (Δψm) change and the mitochondrial-mediated pathway in A2058 and A375 melanoma cells. ( A ) A2058 and A375 melanoma cells were treated with or without bornyl cis -4-hydroxycinnamate; Δψm in melanoma cells was detected by <t>JC-1</t> staining and analyzed using fluorescence microscopy. Scale bar: 50 μm. ( B ) Changes in Bcl-2, Bcl-xl, Mcl-1, Bad, p-Bad, Bax, and cytosolic cytochrome C expression in two melanoma cells treated with different concentrations of bornyl cis -4-hydroxycinnamate visualized by western blotting analysis. β-actin was used as the internal control.
    Cationic Dye Jc 1, supplied by Biotium, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Enzo Biochem fluorescent dye jc 1
    TAS restored mitochondrial transmembrane potential. HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. Mitochondrial membrane potential was determined with <t>JC-1</t> staining, which was observed using a fluorescence microscope. Image of fluorescence microscope on mitochondrial membrane potential (MMP) was obtained with 200 X of magnification.
    Fluorescent Dye Jc 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher ratiometric dye jc 1
    TAS restored mitochondrial transmembrane potential. HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. Mitochondrial membrane potential was determined with <t>JC-1</t> staining, which was observed using a fluorescence microscope. Image of fluorescence microscope on mitochondrial membrane potential (MMP) was obtained with 200 X of magnification.
    Ratiometric Dye Jc 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime cyanine dye jc 1
    TAS restored mitochondrial transmembrane potential. HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. Mitochondrial membrane potential was determined with <t>JC-1</t> staining, which was observed using a fluorescence microscope. Image of fluorescence microscope on mitochondrial membrane potential (MMP) was obtained with 200 X of magnification.
    Cyanine Dye Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beyotime fluorescent dye jc 1
    Effects of DZX on regulating the ΔYm, cleaved caspase 3 expression and caspase 3 activities in cultured cardiomyocytes. (A) Detection of ΔYm in the five groups of cardiomyocytes by fluorescent dye <t>JC-1.</t> Scale bar, 200 µ m. (B) Comparison of ΔYm in the five groups of cardiomyocytes. For quantification, 50 cells were randomly selected to calculate the ΔYm levels by comparing red fluorescent intensity to green fluorescent intensity. (C) Western blot images of cleaved caspase 3 expression. (D) Semi-quantitative analysis of cleaved caspase 3 expression (n=5). (E) Detection of caspase 3 activity in the five groups of cardiomyocytes. The caspase 3 activity was calculated by p-nitroaniline concentration relative to total protein concentration (n=3-6). The data are presented as mean ± standard deviation. * P
    Fluorescent Dye Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    ChemoMetec cationic dye jc 1
    Effects of DZX on regulating the ΔYm, cleaved caspase 3 expression and caspase 3 activities in cultured cardiomyocytes. (A) Detection of ΔYm in the five groups of cardiomyocytes by fluorescent dye <t>JC-1.</t> Scale bar, 200 µ m. (B) Comparison of ΔYm in the five groups of cardiomyocytes. For quantification, 50 cells were randomly selected to calculate the ΔYm levels by comparing red fluorescent intensity to green fluorescent intensity. (C) Western blot images of cleaved caspase 3 expression. (D) Semi-quantitative analysis of cleaved caspase 3 expression (n=5). (E) Detection of caspase 3 activity in the five groups of cardiomyocytes. The caspase 3 activity was calculated by p-nitroaniline concentration relative to total protein concentration (n=3-6). The data are presented as mean ± standard deviation. * P
    Cationic Dye Jc 1, supplied by ChemoMetec, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Enzo Biochem cationic dye jc 1
    Effects of DZX on regulating the ΔYm, cleaved caspase 3 expression and caspase 3 activities in cultured cardiomyocytes. (A) Detection of ΔYm in the five groups of cardiomyocytes by fluorescent dye <t>JC-1.</t> Scale bar, 200 µ m. (B) Comparison of ΔYm in the five groups of cardiomyocytes. For quantification, 50 cells were randomly selected to calculate the ΔYm levels by comparing red fluorescent intensity to green fluorescent intensity. (C) Western blot images of cleaved caspase 3 expression. (D) Semi-quantitative analysis of cleaved caspase 3 expression (n=5). (E) Detection of caspase 3 activity in the five groups of cardiomyocytes. The caspase 3 activity was calculated by p-nitroaniline concentration relative to total protein concentration (n=3-6). The data are presented as mean ± standard deviation. * P
    Cationic Dye Jc 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Enzo Biochem fluorescence dye jc 1
    Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by <t>JC-1</t> staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p
    Fluorescence Dye Jc 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime cationic lipophilic dye jc 1
    Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by <t>JC-1</t> staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p
    Cationic Lipophilic Dye Jc 1, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime dye jc 1 assay kit
    Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by <t>JC-1</t> staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p
    Dye Jc 1 Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Tecan Systems dye jc 1
    Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by <t>JC-1</t> staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p
    Dye Jc 1, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Disruption of calcium homeostasis and alteration of mitochondrial potential for selected drugs and drug pairs. ( A ) Time lapsed capture of calcium dependent Fura −2 fluorescence for two live side-by-side intraerythrocytic strain Dd2 parasites showing rapid loss of digestive vacuole (D.V.) Ca 2+ (bright green inner circle, top panels) upon perfusion with cytocidal (2 × LD 50 ) dose of CQ (see methods). ( B ) Examination of the combination responses of KN-62 and Artemether (ATM) in three parasite lines via an isobologram analysis of the mitochondrial membrane potential as judged by a combination JC1 assay (panel 1), heatmap analysis of the viability combination response (10 × 10 matrix) (panel 2), Delta Bliss analysis (panel 3), and isobolographic analysis of the viability combination response (panel 4).

    Journal: Scientific Reports

    Article Title: High-throughput matrix screening identifies synergistic and antagonistic antimalarial drug combinations

    doi: 10.1038/srep13891

    Figure Lengend Snippet: Disruption of calcium homeostasis and alteration of mitochondrial potential for selected drugs and drug pairs. ( A ) Time lapsed capture of calcium dependent Fura −2 fluorescence for two live side-by-side intraerythrocytic strain Dd2 parasites showing rapid loss of digestive vacuole (D.V.) Ca 2+ (bright green inner circle, top panels) upon perfusion with cytocidal (2 × LD 50 ) dose of CQ (see methods). ( B ) Examination of the combination responses of KN-62 and Artemether (ATM) in three parasite lines via an isobologram analysis of the mitochondrial membrane potential as judged by a combination JC1 assay (panel 1), heatmap analysis of the viability combination response (10 × 10 matrix) (panel 2), Delta Bliss analysis (panel 3), and isobolographic analysis of the viability combination response (panel 4).

    Article Snippet: The supernatant was removed and cells were resuspended in 50 μl of 0.9% NaCl/0.2% Dextrose (Baxter Healthcare, Deerfield, IL) with 2 μM JC1 dye (Invitrogen) and 1 μM Syto61 dye (Invitrogen).

    Techniques: Fluorescence

    EV71 infection causes decline in ΔΨ m . SF268 cells were mock- (A) or infected with (B) EV71 at an m.o.i. of 1.25 for 48 hr. Cell were stained with JC-1 and Hoechst 33342 dyes, and examined by confocal microscopy. Intracellular distribution of JC-1 J-aggregate (a) and monomer (b) is indicative of ΔΨ m in cells. Nuclei of these cells are shown (c). The corresponding images are overlaid (d). The photographs shown here are representative of three experiments. Scale bar, 20 µm. (C) SF268 cells were mock- or infected with EV71 at an m.o.i. of 1.25 for indicated times, and were subject to JC-1 staining and flow cytometric analysis. The ratio of MRI of FL2 channel to that of FL1 channel (FL2/FL1) was calculated, and is expressed relative to that of uninfected cells. Results are mean ± SD, n = 3. *p

    Journal: PLoS ONE

    Article Title: Enterovirus 71 Induces Mitochondrial Reactive Oxygen Species Generation That is Required for Efficient Replication

    doi: 10.1371/journal.pone.0113234

    Figure Lengend Snippet: EV71 infection causes decline in ΔΨ m . SF268 cells were mock- (A) or infected with (B) EV71 at an m.o.i. of 1.25 for 48 hr. Cell were stained with JC-1 and Hoechst 33342 dyes, and examined by confocal microscopy. Intracellular distribution of JC-1 J-aggregate (a) and monomer (b) is indicative of ΔΨ m in cells. Nuclei of these cells are shown (c). The corresponding images are overlaid (d). The photographs shown here are representative of three experiments. Scale bar, 20 µm. (C) SF268 cells were mock- or infected with EV71 at an m.o.i. of 1.25 for indicated times, and were subject to JC-1 staining and flow cytometric analysis. The ratio of MRI of FL2 channel to that of FL1 channel (FL2/FL1) was calculated, and is expressed relative to that of uninfected cells. Results are mean ± SD, n = 3. *p

    Article Snippet: The mitochondrial membrane potential was determined using the cationic, lipophilic dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenz- imidazolocarbocyanine iodide) (Invitrogen, CA, USA).

    Techniques: Infection, Staining, Confocal Microscopy, Flow Cytometry, Magnetic Resonance Imaging

    Effect of mitocurcuminoids and curcumin on mitochondrial membrane potential and apoptotic markers. ( A ) Cells were treated with 10 µM Mitocur-1, 2, 3 or 50 µM curcumin for 4 h. Then washed with PBS and incubated with JC-1 dye (5 µg/ml) for 20 min to measure the loss of mitochondrial membrane potential. Fluorescence images were captured in both FITC and rhodamine filters and images showing J-aggregates are represented. ( B ) shows quantification of images (J-aggregates) shown in A. ( C ) Mitochondria and cytosolic fractions were isolated using ProteoExtract Cytosol/Mitochondria Fractionation Kit and cytochrome c levels were measured by Western blot analysis. ( D ) MCF-7 cells were treated with Mitocur-1 (5 and 10 µM) for 24 h. Total protein was resolved by SDS-PAGE electrophoresis and Western blot analysis was performed using respective antibodies for Bcl2, Bax, caspase-7 and PARP. **, significantly different compared to control (p

    Journal: PLoS ONE

    Article Title: Mitochondrial-Targeted Curcuminoids: A Strategy to Enhance Bioavailability and Anticancer Efficacy of Curcumin

    doi: 10.1371/journal.pone.0089351

    Figure Lengend Snippet: Effect of mitocurcuminoids and curcumin on mitochondrial membrane potential and apoptotic markers. ( A ) Cells were treated with 10 µM Mitocur-1, 2, 3 or 50 µM curcumin for 4 h. Then washed with PBS and incubated with JC-1 dye (5 µg/ml) for 20 min to measure the loss of mitochondrial membrane potential. Fluorescence images were captured in both FITC and rhodamine filters and images showing J-aggregates are represented. ( B ) shows quantification of images (J-aggregates) shown in A. ( C ) Mitochondria and cytosolic fractions were isolated using ProteoExtract Cytosol/Mitochondria Fractionation Kit and cytochrome c levels were measured by Western blot analysis. ( D ) MCF-7 cells were treated with Mitocur-1 (5 and 10 µM) for 24 h. Total protein was resolved by SDS-PAGE electrophoresis and Western blot analysis was performed using respective antibodies for Bcl2, Bax, caspase-7 and PARP. **, significantly different compared to control (p

    Article Snippet: Detection of mitochondrial transmembrane potential Mitochondrial potential was assessed using the fluorescent potentiometric dye JC-1 (5,5′,6,6′-tetrachloro-1,1′, 3, 3′-tetraethylbenzimidazolcarbocyanine iodide) (Molecular Probes, Eugene, OR).

    Techniques: Incubation, Fluorescence, Isolation, Fractionation, Western Blot, SDS Page, Electrophoresis

    MMP changes in HeLa cells after treatment with marine bacterial extracts. Cells were treated with 500 μg/mL of each extract for 12 and 16 h and stained with 50 μM JC-1 and analyzed by flow cytometry. Figure presents the 2D plots of FL-2H vs. FL-1H indicating percentage of cells (± S.D) with intact (red) or permeable (green) mitochondrial membranes. Untx represent untreated sample and 100 mM H 2 O 2 was used as a positive control. The Z- factor was calculated to be 0.79 and 0.80 for 12 h and 16 h time points, respectively.

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Induction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts

    doi: 10.1186/1472-6882-13-344

    Figure Lengend Snippet: MMP changes in HeLa cells after treatment with marine bacterial extracts. Cells were treated with 500 μg/mL of each extract for 12 and 16 h and stained with 50 μM JC-1 and analyzed by flow cytometry. Figure presents the 2D plots of FL-2H vs. FL-1H indicating percentage of cells (± S.D) with intact (red) or permeable (green) mitochondrial membranes. Untx represent untreated sample and 100 mM H 2 O 2 was used as a positive control. The Z- factor was calculated to be 0.79 and 0.80 for 12 h and 16 h time points, respectively.

    Article Snippet: Next day, cells were treated with 500 μg/mL marine bacterial extracts for 12 and 16 h and stained with 50 μM cyanine dye JC-1 (5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi- dazolylcarbocyanine iodide, Life Technologies, UK) for 1 h. Cells were analyzed by HTFC system by plotting FL2-H vs. FL-1H and applying a quadrant gate to determine JC-1 aggregates (red) and monomers (green).

    Techniques: Staining, Flow Cytometry, Cytometry, Positive Control

    Flow cytometry of ROS generation and mitochondria depolarization in UVA irradiated HDFs after co-culturing with or without HAMSCs for 72 h or without. Transwells containing HAMSCs were moved into the correlating wells containing HDFs which just accepted 9 J/cm 2 UVA irradiation. (A) Macrogaphs of DCFDA fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culturing with/without HAMSCs. (B) JC-1 fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culture with or without HAMSCs. ## P

    Journal: Molecular Medicine Reports

    Article Title: Human amnion-derived mesenchymal stem cells protect against UVA irradiation-induced human dermal fibroblast senescence, in vitro

    doi: 10.3892/mmr.2017.6795

    Figure Lengend Snippet: Flow cytometry of ROS generation and mitochondria depolarization in UVA irradiated HDFs after co-culturing with or without HAMSCs for 72 h or without. Transwells containing HAMSCs were moved into the correlating wells containing HDFs which just accepted 9 J/cm 2 UVA irradiation. (A) Macrogaphs of DCFDA fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culturing with/without HAMSCs. (B) JC-1 fluorescence was determined by flow cytometry at 72 h after UVA irradiation and co-culture with or without HAMSCs. ## P

    Article Snippet: Flow cytometry analysis of mitochondrial membrane potential Mitochondrial membrane potential (Δψm) was analyzed by a fluorescent dye JC-1 (Beyotime Institute of Biotechnology), following manufactur's protocol.

    Techniques: Flow Cytometry, Cytometry, Irradiation, Fluorescence, Co-Culture Assay

    AOPP s triggered intrinsic apoptosis pathway by  NADPH  oxidase‐dependent,  MAPK  signaling. (a) Confocal microscopy analysis using  JC ‐1 staining revealed that  AOPP  challenge for 24 hr decreased the ▵Ψm level of  MC 3T3‐E1 cells in a dose‐dependent manner (Bar = 100 μm). Numerical data were expressed in terms of the ratio of  JC ‐1 aggregates to  JC ‐1 monomers, the increased ration stand for the decreased ▵Ψm. (b–g)  AOPP  treatment (200 μg/ml) significantly increased the expression of  BAX , cytochrome c, cleaved caspase‐9, cleaved caspase‐12, BiP/ GRP 78, phosphorylated Ca 2+  channel  IP 3R, cleaved caspase‐3, and cleaved  PARP , while decreased the expression of Bcl‐2, intact caspase‐9, intact caspase‐3, and intact  PARP  in 48 hr. (h,i) Pretreated with apocynin (100 μ m ),  DPI  (10 μ m ), and  SOD  (50 U/ml) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐3, cleaved  PARP , cleaved caspase‐9,  BAX , cleaved caspase‐12, and BiP/ GRP 78, while increased the expression National Natural Science Foundation of Bcl‐2. (m) p47 phox  lentiviral  RNA i vector transfection significantly decreased  AOPP ‐induced expression of cleaved caspase‐3 and cleaved  PARP . (j–l)  JNK  inhibitor  SP 600125 (10 μ m ), p38 inhibitor  SB 203580 (10 μ m ), and  ERK  inhibitor U0126 (10 μ m ) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐9,  BAX , cleaved caspase‐12, BiP/ GRP 78, cleaved caspase‐3, and cleaved  PARP . (n) Intracellular calcium level was determined by Fluo‐3/ AM .  AOPP  (200 μg/ml) treatment induced Ca 2+  overload in a time‐dependent manner, while this effect could be blocked by  IP 3R inhibitor Xestospongin C (5 μ m ) and  NADPH  oxidase inhibitor apocynin (100 μ m ). (o) Pretreated with caspase inhibitor Z‐ VAD ‐ FMK  (20 μ m ) and  IP 3R inhibitor Xestospongin C (5 μ m ) significantly decreased  AOPP ‐induced (200 μg/ml, 48 hr) cleaved  PARP  expression. Cells in the inhibitor group were pretreated with apocynin,  DPI ,  SOD ,  SP 600125,  SB 203580, U0126, Xestospongin C, and Z‐ VAD ‐ FMK , respectively, for 40 min before  AOPP  administration, and all of them were present during  AOPP  incubation. Data were presented as mean ±  SD . * p

    Journal: Aging Cell

    Article Title: Advanced oxidation protein products induce pre‐osteoblast apoptosis through a nicotinamide adenine dinucleotide phosphate oxidase‐dependent, mitogen‐activated protein kinases‐mediated intrinsic apoptosis pathway, et al. Advanced oxidation protein products induce pre‐osteoblast apoptosis through a nicotinamide adenine dinucleotide phosphate oxidase‐dependent, mitogen‐activated protein kinases‐mediated intrinsic apoptosis pathway

    doi: 10.1111/acel.12764

    Figure Lengend Snippet: AOPP s triggered intrinsic apoptosis pathway by NADPH oxidase‐dependent, MAPK signaling. (a) Confocal microscopy analysis using JC ‐1 staining revealed that AOPP challenge for 24 hr decreased the ▵Ψm level of MC 3T3‐E1 cells in a dose‐dependent manner (Bar = 100 μm). Numerical data were expressed in terms of the ratio of JC ‐1 aggregates to JC ‐1 monomers, the increased ration stand for the decreased ▵Ψm. (b–g) AOPP treatment (200 μg/ml) significantly increased the expression of BAX , cytochrome c, cleaved caspase‐9, cleaved caspase‐12, BiP/ GRP 78, phosphorylated Ca 2+ channel IP 3R, cleaved caspase‐3, and cleaved PARP , while decreased the expression of Bcl‐2, intact caspase‐9, intact caspase‐3, and intact PARP in 48 hr. (h,i) Pretreated with apocynin (100 μ m ), DPI (10 μ m ), and SOD (50 U/ml) significantly decreased AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐3, cleaved PARP , cleaved caspase‐9, BAX , cleaved caspase‐12, and BiP/ GRP 78, while increased the expression National Natural Science Foundation of Bcl‐2. (m) p47 phox lentiviral RNA i vector transfection significantly decreased AOPP ‐induced expression of cleaved caspase‐3 and cleaved PARP . (j–l) JNK inhibitor SP 600125 (10 μ m ), p38 inhibitor SB 203580 (10 μ m ), and ERK inhibitor U0126 (10 μ m ) significantly decreased AOPP ‐induced (200 μg/ml, 48 hr) expression of cleaved caspase‐9, BAX , cleaved caspase‐12, BiP/ GRP 78, cleaved caspase‐3, and cleaved PARP . (n) Intracellular calcium level was determined by Fluo‐3/ AM . AOPP (200 μg/ml) treatment induced Ca 2+ overload in a time‐dependent manner, while this effect could be blocked by IP 3R inhibitor Xestospongin C (5 μ m ) and NADPH oxidase inhibitor apocynin (100 μ m ). (o) Pretreated with caspase inhibitor Z‐ VAD ‐ FMK (20 μ m ) and IP 3R inhibitor Xestospongin C (5 μ m ) significantly decreased AOPP ‐induced (200 μg/ml, 48 hr) cleaved PARP expression. Cells in the inhibitor group were pretreated with apocynin, DPI , SOD , SP 600125, SB 203580, U0126, Xestospongin C, and Z‐ VAD ‐ FMK , respectively, for 40 min before AOPP administration, and all of them were present during AOPP incubation. Data were presented as mean ±  SD . * p

    Article Snippet: 4.6 Determination of mitochondrial membrane potential The fluorescent dye JC‐1 (KeyGen Biotech, China) was used to assess the change in mitochondrial membrane potential.

    Techniques: Confocal Microscopy, Staining, Expressing, Plasmid Preparation, Transfection, Incubation

    Nec-1 treatment decreases the Δψm of C2C12 cells under hypoxic conditions. (A) Flow cytometry images and (B) statistical analysis of JC-1. The x-axis represents monomer intensity, whereas the y-axis indicates the aggregate intensity. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Necrostatin-1 protects C2C12 myotubes from CoCl2-induced hypoxia

    doi: 10.3892/ijmm.2018.3466

    Figure Lengend Snippet: Nec-1 treatment decreases the Δψm of C2C12 cells under hypoxic conditions. (A) Flow cytometry images and (B) statistical analysis of JC-1. The x-axis represents monomer intensity, whereas the y-axis indicates the aggregate intensity. * P

    Article Snippet: The Δψm of the C2C12 cells was determined by CytoFLEX (Beckman Coulter, Brea, CA, USA) with the fluorescent dye JC-1 (Nanjing KeyGEN Biotech Co., Ltd., Nanjing, China).

    Techniques: Flow Cytometry, Cytometry

    Aged NPCs have fewer mitochondria and are resistant to rotenone toxicity. A and B , numbers of JC1 + mitochondria ( red ) were quantified in young adult ( A ) and aged adult ( B ) NPCs. Scale bars , 5 μm. C , a significant decrease in JC1 + mitochondria/cell

    Journal: The Journal of Biological Chemistry

    Article Title: Aging Neural Progenitor Cells Have Decreased Mitochondrial Content and Lower Oxidative Metabolism *

    doi: 10.1074/jbc.M111.252171

    Figure Lengend Snippet: Aged NPCs have fewer mitochondria and are resistant to rotenone toxicity. A and B , numbers of JC1 + mitochondria ( red ) were quantified in young adult ( A ) and aged adult ( B ) NPCs. Scale bars , 5 μm. C , a significant decrease in JC1 + mitochondria/cell

    Article Snippet: Live cells were plated on poly- l -lysine-coated coverslips and exposed to 1× JC1 dye (Biotium 30001) for 90 min, then coverslipped and photographed at ×60 using confocal fluorescence microscopy.

    Techniques:

    Involvement of mitochondrial apoptotic pathway in radiosensitivity after autophagy inhibition in Eca-109 cells. At 12 h after treatment with IR (8 Gy) with or without pretreatment with autophagy inhibitors (10 μ M for LY294002 and 1 mM for 3-MA), cytochrome c release (A) and Bax translocation to mitochondria (B), and the cellular apoptosis initiators caspase-8 and caspase-9, the effector caspase-3, and the apoptotic protein Bcl-2 (C) in Eca-109 cells were analyzed by western blotting. GAPDH and COX IV were used as internal protein loading controls for the cytosolic and mitochondrial fractions, respectively. (D and E) After treatment as in A, representative flow cytometric analysis of JC-1 assay was conducted, and the depolarized cells exhibited decreased red fluorescence and enhanced green fluorescence. The histogram presents the change of green fluorescence intensity in Eca-109 cells after various treatments (mean ± standard deviation, n=3, * P

    Journal: International Journal of Oncology

    Article Title: Autophagy inhibition enhances radiosensitivity of Eca-109 cells via the mitochondrial apoptosis pathway

    doi: 10.3892/ijo.2018.4349

    Figure Lengend Snippet: Involvement of mitochondrial apoptotic pathway in radiosensitivity after autophagy inhibition in Eca-109 cells. At 12 h after treatment with IR (8 Gy) with or without pretreatment with autophagy inhibitors (10 μ M for LY294002 and 1 mM for 3-MA), cytochrome c release (A) and Bax translocation to mitochondria (B), and the cellular apoptosis initiators caspase-8 and caspase-9, the effector caspase-3, and the apoptotic protein Bcl-2 (C) in Eca-109 cells were analyzed by western blotting. GAPDH and COX IV were used as internal protein loading controls for the cytosolic and mitochondrial fractions, respectively. (D and E) After treatment as in A, representative flow cytometric analysis of JC-1 assay was conducted, and the depolarized cells exhibited decreased red fluorescence and enhanced green fluorescence. The histogram presents the change of green fluorescence intensity in Eca-109 cells after various treatments (mean ± standard deviation, n=3, * P

    Article Snippet: Measurement of mitochondrial membrane potential (MMP) The MMP of Eca-109 cells was measured using the cationic dye JC-1 (Beyotime Institute of Biotechnology).

    Techniques: Inhibition, Translocation Assay, Western Blot, Flow Cytometry, Fluorescence, Standard Deviation

    Bornyl cis -4-hydroxycinnamate induced apoptosis through mitochondria potential (Δψm) change and the mitochondrial-mediated pathway in A2058 and A375 melanoma cells. ( A ) A2058 and A375 melanoma cells were treated with or without bornyl cis -4-hydroxycinnamate; Δψm in melanoma cells was detected by JC-1 staining and analyzed using fluorescence microscopy. Scale bar: 50 μm. ( B ) Changes in Bcl-2, Bcl-xl, Mcl-1, Bad, p-Bad, Bax, and cytosolic cytochrome C expression in two melanoma cells treated with different concentrations of bornyl cis -4-hydroxycinnamate visualized by western blotting analysis. β-actin was used as the internal control.

    Journal: International Journal of Molecular Sciences

    Article Title: The Effect of Bornyl cis-4-Hydroxycinnamate on Melanoma Cell Apoptosis Is Associated with Mitochondrial Dysfunction and Endoplasmic Reticulum Stress

    doi: 10.3390/ijms19051370

    Figure Lengend Snippet: Bornyl cis -4-hydroxycinnamate induced apoptosis through mitochondria potential (Δψm) change and the mitochondrial-mediated pathway in A2058 and A375 melanoma cells. ( A ) A2058 and A375 melanoma cells were treated with or without bornyl cis -4-hydroxycinnamate; Δψm in melanoma cells was detected by JC-1 staining and analyzed using fluorescence microscopy. Scale bar: 50 μm. ( B ) Changes in Bcl-2, Bcl-xl, Mcl-1, Bad, p-Bad, Bax, and cytosolic cytochrome C expression in two melanoma cells treated with different concentrations of bornyl cis -4-hydroxycinnamate visualized by western blotting analysis. β-actin was used as the internal control.

    Article Snippet: The cationic dye JC-1 was purchased from Biotium (Hayward, CA, USA).

    Techniques: Staining, Fluorescence, Microscopy, Expressing, Western Blot

    TAS restored mitochondrial transmembrane potential. HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. Mitochondrial membrane potential was determined with JC-1 staining, which was observed using a fluorescence microscope. Image of fluorescence microscope on mitochondrial membrane potential (MMP) was obtained with 200 X of magnification.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effects of Total Saponins of Aralia elata (Miq.) on Endothelial Cell Injury Induced by TNF-α via Modulation of the PI3K/Akt and NF-κB Signalling Pathways

    doi: 10.3390/ijms20010036

    Figure Lengend Snippet: TAS restored mitochondrial transmembrane potential. HUVECs were preincubated with TAS (5 μg/mL) for 2 h, followed by treatment with TNF-α (50 ng/mL) for 24 h. Mitochondrial membrane potential was determined with JC-1 staining, which was observed using a fluorescence microscope. Image of fluorescence microscope on mitochondrial membrane potential (MMP) was obtained with 200 X of magnification.

    Article Snippet: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and fluorescent dye JC-1 were acquired from Enzo Life Sciences (Farmingdale, NY, USA).

    Techniques: Staining, Fluorescence, Microscopy

    Effects of DZX on regulating the ΔYm, cleaved caspase 3 expression and caspase 3 activities in cultured cardiomyocytes. (A) Detection of ΔYm in the five groups of cardiomyocytes by fluorescent dye JC-1. Scale bar, 200 µ m. (B) Comparison of ΔYm in the five groups of cardiomyocytes. For quantification, 50 cells were randomly selected to calculate the ΔYm levels by comparing red fluorescent intensity to green fluorescent intensity. (C) Western blot images of cleaved caspase 3 expression. (D) Semi-quantitative analysis of cleaved caspase 3 expression (n=5). (E) Detection of caspase 3 activity in the five groups of cardiomyocytes. The caspase 3 activity was calculated by p-nitroaniline concentration relative to total protein concentration (n=3-6). The data are presented as mean ± standard deviation. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Opening of mitoKATP improves cardiac function and inhibits apoptosis via the AKT-Foxo1 signaling pathway in diabetic cardiomyopathy

    doi: 10.3892/ijmm.2018.3832

    Figure Lengend Snippet: Effects of DZX on regulating the ΔYm, cleaved caspase 3 expression and caspase 3 activities in cultured cardiomyocytes. (A) Detection of ΔYm in the five groups of cardiomyocytes by fluorescent dye JC-1. Scale bar, 200 µ m. (B) Comparison of ΔYm in the five groups of cardiomyocytes. For quantification, 50 cells were randomly selected to calculate the ΔYm levels by comparing red fluorescent intensity to green fluorescent intensity. (C) Western blot images of cleaved caspase 3 expression. (D) Semi-quantitative analysis of cleaved caspase 3 expression (n=5). (E) Detection of caspase 3 activity in the five groups of cardiomyocytes. The caspase 3 activity was calculated by p-nitroaniline concentration relative to total protein concentration (n=3-6). The data are presented as mean ± standard deviation. * P

    Article Snippet: Myocardial mitochondrial membrane potential (ΔYm) ΔYm was measured using fluorescent dye JC-1 (Beyotime Institute of Biotechnology).

    Techniques: Expressing, Cell Culture, Western Blot, Activity Assay, Concentration Assay, Protein Concentration, Standard Deviation

    Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by JC-1 staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p

    Journal: Scientific Reports

    Article Title: Iron stimulates plasma-activated medium-induced A549 cell injury

    doi: 10.1038/srep20928

    Figure Lengend Snippet: Mitochondrial and endoplasmic reticulum injuries by the PAM treatment. ( a ) A549 cells were treated with DMEM (vehicle); PAM in the presence or absence of catalase (50 U/mL) or BP (200 μM); FeCl 2 -supplemented DMEM (100 μM) or H 2 O 2 -supplemented DMEM (1 mM) for 2 h in a CO 2 incubator, followed by JC-1 staining. Scale bars, 50 μm. ( b , c ) A549 cells were treated for 3 h with the reagents described above in a CO 2 incubator, followed by RT-PCR for Bcl 2 ( b ), CHOP ( c ), and β-actin. RT-PCR data were normalized using β-actin levels. Data are shown as means ± SD (n = 3). * p

    Article Snippet: The Δψm was detected using the fluorescence dye JC-1 (Enzo Life Sciences, Farmingdale, NY, USA).

    Techniques: Staining, Reverse Transcription Polymerase Chain Reaction