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  • 87
    Mediatech dulbecco s modified eagle medium dmem mediatech
    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM)</t> media.
    Dulbecco S Modified Eagle Medium Dmem Mediatech, supplied by Mediatech, used in various techniques. Bioz Stars score: 87/100, based on 1545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech methods mediatech dulbecco s modified eagle s medium
    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM)</t> media.
    Methods Mediatech Dulbecco S Modified Eagle S Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s media
    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM)</t> media.
    Dulbecco S Media, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco phosphate buffered saline
    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM)</t> media.
    Dulbecco Phosphate Buffered Saline, supplied by Mediatech, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s phosphate buffered saline dpbs
    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in <t>Dulbecco’s</t> modified Eagle medium <t>(DMEM)</t> media.
    Dulbecco S Phosphate Buffered Saline Dpbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dmem
    Effect of IBTP treatment on mitochondrial respiration of <t>MB231</t> cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium <t>(DMEM,</t> containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).
    Dmem, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 4400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s pbs dpbs
    Effect of IBTP treatment on mitochondrial respiration of <t>MB231</t> cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium <t>(DMEM,</t> containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).
    Dulbecco S Pbs Dpbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 95/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s phosphate buffered saline d pbs
    Effect of IBTP treatment on mitochondrial respiration of <t>MB231</t> cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium <t>(DMEM,</t> containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).
    Dulbecco S Phosphate Buffered Saline D Pbs, supplied by Mediatech, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbeccoʼs modified eagleʼs medium
    Effect of IBTP treatment on mitochondrial respiration of <t>MB231</t> cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium <t>(DMEM,</t> containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).
    Dulbeccoʼs Modified Eagleʼs Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dmem f
    Effect of IBTP treatment on mitochondrial respiration of <t>MB231</t> cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium <t>(DMEM,</t> containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).
    Dmem F, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro dmem
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dmem, supplied by Cellgro, used in various techniques. Bioz Stars score: 99/100, based on 4087 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech iscove s modified dulbecco s complete medium
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Iscove S Modified Dulbecco S Complete Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s minimum essential medium
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dulbecco S Minimum Essential Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech iscove s modified dulbecco s medium
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Iscove S Modified Dulbecco S Medium, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro iscove modified dulbecco medium
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Iscove Modified Dulbecco Medium, supplied by Cellgro, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s phosphate buffer saline
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dulbecco S Phosphate Buffer Saline, supplied by Mediatech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dmem hg
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dmem Hg, supplied by Mediatech, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech powder dmem
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Powder Dmem, supplied by Mediatech, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbeco s modified eagle s media
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dulbeco S Modified Eagle S Media, supplied by Mediatech, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellgro dulbecco s modified eagle s medium dmem
    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh <t>DMEM</t> without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated <t>TLR4</t> as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p
    Dulbecco S Modified Eagle S Medium Dmem, supplied by Cellgro, used in various techniques. Bioz Stars score: 99/100, based on 887 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dmem f12
    NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free <t>DMEM/F12</t> medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p
    Dmem F12, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 587 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dulbecco s modification
    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
    Dulbecco S Modification, supplied by Mediatech, used in various techniques. Bioz Stars score: 99/100, based on 664 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Mediatech dmem 1x
    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in <t>Dulbecco's</t> modification of Eagle's medium containing vehicle (0.1% ethanol),
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    Image Search Results


    Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in Dulbecco’s modified Eagle medium (DMEM) media.

    Journal: Bioengineering

    Article Title: Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion

    doi: 10.3390/bioengineering5030059

    Figure Lengend Snippet: Flow testing of HMNs at 12 mL/min. ( a ) Single phase fluid blue dextran dye; and, ( b ): Two phase fluid microcapsules containing cells in Dulbecco’s modified Eagle medium (DMEM) media.

    Article Snippet: The cells were cultured in Dulbecco’s modified Eagle medium (DMEM) (Mediatech, Manassas, VA, USA) and supplemented with 10% fetal bovine serum (FBS) Life Technologies, Carlsbad, CA, USA), sodium pyruvate (Life Technologies), MEM non-essential amino acids (Life Technologies), and 1% penicillin-streptomycin (CellGro, Manassas, VA, USA).

    Techniques: Flow Cytometry, Modification

    Endocrine disrupting chemical-induction of adipocyte protein expression. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Environmental Endocrine Disruptors Promote Adipogenesis in the 3T3-L1 Cell Line through Glucocorticoid Receptor Activation

    doi: 10.1038/oby.2009.419

    Figure Lengend Snippet: Endocrine disrupting chemical-induction of adipocyte protein expression. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Article Snippet: Two days after reaching confluency, differentiation was initiated by the addition of Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Manassas, VA) containing 10% fetal bovine serum (FBS; Aleken Biologicals, Nash, TX), 167 nmol/l porcine insulin, 0–100 nmol/l dehydrocorticosterone (DHC) and 0.5 mmol/l isobutylmethylxanthine (all from Sigma, St Louis, MO).

    Techniques: Expressing, Incubation, Modification

    Dose-dependence of endocrine disrupting chemical-induced adipogenesis. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Journal: Obesity (Silver Spring, Md.)

    Article Title: Environmental Endocrine Disruptors Promote Adipogenesis in the 3T3-L1 Cell Line through Glucocorticoid Receptor Activation

    doi: 10.1038/oby.2009.419

    Figure Lengend Snippet: Dose-dependence of endocrine disrupting chemical-induced adipogenesis. 3T3-L1 differentiation was induced by incubation of confluent preadipocytes for 3 days in 10% fetal bovine serum (FBS) in Dulbecco’s modified Eagle’s medium (DMEM)

    Article Snippet: Two days after reaching confluency, differentiation was initiated by the addition of Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Manassas, VA) containing 10% fetal bovine serum (FBS; Aleken Biologicals, Nash, TX), 167 nmol/l porcine insulin, 0–100 nmol/l dehydrocorticosterone (DHC) and 0.5 mmol/l isobutylmethylxanthine (all from Sigma, St Louis, MO).

    Techniques: Incubation, Modification

    Effect of 25-hydroxycholesterol on the AMPK activator-mediated increase of PPIA normalized CYP4F2 mRNA expression in HepG2 cells. HepG2 cells were incubated with 25-hydroxycholesterol (25-OH cholesterol) (5 μg/ml) or vehicle control (DMSO, D) together with 0.5 mM AICAR (A), 100 μM genistein (G), or 75 μM resveratrol (R) for 24 h in Dulbecco's minimal essential medium containing 10% fetal bovine serum. The cells were harvested for total RNA isolation and subjected to qPCR analysis. At least four independent experiments were performed, and a representative experiment is shown. Means and standard errors were determined from triplicate samples for each treatment. Statistically significant differences between DMSO and treatments in the absence of 25-OH cholesterol (−) are indicated: **, p

    Journal: The Journal of Pharmacology and Experimental Therapeutics

    Article Title: Genistein, Resveratrol, and 5-Aminoimidazole-4-carboxamide-1-?-d-ribofuranoside Induce Cytochrome P450 4F2 Expression through an AMP-Activated Protein Kinase-Dependent Pathway S⃞

    doi: 10.1124/jpet.110.175851

    Figure Lengend Snippet: Effect of 25-hydroxycholesterol on the AMPK activator-mediated increase of PPIA normalized CYP4F2 mRNA expression in HepG2 cells. HepG2 cells were incubated with 25-hydroxycholesterol (25-OH cholesterol) (5 μg/ml) or vehicle control (DMSO, D) together with 0.5 mM AICAR (A), 100 μM genistein (G), or 75 μM resveratrol (R) for 24 h in Dulbecco's minimal essential medium containing 10% fetal bovine serum. The cells were harvested for total RNA isolation and subjected to qPCR analysis. At least four independent experiments were performed, and a representative experiment is shown. Means and standard errors were determined from triplicate samples for each treatment. Statistically significant differences between DMSO and treatments in the absence of 25-OH cholesterol (−) are indicated: **, p

    Article Snippet: The human hepatoma cell line, HepG2, was obtained from the American Type Culture Collection (Manassas, VA) and was maintained in Dulbecco's minimal essential medium (Mediatech, Herndon, VA) containing 10% fetal bovine serum (Thermo Fisher Scientific).

    Techniques: Expressing, Incubation, Isolation, Real-time Polymerase Chain Reaction

    Effect of IBTP treatment on mitochondrial respiration of MB231 cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium (DMEM, containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).

    Journal: PLoS ONE

    Article Title: A Novel Class of Mitochondria-Targeted Soft Electrophiles Modifies Mitochondrial Proteins and Inhibits Mitochondrial Metabolism in Breast Cancer Cells through Redox Mechanisms

    doi: 10.1371/journal.pone.0120460

    Figure Lengend Snippet: Effect of IBTP treatment on mitochondrial respiration of MB231 cells. Panel A: Cells plated on XF24 plates were treated with the indicated concentrations of IBTP or BTPP for 4h in 0.5% FBS-containing medium. After treatment, the medium was removed and replaced with XF assay medium (DMEM, containing 5mM glucose, 0.5% FBS, 5mM HEPES without bicarbonate) and equilibrated 1h before OCR measurement. Panel B: Cells plated on 6-well plates were treated with the indicated concentrations of IBTP or BTPP for 4h. After the incubation, the cells were harvested immediately by trypsinization. The harvested cells were replated in XF24 plates and allowed adhere for an additional 20h in complete medium containing 10% FBS (total 24h). The medium was removed and replaced with assay medium and equilibrated 1h before OCR measurement. Panel C : After 4h of IBTP or BTPP treatment, the medium was replaced with complete medium containing 10% FBS, and incubated for 48h. The cells were harvested after 48h, replated in XF24 plates and allowed adhere for an additional 20h in complete medium. The medium was replaced with assay media and incubated 1h before measurement of OCR (total duration 72h).

    Article Snippet: Breast cancer cell lines MDA-MB-231 (MB231) and MDA-MB-468 (MB468) human breast adenocarcinoma cells were cultured in DMEM (Mediatech, Manassas, VA) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Atlanta, GA).

    Techniques: XF Assay, Incubation

    BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh DMEM without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated TLR4 as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Burkholderia mallei and Burkholderia pseudomallei stimulate differential inflammatory responses from human alveolar type II cells (ATII) and macrophages

    doi: 10.3389/fcimb.2012.00165

    Figure Lengend Snippet: BM and BP stimulation of various Toll-Like Receptors. To determine if BM and/or BP stimulated various TLRs, HEK293 cells expressing TLRs 4 and 5 and transfected with the firefly luciferase plasmid pNiFty2-Luc were stimulated with BM or BP at an MOI of 100:1. Un-stimulated cells served as negative controls. The TLR agonist served as positive controls. The cells were incubated with live bacteria for 6 h. The medium was then aspirated and replaced with fresh DMEM without antibiotics and incubated overnight at 37°C and 5% CO 2 . The TLR agonists remained with the cells for an overnight incubation. The following day, samples were analyzed to determine luciferase activity. Data is expressed as relative luciferase activity. Both BM and BP (B) stimulated TLR4 as effectively as the LPS agonist (A) . However, the flagellin from BP stimulated TLR5 (B) as we detected flagellin in the medium of B-infected cells but not BM (C) . Three experiments were performed in using triplicate samples and data is represented as mean ± SEM and * denotes significant differences of p

    Article Snippet: TLR stimulation by BM or BP As described previously (Gentry et al., ), human embryonic kidney (HEK) 293 cells genetically manipulated to express human TLR4, or TLR5 (Invivogen, San Diego, CA) were cultured and maintained in DMEM (Cellgro Mediatech) supplemented with 10% FBS, 100 units/mL pencillin, 100 ug/mL streptomycin, 10 ug/mL basticidin, and 2 mM glutamine.

    Techniques: Expressing, Transfection, Luciferase, Plasmid Preparation, Incubation, Activity Assay, Infection

    NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free DMEM/F12 medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p

    Journal: Oncotarget

    Article Title: Her2 oncogene transformation enhances 5-aminolevulinic acid-mediated protoporphyrin IX production and photodynamic therapy response

    doi: 10.18632/oncotarget.11058

    Figure Lengend Snippet: NeuT oncogene transformation enhanced ALA-induced PpIX fluorescence ( A ) Fluorescence spectra of MCF10A vector and NeuT cell lysates and PpIX standard (25 ng/mL in DMSO). Both vector and NeuT cells were incubated with 1 mM ALA in serum free medium for 4 h and lysed. Fluorescence spectra of cell lysates and PpIX standard were obtained using 400 ± 2.5 nm excitation. ( B – D ) Flow cytometer analysis showing forward scatter (FSC) (B), basal cell fluorescence without ALA (C), and a dose-dependent fluorescence increase after ALA incubation (D) in MCF10A vector and NeuT cells. Cells were cultured in serum free DMEM/F12 medium with or without ALA for 4 h and cell fluorescence was measured with flow cytometry. ALA-induced fluorescence increase, calculated by subtracting basal cell fluorescence without ALA from cell fluorescence after incubation with different doses of ALA, was fit with the Michaelis-Menten enzyme kinetics. Data are presented as mean ± SD from at least 3 experiments. *** p

    Article Snippet: Cell culture and transfection MCF10A human breast epithelial cells were routinely maintained in complete DMEM/F12 (50/50) medium (Mediatech, Manassas, VA) supplemented with 5% horse serum (Atlanta Biologicals), insulin 10 ug/mL, epidermal growth factor (EGF) 20 ng/mL, cholera toxin 100 ng/mL, hydrocortisone 0.5 ug/mL and 1% of antibiotics and antimycotics solution (Mediatech) at 37° C in a humidified 5% CO2 incubator.

    Techniques: Transformation Assay, Fluorescence, Plasmid Preparation, Incubation, Flow Cytometry, Cytometry, Cell Culture

    Her2/NeuT oncogene expression transformed MCF10A human breast epithelial cells ( A ) Differential interference contrast (DIC) images (60×) show distinct differences in cell morphology between MCF10A vector control and NeuT-transformed cells. ( B ) Her2/NeuT oncogene transformation altered cell signaling. MCF10A vector and NeuT cells were cultured in complete DMEM/F12 medium, serum free medium with or without 1 mM ALA for 4 h and lysed with lysis buffer. Cell lysates were examined by Western blot for Her2/Neu signaling molecules, EMT and tight junction markers, and glycolytic enzyme PDK1.

    Journal: Oncotarget

    Article Title: Her2 oncogene transformation enhances 5-aminolevulinic acid-mediated protoporphyrin IX production and photodynamic therapy response

    doi: 10.18632/oncotarget.11058

    Figure Lengend Snippet: Her2/NeuT oncogene expression transformed MCF10A human breast epithelial cells ( A ) Differential interference contrast (DIC) images (60×) show distinct differences in cell morphology between MCF10A vector control and NeuT-transformed cells. ( B ) Her2/NeuT oncogene transformation altered cell signaling. MCF10A vector and NeuT cells were cultured in complete DMEM/F12 medium, serum free medium with or without 1 mM ALA for 4 h and lysed with lysis buffer. Cell lysates were examined by Western blot for Her2/Neu signaling molecules, EMT and tight junction markers, and glycolytic enzyme PDK1.

    Article Snippet: Cell culture and transfection MCF10A human breast epithelial cells were routinely maintained in complete DMEM/F12 (50/50) medium (Mediatech, Manassas, VA) supplemented with 5% horse serum (Atlanta Biologicals), insulin 10 ug/mL, epidermal growth factor (EGF) 20 ng/mL, cholera toxin 100 ng/mL, hydrocortisone 0.5 ug/mL and 1% of antibiotics and antimycotics solution (Mediatech) at 37° C in a humidified 5% CO2 incubator.

    Techniques: Expressing, Transformation Assay, Plasmid Preparation, Cell Culture, Lysis, Western Blot

    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in Dulbecco's modification of Eagle's medium containing vehicle (0.1% ethanol),

    Journal: Experimental and Therapeutic Medicine

    Article Title: Combination of alendronate and genistein synergistically suppresses osteoclastic differentiation of RAW267.4 cells in vitro

    doi: 10.3892/etm.2017.4695

    Figure Lengend Snippet: Combination of alendronate and genistein exhibits a synergistic suppressive effect on the proliferation of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in Dulbecco's modification of Eagle's medium containing vehicle (0.1% ethanol),

    Article Snippet: Dulbecco's Modification of Eagle's Medium (DMEM) with 4.5 g/l glucose, L-glutamine, sodium pyruvate and antibiotics [penicillin and streptomycin (P/S)] was purchased from Mediatech, Inc. (Corning, Manassas, VA, USA).

    Techniques: In Vitro, Cell Culture, Modification

    Combination of alendronate and genistein exhibits a synergistic suppressive effect on the death of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in Dulbecco's modification of Eagle's medium, and then the cells were additionally cultured

    Journal: Experimental and Therapeutic Medicine

    Article Title: Combination of alendronate and genistein synergistically suppresses osteoclastic differentiation of RAW267.4 cells in vitro

    doi: 10.3892/etm.2017.4695

    Figure Lengend Snippet: Combination of alendronate and genistein exhibits a synergistic suppressive effect on the death of RAW267.4 cells in vitro . RAW267.4 cells were cultured for 3 days in Dulbecco's modification of Eagle's medium, and then the cells were additionally cultured

    Article Snippet: Dulbecco's Modification of Eagle's Medium (DMEM) with 4.5 g/l glucose, L-glutamine, sodium pyruvate and antibiotics [penicillin and streptomycin (P/S)] was purchased from Mediatech, Inc. (Corning, Manassas, VA, USA).

    Techniques: In Vitro, Cell Culture, Modification