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  • 89
    Promega dual specific luciferase assay kit
    DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before <t>luciferase</t> assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a <t>dual-specific</t> luciferase <t>assay</t> <t>kit.</t> (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  
    Dual Specific Luciferase Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 6305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual specific luciferase assay kit/product/Promega
    Average 89 stars, based on 6305 article reviews
    Price from $9.99 to $1999.99
    dual specific luciferase assay kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    89
    Promega dual specific luciferse assay kit
    DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before <t>luciferase</t> assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a <t>dual-specific</t> luciferase <t>assay</t> <t>kit.</t> (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  
    Dual Specific Luciferse Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual specific luciferse assay kit/product/Promega
    Average 89 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    dual specific luciferse assay kit - by Bioz Stars, 2020-08
    89/100 stars
      Buy from Supplier

    99
    Promega dual luciferase reporter gene assay kit
    VEGF-A is a direct target of miR-203a-3p. ( A ) Sequence information of miR-203 binding sites in the 3′-UTR region of the VEGF-A mRNA. ( B ) <t>Dual</t> <t>luciferase</t> <t>assay</t> indicating decreased fluorescence in PMVECs co-expressing pmirGLO/VEGF-UTR and miR-203-3p mimics. ( C ) Western blotting detection of target <t>genes</t> of miR-203a-3p. Compared with mimics-NC (negative miR-203a-3p mimics control), VEGF expression decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. ( D ) qRT-PCR detection of target genes of miR-203a-3p. VEGF mRNA levels decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. Data are mean ± SEM. * P
    Dual Luciferase Reporter Gene Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase reporter gene assay kit/product/Promega
    Average 99 stars, based on 671 article reviews
    Price from $9.99 to $1999.99
    dual luciferase reporter gene assay kit - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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    DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before luciferase assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a dual-specific luciferase assay kit. (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  

    Journal: Journal of Virology

    Article Title: Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway

    doi: 10.1128/JVI.00588-19

    Figure Lengend Snippet: DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before luciferase assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a dual-specific luciferase assay kit. (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  

    Article Snippet: At 24 hpt, the cells were mock infected or infected with SeV for 12 h; the luciferase activity was then measured by using a dual-specific luciferase assay kit (Promega).

    Techniques: Inhibition, Transfection, Infection, Luciferase, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction

    FMDV VP1 protein inhibits the SeV-induced IFN-β signaling pathway. (A) The expression of VP1 inhibits SeV-induced activation of IFN-β promoters. HEK293T cells were transfected with Flag-VP1 or empty vector, together with IFN-β luciferase reporter. At 24 hpt, the cells were mock infected or infected with SeV for 12 h before luciferase assays were performed using a dual-specific luciferase assay kit. (B) VP1 negatively regulates SeV-induced activation of IFN-β at the IRF3 level. HEK293T cells were transfected with IFN-β reporter, pRL-TK, expression plasmids for Flag-VP1, and the indicated protein. Luciferase assays were performed with a dual-specific luciferase assay kit. Protein expression was analyzed by Western blotting. (C, D, and E) FMDV VP1 inhibits the phosphorylation, dimerization, and nuclear translocation of IRF3 after SeV stimulation. HEK293T cells were transfected with the indicated plasmids for 24 h. Cells were infected with SeV at various time points and then harvested for analysis by Western blotting (C) or native-PAGE (D). (E) Cells were stained with the indicated antibodies and imaged by confocal microscopy.

    Journal: Journal of Virology

    Article Title: Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway

    doi: 10.1128/JVI.00588-19

    Figure Lengend Snippet: FMDV VP1 protein inhibits the SeV-induced IFN-β signaling pathway. (A) The expression of VP1 inhibits SeV-induced activation of IFN-β promoters. HEK293T cells were transfected with Flag-VP1 or empty vector, together with IFN-β luciferase reporter. At 24 hpt, the cells were mock infected or infected with SeV for 12 h before luciferase assays were performed using a dual-specific luciferase assay kit. (B) VP1 negatively regulates SeV-induced activation of IFN-β at the IRF3 level. HEK293T cells were transfected with IFN-β reporter, pRL-TK, expression plasmids for Flag-VP1, and the indicated protein. Luciferase assays were performed with a dual-specific luciferase assay kit. Protein expression was analyzed by Western blotting. (C, D, and E) FMDV VP1 inhibits the phosphorylation, dimerization, and nuclear translocation of IRF3 after SeV stimulation. HEK293T cells were transfected with the indicated plasmids for 24 h. Cells were infected with SeV at various time points and then harvested for analysis by Western blotting (C) or native-PAGE (D). (E) Cells were stained with the indicated antibodies and imaged by confocal microscopy.

    Article Snippet: At 24 hpt, the cells were mock infected or infected with SeV for 12 h; the luciferase activity was then measured by using a dual-specific luciferase assay kit (Promega).

    Techniques: Expressing, Activation Assay, Transfection, Plasmid Preparation, Luciferase, Infection, Western Blot, Translocation Assay, Clear Native PAGE, Staining, Confocal Microscopy

    VEGF-A is a direct target of miR-203a-3p. ( A ) Sequence information of miR-203 binding sites in the 3′-UTR region of the VEGF-A mRNA. ( B ) Dual luciferase assay indicating decreased fluorescence in PMVECs co-expressing pmirGLO/VEGF-UTR and miR-203-3p mimics. ( C ) Western blotting detection of target genes of miR-203a-3p. Compared with mimics-NC (negative miR-203a-3p mimics control), VEGF expression decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. ( D ) qRT-PCR detection of target genes of miR-203a-3p. VEGF mRNA levels decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. Data are mean ± SEM. * P

    Journal: Aging (Albany NY)

    Article Title: Downregulation of lung miR-203a-3p expression by high-altitude hypoxia enhances VEGF/Notch signaling

    doi: 10.18632/aging.102878

    Figure Lengend Snippet: VEGF-A is a direct target of miR-203a-3p. ( A ) Sequence information of miR-203 binding sites in the 3′-UTR region of the VEGF-A mRNA. ( B ) Dual luciferase assay indicating decreased fluorescence in PMVECs co-expressing pmirGLO/VEGF-UTR and miR-203-3p mimics. ( C ) Western blotting detection of target genes of miR-203a-3p. Compared with mimics-NC (negative miR-203a-3p mimics control), VEGF expression decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. ( D ) qRT-PCR detection of target genes of miR-203a-3p. VEGF mRNA levels decreased after transfection with miR-203-mimics, and increased after transfection with miR-203-ASO. Data are mean ± SEM. * P

    Article Snippet: A Dual-Luciferase Reporter Gene Assay Kit (Promega, Shanghai, China) was used to assess the expression of miR-203a-3p and VEGF-A following the kit instructions.

    Techniques: Sequencing, Binding Assay, Luciferase, Fluorescence, Expressing, Western Blot, Transfection, Allele-specific Oligonucleotide, Quantitative RT-PCR