dual-luciferase reporter assay kit Search Results


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  • 99
    Millipore dual luciferase reporter assay system kit
    Dual Luciferase Reporter Assay System Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega dual luciferase reporter gene activity assay a dual luciferase reporter assay system kit
    Dual Luciferase Reporter Gene Activity Assay A Dual Luciferase Reporter Assay System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega dual luciferase reporter dlr assay kit
    miR-107 was a target of LINC00152. ( A ) The putative complementary sequences between LINC00152 and miR-107 were predicted by starBase v2.0. ( B, C ) The <t>luciferase</t> activity of LINC00152 WT or LINC00152 MUT <t>reporter</t> was detected by <t>dual-luciferase</t> reporter <t>assay.</t> ( D ) RIP assay was performed to detect the enrichment of LINC00152 by Ago2 or IgG antibody in TE-1 and KYSE30 cells transfected with miR-107 mimic or miR-NC. ( E, F ) The level of miR-107 was detected in ESCC tissues and cells using qRT-PCR. ( G ) The level of miR-107 in si-LINC00152-transfected TE-1 and KYSE30 cells was tested by qRT-PCR. ( H ) The linear correlation between the level of LINC00152 and miR-107 was shown. * P
    Dual Luciferase Reporter Dlr Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 3012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Beyotime dual luciferase reporter assay kit
    NR 4A1 regulating the transcription of adipogenesis‐related genes. A, Putative NR 4A1 binding sites (a putative NBRE ) in promoters of PPAR γ, FAS and GATA 2. B, <t>Dual‐luciferase</t> <t>reporter</t> gene <t>assay</t> for PPAR γ, FAS and GATA 2 promoters. C, Ch IP ‐ qPCR was exploited to analyse the physical association between NR 4A1 and the promoter region of PPAR γ, FAS or GATA 2. The data show the means of three independent experiments, * P
    Dual Luciferase Reporter Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega dual luciferase reporter 1000 assay kit
    NR 4A1 regulating the transcription of adipogenesis‐related genes. A, Putative NR 4A1 binding sites (a putative NBRE ) in promoters of PPAR γ, FAS and GATA 2. B, <t>Dual‐luciferase</t> <t>reporter</t> gene <t>assay</t> for PPAR γ, FAS and GATA 2 promoters. C, Ch IP ‐ qPCR was exploited to analyse the physical association between NR 4A1 and the promoter region of PPAR γ, FAS or GATA 2. The data show the means of three independent experiments, * P
    Dual Luciferase Reporter 1000 Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    BioVision dual luciferase reporter assay kits
    GAB1 is a target gene of miR-29a. A, Predicted binding site between miR-29a and GAB1. B, The binding of miR-29a to GAB1 verified by <t>dual-luciferase</t> <t>reporter</t> gene <t>assay.</t> * p
    Dual Luciferase Reporter Assay Kits, supplied by BioVision, used in various techniques. Bioz Stars score: 98/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega dual luciferase reporter gene assay kit
    Pro-apoptotic Bcl-2 antagonist killer 1 is the target <t>gene</t> for microRNA-125a A: miR-125a targeted to the 3’-UTR of the BAK1 mRNA predicted by microRNA.org. B: Results of <t>luciferase</t> <t>reporter</t> gene <t>assay;</t> *, P
    Dual Luciferase Reporter Gene Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 671 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    TransGen biotech co dual luciferase reporter assay kit
    Pro-apoptotic Bcl-2 antagonist killer 1 is the target <t>gene</t> for microRNA-125a A: miR-125a targeted to the 3’-UTR of the BAK1 mRNA predicted by microRNA.org. B: Results of <t>luciferase</t> <t>reporter</t> gene <t>assay;</t> *, P
    Dual Luciferase Reporter Assay Kit, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Beyotime dual luciferase reporter gene assay kit
    RALF1-FER-EBP1 signaling pathway regulates <t>gene</t> expression. (A, B) Venn diagram shows the overlapping numbers of up- or down-regulated genes in ebp1-1 and fer-4 mutant (A), or ebp1-1 and RALF1-treated Col-0 (B), comparing with Col-0 without RALF1 treatment. (C) Venn diagram shows the total and overlapping numbers of EBP1-, FER-, and RALF1-regulated genes. (D) CML38 promoter was immunoprecipitated by EBP1 via ChIP <t>assay.</t> ChIP-qPCR results were quantified by normalization of the EBP1-IP signal with the corresponding Input signal (IP/Input). RALF1 treatment (1 μM) was performed for 2 hours on 7-DAG Col-0. Preimmune serum (“Preim”) was used for negative control. Data shown are representative of three independent experiments with similar results. (E) Competitive EMSA shows interaction of EBP1 with the FITC-labeled “CCACGTC” DNA directly. Unlabeled “CCACGTC” motif was used as the competitor. (F) <t>Dual-LUC</t> shows relative <t>reporter</t> activity (LUC/REN) of indicated genotypes (Col-0 or fer-4 ). RALF1 treatment (0.1 μM RALF1) condition, proCML38 ::LUC (“ CML38 ”), and EBP1 protein expression are shown. Quantification of LUC relative to REN levels (“LUC/REN”) was performed on three replicates. Data shown in (E) and (F) are representative of four independent experiments with similar results. Data points show means +/− SD. Values with different letters are significantly different ( P . ChIP, chromatin immunoprecipitation; DAG, day after gemination; Dual-LUC, transient transcription <t>dual-luciferase</t> assay; EBP1, ErbB3-binding protein 1; EMSA, electrophoretic mobility shift assay; FER, FERONIA; FITC, fluorescein isothiocyanate; GST, glutathione S-transferase; LUC, luciferase; qPCR, quantitative PCR; RALF1, rapid alkalinization factor 1; REN, Renilla luciferase; RIPK, RPM1-induced protein kinase.
    Dual Luciferase Reporter Gene Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 91/100, based on 564 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher dual luciferase reporter assay kit
    GRP78 is a target of miR-203a-3p. (A) The predicted binding sites of miR-203a-3p in the 3′-UTR of GRP78 mRNA. Expressions of (B) miR-203a-3p and (C) GRP78 mRNA were detected by RT-PCR analysis. (D) U6 and GAPDH were used as the internal control, respectively. n=8. <t>Dual-luciferase</t> <t>reporter</t> <t>assay</t> was performed to detect the interaction between miR-203a-3p and the 3′-UTR of GRP78. n=8. (E and F) miR-203a-3p inhibited the protein expression of GRP78, which was detected by western blot analysis, in infected with miR-203a-3p mimics or miR-203a-3p inhibitor (anti-miR-203a-3p). GAPDH was used as the internal control. n=3. Δ P
    Dual Luciferase Reporter Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 117 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vazyme Biotech Co dual luciferase reporter assay kit
    HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. <t>Luciferase</t> activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via <t>dual-luciferase</t> <t>reporter</t> <t>assay.</t> (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. * P
    Dual Luciferase Reporter Assay Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Beijing Solarbio Science dual luciferase reporter assay kit
    HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. <t>Luciferase</t> activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via <t>dual-luciferase</t> <t>reporter</t> <t>assay.</t> (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. * P
    Dual Luciferase Reporter Assay Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega dual glo luciferase reporter assay kit
    HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. <t>Luciferase</t> activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via <t>dual-luciferase</t> <t>reporter</t> <t>assay.</t> (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. * P
    Dual Glo Luciferase Reporter Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 218 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    miR-107 was a target of LINC00152. ( A ) The putative complementary sequences between LINC00152 and miR-107 were predicted by starBase v2.0. ( B, C ) The luciferase activity of LINC00152 WT or LINC00152 MUT reporter was detected by dual-luciferase reporter assay. ( D ) RIP assay was performed to detect the enrichment of LINC00152 by Ago2 or IgG antibody in TE-1 and KYSE30 cells transfected with miR-107 mimic or miR-NC. ( E, F ) The level of miR-107 was detected in ESCC tissues and cells using qRT-PCR. ( G ) The level of miR-107 in si-LINC00152-transfected TE-1 and KYSE30 cells was tested by qRT-PCR. ( H ) The linear correlation between the level of LINC00152 and miR-107 was shown. * P

    Journal: OncoTargets and therapy

    Article Title: Long Non-Coding RNA LINC00152 Regulates Cell Proliferation, Migration And Invasion In Esophageal Squamous Cell Carcinoma Via miR-107/Rab10 Axis

    doi: 10.2147/OTT.S221515

    Figure Lengend Snippet: miR-107 was a target of LINC00152. ( A ) The putative complementary sequences between LINC00152 and miR-107 were predicted by starBase v2.0. ( B, C ) The luciferase activity of LINC00152 WT or LINC00152 MUT reporter was detected by dual-luciferase reporter assay. ( D ) RIP assay was performed to detect the enrichment of LINC00152 by Ago2 or IgG antibody in TE-1 and KYSE30 cells transfected with miR-107 mimic or miR-NC. ( E, F ) The level of miR-107 was detected in ESCC tissues and cells using qRT-PCR. ( G ) The level of miR-107 in si-LINC00152-transfected TE-1 and KYSE30 cells was tested by qRT-PCR. ( H ) The linear correlation between the level of LINC00152 and miR-107 was shown. * P

    Article Snippet: The luciferase reporter activity was measured using dual-luciferase reporter assay kit (Promega).

    Techniques: Luciferase, Activity Assay, Reporter Assay, Transfection, Quantitative RT-PCR

    Rab10 directly interacted with miR-107 in ESCC. ( A ) The putative binding sites between miR-107 and Rab10 3ʹUTR were shown, as well as the mutant sequences. ( B, C ) The luciferase activity of Rab10 3ʹUTR-WT or Rab10 3ʹUTR-MUT reporter was detected by dual-luciferase reporter assay. ( D – F ) The mRNA and protein levels of Rab10 in ESCC tissues and cells were tested by qRT-PCR and Western blot assay. ( G, H ) The protein level of Rab10 in TE-1 and KYSE30 cells transfected with miR-107 (miR-NC) or si-LINC00152 (si-NC) was assessed by Western blot assay. ( I and J ) The correlation among the expression of Rab10, miR-107 and LINC00152 was shown. * P

    Journal: OncoTargets and therapy

    Article Title: Long Non-Coding RNA LINC00152 Regulates Cell Proliferation, Migration And Invasion In Esophageal Squamous Cell Carcinoma Via miR-107/Rab10 Axis

    doi: 10.2147/OTT.S221515

    Figure Lengend Snippet: Rab10 directly interacted with miR-107 in ESCC. ( A ) The putative binding sites between miR-107 and Rab10 3ʹUTR were shown, as well as the mutant sequences. ( B, C ) The luciferase activity of Rab10 3ʹUTR-WT or Rab10 3ʹUTR-MUT reporter was detected by dual-luciferase reporter assay. ( D – F ) The mRNA and protein levels of Rab10 in ESCC tissues and cells were tested by qRT-PCR and Western blot assay. ( G, H ) The protein level of Rab10 in TE-1 and KYSE30 cells transfected with miR-107 (miR-NC) or si-LINC00152 (si-NC) was assessed by Western blot assay. ( I and J ) The correlation among the expression of Rab10, miR-107 and LINC00152 was shown. * P

    Article Snippet: The luciferase reporter activity was measured using dual-luciferase reporter assay kit (Promega).

    Techniques: Binding Assay, Mutagenesis, Luciferase, Activity Assay, Reporter Assay, Quantitative RT-PCR, Western Blot, Transfection, Expressing

    Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced activator protein-1 (AP-1) activation in MCF-7 cells. (A) Phosphorylation of c-Jun, a major subunit of AP-1, was determined by western blots with proliferating cell nuclear antigen (PCNA) as the loading control for nuclear proteins. (B) AP-1-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and AP-1 promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. AP-1 DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Journal: Journal of Breast Cancer

    Article Title: Troglitazone Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Breast Cancer Cell through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism

    doi: 10.4048/jbc.2018.21.1.28

    Figure Lengend Snippet: Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced activator protein-1 (AP-1) activation in MCF-7 cells. (A) Phosphorylation of c-Jun, a major subunit of AP-1, was determined by western blots with proliferating cell nuclear antigen (PCNA) as the loading control for nuclear proteins. (B) AP-1-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and AP-1 promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. AP-1 DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Article Snippet: Dual Luciferase Reporter Assay Kits (Promega) and Lumat LB 9507 luminometer (EG & G Berthold, Gaithersburg, USA) were used to detect luciferase activity.

    Techniques: Activation Assay, Western Blot, Luciferase, Plasmid Preparation, Transfection, Activity Assay, Binding Assay, Electrophoretic Mobility Shift Assay

    Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Journal: Journal of Breast Cancer

    Article Title: Troglitazone Inhibits Matrix Metalloproteinase-9 Expression and Invasion of Breast Cancer Cell through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism

    doi: 10.4048/jbc.2018.21.1.28

    Figure Lengend Snippet: Troglitazone inhibits 12- O -tetradecanoylphorbol-13-acetate (TPA)-induced nuclear factor κB (NF-κB) activation in MCF-7 cells. (A) Cells were treated with troglitazone with TPA and nuclear extracts were prepared after 3 hours. Translocation of p65 and p50 into the nucleus and phosphorylation of IκB kinase (IKK) α/β and nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor (IκB) α in cytosol was determined by western blots. Proliferating cell nuclear antigen (PCNA) was the loading control for nuclear proteins. (B) NF-κB-luc reporters and a Renilla luciferase thymidine kinase reporter vector were co-transfected into MCF-7 cells. Cells were treated with troglitazone with TPA and NF-κB promoter activity was measured with dual-luciferase reporter assays. (C) Cells were treated with troglitazone with TPA and nuclear extracts made after 3 hours. NF-κB DNA binding was analyzed by electrophoretic mobility shift assay. Values are mean±standard error of the mean of three independent experiments. * p

    Article Snippet: Dual Luciferase Reporter Assay Kits (Promega) and Lumat LB 9507 luminometer (EG & G Berthold, Gaithersburg, USA) were used to detect luciferase activity.

    Techniques: Activation Assay, Translocation Assay, Western Blot, Luciferase, Plasmid Preparation, Transfection, Activity Assay, Binding Assay, Electrophoretic Mobility Shift Assay

    MiR21 induced ICOS expression on Treg cells through p-STAT3 upregulation. a Treg cells were sorted from the co-culture system for further study, ICOS expression on Treg cells was increased by miR21 overexpression in the co-culture systems of SU-DHL-4 and SU-DHL-8 cells . b The effect of miR21 on transcriptional activity of the PDCD4 promoter was measured by luciferase reporter assay in HEK-293 T cells transfected with control mimics or miR21 mimics. c Downregulation of PDCD4 and upregulation of p-STAT3 were detected in Treg cells. d Increased ICOS expression on Treg cells was blocked by the STAT inhibitor (Representative immunofluorescene images of ICOS ( green )/ICOSL ( red ) with cells counterstained with DAPI ( blue ))

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: MiR21 sensitized B-lymphoma cells to ABT-199 via ICOS/ICOSL-mediated interaction of Treg cells with endothelial cells

    doi: 10.1186/s13046-017-0551-z

    Figure Lengend Snippet: MiR21 induced ICOS expression on Treg cells through p-STAT3 upregulation. a Treg cells were sorted from the co-culture system for further study, ICOS expression on Treg cells was increased by miR21 overexpression in the co-culture systems of SU-DHL-4 and SU-DHL-8 cells . b The effect of miR21 on transcriptional activity of the PDCD4 promoter was measured by luciferase reporter assay in HEK-293 T cells transfected with control mimics or miR21 mimics. c Downregulation of PDCD4 and upregulation of p-STAT3 were detected in Treg cells. d Increased ICOS expression on Treg cells was blocked by the STAT inhibitor (Representative immunofluorescene images of ICOS ( green )/ICOSL ( red ) with cells counterstained with DAPI ( blue ))

    Article Snippet: Protein was collected 24 h after transfection, using the Passive Lysis Buffer (30 μL per well) provided as part of the Dual-Luciferase Reporter Assay System kit (Promega).

    Techniques: Expressing, Co-Culture Assay, Over Expression, Activity Assay, Luciferase, Reporter Assay, Transfection

    NR 4A1 regulating the transcription of adipogenesis‐related genes. A, Putative NR 4A1 binding sites (a putative NBRE ) in promoters of PPAR γ, FAS and GATA 2. B, Dual‐luciferase reporter gene assay for PPAR γ, FAS and GATA 2 promoters. C, Ch IP ‐ qPCR was exploited to analyse the physical association between NR 4A1 and the promoter region of PPAR γ, FAS or GATA 2. The data show the means of three independent experiments, * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression, et al. NR4A1 retards adipocyte differentiation or maturation via enhancing GATA2 and p53 expression

    doi: 10.1111/jcmm.13715

    Figure Lengend Snippet: NR 4A1 regulating the transcription of adipogenesis‐related genes. A, Putative NR 4A1 binding sites (a putative NBRE ) in promoters of PPAR γ, FAS and GATA 2. B, Dual‐luciferase reporter gene assay for PPAR γ, FAS and GATA 2 promoters. C, Ch IP ‐ qPCR was exploited to analyse the physical association between NR 4A1 and the promoter region of PPAR γ, FAS or GATA 2. The data show the means of three independent experiments, * P

    Article Snippet: At 24 hours post‐transfection, the luciferase activity in each well was measured with a dual‐luciferase reporter assay kit (Beyotime Institute of Biotechnology, Shanghai, China) according to the manufacturer's instructions.

    Techniques: Binding Assay, Luciferase, Reporter Gene Assay, Real-time Polymerase Chain Reaction

    AML cells transfer miR-4532 by exosomes to target LDOC1. a Venn diagram of the predicted target genes of miR-4532. b The targeting relationship between miR-4532 and LDOC1 in HEK-293 T cells and CD34 + HSCs verified by dual-luciferase reporter gene assay (* p

    Journal: Stem Cell Research & Therapy

    Article Title: Acute myeloid leukemia cells secrete microRNA-4532-containing exosomes to mediate normal hematopoiesis in hematopoietic stem cells by activating the LDOC1-dependent STAT3 signaling pathway

    doi: 10.1186/s13287-019-1475-7

    Figure Lengend Snippet: AML cells transfer miR-4532 by exosomes to target LDOC1. a Venn diagram of the predicted target genes of miR-4532. b The targeting relationship between miR-4532 and LDOC1 in HEK-293 T cells and CD34 + HSCs verified by dual-luciferase reporter gene assay (* p

    Article Snippet: After 48 h, the cells were lysed and the luciferase activity was determined using Dual-Luciferase Reporter Assay kits (RG005, Beyotime Biotechnology, Shanghai, China) in the Glomax20/20 luminometer fluorescence detector (Promega, Madison, WI, USA).

    Techniques: Luciferase, Reporter Gene Assay

    GAB1 is a target gene of miR-29a. A, Predicted binding site between miR-29a and GAB1. B, The binding of miR-29a to GAB1 verified by dual-luciferase reporter gene assay. * p

    Journal: Molecular Medicine

    Article Title: Inhibition of microRNA-29a alleviates hyperoxia-induced bronchopulmonary dysplasia in neonatal mice via upregulation of GAB1

    doi: 10.1186/s10020-019-0127-9

    Figure Lengend Snippet: GAB1 is a target gene of miR-29a. A, Predicted binding site between miR-29a and GAB1. B, The binding of miR-29a to GAB1 verified by dual-luciferase reporter gene assay. * p

    Article Snippet: Dual-Luciferase Reporter assay kit (K801–200, Biovision, Milpitas, CA, USA) and Glomax 20/20 luminometer fluorescence detector (Promega, Madison, WI, USA) were applied for luciferase activity detection.

    Techniques: Binding Assay, Luciferase, Reporter Gene Assay

    The identification of competitive binding of HOTAIR to miR‐130a as well as bindings of miR‐130a and IGF1. Note: A, the binding sites of HOTAIR to miR‐130a predicted by bioinformatic online software; B, the luciferase activity detected by dual‐luciferase reporter gene assay to verify the binding of HOTAIR to miR‐130a; C, the binding of HOTAIR to miR‐130a confirmed by RNA‐pull down assay; D, the expression of miR‐130a in the ovarian granulosa cells; E, the mRNA expression of IGF1 in the ovarian granulosa cells; F, the protein expression of IGF1 in the ovarian granulosa cells; G, the protein band patterns of IGF1 in the ovarian granulosa cells; H, the binding sites of miR‐130a to IGF1 predicted by bioinformatic online software; I, the luciferase activity detected by dual‐luciferase reporter gene assay to verify the targeting relationship of miR‐130a to IGF1; * P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a. Down‐regulated lncRNA HOTAIR alleviates polycystic ovaries syndrome in rats by reducing expression of insulin‐like growth factor 1 via microRNA‐130a

    doi: 10.1111/jcmm.14753

    Figure Lengend Snippet: The identification of competitive binding of HOTAIR to miR‐130a as well as bindings of miR‐130a and IGF1. Note: A, the binding sites of HOTAIR to miR‐130a predicted by bioinformatic online software; B, the luciferase activity detected by dual‐luciferase reporter gene assay to verify the binding of HOTAIR to miR‐130a; C, the binding of HOTAIR to miR‐130a confirmed by RNA‐pull down assay; D, the expression of miR‐130a in the ovarian granulosa cells; E, the mRNA expression of IGF1 in the ovarian granulosa cells; F, the protein expression of IGF1 in the ovarian granulosa cells; G, the protein band patterns of IGF1 in the ovarian granulosa cells; H, the binding sites of miR‐130a to IGF1 predicted by bioinformatic online software; I, the luciferase activity detected by dual‐luciferase reporter gene assay to verify the targeting relationship of miR‐130a to IGF1; * P

    Article Snippet: The luciferase activity was evaluated by a dual‐luciferase reporter gene assay kit (Biovision) and a Glomax20/20 luminometer (Promega, Madison, WI, USA).

    Techniques: Binding Assay, Software, Luciferase, Activity Assay, Reporter Gene Assay, Pull Down Assay, Expressing

    Pro-apoptotic Bcl-2 antagonist killer 1 is the target gene for microRNA-125a A: miR-125a targeted to the 3’-UTR of the BAK1 mRNA predicted by microRNA.org. B: Results of luciferase reporter gene assay; *, P

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1

    doi: 10.22038/IJBMS.2017.9542

    Figure Lengend Snippet: Pro-apoptotic Bcl-2 antagonist killer 1 is the target gene for microRNA-125a A: miR-125a targeted to the 3’-UTR of the BAK1 mRNA predicted by microRNA.org. B: Results of luciferase reporter gene assay; *, P

    Article Snippet: NPCs in the logarithmic growth phase were obtained and inocul- ated onto 24-well plate, followed by Lipofectamine 2000 (Invitrogen Inc., Carlsbad, CA, USA) transfection, and the detection of luciferase activity by Dual Luciferase Reporter Gene Assay Kit (Promega Corporation, Madison, WI, USA).

    Techniques: Luciferase, Reporter Gene Assay

    RALF1-FER-EBP1 signaling pathway regulates gene expression. (A, B) Venn diagram shows the overlapping numbers of up- or down-regulated genes in ebp1-1 and fer-4 mutant (A), or ebp1-1 and RALF1-treated Col-0 (B), comparing with Col-0 without RALF1 treatment. (C) Venn diagram shows the total and overlapping numbers of EBP1-, FER-, and RALF1-regulated genes. (D) CML38 promoter was immunoprecipitated by EBP1 via ChIP assay. ChIP-qPCR results were quantified by normalization of the EBP1-IP signal with the corresponding Input signal (IP/Input). RALF1 treatment (1 μM) was performed for 2 hours on 7-DAG Col-0. Preimmune serum (“Preim”) was used for negative control. Data shown are representative of three independent experiments with similar results. (E) Competitive EMSA shows interaction of EBP1 with the FITC-labeled “CCACGTC” DNA directly. Unlabeled “CCACGTC” motif was used as the competitor. (F) Dual-LUC shows relative reporter activity (LUC/REN) of indicated genotypes (Col-0 or fer-4 ). RALF1 treatment (0.1 μM RALF1) condition, proCML38 ::LUC (“ CML38 ”), and EBP1 protein expression are shown. Quantification of LUC relative to REN levels (“LUC/REN”) was performed on three replicates. Data shown in (E) and (F) are representative of four independent experiments with similar results. Data points show means +/− SD. Values with different letters are significantly different ( P . ChIP, chromatin immunoprecipitation; DAG, day after gemination; Dual-LUC, transient transcription dual-luciferase assay; EBP1, ErbB3-binding protein 1; EMSA, electrophoretic mobility shift assay; FER, FERONIA; FITC, fluorescein isothiocyanate; GST, glutathione S-transferase; LUC, luciferase; qPCR, quantitative PCR; RALF1, rapid alkalinization factor 1; REN, Renilla luciferase; RIPK, RPM1-induced protein kinase.

    Journal: PLoS Biology

    Article Title: EBP1 nuclear accumulation negatively feeds back on FERONIA-mediated RALF1 signaling

    doi: 10.1371/journal.pbio.2006340

    Figure Lengend Snippet: RALF1-FER-EBP1 signaling pathway regulates gene expression. (A, B) Venn diagram shows the overlapping numbers of up- or down-regulated genes in ebp1-1 and fer-4 mutant (A), or ebp1-1 and RALF1-treated Col-0 (B), comparing with Col-0 without RALF1 treatment. (C) Venn diagram shows the total and overlapping numbers of EBP1-, FER-, and RALF1-regulated genes. (D) CML38 promoter was immunoprecipitated by EBP1 via ChIP assay. ChIP-qPCR results were quantified by normalization of the EBP1-IP signal with the corresponding Input signal (IP/Input). RALF1 treatment (1 μM) was performed for 2 hours on 7-DAG Col-0. Preimmune serum (“Preim”) was used for negative control. Data shown are representative of three independent experiments with similar results. (E) Competitive EMSA shows interaction of EBP1 with the FITC-labeled “CCACGTC” DNA directly. Unlabeled “CCACGTC” motif was used as the competitor. (F) Dual-LUC shows relative reporter activity (LUC/REN) of indicated genotypes (Col-0 or fer-4 ). RALF1 treatment (0.1 μM RALF1) condition, proCML38 ::LUC (“ CML38 ”), and EBP1 protein expression are shown. Quantification of LUC relative to REN levels (“LUC/REN”) was performed on three replicates. Data shown in (E) and (F) are representative of four independent experiments with similar results. Data points show means +/− SD. Values with different letters are significantly different ( P . ChIP, chromatin immunoprecipitation; DAG, day after gemination; Dual-LUC, transient transcription dual-luciferase assay; EBP1, ErbB3-binding protein 1; EMSA, electrophoretic mobility shift assay; FER, FERONIA; FITC, fluorescein isothiocyanate; GST, glutathione S-transferase; LUC, luciferase; qPCR, quantitative PCR; RALF1, rapid alkalinization factor 1; REN, Renilla luciferase; RIPK, RPM1-induced protein kinase.

    Article Snippet: Samples were collected for the Dual-LUC using Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime).

    Techniques: Expressing, Mutagenesis, Immunoprecipitation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction, Negative Control, Labeling, Activity Assay, Luciferase, Binding Assay, Electrophoretic Mobility Shift Assay

    GRP78 is a target of miR-203a-3p. (A) The predicted binding sites of miR-203a-3p in the 3′-UTR of GRP78 mRNA. Expressions of (B) miR-203a-3p and (C) GRP78 mRNA were detected by RT-PCR analysis. (D) U6 and GAPDH were used as the internal control, respectively. n=8. Dual-luciferase reporter assay was performed to detect the interaction between miR-203a-3p and the 3′-UTR of GRP78. n=8. (E and F) miR-203a-3p inhibited the protein expression of GRP78, which was detected by western blot analysis, in infected with miR-203a-3p mimics or miR-203a-3p inhibitor (anti-miR-203a-3p). GAPDH was used as the internal control. n=3. Δ P

    Journal: Molecular Medicine Reports

    Article Title: Mechanism of Astragalus polysaccharides in attenuating insulin resistance in Rats with type 2 diabetes mellitus via the regulation of liver microRNA-203a-3p

    doi: 10.3892/mmr.2017.8084

    Figure Lengend Snippet: GRP78 is a target of miR-203a-3p. (A) The predicted binding sites of miR-203a-3p in the 3′-UTR of GRP78 mRNA. Expressions of (B) miR-203a-3p and (C) GRP78 mRNA were detected by RT-PCR analysis. (D) U6 and GAPDH were used as the internal control, respectively. n=8. Dual-luciferase reporter assay was performed to detect the interaction between miR-203a-3p and the 3′-UTR of GRP78. n=8. (E and F) miR-203a-3p inhibited the protein expression of GRP78, which was detected by western blot analysis, in infected with miR-203a-3p mimics or miR-203a-3p inhibitor (anti-miR-203a-3p). GAPDH was used as the internal control. n=3. Δ P

    Article Snippet: The cells were harvested 24 h after transfection, and luciferase activity was measured with a dual luciferase reporter assay kit (Thermo Fisher Scientific, Inc.) on a luminometer (Lumat LB9507), as we described previously ( ).

    Techniques: Binding Assay, Reverse Transcription Polymerase Chain Reaction, Luciferase, Reporter Assay, Expressing, Western Blot, Infection

    HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. Luciferase activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via dual-luciferase reporter assay. (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. * P

    Journal: International Journal of Molecular Medicine

    Article Title: Propofol-induced HOXA11-AS promotes proliferation, migration and invasion, but inhibits apoptosis in hepatocellular carcinoma cells by targeting miR-4458

    doi: 10.3892/ijmm.2020.4667

    Figure Lengend Snippet: HOXA11-AS negatively regulates miR-4458 expression via direct targeting in HCC cells. (A) Putative binding sites between HOXA11-AS and miR-4458 were predicted using starBase 2.0. Luciferase activity in HOXA11-AS WT or HOXA11-AS MUT and miR-4458 or miR-NC co-transfected (B) Hep3B and (C) Huh-7 cells was detected via dual-luciferase reporter assay. (D) RNA pull-down assay was conducted to assess the interaction between HOXA11-AS and miR-4458, and the expression of HOXA11-AS was detected using RT-qPCR after Bio-miR-4458 or Bio-NC was transfected into Hep3B and Huh-7 cells. (E) Expression of HOXA11-AS in Hep3B and Huh-7 cells transfected with si-NC, si-HOXA11-AS#1, si-HOXA11-AS#2 or si-HOXA11-AS#3 was determined via RT-qPCR. (F) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with Vector or HOXA11-AS was determined via RT-qPCR. (G) Expression of miR-4458 in Hep3B and Huh-7 cells transfected with si-NC or si-HOXA11-AS#1 was measured using RT-qPCR. Experiments were repeated three times. * P

    Article Snippet: After co-transfection for 48 h, a dual-luciferase reporter assay kit (cat. no. DL101-01; Vazyme Biotech Co., Ltd.) was used to determine luciferase activity.

    Techniques: Expressing, Binding Assay, Luciferase, Activity Assay, Transfection, Reporter Assay, Pull Down Assay, Quantitative RT-PCR, Plasmid Preparation