dual luciferase assay kit Search Results


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  • 90
    Thermo Fisher dual luciferase kit
    Dual Luciferase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase kit/product/Thermo Fisher
    Average 90 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    dual luciferase kit - by Bioz Stars, 2020-08
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    94
    Promega dual luciferase kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 9262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase kit/product/Promega
    Average 94 stars, based on 9262 article reviews
    Price from $9.99 to $1999.99
    dual luciferase kit - by Bioz Stars, 2020-08
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    93
    Beyotime dual luciferase assay kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 93/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase assay kit/product/Beyotime
    Average 93 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    dual luciferase assay kit - by Bioz Stars, 2020-08
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    92
    Genecopoeia dual luciferase assay kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Assay Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 92/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase assay kit/product/Genecopoeia
    Average 92 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    dual luciferase assay kit - by Bioz Stars, 2020-08
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    85
    Promega dual luciferase kit protocol
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Kit Protocol, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase kit protocol/product/Promega
    Average 85 stars, based on 10 article reviews
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    dual luciferase kit protocol - by Bioz Stars, 2020-08
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    88
    Promega dual glow luciferase kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Glow Luciferase Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual glow luciferase kit/product/Promega
    Average 88 stars, based on 62 article reviews
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    dual glow luciferase kit - by Bioz Stars, 2020-08
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    88
    Promega dual luciferase reagent kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase reagent kit/product/Promega
    Average 88 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    dual luciferase reagent kit - by Bioz Stars, 2020-08
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    92
    Stratagene dual luciferase assay kit
    A <t>dual</t> <t>luciferase</t> reporter <t>assay</t> validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001
    Dual Luciferase Assay Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dual luciferase assay kit/product/Stratagene
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    dual luciferase assay kit - by Bioz Stars, 2020-08
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    Image Search Results


    A dual luciferase reporter assay validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001

    Journal: Frontiers in Plant Science

    Article Title: EjTFL1 Genes Promote Growth but Inhibit Flower Bud Differentiation in Loquat

    doi: 10.3389/fpls.2020.00576

    Figure Lengend Snippet: A dual luciferase reporter assay validated the transcriptional activation of EjAP1 promoters by EjTFL1s, EjFD, and EjTFL1s-EjFD. (A) Schematic diagram of effector and reporter plasmid construction. (B) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-1 promoter. (C) EjTFL1s, EjFD, and EjTFL1s-EjFD act on the EjAP1-2 promoter. The activation capabilities of EjTFL1s and EjFD were calculated by the ratio of LUC to REN. The ratio of empty vector LUC/REN was used as a control and set as 1. Error bars represent ± SE from three biological replicates. Asterisks indicate significantly different values compared with those for the empty vector (calculated by Student’s t -test), 0.0001

    Article Snippet: After 48–72 h, LUC and REN luciferases were quantified using the Dual Luciferase Assay kit (Promega, United States) with a Luminoskan Ascent Microplate Luminometer (Thermo, United States).

    Techniques: Luciferase, Reporter Assay, Activation Assay, Plasmid Preparation

    DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before luciferase assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a dual-specific luciferase assay kit. (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  

    Journal: Journal of Virology

    Article Title: Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway

    doi: 10.1128/JVI.00588-19

    Figure Lengend Snippet: DNAJA3 restores VP1-induced inhibitory effect on IFN-β signaling pathway. (A) DNAJA3 restores the inhibition of VP1 on IFN-β signaling pathway in a dose-dependent manner. The HEK293T cells were transfected indicated plasmids and then mock infected or infected with SeV for 12 h before luciferase assays were performed. (B and C) VP1 inhibits the IFN-β or its downstream ISGs more efficiently on DNAJA3-KO PK-15 cells than on WT cells. DNAJA3-WT and DNAJA3-KO PK-15 cells were transfected with Flag-VP1 or vector. At 24 hpt, the cells were transfected by Lipofectamine 2000 with or without poly(I⋠C) at 50 ng/ml for 12 h. (B) Luciferase assays were performed using a dual-specific luciferase assay kit. (C) The IFN-β, ISG15, MX1, ISG54, and GBP1 mRNA levels were detected by RT-PCR. **, P  

    Article Snippet: At 24 hpt, the cells were mock infected or infected with SeV for 12 h; the luciferase activity was then measured by using a dual-specific luciferase assay kit (Promega).

    Techniques: Inhibition, Transfection, Infection, Luciferase, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction

    FMDV VP1 protein inhibits the SeV-induced IFN-β signaling pathway. (A) The expression of VP1 inhibits SeV-induced activation of IFN-β promoters. HEK293T cells were transfected with Flag-VP1 or empty vector, together with IFN-β luciferase reporter. At 24 hpt, the cells were mock infected or infected with SeV for 12 h before luciferase assays were performed using a dual-specific luciferase assay kit. (B) VP1 negatively regulates SeV-induced activation of IFN-β at the IRF3 level. HEK293T cells were transfected with IFN-β reporter, pRL-TK, expression plasmids for Flag-VP1, and the indicated protein. Luciferase assays were performed with a dual-specific luciferase assay kit. Protein expression was analyzed by Western blotting. (C, D, and E) FMDV VP1 inhibits the phosphorylation, dimerization, and nuclear translocation of IRF3 after SeV stimulation. HEK293T cells were transfected with the indicated plasmids for 24 h. Cells were infected with SeV at various time points and then harvested for analysis by Western blotting (C) or native-PAGE (D). (E) Cells were stained with the indicated antibodies and imaged by confocal microscopy.

    Journal: Journal of Virology

    Article Title: Cellular DNAJA3, a Novel VP1-Interacting Protein, Inhibits Foot-and-Mouth Disease Virus Replication by Inducing Lysosomal Degradation of VP1 and Attenuating Its Antagonistic Role in the Beta Interferon Signaling Pathway

    doi: 10.1128/JVI.00588-19

    Figure Lengend Snippet: FMDV VP1 protein inhibits the SeV-induced IFN-β signaling pathway. (A) The expression of VP1 inhibits SeV-induced activation of IFN-β promoters. HEK293T cells were transfected with Flag-VP1 or empty vector, together with IFN-β luciferase reporter. At 24 hpt, the cells were mock infected or infected with SeV for 12 h before luciferase assays were performed using a dual-specific luciferase assay kit. (B) VP1 negatively regulates SeV-induced activation of IFN-β at the IRF3 level. HEK293T cells were transfected with IFN-β reporter, pRL-TK, expression plasmids for Flag-VP1, and the indicated protein. Luciferase assays were performed with a dual-specific luciferase assay kit. Protein expression was analyzed by Western blotting. (C, D, and E) FMDV VP1 inhibits the phosphorylation, dimerization, and nuclear translocation of IRF3 after SeV stimulation. HEK293T cells were transfected with the indicated plasmids for 24 h. Cells were infected with SeV at various time points and then harvested for analysis by Western blotting (C) or native-PAGE (D). (E) Cells were stained with the indicated antibodies and imaged by confocal microscopy.

    Article Snippet: At 24 hpt, the cells were mock infected or infected with SeV for 12 h; the luciferase activity was then measured by using a dual-specific luciferase assay kit (Promega).

    Techniques: Expressing, Activation Assay, Transfection, Plasmid Preparation, Luciferase, Infection, Western Blot, Translocation Assay, Clear Native PAGE, Staining, Confocal Microscopy