Journal: Scientific Reports
Article Title: Copper(I)-binding properties of de-coppering drugs for the treatment of Wilson disease. α-Lipoic acid as a potential anti-copper agent
Figure Lengend Snippet: Determination of the relative Cu(I)-binding affinity of de-coppering drugs in competition with Cu 1 Cox17. Fractional occupancy of Cu(I)-binding sites in Cox17 at different concentrations of PA ( a ), TR ( b ), BAL ( c ), DMS ( d ) and TTM ( e ) in a metal competition experiment. Conditions: Cox17 1 μM; 20 mM ammonium acetate, pH = 7.3, DTT 50 μM; T = 25 °C. Results of duplicate experiments are presented with different symbols. The solid line shows the fitting curve with hyperbolic equation (y = P1*(1 − [x/(P2 + x)]) + P3), where P2 = C 50 .
Article Snippet: Reagents Chemical reagents: PA, TR, BAL, DMS, LA, ammonium TTM, diethylammonium DETC were purchased from Sigma-Aldrich, DTT (ultrapure) from USB Corporation, DLA from Santa Cruz Biotechnology.
Techniques: Binding Assay