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Thermo Fisher
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Millipore
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Thermo Fisher
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FUJIFILM
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Image Search Results

Journal: eLife
Article Title: Mutations in L-type amino acid transporter-2 support SLC7A8 as a novel gene involved in age-related hearing loss
doi: 10.7554/eLife.31511
Figure Lengend Snippet: Hearing phenotype of C57BL6/J-129Sv Slc7a8 knockout mice. ( A ) Representative image of western bloting of total membranes from kidney, brain and intestine of wild-type (+/+) and Slc7a8 knock out (-/-) mice in the absence (-) or presence (+) of 100 mM dithiothreitol reducing agent (DTT) of three independent biological samples for both sexes (male and female). Protein (50 μg) were loaded in 7% acrylamide SDS-PAGE gel. Molecular mass standard (KDa) are indicated. Red arrows point SLC7A8/CD98hc heterodimer band as well as the light subunit SLC7A8. Upper panel: Rabbit anti-SLC7A8. Bottom panel: Mouse anti-βactin. ( B ) Pre-Pulse Inhibition of the acoustic startle response (PPI). Mean and SEM are represented. Pulse: 120 dB single pulse. Pre-pulse inhibition test: six different pre-pulse intensities (70 to 90 dB) in pseudo random order with 15 s inter-trial intervals. Wild type (white circles, n = 19) and Slc7a8 −/− (blue circles, n = 15) from 4- to 7-month-old are represented. Significant differences were determined using paired Student’s t-test, ***p
Article Snippet: For specific SLC7A8 light subunit detection, samples were run in the presence of 100 mM of
Techniques: Knock-Out, Mouse Assay, Western Blot, SDS Page, Inhibition
![Involvement of the N-terminal cysteine residues of mouse TRPA1 in its activation by polysulfide. (A) The Na 2 S 3 (10 μM)-induced [Ca 2+ ] i increase was inhibited by dithiothreitol (DTT) (a) 2 min before and during 4 min application of Na 2 S 3 , (b) after 4 min in HEK 293 cells expressing mouse TRPA1 (mTRPA1-HEK). The upper panels show [Ca 2+ ] i responses to Na 2 S 3 without DTT, and the lower ones those in the presence of DTT. (B) Summarized effects of DTT. (a) Open and filled columns show the increases of [Ca 2+ ] i responses to Na 2 S 3 in the absence (Control) and presence of DTT, respectively. (b) Times required for half-decline of [Ca 2+ ] i responses to Na 2 S 3 (T 1/2 ) in the absence (Control) and presence of DTT. T 1/2 was calculated by subtracting the value of the time when the Na 2 S 3 -induced [Ca 2+ ] i increase was reduced by half from that when Na 2 S 3 -induced [Ca 2+ ] i increase peaked. Columns with vertical lines show mean ± SEM (a; n = 23–32, b; n = 55–63, from three different transfections). **P](https://storage.googleapis.com/bioz_article_images/PMC4428232/12990_2015_23_Fig7_HTML.jpg)
Journal: Molecular Pain
Article Title: Polysulfide evokes acute pain through the activation of nociceptive TRPA1 in mouse sensory neurons
doi: 10.1186/s12990-015-0023-4
Figure Lengend Snippet: Involvement of the N-terminal cysteine residues of mouse TRPA1 in its activation by polysulfide. (A) The Na 2 S 3 (10 μM)-induced [Ca 2+ ] i increase was inhibited by dithiothreitol (DTT) (a) 2 min before and during 4 min application of Na 2 S 3 , (b) after 4 min in HEK 293 cells expressing mouse TRPA1 (mTRPA1-HEK). The upper panels show [Ca 2+ ] i responses to Na 2 S 3 without DTT, and the lower ones those in the presence of DTT. (B) Summarized effects of DTT. (a) Open and filled columns show the increases of [Ca 2+ ] i responses to Na 2 S 3 in the absence (Control) and presence of DTT, respectively. (b) Times required for half-decline of [Ca 2+ ] i responses to Na 2 S 3 (T 1/2 ) in the absence (Control) and presence of DTT. T 1/2 was calculated by subtracting the value of the time when the Na 2 S 3 -induced [Ca 2+ ] i increase was reduced by half from that when Na 2 S 3 -induced [Ca 2+ ] i increase peaked. Columns with vertical lines show mean ± SEM (a; n = 23–32, b; n = 55–63, from three different transfections). **P
Article Snippet:
Techniques: Activation Assay, Expressing, Transfection