Journal: Molecular cancer research : MCR
Article Title: Increased expression of Beige/Brown adipose markers from host and breast cancer cells influence xenograft formation in mice
Figure Lengend Snippet: Presence of cells with brown adipocyte phenotype and characteristics in HMLE HRASV12 xenografts over time. A–B, Xenografts at various stages of growth were fixed with glutaraldehyde, post-fixed with osmium tetroxide and embedded in Technovit. Low (A) and high (B) magnification pictures of hematoxylin/eosin-stained sections are shown. Cells (2×10 6 ) from a transplantable HMLE HRASV12 xenograft were mixed with matrigel (1:1) and implanted at a subcutaneous site (posterior dorsolateral) in nude mice to develop xenografts, which were excised at various time points. C, Representative bright field images of xenografts at 1, 5, and 15 week. D, Western blot analysis of pooled lysates (n=3 mice/time point) from xenografts excised at various time points after growth subcutaneously in nude mice. A representative western blot analysis is shown (n=3). E, FACS analysis of PRDM16 (1- and 3 week xenograft), and UCP1, and UCP3 (1- and 5 week xenograft) labeled cells. Data shown is a representation of multiple experiments (n=3). F, Western blot analysis of key markers of angiogenesis (VEGF), proliferation (cyclin D1), and extracellular matrix proteins (ColA1, SMA and FN) in NT and T xenografts. G, Analysis of cellular bioenergetics of cells (4×10 4 ) obtained from 7-and 15-week xenografts (n=3,*p
Article Snippet: A, Sections of xenografts were analyzed for expression of UCP1 and extracellular matrix proteins (ColA1, Col 3, SMA and FN) by double immunofluorescence: B, Cells from dissociated xenograft (5 week) were analyzed for co-expression of UCP1 (Texas Red) and adiponectin (FITC) by double immunofluorescence and a representative picture is presented.
Techniques: Staining, Mouse Assay, Western Blot, FACS, Labeling