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  • 97
    New England Biolabs drai
    Wild-type EXO1 and its E109K variant complement the MMR defect of EXO1-depleted extracts of HEK293 cells. ( A ) Schematic representation of the T/G MMR substrate. The <t>SalI</t> restriction site contains a T/G mismatch, which renders the site refractory to cleavage. Repair of the T/G mismatch to C/G restores a bona fide SalI site. The SalI- and the three <t>DraI</t> restriction sites are indicated. The restriction patterns seen upon agarose gel electrophoresis before and after repair are shown on the right. The Nt.BstNBI nicking site is located 316 nucleotides 5′ from the mispaired T. ( B ) Mismatch repair assay using HEK293 siEXO1 extracts supplemented with recombinant EXO1, either wild-type, or the E109K or D173A variants. The reactions were stopped after 30 min and the recovered substrates were digested with SalI/DraI. In the absence of repair, the substrate gives rise to fragments of 2484, 694 and 19 bp, while the repaired substrate generates fragments of 1324, 1160, 694 and 19 bp. The figure shows a scan of a 1% agarose gel stained with GelRed. The image is representative of three independent experiments.
    Drai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 443 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher drai sites
    Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with <t>DraI</t> and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of <t>SMN,</t> lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control
    Drai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher restriction enzyme drai
    Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with <t>DraI</t> and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of <t>SMN,</t> lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control
    Restriction Enzyme Drai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher fastdigest drai
    Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with <t>DraI</t> and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of <t>SMN,</t> lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control
    Fastdigest Drai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    AbbVie drais
    Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with <t>DraI</t> and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of <t>SMN,</t> lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control
    Drais, supplied by AbbVie, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher drai
    Restriction fragment length polymorphism (RFLP) analysis of Pneumocystis samples from Zurich, Switzerland. A – D , The RFLP pattern following agarose gel electrophoresis for the 10 samples that could be amplified for analysis. Labels at the top represent the individual samples. A and C , Gels were run following digestion with <t>DraI.</t> B and D , Gels were run following digestion with <t>Hpy188I.</t> Samples 1–6 and 14 are from renal transplant patients, and samples 7, 10, and 12 are from control patients. The letter G denotes a representative sample from the outbreak in Munich, Germany; + is a positive control. With both enzymes, the RFLP patterns of the renal transplant patients are identical to each other and to the German sample, whereas the control patients showed patterns that were different from each other as well as from the transplant patients. E and F , Southern blots of the gels from panels A and B , confirming the results of the gel analysis. Molecular weight markers are indicated on the left.
    Drai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Promega drai
    Dendrograms of <t>DraI</t> and <t>AseI</t> PFGE patterns of single isolates from 38 patients, M. abscessus T ATCC 19977, M. chelonae T ATCC 35752, M. immunogenum T ATCC 700505, M. massiliense T CCUG 48898, M. bolletii T ). Dendrograms were prepared using the BioNumerics (version 5.1) program by the Dice unweighted-pair group method with arithmetic means, based on 2% optimization and position tolerance. Isolates with highly similar (up to three band differences) PFGE patterns are boxed. Boxes A, isolates showing high degrees of similarity to surgical epidemic isolates; boxes B, indistinguishable patterns for isolates from three patients from different cities in SP; boxes C, isolates highly similar to a postinjection isolate from PA. Numbers at the upper left are percent similarity.
    Drai, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Galderma drais
    Dendrograms of <t>DraI</t> and <t>AseI</t> PFGE patterns of single isolates from 38 patients, M. abscessus T ATCC 19977, M. chelonae T ATCC 35752, M. immunogenum T ATCC 700505, M. massiliense T CCUG 48898, M. bolletii T ). Dendrograms were prepared using the BioNumerics (version 5.1) program by the Dice unweighted-pair group method with arithmetic means, based on 2% optimization and position tolerance. Isolates with highly similar (up to three band differences) PFGE patterns are boxed. Boxes A, isolates showing high degrees of similarity to surgical epidemic isolates; boxes B, indistinguishable patterns for isolates from three patients from different cities in SP; boxes C, isolates highly similar to a postinjection isolate from PA. Numbers at the upper left are percent similarity.
    Drais, supplied by Galderma, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa drai genomewalker library
    Dendrograms of <t>DraI</t> and <t>AseI</t> PFGE patterns of single isolates from 38 patients, M. abscessus T ATCC 19977, M. chelonae T ATCC 35752, M. immunogenum T ATCC 700505, M. massiliense T CCUG 48898, M. bolletii T ). Dendrograms were prepared using the BioNumerics (version 5.1) program by the Dice unweighted-pair group method with arithmetic means, based on 2% optimization and position tolerance. Isolates with highly similar (up to three band differences) PFGE patterns are boxed. Boxes A, isolates showing high degrees of similarity to surgical epidemic isolates; boxes B, indistinguishable patterns for isolates from three patients from different cities in SP; boxes C, isolates highly similar to a postinjection isolate from PA. Numbers at the upper left are percent similarity.
    Drai Genomewalker Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Wild-type EXO1 and its E109K variant complement the MMR defect of EXO1-depleted extracts of HEK293 cells. ( A ) Schematic representation of the T/G MMR substrate. The SalI restriction site contains a T/G mismatch, which renders the site refractory to cleavage. Repair of the T/G mismatch to C/G restores a bona fide SalI site. The SalI- and the three DraI restriction sites are indicated. The restriction patterns seen upon agarose gel electrophoresis before and after repair are shown on the right. The Nt.BstNBI nicking site is located 316 nucleotides 5′ from the mispaired T. ( B ) Mismatch repair assay using HEK293 siEXO1 extracts supplemented with recombinant EXO1, either wild-type, or the E109K or D173A variants. The reactions were stopped after 30 min and the recovered substrates were digested with SalI/DraI. In the absence of repair, the substrate gives rise to fragments of 2484, 694 and 19 bp, while the repaired substrate generates fragments of 1324, 1160, 694 and 19 bp. The figure shows a scan of a 1% agarose gel stained with GelRed. The image is representative of three independent experiments.

    Journal: Nucleic Acids Research

    Article Title: Biochemical characterization of a cancer-associated E109K missense variant of human exonuclease 1

    doi: 10.1093/nar/gku419

    Figure Lengend Snippet: Wild-type EXO1 and its E109K variant complement the MMR defect of EXO1-depleted extracts of HEK293 cells. ( A ) Schematic representation of the T/G MMR substrate. The SalI restriction site contains a T/G mismatch, which renders the site refractory to cleavage. Repair of the T/G mismatch to C/G restores a bona fide SalI site. The SalI- and the three DraI restriction sites are indicated. The restriction patterns seen upon agarose gel electrophoresis before and after repair are shown on the right. The Nt.BstNBI nicking site is located 316 nucleotides 5′ from the mispaired T. ( B ) Mismatch repair assay using HEK293 siEXO1 extracts supplemented with recombinant EXO1, either wild-type, or the E109K or D173A variants. The reactions were stopped after 30 min and the recovered substrates were digested with SalI/DraI. In the absence of repair, the substrate gives rise to fragments of 2484, 694 and 19 bp, while the repaired substrate generates fragments of 1324, 1160, 694 and 19 bp. The figure shows a scan of a 1% agarose gel stained with GelRed. The image is representative of three independent experiments.

    Article Snippet: The reactions were terminated by a 30-min incubation with a stop solution (final concentrations: 0.5 mM EDTA, 1.5% SDS (sodium dodecyl sulfate), 2.5 mg/ml proteinase K), cleaned up on a MinElute column (Qiagen), and the recovered phagemid was subjected to restriction digest with 6 U SalI and 20 U DraI (NEB).

    Techniques: Variant Assay, Agarose Gel Electrophoresis, Recombinant, Staining

    Expression of the 46-bp insertion and detection of the two types of 16S rRNA by RT-PCR. The strains analyzed are indicated at the top. (A) Lanes +, use of consensual primer pair 27f and 590r; lanes ins, use of an insertion-specific pair of primers, ins1 and 590r. (B) Lanes +, RT-PCR products obtained with the primers 27f and 590r; lanes Dra I, RT-PCR products obtained in + lanes digested by Dra I. Lanes − (A and B), negative controls performed on the RNA of strain PR17/sat without reverse transcription and with the primers 27f and 590r (negative controls were also obtained for other strains and for the primers ins1 and 590r but are not shown); lane M, 100-bp DNA ladder (Promega) as a molecular weight marker.

    Journal: Journal of Bacteriology

    Article Title: Atypical 16S rRNA Gene Copies in Ochrobactrum intermedium Strains Reveal a Large Genomic Rearrangement by Recombination between rrn Copies

    doi: 10.1128/JB.185.9.2901-2909.2003

    Figure Lengend Snippet: Expression of the 46-bp insertion and detection of the two types of 16S rRNA by RT-PCR. The strains analyzed are indicated at the top. (A) Lanes +, use of consensual primer pair 27f and 590r; lanes ins, use of an insertion-specific pair of primers, ins1 and 590r. (B) Lanes +, RT-PCR products obtained with the primers 27f and 590r; lanes Dra I, RT-PCR products obtained in + lanes digested by Dra I. Lanes − (A and B), negative controls performed on the RNA of strain PR17/sat without reverse transcription and with the primers 27f and 590r (negative controls were also obtained for other strains and for the primers ins1 and 590r but are not shown); lane M, 100-bp DNA ladder (Promega) as a molecular weight marker.

    Article Snippet: Restriction analysis of PCR products was done with 10 U of Dra I (New England Biolabs, Hertfordshire, United Kingdom) by following the supplier's recommendations. ) and with sequences deposited in the Ribosomal Database Project, version 7.0, by using SIMILARITY RANK and SUGGEST TREE ( ).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Molecular Weight, Marker

    Plasmid analysis of donor strains, recipients, and transconjugants. All donor and recipient strains contained the plasmids pYE854/pYV and pBR327, respectively. (A) An 0.8% agarose gel showing DraI restriction patterns of the plasmids. Lane M,

    Journal: Journal of Bacteriology

    Article Title: Genetic and Functional Properties of the Self-Transmissible Yersinia enterocolitica Plasmid pYE854, Which Mobilizes the Virulence Plasmid pYV ▿ Plasmid pYE854, Which Mobilizes the Virulence Plasmid pYV ▿ †

    doi: 10.1128/JB.01467-07

    Figure Lengend Snippet: Plasmid analysis of donor strains, recipients, and transconjugants. All donor and recipient strains contained the plasmids pYE854/pYV and pBR327, respectively. (A) An 0.8% agarose gel showing DraI restriction patterns of the plasmids. Lane M,

    Article Snippet: The insertion sites of the resistance genes were determined by cloning DraI restriction fragments of the pYE854 mutants with the help of the vector pLitmus38 (Apr ; New England Biolabs).

    Techniques: Plasmid Preparation, Agarose Gel Electrophoresis

    Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with DraI and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of SMN, lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control

    Journal: Advanced Biomedical Research

    Article Title: Genotype-phenotype correlation of survival motor neuron and neuronal apoptosis inhibitory protein genes in spinal muscular atrophy patients from Iran

    doi: 10.4103/2277-9175.125872

    Figure Lengend Snippet: Deletion detection of survival motor neuron 1 (Exon 7, 8) performed by digestion of polymerase chain reaction product with DraI and DdeI, respectively. Digestion results were analyzed on 3% agarose gel. Lane 1 and 2: Different patients without deletion in exon 7 of survival motor neuron1 (SMN1), lane 3: Patient with deletion in exon 7, lane 4: Positive control and lane 5: Normal control, Lane 6: 100 bp marker; Lane 7: Patient without deletion in exon 8 of SMN, lane 8: Patient with deletion in exon 8, lane 9: Normal control, lane 10: Positive control

    Article Snippet: Amplification of SMN exons was performed using the following conditions: 94°C for 5 min followed by 95°C for 1 min, 55°C for 1 min and 72°C for 1 min for 35 cycles and final extension at 72°C for 7 min. Polymerase chain reaction (PCR) products of centromeric and telomeric exon 7 and 8 of SMN gene were distinguished by digestion with 1 U restriction enzymes DraI and DdeI (Fermentas, Germany), respectively.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Positive Control, Marker

    RFLP analysis of the vanRSYWHBX region digested with Dra I and Pag I ( Bsp HI). M, λ/ Bst EII DNA molecular weight standard (Kucharczyk TE, Warsaw, Poland).

    Journal: Journal of Clinical Microbiology

    Article Title: Outbreak of Vancomycin-Resistant Enterococcus faecium of the Phenotype VanB in a Hospital in Warsaw, Poland: Probable Transmission of the Resistance Determinants into an Endemic Vancomycin-Susceptible Strain

    doi: 10.1128/JCM.39.5.1781-1787.2001

    Figure Lengend Snippet: RFLP analysis of the vanRSYWHBX region digested with Dra I and Pag I ( Bsp HI). M, λ/ Bst EII DNA molecular weight standard (Kucharczyk TE, Warsaw, Poland).

    Article Snippet: DNA preparations were digested with Dra I and Pag I restrictases (MBI Fermentas), separated in 1% agarose gels (SeaKem; FMC Bioproducts), and blotted onto a Hybond-N+ membrane (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) for hybridization with the vanB gene cluster probe.

    Techniques: Molecular Weight

    REAP analysis (A) and hybridization of plasmid DNA digested with Dra I and Pag I ( Bsp HI) with the vanB gene cluster probe (B). M, λ/ Hin dIII DNA molecular weight standard (Kucharczyk TE, Warsaw, Poland). Arrows indicate DNA bands that differentiated the type I and type II polymorphs of the Tn 1547 -like element insertion locus.

    Journal: Journal of Clinical Microbiology

    Article Title: Outbreak of Vancomycin-Resistant Enterococcus faecium of the Phenotype VanB in a Hospital in Warsaw, Poland: Probable Transmission of the Resistance Determinants into an Endemic Vancomycin-Susceptible Strain

    doi: 10.1128/JCM.39.5.1781-1787.2001

    Figure Lengend Snippet: REAP analysis (A) and hybridization of plasmid DNA digested with Dra I and Pag I ( Bsp HI) with the vanB gene cluster probe (B). M, λ/ Hin dIII DNA molecular weight standard (Kucharczyk TE, Warsaw, Poland). Arrows indicate DNA bands that differentiated the type I and type II polymorphs of the Tn 1547 -like element insertion locus.

    Article Snippet: DNA preparations were digested with Dra I and Pag I restrictases (MBI Fermentas), separated in 1% agarose gels (SeaKem; FMC Bioproducts), and blotted onto a Hybond-N+ membrane (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) for hybridization with the vanB gene cluster probe.

    Techniques: Hybridization, Plasmid Preparation, Molecular Weight

    Restriction fragment length polymorphism (RFLP) analysis of Pneumocystis samples from Zurich, Switzerland. A – D , The RFLP pattern following agarose gel electrophoresis for the 10 samples that could be amplified for analysis. Labels at the top represent the individual samples. A and C , Gels were run following digestion with DraI. B and D , Gels were run following digestion with Hpy188I. Samples 1–6 and 14 are from renal transplant patients, and samples 7, 10, and 12 are from control patients. The letter G denotes a representative sample from the outbreak in Munich, Germany; + is a positive control. With both enzymes, the RFLP patterns of the renal transplant patients are identical to each other and to the German sample, whereas the control patients showed patterns that were different from each other as well as from the transplant patients. E and F , Southern blots of the gels from panels A and B , confirming the results of the gel analysis. Molecular weight markers are indicated on the left.

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Outbreaks of Pneumocystis Pneumonia in 2 Renal Transplant Centers Linked to a Single Strain of Pneumocystis: Implications for Transmission and Virulence

    doi: 10.1093/cid/cis217

    Figure Lengend Snippet: Restriction fragment length polymorphism (RFLP) analysis of Pneumocystis samples from Zurich, Switzerland. A – D , The RFLP pattern following agarose gel electrophoresis for the 10 samples that could be amplified for analysis. Labels at the top represent the individual samples. A and C , Gels were run following digestion with DraI. B and D , Gels were run following digestion with Hpy188I. Samples 1–6 and 14 are from renal transplant patients, and samples 7, 10, and 12 are from control patients. The letter G denotes a representative sample from the outbreak in Munich, Germany; + is a positive control. With both enzymes, the RFLP patterns of the renal transplant patients are identical to each other and to the German sample, whereas the control patients showed patterns that were different from each other as well as from the transplant patients. E and F , Southern blots of the gels from panels A and B , confirming the results of the gel analysis. Molecular weight markers are indicated on the left.

    Article Snippet: PCR products were purified using the QuickStep 2 PCR Purification Kit (Edge BioSystems, Gaithersburg, Maryland), digested with DraI and Hpy188I restriction enzymes for 6 hours at 37°C, and analyzed on a 1% or 2% tris-borate-ethylenediaminetetraacetic acid agarose gel following staining with SYBR green (Molecular Probes, Eugene, Oregon), as well as by Southern blotting.

    Techniques: Agarose Gel Electrophoresis, Amplification, Positive Control, Molecular Weight

    Restriction fragment length polymorphism (RFLP) analysis of Pneumocystis samples from Nagoya, Japan. The RFLP pattern following agarose gel electrophoresis ( A ) (following digestion with DraI on the left and Hpy188I on the right) and following Southern blotting ( B ), for the 4 samples that could be amplified for analysis. Labels at the top represent the individual samples. All 4 samples (J1, J2, J5, and J8) are from renal transplant patients. The letter G denotes a representative sample from the outbreak in Munich, Germany; the letter S denotes a representative sample from the outbreak in Zurich, Switzerland; + is a positive control. Samples J1, J2, and J5 showed a pattern identical to each other but different from the G and S samples, whereas sample J8 was different from all other samples. Molecular weight markers are indicated on the left.

    Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

    Article Title: Outbreaks of Pneumocystis Pneumonia in 2 Renal Transplant Centers Linked to a Single Strain of Pneumocystis: Implications for Transmission and Virulence

    doi: 10.1093/cid/cis217

    Figure Lengend Snippet: Restriction fragment length polymorphism (RFLP) analysis of Pneumocystis samples from Nagoya, Japan. The RFLP pattern following agarose gel electrophoresis ( A ) (following digestion with DraI on the left and Hpy188I on the right) and following Southern blotting ( B ), for the 4 samples that could be amplified for analysis. Labels at the top represent the individual samples. All 4 samples (J1, J2, J5, and J8) are from renal transplant patients. The letter G denotes a representative sample from the outbreak in Munich, Germany; the letter S denotes a representative sample from the outbreak in Zurich, Switzerland; + is a positive control. Samples J1, J2, and J5 showed a pattern identical to each other but different from the G and S samples, whereas sample J8 was different from all other samples. Molecular weight markers are indicated on the left.

    Article Snippet: PCR products were purified using the QuickStep 2 PCR Purification Kit (Edge BioSystems, Gaithersburg, Maryland), digested with DraI and Hpy188I restriction enzymes for 6 hours at 37°C, and analyzed on a 1% or 2% tris-borate-ethylenediaminetetraacetic acid agarose gel following staining with SYBR green (Molecular Probes, Eugene, Oregon), as well as by Southern blotting.

    Techniques: Agarose Gel Electrophoresis, Southern Blot, Amplification, Positive Control, Molecular Weight

    Dendrograms of DraI and AseI PFGE patterns of single isolates from 38 patients, M. abscessus T ATCC 19977, M. chelonae T ATCC 35752, M. immunogenum T ATCC 700505, M. massiliense T CCUG 48898, M. bolletii T ). Dendrograms were prepared using the BioNumerics (version 5.1) program by the Dice unweighted-pair group method with arithmetic means, based on 2% optimization and position tolerance. Isolates with highly similar (up to three band differences) PFGE patterns are boxed. Boxes A, isolates showing high degrees of similarity to surgical epidemic isolates; boxes B, indistinguishable patterns for isolates from three patients from different cities in SP; boxes C, isolates highly similar to a postinjection isolate from PA. Numbers at the upper left are percent similarity.

    Journal: Journal of Clinical Microbiology

    Article Title: Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates ▿

    doi: 10.1128/JCM.01665-10

    Figure Lengend Snippet: Dendrograms of DraI and AseI PFGE patterns of single isolates from 38 patients, M. abscessus T ATCC 19977, M. chelonae T ATCC 35752, M. immunogenum T ATCC 700505, M. massiliense T CCUG 48898, M. bolletii T ). Dendrograms were prepared using the BioNumerics (version 5.1) program by the Dice unweighted-pair group method with arithmetic means, based on 2% optimization and position tolerance. Isolates with highly similar (up to three band differences) PFGE patterns are boxed. Boxes A, isolates showing high degrees of similarity to surgical epidemic isolates; boxes B, indistinguishable patterns for isolates from three patients from different cities in SP; boxes C, isolates highly similar to a postinjection isolate from PA. Numbers at the upper left are percent similarity.

    Article Snippet: DNA was isolated from the plug molds and digested with 30 U DraI (Promega, Madison, WI) or AseI (Fermentas, Vilnius, Lithuania) at 37°C overnight.

    Techniques:

    Reproducibility of PFGE patterns among multiple isolates from seven patients. Between two and five isolates from each patient were analyzed by PFGE with DraI and AseI. The patterns obtained for isolates from the same patient were indistinguishable, except for the two polymorphisms indicated by arrows.

    Journal: Journal of Clinical Microbiology

    Article Title: Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates ▿

    doi: 10.1128/JCM.01665-10

    Figure Lengend Snippet: Reproducibility of PFGE patterns among multiple isolates from seven patients. Between two and five isolates from each patient were analyzed by PFGE with DraI and AseI. The patterns obtained for isolates from the same patient were indistinguishable, except for the two polymorphisms indicated by arrows.

    Article Snippet: DNA was isolated from the plug molds and digested with 30 U DraI (Promega, Madison, WI) or AseI (Fermentas, Vilnius, Lithuania) at 37°C overnight.

    Techniques: