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  • 94
    Olympus dp71 digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 94/100, based on 3526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Olympus bx51 microscopic dp71 digital camera system
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Bx51 Microscopic Dp71 Digital Camera System, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Olympus dp71 universal digital camera attachment
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 Universal Digital Camera Attachment, supplied by Olympus, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Olympus camedia dp71 digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Camedia Dp71 Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 88/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Olympus dp71 12 5 mpa digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 12 5 Mpa Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 85/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Olympus dp71 ccd digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 Ccd Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Olympus dp71 charge coupled device digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 Charge Coupled Device Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Olympus model dp71 digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Model Dp71 Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Olympus bx 51 microscope dp71 digital camera software
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Bx 51 Microscope Dp71 Digital Camera Software, supplied by Olympus, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Olympus dp71 12 5 megapixel digital microscope camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 12 5 Megapixel Digital Microscope Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Olympus dp71 12 5 mp digital camera
    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a <t>DP71</t> digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)
    Dp71 12 5 Mp Digital Camera, supplied by Olympus, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)

    Journal: Biotechnology for Biofuels

    Article Title: A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

    doi: 10.1186/s13068-017-0850-9

    Figure Lengend Snippet: Transient EYFP gene expression as directed by SCBV21 and its deletions in sugarcane. a Schematic map of full-length SCBV21 [1816 base pair (bp)] and its deletions. Nucleotide (nt) 1 is the first nt at the 5′ end of SCBV21 . Deletions are indicated by dotted lines and their nt position of each deletion is indicated above the dotted line . The region between SCBV21 and EYFP , marked with an asterisk (*) in deletion A, is derived from the multicloning site of pGEM T-Easy vector and is removed from all deletion fragments. In deletions C and D, the guanine base (G) at nt 1816 was deleted during cloning. Three important restriction enzyme sites, Xho I, Stu I, and Nco I used for deletions are marked in deletion A. The two potential promoter regions (PPR1 and PPR2) of SCBV21, which are 632 bp apart from each other, are shown with unfilled square boxes . The approximate position of primers used to generate deletions is indicated with filled arrowheads . b , c Monitoring of transient EYFP expression as directed by SCBV21 and its deletions in sugarcane young leaf segments. Representative images were collected with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus) (×15 magnification) for 48 h post-DNA bombardment ( scale bar 2.0 mm). EYFP expression levels were scored as high (+++), medium (++), low (+), and none (−), based on the EYFP focus count and EYFP expression level (mean gray value × pixels)

    Article Snippet: Evaluation of EYFP expression Images of different tissues expressing EYFP were collected at 48 h post-DNA bombardment using a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus).

    Techniques: Expressing, Derivative Assay, Plasmid Preparation, Clone Assay

    Quantitative assessment of transient EYFP gene expression as directed by SCBV21 , Ubi1 , Pr4 , CaMV 35S or enhanced CaMV 35S (2×35S) in sugarcane young leaf segments. Values of a EYFP focus count and b total EYFP expression level (10 4 ) (mean gray value × pixels) were collected from representative images monitored with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus) (×15 magnification) at 48 h post-DNA bombardment and calculated using ImageJ software as described in “ Methods ”. Values represent means with standard error from three independent experiments and nine replicates per experiment. Means with the same letter are not significantly different at p > 0.05

    Journal: Biotechnology for Biofuels

    Article Title: A novel Sugarcane bacilliform virus promoter confers gene expression preferentially in the vascular bundle and storage parenchyma of the sugarcane culm

    doi: 10.1186/s13068-017-0850-9

    Figure Lengend Snippet: Quantitative assessment of transient EYFP gene expression as directed by SCBV21 , Ubi1 , Pr4 , CaMV 35S or enhanced CaMV 35S (2×35S) in sugarcane young leaf segments. Values of a EYFP focus count and b total EYFP expression level (10 4 ) (mean gray value × pixels) were collected from representative images monitored with a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus) (×15 magnification) at 48 h post-DNA bombardment and calculated using ImageJ software as described in “ Methods ”. Values represent means with standard error from three independent experiments and nine replicates per experiment. Means with the same letter are not significantly different at p > 0.05

    Article Snippet: Evaluation of EYFP expression Images of different tissues expressing EYFP were collected at 48 h post-DNA bombardment using a stereomicroscope (Olympus SZX7, Olympus) fitted with YFPHQ filters (excitation of 490–500 nm and emission of 515–560 nm) and a DP71 digital camera (Olympus).

    Techniques: Expressing, Software

    Co-localization of ox-HDL and CD36 and effects of ox-HDL on CD36 expression in EPCs. EPCs were first incubated with either Dil-labeled or nonlabeled ox-HDL (both at the concentration of 50 μg/ml) for 8 h; then, CD36 was stained with AF488-linked antibody and DAPI. After being fixed with 4% formaldehyde, images of co-localization of ox-HDL and CD36 were captured by an Olympus inverted fluorescent microscope with a DP71 digital camera and then analyzed using Image J software (A) . Ox-HDL was shown to promote CD36 mRNA and protein expression in EPCs, which could be attenuated by shRNA-mediated knockdown. CD36 mRNA (B) and protein (C) expression of EPCs was investigated by real-time PCR and Western blot. Data are shown as means and standard errors, * p

    Journal: Antioxidants & Redox Signaling

    Article Title: Oxidized High-Density Lipoprotein Impairs Endothelial Progenitor Cells' Function by Activation of CD36-MAPK-TSP-1 Pathways

    doi: 10.1089/ars.2013.5743

    Figure Lengend Snippet: Co-localization of ox-HDL and CD36 and effects of ox-HDL on CD36 expression in EPCs. EPCs were first incubated with either Dil-labeled or nonlabeled ox-HDL (both at the concentration of 50 μg/ml) for 8 h; then, CD36 was stained with AF488-linked antibody and DAPI. After being fixed with 4% formaldehyde, images of co-localization of ox-HDL and CD36 were captured by an Olympus inverted fluorescent microscope with a DP71 digital camera and then analyzed using Image J software (A) . Ox-HDL was shown to promote CD36 mRNA and protein expression in EPCs, which could be attenuated by shRNA-mediated knockdown. CD36 mRNA (B) and protein (C) expression of EPCs was investigated by real-time PCR and Western blot. Data are shown as means and standard errors, * p

    Article Snippet: The images were recorded using a computer-assisted Olympus fluorescence microscope with a digital camera DP71 (Olympus IX51; Olympus, Inc.) and analyzed with ImageJ (NIH).

    Techniques: Expressing, Incubation, Labeling, Concentration Assay, Staining, Microscopy, Software, shRNA, Real-time Polymerase Chain Reaction, Western Blot