Journal: PLoS Genetics
Article Title: WDR23 regulates NRF2 independently of KEAP1
Figure Lengend Snippet: WDR23 overexpression enhances chemotherapy toxicity. ( A-C ) Cells overexpressing GFP:WDR23 isoform 1 (pink) or GFP:WDR23 isoform 2 (orange) are more sensitive to increasing concentrations of ( A ) etoposide (25uM n = 12 each, 50um n = 8 each, 75um n = 8 each), ( B ) doxorubicin (1uM n = 12 each, 2uM n = 8 each), or ( C ) cisplatin (50uM n = 12 each, 75uM n = 8 each, 100uM n = 8 each) as compared to cells expressing GFP alone (black). ( D,E ) The percentage of cells carrying DNA double strand breaks (DSB), as indicated by dual phospho-H2Ax and phospho-ATM staining, is increased when WDR23 isoform 1 or WDR23 isoform 2 is overexpressed (Control n = 3, Iso 1 n = 3, Iso 2 n = 3) and ( E ) enhances DSB incidence following etoposide treatment (Control n = 3, Iso 1 n = 3, Iso 2 n = 3). ( F ) Cellular apoptosis (Annexin V positive) is increased in cells overexpressing WDR23 isoform 1 or WDR23 isoform 2 as compared to control cells overexpressing GFP alone (Control n = 3, Iso 1 n = 3, Iso 2 n = 3). Data are mean ± s.e.m.; one-tailed t -test relative to control GFP overexpression for each treatment condition. * P
Article Snippet: Forty-eight hours post-transfection, cells were treated with the indicated chemical: Etoposide (Cayman), Doxorubicin (Cayman), or Cisplatin (Cayman).
Techniques: Over Expression, Expressing, Staining, One-tailed Test