Journal: BMC Musculoskeletal Disorders
Article Title: Genetic polymorphisms in bone morphogenetic protein receptor type IA gene predisposes individuals to ossification of the posterior longitudinal ligament of the cervical spine via the smad signaling pathway
Figure Lengend Snippet: The BMPR-IA gene coding sequence and pGEM-T/BMPR-IA (WT) vector digested with Hind III/Bam HI enzyme were analyzed by 4% agarose gel electrophoresis, respectively. a The annealed ds-oligos of BMPR-IA gene coding sequence was analyzed by 4% agarose gel electrophoresis. Each ds oligo annealing reactant showed a detectable molecular weight band around 1599 bp, as expected for the length of the designed ds-oligos of BMPR-IA gene. Lane M: Marker, Lane B: BMPR-IA gene.0020 b After the pGEM-T/BMPR-IA (WT) vector had been digested with Hind III/Bam HI enzyme, the fragments for BMPR-IA cDNA (1599 bp) and the pGEM-T vector (3000 bp) were observed in all the PCR products as analyzed by 4% agarose gel electrophoresis, respectively. The results showed that the ds-oligos of the BMPR-IA gene cDNA were ligated into the pGEM-T vector. Lane M: Marker, Lane 1: pGEM-T/BMPR-IA (WT) vector, Lane 2: The pGEM-T vector and BMPR-IA cDNA
Article Snippet: The ds-oligos were inserted into the pGEM-T vector (Invitrogen, Carlsbad, CA, USA) using T4 DNA ligase.
Techniques: IA, Sequencing, Plasmid Preparation, Agarose Gel Electrophoresis, Molecular Weight, Marker, Polymerase Chain Reaction