donkey anti-rat igg Search Results


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  • 94
    Jackson Immuno donkey antirat
    Donkey Antirat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 21 article reviews
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    91
    Thermo Fisher donkey anti rat igg
    A ternary protein complex comprised of Gimap5, HSC70, and Mcl-1 or Bcl-xL. (A) Cell lysates from parental 70Z/3 cells (parental) or 70Z/3 cells that stably express Flag-tagged Gimap5 (Gimap5nFL) were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to Western blot analysis with the indicated antibodies. (B) Cell lysates from parental or Gimap5nFL 70Z/3 cells were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to SDS-PAGE and Coomassie blue staining. Proteins specifically immunoprecipitated with anti-Flag in cell lysates derived from Gimap5nFL 70Z/3 cells, but not parental 70Z/3 cells, were subjected to an in-gel tryptic digestion. The peptides derived from the proteins were subjected to MALDI-TOF mass spectrometry analysis. (C) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti–Mcl-1 or anti–Bcl-xL or control <t>IgG.</t> The immune complex was subjected to Western blot analysis with the indicated antibodies. (D) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti-HSC70. The immune complex was subjected to Western blot analysis with the indicated antibodies. (E) The cytosol and mitochondria fractions were extracted from parental or Gimap5nFL 70Z/3 cells and subjected to Western blot analysis with the indicated antibodies. Tom20 was used as a marker of mitochondria fraction. (F) Gimap5nFL 70Z/3 cells were applied to glass slides and subjected to immunofluoresence analyses with anti-Flag (green) with a combination of anti–Mcl-1 or anti-HSC70 (red). Data shown are representative of three (A and E) or two (C, D, and F) independent experiments.
    Donkey Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    The Jackson Laboratory donkey anti rat igg
    Comparable expression of MHC class II messenger RNA and protein in Danon and wild-type B-LCL. (a) Total RNA was extracted from various wild-type or Danon B-LCL, complementary DNA was synthesized, and quantitative reverse transcription–polymerase chain reaction analysis performed using primers specific for HLA-DRα chain or for GAPDH as a control. Data are representative of the fold difference in messenger RNA expression observed in three independent experiments. (b) Wild-type Frev or LAMP-2-deficient DB.DR4 cells were incubated with L243 antibody to detect total surface HLA-DR and then stained with a fluorescein isothiocyanate-conjugated F(ab′) 2 fragment of goat anti-mouse <t>IgG</t> secondary antibody. The mean fluorescence intensity (MFI) as measured by flow cytometry indicates the level of surface HLA-DR, and data are the average MFI of three independent experiments. (c) Cell lysates were prepared from LAMP-2-deficient DB.DR4 or wild-type Frev B-LCL, the proteins resolved by gel electrophoresis under non-reducing conditions to preserve MHC class II dimers, and immunoblotted with an antibody to HLA-DRα chain. Data are representative of at least five independent experiments. The ratios of HLA-DRαβ dimers to GAPDH as a loading control were 1·5 and 1·3 for DB.DR4 and Frev, respectively. (d) Various wild-type or Danon B-LCL were first permeabilized and then incubated with L243 or W6/32 antibody to detect total intracellular HLA-DR or MHC class I molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total surface or intracellular MHC class I or class II molecules. Data are the average MFI of three independent experiments. (e) Wild-type Priess and Frev expressing endogenous HLA-DR4 and wild-type 7C3.DR4 or LAMP-2-deficient DB.DR4 transfectants were incubated with 3.5.9-13F10 antibody to detect surface HLA-DR4β chains and then stained with a <t>Cy2-conjugated</t> F(ab′) 2 fragment of donkey anti-rat IgG secondary antibody. The MFI as measured by flow cytometry indicates the level of surface HLA-DR4, and data are representative of more than three independent experiments. (f) Various wild-type or Danon B-LCL were first permeabilized and then incubated with MaP.DM1 (top panel) or a monoclonal antibody to HLA-DO (bottom panel) to detect total intracellular HLA-DM or HLA-DO molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total intracellular HLA-DM or HLA-DO molecules. Data for HLA-DM staining are the average MFI of three independent experiments while the data for HLA-DO staining are a representative experiment.
    Donkey Anti Rat Igg, supplied by The Jackson Laboratory, used in various techniques. Bioz Stars score: 91/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Jackson Immuno donkey anti rat igg cy3
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Cy3, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore cy3 donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Cy3 Donkey Anti Rat Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher donkey anti rat igg alexa488
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Alexa488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Jackson Immuno donkey anti rat igg dylight549
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Dylight549, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bio-Rad donkey anti rat antibody
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher alexa568 donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Alexa568 Donkey Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam donkey anti rat antibody
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher donkey anti rat igg af488
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Af488, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher dylight650 donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Dylight650 Donkey Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam donkey anti rat igg dylight 594
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Dylight 594, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Abcam donkey anti rat 650
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat 650, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jackson Immuno affinipure donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Affinipure Donkey Anti Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher donkey anti rat igg af594
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Af594, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Jackson Immuno donkey anti rat igg apc
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg Apc, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cedarlane cy3 donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Cy3 Donkey Anti Rat Igg, supplied by Cedarlane, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Abcam donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Donkey Anti Rat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher alexa 594 donkey anti rat igg
    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit <t>IgG-Alexa</t> 488 and donkey anti-rat <t>IgG-Cy3.</t> In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p
    Alexa 594 Donkey Anti Rat Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A ternary protein complex comprised of Gimap5, HSC70, and Mcl-1 or Bcl-xL. (A) Cell lysates from parental 70Z/3 cells (parental) or 70Z/3 cells that stably express Flag-tagged Gimap5 (Gimap5nFL) were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to Western blot analysis with the indicated antibodies. (B) Cell lysates from parental or Gimap5nFL 70Z/3 cells were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to SDS-PAGE and Coomassie blue staining. Proteins specifically immunoprecipitated with anti-Flag in cell lysates derived from Gimap5nFL 70Z/3 cells, but not parental 70Z/3 cells, were subjected to an in-gel tryptic digestion. The peptides derived from the proteins were subjected to MALDI-TOF mass spectrometry analysis. (C) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti–Mcl-1 or anti–Bcl-xL or control IgG. The immune complex was subjected to Western blot analysis with the indicated antibodies. (D) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti-HSC70. The immune complex was subjected to Western blot analysis with the indicated antibodies. (E) The cytosol and mitochondria fractions were extracted from parental or Gimap5nFL 70Z/3 cells and subjected to Western blot analysis with the indicated antibodies. Tom20 was used as a marker of mitochondria fraction. (F) Gimap5nFL 70Z/3 cells were applied to glass slides and subjected to immunofluoresence analyses with anti-Flag (green) with a combination of anti–Mcl-1 or anti-HSC70 (red). Data shown are representative of three (A and E) or two (C, D, and F) independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: Critical role for Gimap5 in the survival of mouse hematopoietic stem and progenitor cells

    doi: 10.1084/jem.20101192

    Figure Lengend Snippet: A ternary protein complex comprised of Gimap5, HSC70, and Mcl-1 or Bcl-xL. (A) Cell lysates from parental 70Z/3 cells (parental) or 70Z/3 cells that stably express Flag-tagged Gimap5 (Gimap5nFL) were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to Western blot analysis with the indicated antibodies. (B) Cell lysates from parental or Gimap5nFL 70Z/3 cells were incubated with Sepharose beads conjugated with anti-Flag. The immune complex was subjected to SDS-PAGE and Coomassie blue staining. Proteins specifically immunoprecipitated with anti-Flag in cell lysates derived from Gimap5nFL 70Z/3 cells, but not parental 70Z/3 cells, were subjected to an in-gel tryptic digestion. The peptides derived from the proteins were subjected to MALDI-TOF mass spectrometry analysis. (C) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti–Mcl-1 or anti–Bcl-xL or control IgG. The immune complex was subjected to Western blot analysis with the indicated antibodies. (D) Cell lysates from parental or Gimap5nFL 70Z/3 cells were immunoprecipitated with anti-HSC70. The immune complex was subjected to Western blot analysis with the indicated antibodies. (E) The cytosol and mitochondria fractions were extracted from parental or Gimap5nFL 70Z/3 cells and subjected to Western blot analysis with the indicated antibodies. Tom20 was used as a marker of mitochondria fraction. (F) Gimap5nFL 70Z/3 cells were applied to glass slides and subjected to immunofluoresence analyses with anti-Flag (green) with a combination of anti–Mcl-1 or anti-HSC70 (red). Data shown are representative of three (A and E) or two (C, D, and F) independent experiments.

    Article Snippet: Slides were washed three times in PBS and incubated for additional 1 h at room temperature with PE-conjugated goat anti–rabbit IgG (SouthernBiotech), APC-conjugated goat anti–mouse IgG1 (BD), or donkey anti–rat IgG (eBioscience), respectively.

    Techniques: Stable Transfection, Incubation, Western Blot, SDS Page, Staining, Immunoprecipitation, Derivative Assay, Mass Spectrometry, Marker

    Comparable expression of MHC class II messenger RNA and protein in Danon and wild-type B-LCL. (a) Total RNA was extracted from various wild-type or Danon B-LCL, complementary DNA was synthesized, and quantitative reverse transcription–polymerase chain reaction analysis performed using primers specific for HLA-DRα chain or for GAPDH as a control. Data are representative of the fold difference in messenger RNA expression observed in three independent experiments. (b) Wild-type Frev or LAMP-2-deficient DB.DR4 cells were incubated with L243 antibody to detect total surface HLA-DR and then stained with a fluorescein isothiocyanate-conjugated F(ab′) 2 fragment of goat anti-mouse IgG secondary antibody. The mean fluorescence intensity (MFI) as measured by flow cytometry indicates the level of surface HLA-DR, and data are the average MFI of three independent experiments. (c) Cell lysates were prepared from LAMP-2-deficient DB.DR4 or wild-type Frev B-LCL, the proteins resolved by gel electrophoresis under non-reducing conditions to preserve MHC class II dimers, and immunoblotted with an antibody to HLA-DRα chain. Data are representative of at least five independent experiments. The ratios of HLA-DRαβ dimers to GAPDH as a loading control were 1·5 and 1·3 for DB.DR4 and Frev, respectively. (d) Various wild-type or Danon B-LCL were first permeabilized and then incubated with L243 or W6/32 antibody to detect total intracellular HLA-DR or MHC class I molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total surface or intracellular MHC class I or class II molecules. Data are the average MFI of three independent experiments. (e) Wild-type Priess and Frev expressing endogenous HLA-DR4 and wild-type 7C3.DR4 or LAMP-2-deficient DB.DR4 transfectants were incubated with 3.5.9-13F10 antibody to detect surface HLA-DR4β chains and then stained with a Cy2-conjugated F(ab′) 2 fragment of donkey anti-rat IgG secondary antibody. The MFI as measured by flow cytometry indicates the level of surface HLA-DR4, and data are representative of more than three independent experiments. (f) Various wild-type or Danon B-LCL were first permeabilized and then incubated with MaP.DM1 (top panel) or a monoclonal antibody to HLA-DO (bottom panel) to detect total intracellular HLA-DM or HLA-DO molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total intracellular HLA-DM or HLA-DO molecules. Data for HLA-DM staining are the average MFI of three independent experiments while the data for HLA-DO staining are a representative experiment.

    Journal: Immunology

    Article Title: LAMP-2-deficient human B cells exhibit altered MHC class II presentation of exogenous antigens

    doi: 10.1111/j.1365-2567.2010.03309.x

    Figure Lengend Snippet: Comparable expression of MHC class II messenger RNA and protein in Danon and wild-type B-LCL. (a) Total RNA was extracted from various wild-type or Danon B-LCL, complementary DNA was synthesized, and quantitative reverse transcription–polymerase chain reaction analysis performed using primers specific for HLA-DRα chain or for GAPDH as a control. Data are representative of the fold difference in messenger RNA expression observed in three independent experiments. (b) Wild-type Frev or LAMP-2-deficient DB.DR4 cells were incubated with L243 antibody to detect total surface HLA-DR and then stained with a fluorescein isothiocyanate-conjugated F(ab′) 2 fragment of goat anti-mouse IgG secondary antibody. The mean fluorescence intensity (MFI) as measured by flow cytometry indicates the level of surface HLA-DR, and data are the average MFI of three independent experiments. (c) Cell lysates were prepared from LAMP-2-deficient DB.DR4 or wild-type Frev B-LCL, the proteins resolved by gel electrophoresis under non-reducing conditions to preserve MHC class II dimers, and immunoblotted with an antibody to HLA-DRα chain. Data are representative of at least five independent experiments. The ratios of HLA-DRαβ dimers to GAPDH as a loading control were 1·5 and 1·3 for DB.DR4 and Frev, respectively. (d) Various wild-type or Danon B-LCL were first permeabilized and then incubated with L243 or W6/32 antibody to detect total intracellular HLA-DR or MHC class I molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total surface or intracellular MHC class I or class II molecules. Data are the average MFI of three independent experiments. (e) Wild-type Priess and Frev expressing endogenous HLA-DR4 and wild-type 7C3.DR4 or LAMP-2-deficient DB.DR4 transfectants were incubated with 3.5.9-13F10 antibody to detect surface HLA-DR4β chains and then stained with a Cy2-conjugated F(ab′) 2 fragment of donkey anti-rat IgG secondary antibody. The MFI as measured by flow cytometry indicates the level of surface HLA-DR4, and data are representative of more than three independent experiments. (f) Various wild-type or Danon B-LCL were first permeabilized and then incubated with MaP.DM1 (top panel) or a monoclonal antibody to HLA-DO (bottom panel) to detect total intracellular HLA-DM or HLA-DO molecules, respectively. Cells were then stained with a phycoerythrin-conjugated F(ab′) 2 fragment of rabbit anti-mouse immunoglobulin secondary antibody. The MFI as measured by flow cytometry indicates the levels of total intracellular HLA-DM or HLA-DO molecules. Data for HLA-DM staining are the average MFI of three independent experiments while the data for HLA-DO staining are a representative experiment.

    Article Snippet: For flow cytometry, the FITC-conjugated F(ab′)2 fragment of goat anti-mouse IgG and the Cy2-conjugated F(ab′)2 fragment of donkey anti-rat IgG were purchased from Jackson Laboratories.

    Techniques: Expressing, Synthesized, Reverse Transcription Polymerase Chain Reaction, RNA Expression, Incubation, Staining, Fluorescence, Flow Cytometry, Cytometry, Nucleic Acid Electrophoresis

    Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit IgG-Alexa 488 and donkey anti-rat IgG-Cy3. In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p

    Journal: PLoS ONE

    Article Title: Hypoxia Inducible Factor-2? Regulates the Development of Retinal Astrocytic Network by Maintaining Adequate Supply of Astrocyte Progenitors

    doi: 10.1371/journal.pone.0084736

    Figure Lengend Snippet: Depletion of Pax2 + astrocyte progenitors in Hif-2α f/f /GFAP Cre mice. A to D. Anti-Pax2 IF staining of retinas at P0 (A and B) and P4 (C and D). The abundance of Pax2 + cells was dramatically reduced in Hif-2α f/f /GFAP Cre mice at both P0 and P4. Scale bars are 500 µm. E to J. Double IF staining of P0 retinas with rabbit anti-Pax2 and rat anti-GFAP, followed by goat anti-rabbit IgG-Alexa 488 and donkey anti-rat IgG-Cy3. In Hif-2α f/f retinas, Pax2 + (green) are abundantly present in the vicinity of strongly GFAP + (red) optic nerve head, but mature GFAP + astrocytes are virtually absent. In Hif-2α f/f /GFAP Cre mice, Pax2 + cells less abundantly present off the optic nerve, accompanied by many GFAP + cells. Scale bars in E to J are 200 µm. K. Percentage (%) of retinal areas occupied by Pax2 + cells. n = 5. * p

    Article Snippet: Following incubation with primary antibodies, retinas were washed, and incubated overnight with appropriate secondary antibodies including goat anti-rabbit IgG-Alexa fluor®-488 (1∶200, Life Technologies), donkey anti-rat IgG-Cy3 (2 µg/ml, Jackson ImmunoResearch, , West Grove, PA), and donkey anti-goat IgG-Alexa fluor®-488.

    Techniques: Mouse Assay, Staining