dntps New England Biolabs Search Results


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  • 99
    New England Biolabs phusion hf dna polymerase new england biolabs
    Phusion Hf Dna Polymerase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs q5 high fidelity dna polymerase new england biolabs
    Q5 High Fidelity Dna Polymerase New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs deoxynucleotides
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    93
    New England Biolabs dntp
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Dntp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 6798 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dntp 6 primers
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
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    New England Biolabs deoxynucleotide triphosphates dntps
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Deoxynucleotide Triphosphates Dntps, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dntp solution
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
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    New England Biolabs nucleotides dntps
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Nucleotides Dntps, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs mm each dntps
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Mm Each Dntps, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dntp mixture
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Dntp Mixture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bsrdi
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Bsrdi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs terminal dntp transferase
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Terminal Dntp Transferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dntp free second strand synthesis buffer
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Dntp Free Second Strand Synthesis Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dpni
    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England <t>Biolabs).</t> (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard <t>dNTPs</t> or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).
    Dpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs msp i
    Hypermethylation of the C9orf72 promoter. a Cerebellar DNA from control ( n = 8, left ) and repeat-expanded cases ( n = 8, right ) were mock digested (no enzyme) or digested with Msp I, <t>Hpa</t> II or MspJ I. DNA was subject to repeat primed PCR and representative
    Msp I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bsmi
    Hypermethylation of the C9orf72 promoter. a Cerebellar DNA from control ( n = 8, left ) and repeat-expanded cases ( n = 8, right ) were mock digested (no enzyme) or digested with Msp I, <t>Hpa</t> II or MspJ I. DNA was subject to repeat primed PCR and representative
    Bsmi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs endoiv
    Hypermethylation of the C9orf72 promoter. a Cerebellar DNA from control ( n = 8, left ) and repeat-expanded cases ( n = 8, right ) were mock digested (no enzyme) or digested with Msp I, <t>Hpa</t> II or MspJ I. DNA was subject to repeat primed PCR and representative
    Endoiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs alu i
    <t>Alu</t> I digestion of amplicons from SARS-HCoV, HCoV-229E and HCoV-OC43. Lanes 1, 3 and 5 (MW): molecular weight markers (Amplisize ladder 50–2000 bp, Bio-Rad Laboratories, Mississauga, Canada); Lane 2: SARS-HCoV (predicted fragments: 126 bp, 94 bp); Lane 4: HCoV-229E (predicted fragments: 101 bp, 86 bp, 33 bp); Lane 6: HCoV-OC43 (predicted fragment: 220 bp).
    Alu I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 823 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard dNTPs or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).

    Journal: bioRxiv

    Article Title: Chemical transcription roadblocking for nascent RNA display

    doi: 10.1101/2019.12.26.888743

    Figure Lengend Snippet: Additional DNA template quality analyses (A) Non-denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (B) Non-denaturing PAGE of DNA templates in which the initial PCR amplification was split to perform translesion synthesis with either standard dNTPs or a thermostable dNTP mixture in which dATP and dCTP were substituted with 2-amino-dATP and 5-propynyl-dCTP. The size marker is the Quick-Load 100 bp DNA Ladder (New England Biolabs). (C) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification that was performed after several freeze-thaw cycles that occurred over the course of data collection. The size marker is the Low Range ssRNA Ladder (New England Biolabs). (D) Denaturing PAGE quality analysis of DNA template with an internal desthiobiotin-TEG modification and a 5’ biotin-TEG modification. The size marker is the Low Range ssRNA Ladder (New England Biolabs).

    Article Snippet: Briefly, five 100 μl reactions containing 81.5 μl of water, 10 μl Thermo Pol Buffer (New England Biolabs, Ipswich, MA), 2 μl of 10 mM dNTPs (New England Biolabs), 2.5 μl of 10 μM oligonucleotide A (unmodified forward primer; ), 2.5 μl of 10 μM oligonucleotide C (unmodified reverse primer; ) or oligonucleotide F (5’ biotinylated reverse primer, ), 1 μl of Vent Exo- DNA polymerase (New England Biolabs), and 0.5 μl of 0.1 nM oligonucleotide G (template oligonucleotide, ) were amplified for 30 PCR cycles.

    Techniques: Polyacrylamide Gel Electrophoresis, Modification, Marker, Polymerase Chain Reaction, Amplification, Translesion Synthesis

    Different DNA polymerases can be used during the second nick translation. E. coli DNA polymerase I (New England Biolabs) can be used in place of Taq DNA polymerase during nick translation with dNTPs and biotin-dUTP. Sequencing peaks are wider with Pol I when nearly identical protocols are used (the only difference being Pol I incubates at 37 °C), however if this is undesirable a shorter incubation time will result in more narrow peaks. Use of Pol I could potentially lead to lower background noise since, unlike Taq , it has 3’→5’ exonuclease ‘proofreading’ activity that results in a lower observed base mis-incorporation rate.

    Journal: bioRxiv

    Article Title: NickSeq for genome-wide strand-specific identification of DNA single-strand break sites with single nucleotide resolution

    doi: 10.1101/867937

    Figure Lengend Snippet: Different DNA polymerases can be used during the second nick translation. E. coli DNA polymerase I (New England Biolabs) can be used in place of Taq DNA polymerase during nick translation with dNTPs and biotin-dUTP. Sequencing peaks are wider with Pol I when nearly identical protocols are used (the only difference being Pol I incubates at 37 °C), however if this is undesirable a shorter incubation time will result in more narrow peaks. Use of Pol I could potentially lead to lower background noise since, unlike Taq , it has 3’→5’ exonuclease ‘proofreading’ activity that results in a lower observed base mis-incorporation rate.

    Article Snippet: Next, nick translation was performed with regular dNTPs (New England Biolabs) and biotin-11-dUTP (Thermo Scientific).

    Techniques: Nick Translation, Sequencing, Incubation, Activity Assay

    NickSeq overview and workflow. a) Chemical structures of the two degenerate nucleotides dPTP and dKTP in both of their tautomeric forms enabling their respective activities as universal pyrimidine and purine. Black dotted lines represent hydrogen bonds between nucleotides. b) During nick translation with only the two degenerate nucleotides, P residues (gold) are inserted across from A (blue) and G (purple) residues while K residues (cyan) are inserted across from C (green) and T (red) residues. c) Sequencing DNA fragments without nicks or that had nick translation occur with only regular dNTPs will not show a mutational signal (top). Sequencing DNA fragments that underwent nick translation with dPTP and dKTP will show a mutational signal that extends a few bases 3’ of the nick’s original location (bottom). d) Workflow used to perform NickSeq. Nick translations are performed consecutively with dPTP plus dKTP and then with regular dNTPs plus biotinylated dUTP. Of note, nick translation will move a nick from its original location but will not actually repair the nick. DNA is fragmented and streptavidin-based purification enriches for fragments around the original (pre-nick translation) sites of nicks. After PCR and sequencing, sequence coverage and mutational information allow for sensitive single nucleotide-resolved and strand-specific identification of nick sites.

    Journal: bioRxiv

    Article Title: NickSeq for genome-wide strand-specific identification of DNA single-strand break sites with single nucleotide resolution

    doi: 10.1101/867937

    Figure Lengend Snippet: NickSeq overview and workflow. a) Chemical structures of the two degenerate nucleotides dPTP and dKTP in both of their tautomeric forms enabling their respective activities as universal pyrimidine and purine. Black dotted lines represent hydrogen bonds between nucleotides. b) During nick translation with only the two degenerate nucleotides, P residues (gold) are inserted across from A (blue) and G (purple) residues while K residues (cyan) are inserted across from C (green) and T (red) residues. c) Sequencing DNA fragments without nicks or that had nick translation occur with only regular dNTPs will not show a mutational signal (top). Sequencing DNA fragments that underwent nick translation with dPTP and dKTP will show a mutational signal that extends a few bases 3’ of the nick’s original location (bottom). d) Workflow used to perform NickSeq. Nick translations are performed consecutively with dPTP plus dKTP and then with regular dNTPs plus biotinylated dUTP. Of note, nick translation will move a nick from its original location but will not actually repair the nick. DNA is fragmented and streptavidin-based purification enriches for fragments around the original (pre-nick translation) sites of nicks. After PCR and sequencing, sequence coverage and mutational information allow for sensitive single nucleotide-resolved and strand-specific identification of nick sites.

    Article Snippet: Next, nick translation was performed with regular dNTPs (New England Biolabs) and biotin-11-dUTP (Thermo Scientific).

    Techniques: Nick Translation, Sequencing, Purification, Polymerase Chain Reaction

    Sequencing coverage peaks widen with increasing dNTP and biotinylated dUTP concentrations used during nick translation. The optimal concentration of dNTPs during the second nick translation was determined to be 40 nM with a 1:5 ratio of biotinylated dUTP to dTTP (defined as 1x). At higher concentrations, the peaks in sequencing coverage around the nicks become wider and there is additional background at locations far from nicks.

    Journal: bioRxiv

    Article Title: NickSeq for genome-wide strand-specific identification of DNA single-strand break sites with single nucleotide resolution

    doi: 10.1101/867937

    Figure Lengend Snippet: Sequencing coverage peaks widen with increasing dNTP and biotinylated dUTP concentrations used during nick translation. The optimal concentration of dNTPs during the second nick translation was determined to be 40 nM with a 1:5 ratio of biotinylated dUTP to dTTP (defined as 1x). At higher concentrations, the peaks in sequencing coverage around the nicks become wider and there is additional background at locations far from nicks.

    Article Snippet: Next, nick translation was performed with regular dNTPs (New England Biolabs) and biotin-11-dUTP (Thermo Scientific).

    Techniques: Sequencing, Nick Translation, Concentration Assay

    Hypermethylation of the C9orf72 promoter. a Cerebellar DNA from control ( n = 8, left ) and repeat-expanded cases ( n = 8, right ) were mock digested (no enzyme) or digested with Msp I, Hpa II or MspJ I. DNA was subject to repeat primed PCR and representative

    Journal: Acta neuropathologica

    Article Title: C9orf72 hypermethylation protects against repeat expansion-associated pathology in ALS/FTD

    doi: 10.1007/s00401-014-1286-y

    Figure Lengend Snippet: Hypermethylation of the C9orf72 promoter. a Cerebellar DNA from control ( n = 8, left ) and repeat-expanded cases ( n = 8, right ) were mock digested (no enzyme) or digested with Msp I, Hpa II or MspJ I. DNA was subject to repeat primed PCR and representative

    Article Snippet: DNA (1 μg) from post-mortem cerebellar tissue was restriction enzyme digested for 4 h with Hpa II (10 U), Msp I (10 U), or MspJ I (4 U) (New England Biolabs) at 37°C followed by phenol:chloroform:isoamyl alcohol extraction.

    Techniques: Polymerase Chain Reaction

    Alu I digestion of amplicons from SARS-HCoV, HCoV-229E and HCoV-OC43. Lanes 1, 3 and 5 (MW): molecular weight markers (Amplisize ladder 50–2000 bp, Bio-Rad Laboratories, Mississauga, Canada); Lane 2: SARS-HCoV (predicted fragments: 126 bp, 94 bp); Lane 4: HCoV-229E (predicted fragments: 101 bp, 86 bp, 33 bp); Lane 6: HCoV-OC43 (predicted fragment: 220 bp).

    Journal: Journal of Virological Methods

    Article Title: Comprehensive detection and identification of human coronaviruses, including the SARS-associated coronavirus, with a single RT-PCR assay

    doi: 10.1016/j.jviromet.2004.07.008

    Figure Lengend Snippet: Alu I digestion of amplicons from SARS-HCoV, HCoV-229E and HCoV-OC43. Lanes 1, 3 and 5 (MW): molecular weight markers (Amplisize ladder 50–2000 bp, Bio-Rad Laboratories, Mississauga, Canada); Lane 2: SARS-HCoV (predicted fragments: 126 bp, 94 bp); Lane 4: HCoV-229E (predicted fragments: 101 bp, 86 bp, 33 bp); Lane 6: HCoV-OC43 (predicted fragment: 220 bp).

    Article Snippet: The digestion reaction consisted of 10 μl of the RT-PCR mixture, 1.5 μl of the 10× enzyme buffer, 1 μl of Alu I (10 U/μl, New England Biolabs) and 2.5 μl of ddH2 O for a total of 15 μl.

    Techniques: Molecular Weight