dntp mix Thermo Fisher Search Results


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  • 90
    Thermo Fisher dntp mix
    Vpx+ virus, dN and <t>dNTP</t> treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or <t>dNTPs,</t> and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 15421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 15421 article reviews
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    85
    Thermo Fisher geneamp deoxynucleotide triphosphate mix
    Vpx+ virus, dN and <t>dNTP</t> treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or <t>dNTPs,</t> and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    Geneamp Deoxynucleotide Triphosphate Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche dntp mix
    Vpx+ virus, dN and <t>dNTP</t> treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or <t>dNTPs,</t> and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    Dntp Mix, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 1432 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher mmol dntp mix
    Vpx+ virus, dN and <t>dNTP</t> treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or <t>dNTPs,</t> and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.
    Mmol Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher dutp dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dutp Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher kk2501 dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Kk2501 Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    75
    Thermo Fisher pcr dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Pcr Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher deoxynucleoside triphosphate mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Deoxynucleoside Triphosphate Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1530 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1530 article reviews
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    86
    Thermo Fisher aminoallyl dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Aminoallyl Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 20 article reviews
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    78
    Thermo Fisher edta dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Edta Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher nuclease free deionized h2 o dntp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Nuclease Free Deionized H2 O Dntp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher deoxynucleoside triphosphate mix solution
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Deoxynucleoside Triphosphate Mix Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher deoxynucleotide 5 triphosphate mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Deoxynucleotide 5 Triphosphate Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dntp dutp mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dntp Dutp Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dntp mix superscript ii rnaseh reverse transcriptase
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dntp Mix Superscript Ii Rnaseh Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher deoxyribonucleoside triphosphates dntps mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Deoxyribonucleoside Triphosphates Dntps Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher aminoacid dntp mix aa dntp
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Aminoacid Dntp Mix Aa Dntp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher geneamp dntp blend mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Geneamp Dntp Blend Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher dntp purepeak dna polymerase mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dntp Purepeak Dna Polymerase Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher taqman dntp master mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Taqman Dntp Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 7 article reviews
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    80
    Thermo Fisher dntp purepeak dna polymerization mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dntp Purepeak Dna Polymerization Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher dntps purepeak polymerization mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dntps Purepeak Polymerization Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dynazyme ii dna polymerase deoxynucleoside triphosphate mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dynazyme Ii Dna Polymerase Deoxynucleoside Triphosphate Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher naoh
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Naoh, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 2111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher first strand cdna synthesis kit
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 14585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher sali restriction site sequence
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Sali Restriction Site Sequence, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher dtt
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Dtt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 23611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher taqman gene expression master mix
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Taqman Gene Expression Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 9425 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pcr amplification
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Pcr Amplification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52385 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher deoxynucleotide triphosphates mixture
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Deoxynucleotide Triphosphates Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher rt pcr reagents
    PfuX7 accepts <t>dUTP</t> in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard <t>dNTPs</t> or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).
    Rt Pcr Reagents, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vpx+ virus, dN and dNTP treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or dNTPs, and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.

    Journal: Stem cell research

    Article Title: Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs

    doi: 10.1016/j.scr.2015.06.012

    Figure Lengend Snippet: Vpx+ virus, dN and dNTP treatments result in minor increases in the dNTP pool of HSPCs. MDMs (A, C and E) and freshly isolated HSPCs (B, D and F) were pretreated for 2 h with Vpx+/− viruses, dNs or dNTPs, and cultured for 3 days before cell lysis. Quantification of dNTPs was done by a single nucleotide incorporation assay (see “Material and methods” section). Fold induction was calculated based on the absolute values of vero- vs mock-treated samples (C–F). White dots represent Vpx− virus-pretreated samples, black dots represent Vpx+ virus-pretreated samples (A–B). White bars represent dN-pretreated samples, black bars represent dNTP-pretreated samples (C–F). Data are from three donors and bars represent mean ± SEM. Statistical analysis was done using two-tailed paired t-test.

    Article Snippet: THP-1 cells transduced with lentiviral vectors encoding SAMHD1 shRNA were selected by culturing in a medium containing 0.8 μg/ml puromycin. dNs consisted of a mixture of dA (D8668), dC (D0776), dG (D0901) and dT (T1895; all from Sigma-Aldrich). dNTPs (R0192) were purchased from Fermentas (Glen Burnie, MD).

    Techniques: Isolation, Cell Culture, Lysis, Two Tailed Test

    PfuX7 accepts dUTP in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard dNTPs or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).

    Journal: BMC Biotechnology

    Article Title: A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

    doi: 10.1186/1472-6750-10-21

    Figure Lengend Snippet: PfuX7 accepts dUTP in place of dTTP in PCR . Agarose gel electrophoresis of PCRs performed with the Phusion (S7) or the PfuX7 (×7) DNA polymerases and either standard dNTPs or with a dUTP/dNTP mix where dUTP replaces dTTP. The molecular marker (M) is kb+ (Invitrogen).

    Article Snippet: PCR with dUTP replacing dTTP was performed using standard Phusion (Finnzymes) reaction conditions as recommended by the manufacturer, using the dUTP/dNTPs mix (Fermentas) instead of dNTPs.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Marker