Article Title: In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes
Figure Lengend Snippet: Schematic design of the methods adapted to extract genomic DNA from light or dark-adapted pin oak ( Q. palustris ) leaves or leaves of other species rich in polysaccharides and secondary metabolites: ( a ) M1, using CTAB (based on Qiagen DNeasy Plant DNA extraction kit) and ( b ) M2, using phenol (based on MoBio Power Plant DNA extraction kit) for contaminant removal. Red box framing indicates steps specifically modified within this work, differing from kit manufacturer recommendations.
Article Snippet: The homogenates were further processed using modified steps from the standard protocols developed for the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and MoBio Power Plant Kit (MoBio, Carlsbad, CA, USA), as specified: Method # 1 (M1)—modified method based on the Qiagen DNeasy Plant Mini Kit: following sample disruption using the Mini-Beadbeater-24, samples were incubated at 75 °C for 10 min, instead of 65 °C as recommended by the manufacturer, mixing the sample thoroughly every 2 min using a vortex.
Techniques: DNA Extraction, Modification