Journal: The American Journal of Tropical Medicine and Hygiene
Article Title: A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care
Figure Lengend Snippet: Sensitivity of recombinase polymerase amplification–lateral flow (RPA-LF) to detect Leishmania infantum compared with real-time polymerase chain reaction (PCR) used as gold standard. Tenfold serial dilutions of L. infantum promastigotes in dog blood were extracted using Qiagen ® DNeasy blood and tissue kit and detected by real-time quantitative PCR (SYBRgreen) or RPA-LF. Parasite dilutions: 1 = 10 5 , 2 = 10 4 , 3 = 10 3 , 4 = 10 2 , 5 = 10, 6 = 1, and 7 = 0.1 parasites and Bl = uninfected dog blood. The top band is the control band; the lower band is the test band. This is a representative figure of two similar assays.
Article Snippet: DNA was isolated from blood or tissue samples using the QIAGEN DNeasy blood and tissue extraction kit (Qiagen, Valencia, CA) following the instructions of the vendor.
Techniques: Recombinase Polymerase Amplification, Flow Cytometry, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction