dnase-treated total rna Search Results


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  • 99
    Qiagen rnasefree dnase set
    Rnasefree Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 1241 article reviews
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    99
    Thermo Fisher dnase treated total rna
    RT-PCR confirmation of selected rat hepatic gene expression in normal and tumor cells in response to 18 hr arginine deprivation . Semi-quantitative Reverse Transcription Polymerase Chain Reaction was used to assess relative expression of rat LDLr, farnesyl diphosphate synthase (FDPS), GADD45, Insig-1, and GAPDH for cells cultured with (+) or without (-) arginine, Adult liver served as a positive control. <t>DNase</t> treated total <t>RNA</t> was reverse-transcribed with and without (negative control) reverse transcriptase. A . Electrophoresis of PCR products was performed in 2% agarose, 1× TAE gel and visualized by ethidium bromide staining; 1 kb DNA marker was used to verify size of the PCR products. B . Densitometry of gel bands was assessed via LabWorks Software and values were ratioed to GAPDH to provide relative comparisons between Arginine + and Arginine - conditions.
    Dnase Treated Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa dnase treated total rna
    RT-PCR confirmation of selected rat hepatic gene expression in normal and tumor cells in response to 18 hr arginine deprivation . Semi-quantitative Reverse Transcription Polymerase Chain Reaction was used to assess relative expression of rat LDLr, farnesyl diphosphate synthase (FDPS), GADD45, Insig-1, and GAPDH for cells cultured with (+) or without (-) arginine, Adult liver served as a positive control. <t>DNase</t> treated total <t>RNA</t> was reverse-transcribed with and without (negative control) reverse transcriptase. A . Electrophoresis of PCR products was performed in 2% agarose, 1× TAE gel and visualized by ethidium bromide staining; 1 kb DNA marker was used to verify size of the PCR products. B . Densitometry of gel bands was assessed via LabWorks Software and values were ratioed to GAPDH to provide relative comparisons between Arginine + and Arginine - conditions.
    Dnase Treated Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dnase treated total rna - by Bioz Stars, 2020-04
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    99
    Bio-Rad dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dnase treated total rna - by Bioz Stars, 2020-04
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    99
    Millipore dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher dnase treated invitrogen total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Invitrogen Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Illumina Inc dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 97/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Toyobo dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Toyobo, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Roche dnaase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnaase Treated Total Rna, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Boehringer Mannheim dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rna, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Roche dnase treated total rnas
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rnas, supplied by Roche, used in various techniques. Bioz Stars score: 87/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Zyagen dnase treated total rnas
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Treated Total Rnas, supplied by Zyagen, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Illumina Inc total rna preparations dnase treated
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Total Rna Preparations Dnase Treated, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega dnase promega treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Dnase Promega Treated Total Rna, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Roche turbo dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Turbo Dnase Treated Total Rna, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rnase free dnase treated total rna
    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) <t>DNA</t> was extracted from infected cells that were treated with <t>DNase</t> or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.
    Rnase Free Dnase Treated Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Derepressed genes are transcribed and enriched for active chromatin marks in WT TSCs. a Inactive-X profiles of H3K27me3 in WT TSCs of derepressed and non-derepressed genes in Eed –/– TSCs and of X-inactivation escapees in WT TSCs. b Inactive-X profiles for <t>DNAseI</t> hypersensitivity (DNAseI HS), <t>RNA</t> PolII occupancy, H3K27ac, H3K4me2, and H3K36me3. c Correlation between percent paternal-X expression in WT and Eed –/– TSCs. d Paternal-X contribution for all expressed X-linked genes in WT TSCs. The x-axis indicates the rank order of each gene from least to most paternal-X expression in WT TSCs. Gray dots indicate non-derepressed genes and red dots mark genes that are derepressed in Eed –/– TSCs. The y-axis depicts percent paternal-X expression for each gene. The expressed paternal X-linked genes can be divided into five quintiles. The derepressed genes are over-represented in quintile 4. e Table of number of genes and their percent paternal-X expression within the quintiles described in ( d ). f Left , boxplots of absolute paternal-X expression (as RNA-seq reads per kilobase of gene per million reads [RPKM]) for non-derepressed and derepressed genes in quintile 4 (3–6% paternal-X:total X-chromosomal expression). Derepressed genes exhibit a significantly higher median expression level than non-derepressed genes ( p
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    Derepressed genes are transcribed and enriched for active chromatin marks in WT TSCs. a Inactive-X profiles of H3K27me3 in WT TSCs of derepressed and non-derepressed genes in Eed –/– TSCs and of X-inactivation escapees in WT TSCs. b Inactive-X profiles for <t>DNAseI</t> hypersensitivity (DNAseI HS), <t>RNA</t> PolII occupancy, H3K27ac, H3K4me2, and H3K36me3. c Correlation between percent paternal-X expression in WT and Eed –/– TSCs. d Paternal-X contribution for all expressed X-linked genes in WT TSCs. The x-axis indicates the rank order of each gene from least to most paternal-X expression in WT TSCs. Gray dots indicate non-derepressed genes and red dots mark genes that are derepressed in Eed –/– TSCs. The y-axis depicts percent paternal-X expression for each gene. The expressed paternal X-linked genes can be divided into five quintiles. The derepressed genes are over-represented in quintile 4. e Table of number of genes and their percent paternal-X expression within the quintiles described in ( d ). f Left , boxplots of absolute paternal-X expression (as RNA-seq reads per kilobase of gene per million reads [RPKM]) for non-derepressed and derepressed genes in quintile 4 (3–6% paternal-X:total X-chromosomal expression). Derepressed genes exhibit a significantly higher median expression level than non-derepressed genes ( p
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    Derepressed genes are transcribed and enriched for active chromatin marks in WT TSCs. a Inactive-X profiles of H3K27me3 in WT TSCs of derepressed and non-derepressed genes in Eed –/– TSCs and of X-inactivation escapees in WT TSCs. b Inactive-X profiles for <t>DNAseI</t> hypersensitivity (DNAseI HS), <t>RNA</t> PolII occupancy, H3K27ac, H3K4me2, and H3K36me3. c Correlation between percent paternal-X expression in WT and Eed –/– TSCs. d Paternal-X contribution for all expressed X-linked genes in WT TSCs. The x-axis indicates the rank order of each gene from least to most paternal-X expression in WT TSCs. Gray dots indicate non-derepressed genes and red dots mark genes that are derepressed in Eed –/– TSCs. The y-axis depicts percent paternal-X expression for each gene. The expressed paternal X-linked genes can be divided into five quintiles. The derepressed genes are over-represented in quintile 4. e Table of number of genes and their percent paternal-X expression within the quintiles described in ( d ). f Left , boxplots of absolute paternal-X expression (as RNA-seq reads per kilobase of gene per million reads [RPKM]) for non-derepressed and derepressed genes in quintile 4 (3–6% paternal-X:total X-chromosomal expression). Derepressed genes exhibit a significantly higher median expression level than non-derepressed genes ( p
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    Image Search Results


    RT-PCR confirmation of selected rat hepatic gene expression in normal and tumor cells in response to 18 hr arginine deprivation . Semi-quantitative Reverse Transcription Polymerase Chain Reaction was used to assess relative expression of rat LDLr, farnesyl diphosphate synthase (FDPS), GADD45, Insig-1, and GAPDH for cells cultured with (+) or without (-) arginine, Adult liver served as a positive control. DNase treated total RNA was reverse-transcribed with and without (negative control) reverse transcriptase. A . Electrophoresis of PCR products was performed in 2% agarose, 1× TAE gel and visualized by ethidium bromide staining; 1 kb DNA marker was used to verify size of the PCR products. B . Densitometry of gel bands was assessed via LabWorks Software and values were ratioed to GAPDH to provide relative comparisons between Arginine + and Arginine - conditions.

    Journal: Nutrition & Metabolism

    Article Title: Short-term arginine deprivation results in large-scale modulation of hepatic gene expression in both normal and tumor cells: microarray bioinformatic analysis

    doi: 10.1186/1743-7075-3-37

    Figure Lengend Snippet: RT-PCR confirmation of selected rat hepatic gene expression in normal and tumor cells in response to 18 hr arginine deprivation . Semi-quantitative Reverse Transcription Polymerase Chain Reaction was used to assess relative expression of rat LDLr, farnesyl diphosphate synthase (FDPS), GADD45, Insig-1, and GAPDH for cells cultured with (+) or without (-) arginine, Adult liver served as a positive control. DNase treated total RNA was reverse-transcribed with and without (negative control) reverse transcriptase. A . Electrophoresis of PCR products was performed in 2% agarose, 1× TAE gel and visualized by ethidium bromide staining; 1 kb DNA marker was used to verify size of the PCR products. B . Densitometry of gel bands was assessed via LabWorks Software and values were ratioed to GAPDH to provide relative comparisons between Arginine + and Arginine - conditions.

    Article Snippet: DNase treated total RNA was reverse-transcribed with and without reverse transcriptase for 60 minutes at 50 C using Superscript III Reverse Transcriptase (Invitrogen, Carlsbad, CA), Oligo (dT)12–18 (Invitrogen, Carlsbad, CA), RNase OUT ribonuclease inhibitor (Invitrogen, Carlsbad, CA, and 10 mM DNTP set (Invitrogen, Carlsbad, CA.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Positive Control, Negative Control, Electrophoresis, Polymerase Chain Reaction, Staining, Marker, Software

    THRAP3 and BCLAF1 double knockdown leads to impaired splicing and export of DDR transcripts. ( A and B ) Expression levels of (A) pre-spliced BRCA2 ( Exon 18–Intron 18 ), FANCD2 ( Exon 36–Intron 36 ), FANCL ( Exon6–Intron 6 ), RAD51 ( Exon 6–Intron 6 ), PALB2 ( Exon 9–Intron 9 ) and GAPDH (Exon 3–Intron 3) mRNAs and (B) post-spliced BRCA2 ( Exon 18-Exon 19 ), FANCD2 ( Exon 36-Exon 37), FANCL ( Exon 6-Exon 7 ), RAD51 ( Exon 6-Exon 7 ), PALB2 ( Exon 9-Exon 10 ) and GAPDH (Exon 3–Exon 4) mRNAs. Expression was assessed via qRT-PCR on cDNA generated from DNAse treated nuclear RNA extracts and normalized to ACTB levels in the same sample. Graphs represent the mean of three independent experiments ±SEM. Significance of changes was assessed using Student’s two-tailed t- test with significant changes indicated by ∗∗ P

    Journal: Nucleic Acids Research

    Article Title: The RNA processing factors THRAP3 and BCLAF1 promote the DNA damage response through selective mRNA splicing and nuclear export

    doi: 10.1093/nar/gkx1046

    Figure Lengend Snippet: THRAP3 and BCLAF1 double knockdown leads to impaired splicing and export of DDR transcripts. ( A and B ) Expression levels of (A) pre-spliced BRCA2 ( Exon 18–Intron 18 ), FANCD2 ( Exon 36–Intron 36 ), FANCL ( Exon6–Intron 6 ), RAD51 ( Exon 6–Intron 6 ), PALB2 ( Exon 9–Intron 9 ) and GAPDH (Exon 3–Intron 3) mRNAs and (B) post-spliced BRCA2 ( Exon 18-Exon 19 ), FANCD2 ( Exon 36-Exon 37), FANCL ( Exon 6-Exon 7 ), RAD51 ( Exon 6-Exon 7 ), PALB2 ( Exon 9-Exon 10 ) and GAPDH (Exon 3–Exon 4) mRNAs. Expression was assessed via qRT-PCR on cDNA generated from DNAse treated nuclear RNA extracts and normalized to ACTB levels in the same sample. Graphs represent the mean of three independent experiments ±SEM. Significance of changes was assessed using Student’s two-tailed t- test with significant changes indicated by ∗∗ P

    Article Snippet: qRT-PCR and splicing analysis A total of 1 μg of DNAse (Invitrogen) treated RNA was used for cDNA synthesis using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Applied Science) according to the manufacturer’s instructions. qRT-PCR was performed using primers specific to each transcript or to ACTB mRNA, on both RT positive and RT negative generated cDNA.

    Techniques: Expressing, Quantitative RT-PCR, Generated, Two Tailed Test

    Depletion of THRAP3 and BCLAF1 results in deficient processing of transcripts encoding the ATM kinase. ( A ) Expression level of total post-spliced ATM mRNA in control (siCtrl) and THRAP3 (siTHRAP3), BCLAF1 (siBCLAF1) and double depleted cells (siTHRAP3/siBCLAF1). Primers were designed within exons 20–21 spanning intron 20. mRNA expression was assessed via qRT-PCR on cDNA generated from DNAse treated RNA samples and normalized to ACTB mRNA levels in the same sample. Graphs represent the mean normalized expression from three independent experiments ±SEM. Significance of changes was assessed using Student’s two-tailed t- test with significant changes indicated by ∗∗ P

    Journal: Nucleic Acids Research

    Article Title: The RNA processing factors THRAP3 and BCLAF1 promote the DNA damage response through selective mRNA splicing and nuclear export

    doi: 10.1093/nar/gkx1046

    Figure Lengend Snippet: Depletion of THRAP3 and BCLAF1 results in deficient processing of transcripts encoding the ATM kinase. ( A ) Expression level of total post-spliced ATM mRNA in control (siCtrl) and THRAP3 (siTHRAP3), BCLAF1 (siBCLAF1) and double depleted cells (siTHRAP3/siBCLAF1). Primers were designed within exons 20–21 spanning intron 20. mRNA expression was assessed via qRT-PCR on cDNA generated from DNAse treated RNA samples and normalized to ACTB mRNA levels in the same sample. Graphs represent the mean normalized expression from three independent experiments ±SEM. Significance of changes was assessed using Student’s two-tailed t- test with significant changes indicated by ∗∗ P

    Article Snippet: qRT-PCR and splicing analysis A total of 1 μg of DNAse (Invitrogen) treated RNA was used for cDNA synthesis using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Applied Science) according to the manufacturer’s instructions. qRT-PCR was performed using primers specific to each transcript or to ACTB mRNA, on both RT positive and RT negative generated cDNA.

    Techniques: Expressing, Quantitative RT-PCR, Generated, Two Tailed Test

    Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) DNA was extracted from infected cells that were treated with DNase or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.

    Journal: Journal of Virology

    Article Title: Cellular Cholesterol Facilitates the Postentry Replication Cycle of Herpes Simplex Virus 1

    doi: 10.1128/JVI.00445-17

    Figure Lengend Snippet: Cholesterol depletion affects the synthesis of viral proteins and the yield and release of infectious HSV-1. (A and B) Vero cells were infected with HSV-1 KOS (MOI = 5) for 1 h at 37°C. The cultures were mock treated (−) or treated with 50 mM MβCD (+) for 45 min at room temperature and then rinsed and replenished with serum-free medium. At 19 h p.i, (A) or the indicated times (B), lysates were separated by SDS-PAGE. The Western blots were probed with anti-HSV polyclonal antibody (A) or monoclonal antibodies to tubulin, ICP4, ICP8, and VP16 (B). (C to E) Densitometry of the HSV-1 proteins in panel B presented as the ratio of viral protein to tubulin. (F) Supernatant fractions or complete whole-cell lysates were collected at 19 h p.i., and viral titers were determined. The data are means of triplicate determinations with standard deviations and are representative of the results of at least three independent experiments. Numerical percentages of extracellular infectious HSV-1 are indicated. (G) DNA was extracted from infected cells that were treated with DNase or left untreated. Cell-associated HSV-1 genome copies were quantitated by qPCR. The data presented are means of the results of three independent experiments. Numerical percentages of copy numbers are indicated to the right of the bars. Student's t test; DNase resistance in untreated versus MβCD-treated cells; P = 0.032.

    Article Snippet: Samples were treated with 2 μg/ml DNase (Bio-Rad), and viral genomic DNA was extracted using a QIAamp Blood DNA kit (Qiagen, Valencia, CA).

    Techniques: Infection, SDS Page, Western Blot, Real-time Polymerase Chain Reaction

    NuMA interacts with proteins and nucleic acid components of ribosomal biogenesis. S1 cells were cultured for 8 days with complete medium followed by 2 days without EGF to induce proliferation arrest; T4–2 cells were cultured for 6 days. ( A ) Immunoprecipitation (IP) of nuclear extracts from S1 and T4–2 cells with NuMA antibodies (NuMA) or with non-specific immunoglobulins (IgG), followed by western blot analysis of the input and immunoprecipitated samples using NuMA and NM1 antibodies. ( B ) Immunoprecipitation of nuclear extracts from S1 cells followed by western blot analysis for NuMA and RNA polymerase I (RNA pol I). ( C ) ChIP from T4–2 cells with NuMA antibody ( n = 4) or RNA Polymerase I antibody ( n = 2), followed by RT-qPCR for coding (pro-1, H4, H8, H13) and non-coding (H18) regions of rDNA. The drawing shows the organization of the rDNA gene (IGS = intergenic sequence). Data are normalized to those obtained with IgG control (see ‘Materials and Methods’ section). ( D ) RNA immunoprecipitation fromT4–2 cells with NuMA antibody or with non-specific IgG of nuclear extracts and soluble extracts (the latter were treated with 50 μg/ml cycloheximide [CH]). Both the DNAse treated non-reverse transcribed RNA (nonRT RNA) and the DNAse treated reverse-transcribed cDNA (cDNA) samples were subjected to PCR using primers specific for human 18S and 28S rRNAs ( n = 2). ( E and F ) Immunoprecipitation of nuclear extracts from S1 cells followed by western blot analysis for NuMA, RPL26 and RPL24.

    Journal: Nucleic Acids Research

    Article Title: The nuclear mitotic apparatus protein NuMA controls rDNA transcription and mediates the nucleolar stress response in a p53-independent manner

    doi: 10.1093/nar/gkx782

    Figure Lengend Snippet: NuMA interacts with proteins and nucleic acid components of ribosomal biogenesis. S1 cells were cultured for 8 days with complete medium followed by 2 days without EGF to induce proliferation arrest; T4–2 cells were cultured for 6 days. ( A ) Immunoprecipitation (IP) of nuclear extracts from S1 and T4–2 cells with NuMA antibodies (NuMA) or with non-specific immunoglobulins (IgG), followed by western blot analysis of the input and immunoprecipitated samples using NuMA and NM1 antibodies. ( B ) Immunoprecipitation of nuclear extracts from S1 cells followed by western blot analysis for NuMA and RNA polymerase I (RNA pol I). ( C ) ChIP from T4–2 cells with NuMA antibody ( n = 4) or RNA Polymerase I antibody ( n = 2), followed by RT-qPCR for coding (pro-1, H4, H8, H13) and non-coding (H18) regions of rDNA. The drawing shows the organization of the rDNA gene (IGS = intergenic sequence). Data are normalized to those obtained with IgG control (see ‘Materials and Methods’ section). ( D ) RNA immunoprecipitation fromT4–2 cells with NuMA antibody or with non-specific IgG of nuclear extracts and soluble extracts (the latter were treated with 50 μg/ml cycloheximide [CH]). Both the DNAse treated non-reverse transcribed RNA (nonRT RNA) and the DNAse treated reverse-transcribed cDNA (cDNA) samples were subjected to PCR using primers specific for human 18S and 28S rRNAs ( n = 2). ( E and F ) Immunoprecipitation of nuclear extracts from S1 cells followed by western blot analysis for NuMA, RPL26 and RPL24.

    Article Snippet: The DNAse-treated RNA sample was reverse-transcribed into cDNA using the Iscript™ DNA synthesis kit (Bio-Rad).

    Techniques: Cell Culture, Immunoprecipitation, Western Blot, Chromatin Immunoprecipitation, Quantitative RT-PCR, Sequencing, Polymerase Chain Reaction

    Derepressed genes are transcribed and enriched for active chromatin marks in WT TSCs. a Inactive-X profiles of H3K27me3 in WT TSCs of derepressed and non-derepressed genes in Eed –/– TSCs and of X-inactivation escapees in WT TSCs. b Inactive-X profiles for DNAseI hypersensitivity (DNAseI HS), RNA PolII occupancy, H3K27ac, H3K4me2, and H3K36me3. c Correlation between percent paternal-X expression in WT and Eed –/– TSCs. d Paternal-X contribution for all expressed X-linked genes in WT TSCs. The x-axis indicates the rank order of each gene from least to most paternal-X expression in WT TSCs. Gray dots indicate non-derepressed genes and red dots mark genes that are derepressed in Eed –/– TSCs. The y-axis depicts percent paternal-X expression for each gene. The expressed paternal X-linked genes can be divided into five quintiles. The derepressed genes are over-represented in quintile 4. e Table of number of genes and their percent paternal-X expression within the quintiles described in ( d ). f Left , boxplots of absolute paternal-X expression (as RNA-seq reads per kilobase of gene per million reads [RPKM]) for non-derepressed and derepressed genes in quintile 4 (3–6% paternal-X:total X-chromosomal expression). Derepressed genes exhibit a significantly higher median expression level than non-derepressed genes ( p

    Journal: Genome Biology

    Article Title: PRC2 represses transcribed genes on the imprinted inactive X chromosome in mice

    doi: 10.1186/s13059-017-1211-5

    Figure Lengend Snippet: Derepressed genes are transcribed and enriched for active chromatin marks in WT TSCs. a Inactive-X profiles of H3K27me3 in WT TSCs of derepressed and non-derepressed genes in Eed –/– TSCs and of X-inactivation escapees in WT TSCs. b Inactive-X profiles for DNAseI hypersensitivity (DNAseI HS), RNA PolII occupancy, H3K27ac, H3K4me2, and H3K36me3. c Correlation between percent paternal-X expression in WT and Eed –/– TSCs. d Paternal-X contribution for all expressed X-linked genes in WT TSCs. The x-axis indicates the rank order of each gene from least to most paternal-X expression in WT TSCs. Gray dots indicate non-derepressed genes and red dots mark genes that are derepressed in Eed –/– TSCs. The y-axis depicts percent paternal-X expression for each gene. The expressed paternal X-linked genes can be divided into five quintiles. The derepressed genes are over-represented in quintile 4. e Table of number of genes and their percent paternal-X expression within the quintiles described in ( d ). f Left , boxplots of absolute paternal-X expression (as RNA-seq reads per kilobase of gene per million reads [RPKM]) for non-derepressed and derepressed genes in quintile 4 (3–6% paternal-X:total X-chromosomal expression). Derepressed genes exhibit a significantly higher median expression level than non-derepressed genes ( p

    Article Snippet: A total of 0.5 μg of DNaseI-treated total RNA was used for cDNA synthesis using SuperScript III First-Strand synthesis system (Life Technologies, #18080) using a gene-specific reverse primer.

    Techniques: Expressing, RNA Sequencing Assay

    DNase I footprints of Eσ 70 in the presence or absence of CRP and CRP H159L on glnH p1 (non template strand). ( A ) Titration with increasing concentrations of CRP (lanes 2 and 7, 30 nM; lanes 3 and 8, 100 nM; lanes 4 and 9, 300 nM) were performed in the absence (2–4) or presence of 50 nM Eσ 70 (lanes 7–9). Lane 11 is A + G marker ladder. The protected regions were monitored by adding increasing concentrations of CRP in the presence or absence of Eσ 70 . ( B ) Titration with increasing concentrations of CRP (lane 3, 33 nM; lane 4, 100 nM; lane 5, 300 nM) and CRP H159L (lane 10, 33 nM; lane 11, 100 nM; lane 12, 300 nM) were performed in the presence of 25 nM Eσ 70 (lanes 3–5 and 10–12). Lane 7 is A + G marker ladder. The limits of protected regions are indicated. Note that wild-type CRP is recruited to a site centred at −61.5 by Eσ 70 -RNA polymerase, which suggests a higher affinity for DNA binding of Eσ 70 than that of CRP.

    Journal: Nucleic Acids Research

    Article Title: Interplay between CRP-cAMP and PII-Ntr systems forms novel regulatory network between carbon metabolism and nitrogen assimilation in Escherichia coli

    doi: 10.1093/nar/gkl1142

    Figure Lengend Snippet: DNase I footprints of Eσ 70 in the presence or absence of CRP and CRP H159L on glnH p1 (non template strand). ( A ) Titration with increasing concentrations of CRP (lanes 2 and 7, 30 nM; lanes 3 and 8, 100 nM; lanes 4 and 9, 300 nM) were performed in the absence (2–4) or presence of 50 nM Eσ 70 (lanes 7–9). Lane 11 is A + G marker ladder. The protected regions were monitored by adding increasing concentrations of CRP in the presence or absence of Eσ 70 . ( B ) Titration with increasing concentrations of CRP (lane 3, 33 nM; lane 4, 100 nM; lane 5, 300 nM) and CRP H159L (lane 10, 33 nM; lane 11, 100 nM; lane 12, 300 nM) were performed in the presence of 25 nM Eσ 70 (lanes 3–5 and 10–12). Lane 7 is A + G marker ladder. The limits of protected regions are indicated. Note that wild-type CRP is recruited to a site centred at −61.5 by Eσ 70 -RNA polymerase, which suggests a higher affinity for DNA binding of Eσ 70 than that of CRP.

    Article Snippet: About 1.5 μg of total DNase I-treated RNA were reverse-transcribed using SuperScriptII™ RT (Invitrogen) according to the manufacturer's instructions.

    Techniques: Titration, Marker, Binding Assay