Journal: PLoS Pathogens
Article Title: Targeting Neutrophils to Prevent Malaria-Associated Acute Lung Injury/Acute Respiratory Distress Syndrome in Mice
Figure Lengend Snippet: Recombinant human DNase 1 (Pulmozyme) and an elastase inhibitor (Sivelestat) improve outcomes of malaria-associated ALI/ARDS. (A) Lung sections of DBA/2 mice infected with 10 6 P . berghei ANKA-iRBCs on the day of death (9 th dpi) were stained for histone (red), myeloperoxidase (MPO) (green), and DNA (blue). Overlapping colors indicate cloud-like structures. White arrowheads show selected cells forming NETs (400x, scale bar 25 μm). (B) Survival, (C) parasitemia, (D) enhanced respiratory pause (Penh) and (E) respiratory frequency (RF) on the 7 th days post-infection (dpi) in P . berghei ANKA-infected mice treated with Pulmozyme (50 μg/mouse) or saline solution (control group) on the 3 rd and 6 th dpi. Data are representative of three independent experiments. (F) Survival, (G) parasitemia, (H) enhanced respiratory pause (Penh) and (I) respiratory frequency (RF) on the 7 th dpi in P . berghei ANKA-infected mice treated with elastase inhibitor (30 mg/kg) or saline solution (control group) on the 3 rd and 6 th dpi. Data are representative of one experiment. (J) Lung tissue on the day of death from representative control mice (died on the 10 th dpi with ALI/ARDS), Pulmozyme-treated mice (died on the 20 th dpi), and Sivelestat-treated mice (died on the 20 th dpi) stained with HE (400x, scale bar 25 μm). Data are expressed as the mean ± SEM (C, log-rank test and Wilcoxon-Gehan-Breslow, p
Article Snippet: Mice and anti-NET drug DNase Mice were anesthetized briefly with approximately 4% halothane and 96% oxygen and then administered 50 μg/mouse (5 mg/kg) of DNAse 1 (Pulmozyme, Roche, USA) or a saline solution via intranasal spray on the 3rd and 6th dpi as describe previously [ ].
Techniques: Recombinant, Mouse Assay, Infection, Staining