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  • 94
    Thermo Fisher dna thermal cycler
    Representative 2% agarose gels of <t>RAPD-PCR</t> patterns generated from 10 liver samples using three arbitrary primers: EZ: left, Chi 15 : middle and P 53 F: right . Lane M: <t>DNA</t> marker 1 kb Ladder, lane 1: control animal, lanes 2–5: NDEA-treated animals and lanes 6–10: NDEA+Q-treated animals.
    Dna Thermal Cycler, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    MJ Research dna thermal cycler
    <t>PCR-RFLP</t> analysis from the 439-bp segment of the HSP gene of the Streptomyces strain. Lane 1, 439-bp fragment amplified with TB11 and TB12 primers; lane 2, amplicon digested with MspI; lane 3, amplicon digested with HinfI; lane M, lambda <t>DNA</t> cleaved with
    Dna Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 92/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Bio-Rad dna thermal cycler
    <t>DNA</t> manipulation and <t>PCR</t> amplification.
    Dna Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad dna engine peltier thermal cycler
    <t>DNA</t> manipulation and <t>PCR</t> amplification.
    Dna Engine Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 772 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Eppendorf AG dna thermal cycler
    <t>PCR</t> products of isolates numbered 23 to 45 . Agarose gel electrophoresis of 933-bp PCR amplification products from chromosomal <t>DNA</t> from staphylococcal species using primers GF-1 and GR-2, M, +ve, -ve and black arrow are the same as in figure 1. Samples 23, 45: Staphylococcus hominis , 24-29: Staphylococcus epidermidis , 30, 31: Staphylococcus aureus , 32, 33: Staphylococcus lugdunensis , 34, 35: Staphylococcus warneri , 36, 37: Staphylococcus xlyosus and 38-42: Staphylococcus haemolyticus .
    Dna Thermal Cycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biometra dna thermal cycler
    <t>PCR</t> analysis showing amplified products ( a ) A1 – A3 represents the plants transformed by Agrobacterium tumefaciens and AH1 – AH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia annua ( b ) D1 – D3 represents the plants transformed by Agrobacterium tumefaciens and DH1 – DH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia dubia . Lane P represents the plasmid <t>DNA.</t> Lane C refers to the non transformed control plants. Lane M corresponds to 1 kbp Ladder (Fermentas)
    Dna Thermal Cycler, supplied by Biometra, used in various techniques. Bioz Stars score: 92/100, based on 314 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Rad dna engine dyad peltier thermal cycler
    The Hl gene encodes the tomato homolog of SRA1 . (A) Fine genetic mapping of Hl delimited the target gene to an interval between marker U601668 and T0675 on tomato chromosome 11. Numbers in parentheses indicate the number of recombination events identified between markers and the target gene. (B) Structure of SlSRA1 . Vertical black lines depict exons and horizontal lines indicate intervening introns or intergenic regions. The mutation identified in the hl mutant affects the last exon, denoted with an asterisk. The relative location and direction of transcription of annotated genes ( Solyc11g013290 and Solyc11g013280 ) is shown. (C) Agarose gel showing the results of <t>RT-PCR</t> amplification of SRA1 cDNAs using mRNA isolated from WT and hl leaves . Elongation factor 4A ( elF4A ) mRNA was used as a loading control. (D) Diagram depicting the nature of the deletion mutation identified in the hl mutant, as compared to the same genomic region of the WT. The hl mutation corresponds to a 3.2-kb deletion spanning the last exon of the gene. Arrows depict the directionality of short segments of <t>DNA</t> that are retained in the mutant. (E) Agarose gel showing the amplification products from 3ʹRACE of the SRA1 cDNA, using mRNA isolated from WT and hl leaves.
    Dna Engine Dyad Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Bio-Rad ptc 200 dna engine thermal cycler
    The Hl gene encodes the tomato homolog of SRA1 . (A) Fine genetic mapping of Hl delimited the target gene to an interval between marker U601668 and T0675 on tomato chromosome 11. Numbers in parentheses indicate the number of recombination events identified between markers and the target gene. (B) Structure of SlSRA1 . Vertical black lines depict exons and horizontal lines indicate intervening introns or intergenic regions. The mutation identified in the hl mutant affects the last exon, denoted with an asterisk. The relative location and direction of transcription of annotated genes ( Solyc11g013290 and Solyc11g013280 ) is shown. (C) Agarose gel showing the results of <t>RT-PCR</t> amplification of SRA1 cDNAs using mRNA isolated from WT and hl leaves . Elongation factor 4A ( elF4A ) mRNA was used as a loading control. (D) Diagram depicting the nature of the deletion mutation identified in the hl mutant, as compared to the same genomic region of the WT. The hl mutation corresponds to a 3.2-kb deletion spanning the last exon of the gene. Arrows depict the directionality of short segments of <t>DNA</t> that are retained in the mutant. (E) Agarose gel showing the amplification products from 3ʹRACE of the SRA1 cDNA, using mRNA isolated from WT and hl leaves.
    Ptc 200 Dna Engine Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 89/100, based on 252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MJ Research dna engine thermal cycler
    Comparison of PCR efficiency of Pfu and Pfu-S. λ <t>DNA</t> (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. M indicates molecular weight marker. PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 0.1% Triton-100, 2 mM MgCl 2 and 200 µM each dNTPs with 10 mM KCl for Pfu and 60 mM KCl for Pfu-S. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and <t>72°C</t> for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.
    Dna Engine Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 91/100, based on 209 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research dna engine dyad peltier thermal cycler
    Comparison of PCR efficiency of Pfu and Pfu-S. λ <t>DNA</t> (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. M indicates molecular weight marker. PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 0.1% Triton-100, 2 mM MgCl 2 and 200 µM each dNTPs with 10 mM KCl for Pfu and 60 mM KCl for Pfu-S. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and <t>72°C</t> for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.
    Dna Engine Dyad Peltier Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 91/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research dna engine opticon thermal cycler
    The role of LEDGF/p75 <t>DNA-binding</t> in stimulating HIV-1 integration in vitro . ( A ) Efficient use of pTZ18U/PL as an integration target. Reactions in lanes 2, 4–8, 10 and 12–16 contained 0.6 µM integrase, whereas reactions in lanes 3–8 and 11–16 contained 0.2 µM of the indicated protein. Reactions in lanes 9–16 additionally contained pTZ18U/PL. The migration positions of mini-HIV substrate DNA, supercoiled (s.c.) pTZ18U/PL and strand transfer reaction products are indicated. The migration pattern of the plasmid on its own is shown on the right; molecular mass standards are to the left. ( B ) Integrase (IN) alone, integration reaction conducted with integrase alone. Other reactions additionally contained 0.2 µM of the indicated protein. Relative levels of integration activity were quantified by real-time <t>PCR;</t> IN alone activity was arbitrarily set to 1.0. Error bars are variation obtained from duplicate real-time assays. Similar levels of wild-type and mutant LEDGF/p75 activities were observed following independent sets of integration reactions.
    Dna Engine Opticon Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 88/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad dna engine ptc 200 peltier thermal cycler
    The role of LEDGF/p75 <t>DNA-binding</t> in stimulating HIV-1 integration in vitro . ( A ) Efficient use of pTZ18U/PL as an integration target. Reactions in lanes 2, 4–8, 10 and 12–16 contained 0.6 µM integrase, whereas reactions in lanes 3–8 and 11–16 contained 0.2 µM of the indicated protein. Reactions in lanes 9–16 additionally contained pTZ18U/PL. The migration positions of mini-HIV substrate DNA, supercoiled (s.c.) pTZ18U/PL and strand transfer reaction products are indicated. The migration pattern of the plasmid on its own is shown on the right; molecular mass standards are to the left. ( B ) Integrase (IN) alone, integration reaction conducted with integrase alone. Other reactions additionally contained 0.2 µM of the indicated protein. Relative levels of integration activity were quantified by real-time <t>PCR;</t> IN alone activity was arbitrarily set to 1.0. Error bars are variation obtained from duplicate real-time assays. Similar levels of wild-type and mutant LEDGF/p75 activities were observed following independent sets of integration reactions.
    Dna Engine Ptc 200 Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MJ Research dna engine tetrad peltier thermal cycler
    The role of LEDGF/p75 <t>DNA-binding</t> in stimulating HIV-1 integration in vitro . ( A ) Efficient use of pTZ18U/PL as an integration target. Reactions in lanes 2, 4–8, 10 and 12–16 contained 0.6 µM integrase, whereas reactions in lanes 3–8 and 11–16 contained 0.2 µM of the indicated protein. Reactions in lanes 9–16 additionally contained pTZ18U/PL. The migration positions of mini-HIV substrate DNA, supercoiled (s.c.) pTZ18U/PL and strand transfer reaction products are indicated. The migration pattern of the plasmid on its own is shown on the right; molecular mass standards are to the left. ( B ) Integrase (IN) alone, integration reaction conducted with integrase alone. Other reactions additionally contained 0.2 µM of the indicated protein. Relative levels of integration activity were quantified by real-time <t>PCR;</t> IN alone activity was arbitrarily set to 1.0. Error bars are variation obtained from duplicate real-time assays. Similar levels of wild-type and mutant LEDGF/p75 activities were observed following independent sets of integration reactions.
    Dna Engine Tetrad Peltier Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 91/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    MJ Research programmable dna thermal cycler
    <t>PCR-based</t> electrophoretic outlines of individuals acquired from three mollusk species. <t>DNA</t> isolated from Notoacmea concinna (lane 1~7), Sulculus diversicolor supertexta (lane 8~14) and Haliotis discus hannai (lane 15~21) were amplified by decamer primers BION-55 (A), BION-50 (B), BION-75 (C), BION-35 (D), BION-61 (E), BION-69 (F) and BION-66 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. Each lane shows DNA samples extracted and purified from 21 individuals. 100 bp ladder was used as a DNA molecular size marker (M).
    Programmable Dna Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 88/100, based on 210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research ptc 200 dna engine thermal cycler
    <t>PCR-based</t> electrophoretic outlines of individuals acquired from three mollusk species. <t>DNA</t> isolated from Notoacmea concinna (lane 1~7), Sulculus diversicolor supertexta (lane 8~14) and Haliotis discus hannai (lane 15~21) were amplified by decamer primers BION-55 (A), BION-50 (B), BION-75 (C), BION-35 (D), BION-61 (E), BION-69 (F) and BION-66 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. Each lane shows DNA samples extracted and purified from 21 individuals. 100 bp ladder was used as a DNA molecular size marker (M).
    Ptc 200 Dna Engine Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 89/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research dna engine dyad thermal cycler
    <t>PCR-based</t> electrophoretic outlines of individuals acquired from three mollusk species. <t>DNA</t> isolated from Notoacmea concinna (lane 1~7), Sulculus diversicolor supertexta (lane 8~14) and Haliotis discus hannai (lane 15~21) were amplified by decamer primers BION-55 (A), BION-50 (B), BION-75 (C), BION-35 (D), BION-61 (E), BION-69 (F) and BION-66 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. Each lane shows DNA samples extracted and purified from 21 individuals. 100 bp ladder was used as a DNA molecular size marker (M).
    Dna Engine Dyad Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    MJ Research dna engine tetrad 2 thermal cycler
    Detection sensitivity of <t>multiplex-PCR-microarray</t> for EVs. The detection sensitivity of the assay was estimated for EVs (EV71) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant EV71 control; B. The microarray result after amplification and hybridization. M: 1 Kb <t>DNA</t> ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant EV71 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.
    Dna Engine Tetrad 2 Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 89/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad dna engine thermal cycler chromo4
    Detection sensitivity of <t>multiplex-PCR-microarray</t> for EVs. The detection sensitivity of the assay was estimated for EVs (EV71) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant EV71 control; B. The microarray result after amplification and hybridization. M: 1 Kb <t>DNA</t> ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant EV71 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.
    Dna Engine Thermal Cycler Chromo4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research dna engine ptc 200 peltier thermal cycler
    Detection sensitivity of <t>multiplex-PCR-microarray</t> for EVs. The detection sensitivity of the assay was estimated for EVs (EV71) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant EV71 control; B. The microarray result after amplification and hybridization. M: 1 Kb <t>DNA</t> ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant EV71 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.
    Dna Engine Ptc 200 Peltier Thermal Cycler, supplied by MJ Research, used in various techniques. Bioz Stars score: 88/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Representative 2% agarose gels of RAPD-PCR patterns generated from 10 liver samples using three arbitrary primers: EZ: left, Chi 15 : middle and P 53 F: right . Lane M: DNA marker 1 kb Ladder, lane 1: control animal, lanes 2–5: NDEA-treated animals and lanes 6–10: NDEA+Q-treated animals.

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Preventive effect of the flavonoid, quercetin, on hepatic cancer in rats via oxidant/antioxidant activity: molecular and histological evidences

    doi: 10.1186/1756-9966-28-80

    Figure Lengend Snippet: Representative 2% agarose gels of RAPD-PCR patterns generated from 10 liver samples using three arbitrary primers: EZ: left, Chi 15 : middle and P 53 F: right . Lane M: DNA marker 1 kb Ladder, lane 1: control animal, lanes 2–5: NDEA-treated animals and lanes 6–10: NDEA+Q-treated animals.

    Article Snippet: The amplification program used was 4 min at 94°C (hot start), 1 min at 94°C, 1 min at 30°C and 1 min at 72°C for 36 cycles followed by one cycle of 72°C for 10 min. PCR amplification was carried out in a DNA thermal cycler (Model 380 A, Applied Biosystems, CA, USA).

    Techniques: Polymerase Chain Reaction, Generated, Marker

    PCR amplification of p53 exon from liver tissues . Lane M: DNA marker, lane 1: control, lanes 2–4 NDEA-treated animals and lanes 8–9: NDEA+Q-treated animals.

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Preventive effect of the flavonoid, quercetin, on hepatic cancer in rats via oxidant/antioxidant activity: molecular and histological evidences

    doi: 10.1186/1756-9966-28-80

    Figure Lengend Snippet: PCR amplification of p53 exon from liver tissues . Lane M: DNA marker, lane 1: control, lanes 2–4 NDEA-treated animals and lanes 8–9: NDEA+Q-treated animals.

    Article Snippet: The amplification program used was 4 min at 94°C (hot start), 1 min at 94°C, 1 min at 30°C and 1 min at 72°C for 36 cycles followed by one cycle of 72°C for 10 min. PCR amplification was carried out in a DNA thermal cycler (Model 380 A, Applied Biosystems, CA, USA).

    Techniques: Polymerase Chain Reaction, Amplification, Marker

    PCR-RFLP analysis from the 439-bp segment of the HSP gene of the Streptomyces strain. Lane 1, 439-bp fragment amplified with TB11 and TB12 primers; lane 2, amplicon digested with MspI; lane 3, amplicon digested with HinfI; lane M, lambda DNA cleaved with

    Journal:

    Article Title: Streptomyces albus Isolated from a Human Actinomycetoma and Characterized by Molecular Techniques

    doi: 10.1128/JCM.42.12.5957-5960.2004

    Figure Lengend Snippet: PCR-RFLP analysis from the 439-bp segment of the HSP gene of the Streptomyces strain. Lane 1, 439-bp fragment amplified with TB11 and TB12 primers; lane 2, amplicon digested with MspI; lane 3, amplicon digested with HinfI; lane M, lambda DNA cleaved with

    Article Snippet: PCR amplification using a DNA thermal cycler (MJ Research, Inc.) included denaturation at 95°C for 5 min followed by 30 cycles at 95°C for 1 min, 60°C for 1 min, and 72°C for 1 min and a final incubation at 72°C for 10 min.

    Techniques: Polymerase Chain Reaction, Amplification, Lambda DNA Preparation

    DNA manipulation and PCR amplification.

    Journal: Applied and Environmental Microbiology

    Article Title: Sequencing and Transcriptional Analysis of the Biosynthesis Gene Cluster of Putrescine-Producing Lactococcus lactis ▿ ▿ †

    doi: 10.1128/AEM.05507-11

    Figure Lengend Snippet: DNA manipulation and PCR amplification.

    Article Snippet: PCR amplification was performed using a DNA thermal cycler (iCycler; Bio-Rad, Hercules, CA) for 35 cycles with the general program (94°C for 30 s, 25 s at the suitable annealing temperature of the primers used, and 72°C for 1 min per kb of DNA to be amplified).

    Techniques: Polymerase Chain Reaction, Amplification

    Reverse-transcription PCR and nested PCR for infectious bronchitis virus (IBV) detection and subtyping. Lanes M = 100-bp DNA ladder marker (Promega Corp., Madison, WI); lane 1 = negative control for IBV; lane 2 = XCE2+/XCE2−primers set for general detection of IBV (positive; band at 466 bp); lane 3 = Massachusetts type of IBV (positive; band at 295 bp); lane 4 = D274 type of IBV (positive; band at 217 bp); lane 5 = 4/91 type of IBV (positive; band at 154 bp).

    Journal: Poultry Science

    Article Title: Molecular Subtype of Infectious Bronchitis Virus in Broiler Flocks in Jordan

    doi: 10.3382/ps.2007-00509

    Figure Lengend Snippet: Reverse-transcription PCR and nested PCR for infectious bronchitis virus (IBV) detection and subtyping. Lanes M = 100-bp DNA ladder marker (Promega Corp., Madison, WI); lane 1 = negative control for IBV; lane 2 = XCE2+/XCE2−primers set for general detection of IBV (positive; band at 466 bp); lane 3 = Massachusetts type of IBV (positive; band at 295 bp); lane 4 = D274 type of IBV (positive; band at 217 bp); lane 5 = 4/91 type of IBV (positive; band at 154 bp).

    Article Snippet: Reverse transcription PCR was carried out in a DNA Engine thermal cycler (BioRad Laboratories Ltd., Mississauga, Ontario, Canada) for 1 reverse transcription cycle of 60 min at 45°C, followed by 94°C for 5 min, then 40 PCR cycles of 94°C for 45 s, 57°C for 45 s, and 72°C for 90 s, with a final extension cycle at 72°C for 5 min.

    Techniques: Polymerase Chain Reaction, Nested PCR, Marker, Negative Control

    PCR products of isolates numbered 23 to 45 . Agarose gel electrophoresis of 933-bp PCR amplification products from chromosomal DNA from staphylococcal species using primers GF-1 and GR-2, M, +ve, -ve and black arrow are the same as in figure 1. Samples 23, 45: Staphylococcus hominis , 24-29: Staphylococcus epidermidis , 30, 31: Staphylococcus aureus , 32, 33: Staphylococcus lugdunensis , 34, 35: Staphylococcus warneri , 36, 37: Staphylococcus xlyosus and 38-42: Staphylococcus haemolyticus .

    Journal: BMC Research Notes

    Article Title: High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program

    doi: 10.1186/1756-0500-3-278

    Figure Lengend Snippet: PCR products of isolates numbered 23 to 45 . Agarose gel electrophoresis of 933-bp PCR amplification products from chromosomal DNA from staphylococcal species using primers GF-1 and GR-2, M, +ve, -ve and black arrow are the same as in figure 1. Samples 23, 45: Staphylococcus hominis , 24-29: Staphylococcus epidermidis , 30, 31: Staphylococcus aureus , 32, 33: Staphylococcus lugdunensis , 34, 35: Staphylococcus warneri , 36, 37: Staphylococcus xlyosus and 38-42: Staphylococcus haemolyticus .

    Article Snippet: PCR amplification was carried out using DNA thermal cycler (Eppendorf, Gradient, Hamburg, Germany) by using PCR kit (PuReTaq Ready-To-Go™ PCR Beads from GE Healthcare, USA) following the manufacturer instructions.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification

    PCR products of isolates numbered 1 to 22 . Agarose gel electrophoresis of 933-bp PCR amplification products from chromosomal DNA from staphylococcal species using primers GF-1 and GR-2, M: Ladder marker, black arrow refers to the correct size product of the gap gene corresponding to 933 base pairs. +ve: Staphylococcus aureus ATCC 6538,-ve: Pseudomonas aeruginosa ATCC 9027. Samples 1-22 except 3 4: Staphylococcus hominis , 3: Staphylococcus aureus and 4: Staphylococcus sciuri .

    Journal: BMC Research Notes

    Article Title: High-throughput molecular identification of Staphylococcus spp. isolated from a clean room facility in an environmental monitoring program

    doi: 10.1186/1756-0500-3-278

    Figure Lengend Snippet: PCR products of isolates numbered 1 to 22 . Agarose gel electrophoresis of 933-bp PCR amplification products from chromosomal DNA from staphylococcal species using primers GF-1 and GR-2, M: Ladder marker, black arrow refers to the correct size product of the gap gene corresponding to 933 base pairs. +ve: Staphylococcus aureus ATCC 6538,-ve: Pseudomonas aeruginosa ATCC 9027. Samples 1-22 except 3 4: Staphylococcus hominis , 3: Staphylococcus aureus and 4: Staphylococcus sciuri .

    Article Snippet: PCR amplification was carried out using DNA thermal cycler (Eppendorf, Gradient, Hamburg, Germany) by using PCR kit (PuReTaq Ready-To-Go™ PCR Beads from GE Healthcare, USA) following the manufacturer instructions.

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Amplification, Marker

    PCR analysis showing amplified products ( a ) A1 – A3 represents the plants transformed by Agrobacterium tumefaciens and AH1 – AH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia annua ( b ) D1 – D3 represents the plants transformed by Agrobacterium tumefaciens and DH1 – DH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia dubia . Lane P represents the plasmid DNA. Lane C refers to the non transformed control plants. Lane M corresponds to 1 kbp Ladder (Fermentas)

    Journal: Malaria Journal

    Article Title: Cellular engineering of Artemisia annua and Artemisia dubia with the rol ABC genes for enhanced production of potent anti-malarial drug artemisinin

    doi: 10.1186/s12936-016-1312-8

    Figure Lengend Snippet: PCR analysis showing amplified products ( a ) A1 – A3 represents the plants transformed by Agrobacterium tumefaciens and AH1 – AH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia annua ( b ) D1 – D3 represents the plants transformed by Agrobacterium tumefaciens and DH1 – DH2 represents the plants transformed by Agrobacterium rhizogenes in Artemisia dubia . Lane P represents the plasmid DNA. Lane C refers to the non transformed control plants. Lane M corresponds to 1 kbp Ladder (Fermentas)

    Article Snippet: PCR analysis was performed using a programmed DNA thermal cycler (Biometra, USA).

    Techniques: Polymerase Chain Reaction, Amplification, Transformation Assay, Plasmid Preparation

    The Hl gene encodes the tomato homolog of SRA1 . (A) Fine genetic mapping of Hl delimited the target gene to an interval between marker U601668 and T0675 on tomato chromosome 11. Numbers in parentheses indicate the number of recombination events identified between markers and the target gene. (B) Structure of SlSRA1 . Vertical black lines depict exons and horizontal lines indicate intervening introns or intergenic regions. The mutation identified in the hl mutant affects the last exon, denoted with an asterisk. The relative location and direction of transcription of annotated genes ( Solyc11g013290 and Solyc11g013280 ) is shown. (C) Agarose gel showing the results of RT-PCR amplification of SRA1 cDNAs using mRNA isolated from WT and hl leaves . Elongation factor 4A ( elF4A ) mRNA was used as a loading control. (D) Diagram depicting the nature of the deletion mutation identified in the hl mutant, as compared to the same genomic region of the WT. The hl mutation corresponds to a 3.2-kb deletion spanning the last exon of the gene. Arrows depict the directionality of short segments of DNA that are retained in the mutant. (E) Agarose gel showing the amplification products from 3ʹRACE of the SRA1 cDNA, using mRNA isolated from WT and hl leaves.

    Journal: Journal of Experimental Botany

    Article Title: Molecular cloning of the tomato Hairless gene implicates actin dynamics in trichome-mediated defense and mechanical properties of stem tissue

    doi: 10.1093/jxb/erw292

    Figure Lengend Snippet: The Hl gene encodes the tomato homolog of SRA1 . (A) Fine genetic mapping of Hl delimited the target gene to an interval between marker U601668 and T0675 on tomato chromosome 11. Numbers in parentheses indicate the number of recombination events identified between markers and the target gene. (B) Structure of SlSRA1 . Vertical black lines depict exons and horizontal lines indicate intervening introns or intergenic regions. The mutation identified in the hl mutant affects the last exon, denoted with an asterisk. The relative location and direction of transcription of annotated genes ( Solyc11g013290 and Solyc11g013280 ) is shown. (C) Agarose gel showing the results of RT-PCR amplification of SRA1 cDNAs using mRNA isolated from WT and hl leaves . Elongation factor 4A ( elF4A ) mRNA was used as a loading control. (D) Diagram depicting the nature of the deletion mutation identified in the hl mutant, as compared to the same genomic region of the WT. The hl mutation corresponds to a 3.2-kb deletion spanning the last exon of the gene. Arrows depict the directionality of short segments of DNA that are retained in the mutant. (E) Agarose gel showing the amplification products from 3ʹRACE of the SRA1 cDNA, using mRNA isolated from WT and hl leaves.

    Article Snippet: PCR was performed with a DNA Engine Dyad Thermal Cycler (Bio-Rad).

    Techniques: Marker, Mutagenesis, Agarose Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Amplification, Isolation

    Comparison of PCR efficiency of Pfu and Pfu-S. λ DNA (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. M indicates molecular weight marker. PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 0.1% Triton-100, 2 mM MgCl 2 and 200 µM each dNTPs with 10 mM KCl for Pfu and 60 mM KCl for Pfu-S. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.

    Journal: Nucleic Acids Research

    Article Title: A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

    doi: 10.1093/nar/gkh271

    Figure Lengend Snippet: Comparison of PCR efficiency of Pfu and Pfu-S. λ DNA (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. M indicates molecular weight marker. PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 0.1% Triton-100, 2 mM MgCl 2 and 200 µM each dNTPs with 10 mM KCl for Pfu and 60 mM KCl for Pfu-S. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.

    Article Snippet: Primer annealing to the DNA template was achieved by heating at 90°C for 5 min, cooling to 72°C at 0.1°C/s, incubating at 72°C for 10 min, cooling again to 4°C at 0.1°C/s, using a DNA Engine thermal cycler (MJ Research).

    Techniques: Polymerase Chain Reaction, Molecular Weight, Marker

    Comparison of the salt tolerance of Sso7d fusions and the unmodified enzymes in PCR. λ DNA (130 pg/µl) was used as the template and a 0.9 kb amplicon was amplified in PCR buffer with increasing KCl concentrations. Lanes 1–12: 10, 20, 30, 40, 50, 60, 80, 100, 120, 140, 160 and 180 mM KCl. For Pfu and Pfu-S the PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 2 mM MgCl 2 , 0.1% Triton-100, 100 µg/ml BSA and 200 µM each dNTPs. For the other enzymes the PCR buffer contained 10 mM Tris–HCl pH 8.8, 2 mM MgCl 2 , 0.1% Triton-100 and 200 µM each dNTPs. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 60 s; 72°C for 10 min. M indicates molecular weight marker.

    Journal: Nucleic Acids Research

    Article Title: A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

    doi: 10.1093/nar/gkh271

    Figure Lengend Snippet: Comparison of the salt tolerance of Sso7d fusions and the unmodified enzymes in PCR. λ DNA (130 pg/µl) was used as the template and a 0.9 kb amplicon was amplified in PCR buffer with increasing KCl concentrations. Lanes 1–12: 10, 20, 30, 40, 50, 60, 80, 100, 120, 140, 160 and 180 mM KCl. For Pfu and Pfu-S the PCR buffer contained 20 mM Tris–HCl pH 8.8, 10 mM (NH 4 ) 2 SO 4 , 2 mM MgCl 2 , 0.1% Triton-100, 100 µg/ml BSA and 200 µM each dNTPs. For the other enzymes the PCR buffer contained 10 mM Tris–HCl pH 8.8, 2 mM MgCl 2 , 0.1% Triton-100 and 200 µM each dNTPs. The cycling protocol was 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 60 s; 72°C for 10 min. M indicates molecular weight marker.

    Article Snippet: Primer annealing to the DNA template was achieved by heating at 90°C for 5 min, cooling to 72°C at 0.1°C/s, incubating at 72°C for 10 min, cooling again to 4°C at 0.1°C/s, using a DNA Engine thermal cycler (MJ Research).

    Techniques: Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    Comparison of PCR efficiency of Taq, Taq(Δ289) and S-Taq(Δ289). λ DNA (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. The PCR buffer contained 10 mM Tris–HCl pH 8.8, 2 mM MgCl 2 , 200 µM each dNTPs and 0.1% Triton-100 with 10 mM KCl for Taq(Δ289) and 50 mM KCl for S-Taq(Δ289) and Taq. The cycling protocol was: 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.

    Journal: Nucleic Acids Research

    Article Title: A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro

    doi: 10.1093/nar/gkh271

    Figure Lengend Snippet: Comparison of PCR efficiency of Taq, Taq(Δ289) and S-Taq(Δ289). λ DNA (130 pg/µl) was used as the template and the sizes of the amplicons are indicated at the bottom. The PCR buffer contained 10 mM Tris–HCl pH 8.8, 2 mM MgCl 2 , 200 µM each dNTPs and 0.1% Triton-100 with 10 mM KCl for Taq(Δ289) and 50 mM KCl for S-Taq(Δ289) and Taq. The cycling protocol was: 95°C for 20 s; 20 cycles of 94°C for 5 s and 72°C for 30 s ( A ) or for 60 s ( B ) or for 2 min ( C ); 72°C for 7 min.

    Article Snippet: Primer annealing to the DNA template was achieved by heating at 90°C for 5 min, cooling to 72°C at 0.1°C/s, incubating at 72°C for 10 min, cooling again to 4°C at 0.1°C/s, using a DNA Engine thermal cycler (MJ Research).

    Techniques: Polymerase Chain Reaction

    The role of LEDGF/p75 DNA-binding in stimulating HIV-1 integration in vitro . ( A ) Efficient use of pTZ18U/PL as an integration target. Reactions in lanes 2, 4–8, 10 and 12–16 contained 0.6 µM integrase, whereas reactions in lanes 3–8 and 11–16 contained 0.2 µM of the indicated protein. Reactions in lanes 9–16 additionally contained pTZ18U/PL. The migration positions of mini-HIV substrate DNA, supercoiled (s.c.) pTZ18U/PL and strand transfer reaction products are indicated. The migration pattern of the plasmid on its own is shown on the right; molecular mass standards are to the left. ( B ) Integrase (IN) alone, integration reaction conducted with integrase alone. Other reactions additionally contained 0.2 µM of the indicated protein. Relative levels of integration activity were quantified by real-time PCR; IN alone activity was arbitrarily set to 1.0. Error bars are variation obtained from duplicate real-time assays. Similar levels of wild-type and mutant LEDGF/p75 activities were observed following independent sets of integration reactions.

    Journal: Nucleic Acids Research

    Article Title: A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo

    doi: 10.1093/nar/gkl052

    Figure Lengend Snippet: The role of LEDGF/p75 DNA-binding in stimulating HIV-1 integration in vitro . ( A ) Efficient use of pTZ18U/PL as an integration target. Reactions in lanes 2, 4–8, 10 and 12–16 contained 0.6 µM integrase, whereas reactions in lanes 3–8 and 11–16 contained 0.2 µM of the indicated protein. Reactions in lanes 9–16 additionally contained pTZ18U/PL. The migration positions of mini-HIV substrate DNA, supercoiled (s.c.) pTZ18U/PL and strand transfer reaction products are indicated. The migration pattern of the plasmid on its own is shown on the right; molecular mass standards are to the left. ( B ) Integrase (IN) alone, integration reaction conducted with integrase alone. Other reactions additionally contained 0.2 µM of the indicated protein. Relative levels of integration activity were quantified by real-time PCR; IN alone activity was arbitrarily set to 1.0. Error bars are variation obtained from duplicate real-time assays. Similar levels of wild-type and mutant LEDGF/p75 activities were observed following independent sets of integration reactions.

    Article Snippet: Reactions (30 µl) containing 1 µl of a 1:100 dilution of DNA, 0.3 µM of each primer and QuantiTect SYBR Green PCR Kit (QIAGEN) components were cycled in a DNA Engine Opticon thermal cycler (MJ Research Inc.) using 20 s of denaturation at 94°C, 20 s of annealing at 58°C and 45 s of extension at 72°C.

    Techniques: Binding Assay, In Vitro, Migration, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Mutagenesis

    PCR-based electrophoretic outlines of individuals acquired from three mollusk species. DNA isolated from Notoacmea concinna (lane 1~7), Sulculus diversicolor supertexta (lane 8~14) and Haliotis discus hannai (lane 15~21) were amplified by decamer primers BION-55 (A), BION-50 (B), BION-75 (C), BION-35 (D), BION-61 (E), BION-69 (F) and BION-66 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. Each lane shows DNA samples extracted and purified from 21 individuals. 100 bp ladder was used as a DNA molecular size marker (M).

    Journal: Development & Reproduction/Balsaeng'gwa saengsig

    Article Title: Genetic Distances of Three Mollusk Species Investigated by PCR Analysis

    doi: 10.12717/DR.2014.18.1.043

    Figure Lengend Snippet: PCR-based electrophoretic outlines of individuals acquired from three mollusk species. DNA isolated from Notoacmea concinna (lane 1~7), Sulculus diversicolor supertexta (lane 8~14) and Haliotis discus hannai (lane 15~21) were amplified by decamer primers BION-55 (A), BION-50 (B), BION-75 (C), BION-35 (D), BION-61 (E), BION-69 (F) and BION-66 (G). The PCR products were separated by 1.4% agarose gel electrophoresis and detected by ethidium bromide staining. Each lane shows DNA samples extracted and purified from 21 individuals. 100 bp ladder was used as a DNA molecular size marker (M).

    Article Snippet: PCR was performed using Programmable DNA Thermal Cycler (MJ Research Inc., Waltham, MA, USA).

    Techniques: Polymerase Chain Reaction, Isolation, Amplification, Agarose Gel Electrophoresis, Staining, Purification, Marker

    Detection sensitivity of multiplex-PCR-microarray for EVs. The detection sensitivity of the assay was estimated for EVs (EV71) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant EV71 control; B. The microarray result after amplification and hybridization. M: 1 Kb DNA ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant EV71 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.

    Journal: PLoS ONE

    Article Title: Evaluation of a Viral Microarray Based on Simultaneous Extraction and Amplification of Viral Nucleotide Acid for Detecting Human Herpesviruses and Enteroviruses

    doi: 10.1371/journal.pone.0117626

    Figure Lengend Snippet: Detection sensitivity of multiplex-PCR-microarray for EVs. The detection sensitivity of the assay was estimated for EVs (EV71) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant EV71 control; B. The microarray result after amplification and hybridization. M: 1 Kb DNA ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant EV71 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.

    Article Snippet: The PCR reaction was performed in a DNA Engine Tetrad Thermal Cycler (MJ Research, San Francisco, CA, USA) and initial denaturation was at 95°C for 5 min; and then 35 cycles at 95°C for 30 sec, 58°C for 30 sec, 72°C for 30 sec; a final extension at 72°C for 5 min.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Microarray, Reverse Transcription Polymerase Chain Reaction, Recombinant, Amplification, Hybridization

    Representative detection results from clinical samples. The DNA and RNA were extracted simultaneously from clinical samples and then amplified by use of the multiplex RT-PCR followed by hybridization with the microarray. The numbers of 1–8 showed the representative results from eight clinical samples. 1: HSV1; 2: HSV2; 3: EBV; 4: CMV; 5: EV71; 6: CA16; 7: CB5; 8: co-infection of EBV and CMV.

    Journal: PLoS ONE

    Article Title: Evaluation of a Viral Microarray Based on Simultaneous Extraction and Amplification of Viral Nucleotide Acid for Detecting Human Herpesviruses and Enteroviruses

    doi: 10.1371/journal.pone.0117626

    Figure Lengend Snippet: Representative detection results from clinical samples. The DNA and RNA were extracted simultaneously from clinical samples and then amplified by use of the multiplex RT-PCR followed by hybridization with the microarray. The numbers of 1–8 showed the representative results from eight clinical samples. 1: HSV1; 2: HSV2; 3: EBV; 4: CMV; 5: EV71; 6: CA16; 7: CB5; 8: co-infection of EBV and CMV.

    Article Snippet: The PCR reaction was performed in a DNA Engine Tetrad Thermal Cycler (MJ Research, San Francisco, CA, USA) and initial denaturation was at 95°C for 5 min; and then 35 cycles at 95°C for 30 sec, 58°C for 30 sec, 72°C for 30 sec; a final extension at 72°C for 5 min.

    Techniques: Amplification, Multiplex Assay, Reverse Transcription Polymerase Chain Reaction, Hybridization, Microarray, Infection

    Detection sensitivity of multiplex-PCR-microarray for HHVs. The detection sensitivity was estimated for HHVs (HSV1) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant HSV1 control; B. The microarray result after amplification and hybridization. M: 1 Kb DNA ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant HSV1 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.

    Journal: PLoS ONE

    Article Title: Evaluation of a Viral Microarray Based on Simultaneous Extraction and Amplification of Viral Nucleotide Acid for Detecting Human Herpesviruses and Enteroviruses

    doi: 10.1371/journal.pone.0117626

    Figure Lengend Snippet: Detection sensitivity of multiplex-PCR-microarray for HHVs. The detection sensitivity was estimated for HHVs (HSV1) by multiplex RT-PCR and microarray. A. Multiplex RT-PCR result from serial diluted recombinant HSV1 control; B. The microarray result after amplification and hybridization. M: 1 Kb DNA ladder; NTC: no template control. Numbers 1–10: Serial 10-fold dilutions of recombinant HSV1 control. 1: 6×10 8 copies/μl; 2: 6×10 7 copies/μl; 3: 6×10 6 copies/μl; 4: 6×10 5 copies/μl; 5: 6×10 4 copies/μl; 6: 6×10 3 copies/μl; 7: 6×10 2 copies/μl; 8: 6×10 1 copie/μl; 9: 6×10 0 copies/μl; 10: 6×10 –1 copies/μl.

    Article Snippet: The PCR reaction was performed in a DNA Engine Tetrad Thermal Cycler (MJ Research, San Francisco, CA, USA) and initial denaturation was at 95°C for 5 min; and then 35 cycles at 95°C for 30 sec, 58°C for 30 sec, 72°C for 30 sec; a final extension at 72°C for 5 min.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Microarray, Reverse Transcription Polymerase Chain Reaction, Recombinant, Amplification, Hybridization