dna substrates φx174 single-stranded ss virion dna Search Results


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  • 99
    New England Biolabs t4 polynucleotide kinase
    T4 Polynucleotide Kinase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 polynucleotide kinase/product/New England Biolabs
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    93
    New England Biolabs φx174 dna
    Substrate preference of wild-type EndA: Nucleolytic activity of wild-type EndA was tested on ( A ) <t>ΦX174</t> <t>DNA</t> (single-stranded, circular), ( B ) RF I DNA (double-stranded, circular) and ( C ) RF II DNA (double-stranded, nicked). Wild-type EndA shows no substrate preference (ss—single stranded, sc—super coiled, oc—open circle, li—linear).
    φx174 Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/φx174 dna/product/New England Biolabs
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    φx174 dna - by Bioz Stars, 2021-07
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    98
    Millipore atp regenerating system
    Substrate preference of wild-type EndA: Nucleolytic activity of wild-type EndA was tested on ( A ) <t>ΦX174</t> <t>DNA</t> (single-stranded, circular), ( B ) RF I DNA (double-stranded, circular) and ( C ) RF II DNA (double-stranded, nicked). Wild-type EndA shows no substrate preference (ss—single stranded, sc—super coiled, oc—open circle, li—linear).
    Atp Regenerating System, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC bacteriophage φx174
    A typical SPR reflectance angle minimum-time response used for the detection of bacteriophage (b. phage) <t>φX174</t> and other somatic coliphages present in the wastewater samples. Arrows indicate the different steps from the cycle that were described in Materials and Methods: (i) incubation of the host strain E. coli WG5, (ii) PBS washing, and (iii) addition of the sample bacteriophage in one chamber (and negative control in the other chamber).
    Bacteriophage φx174, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Substrate preference of wild-type EndA: Nucleolytic activity of wild-type EndA was tested on ( A ) ΦX174 DNA (single-stranded, circular), ( B ) RF I DNA (double-stranded, circular) and ( C ) RF II DNA (double-stranded, nicked). Wild-type EndA shows no substrate preference (ss—single stranded, sc—super coiled, oc—open circle, li—linear).

    Journal: Nucleic Acids Research

    Article Title: Mutational and biochemical analysis of the DNA-entry nuclease EndA from Streptococcus pneumoniae

    doi: 10.1093/nar/gkq802

    Figure Lengend Snippet: Substrate preference of wild-type EndA: Nucleolytic activity of wild-type EndA was tested on ( A ) ΦX174 DNA (single-stranded, circular), ( B ) RF I DNA (double-stranded, circular) and ( C ) RF II DNA (double-stranded, nicked). Wild-type EndA shows no substrate preference (ss—single stranded, sc—super coiled, oc—open circle, li—linear).

    Article Snippet: Cleavage preferences of EndA The wild-type EndA protein-synthesis reaction mixture was diluted 1:100 for cleavage of ΦX174 DNA (New England Biolabs): 8.4 nM single-stranded circular DNA 4.2 nM double-stranded, circular DNA and 4.2 nM double-stranded nicked DNA in 40 mM Tris–HCl, pH 7.7, 2 mM MgCl2 at ambient temperature.

    Techniques: Activity Assay

    A typical SPR reflectance angle minimum-time response used for the detection of bacteriophage (b. phage) φX174 and other somatic coliphages present in the wastewater samples. Arrows indicate the different steps from the cycle that were described in Materials and Methods: (i) incubation of the host strain E. coli WG5, (ii) PBS washing, and (iii) addition of the sample bacteriophage in one chamber (and negative control in the other chamber).

    Journal: Applied and Environmental Microbiology

    Article Title: Surface Plasmon Resonance Assay for Real-Time Monitoring of Somatic Coliphages in Wastewaters

    doi: 10.1128/AEM.02806-07

    Figure Lengend Snippet: A typical SPR reflectance angle minimum-time response used for the detection of bacteriophage (b. phage) φX174 and other somatic coliphages present in the wastewater samples. Arrows indicate the different steps from the cycle that were described in Materials and Methods: (i) incubation of the host strain E. coli WG5, (ii) PBS washing, and (iii) addition of the sample bacteriophage in one chamber (and negative control in the other chamber).

    Article Snippet: Bacteriophage φX174 (ATCC 13706-B1) was used as a reference bacteriophage for somatic coliphages.

    Techniques: SPR Assay, Incubation, Negative Control

    Variation of the reflectance angle minimum in response time detection with different concentrations of bacteriophages. (a) Detection of various concentrations of bacteriophage φX174 as measured by the SPR minimum angle change with time. Threshold time for 10 8 PFU/ml φX174 is marked on the plot for illustration. (b) Mean values of SPR minimum angle with time for detection of somatic coliphages in wastewater samples. Error bars show standard errors for triplicate repeats.

    Journal: Applied and Environmental Microbiology

    Article Title: Surface Plasmon Resonance Assay for Real-Time Monitoring of Somatic Coliphages in Wastewaters

    doi: 10.1128/AEM.02806-07

    Figure Lengend Snippet: Variation of the reflectance angle minimum in response time detection with different concentrations of bacteriophages. (a) Detection of various concentrations of bacteriophage φX174 as measured by the SPR minimum angle change with time. Threshold time for 10 8 PFU/ml φX174 is marked on the plot for illustration. (b) Mean values of SPR minimum angle with time for detection of somatic coliphages in wastewater samples. Error bars show standard errors for triplicate repeats.

    Article Snippet: Bacteriophage φX174 (ATCC 13706-B1) was used as a reference bacteriophage for somatic coliphages.

    Techniques: SPR Assay