dna sequencing reactions Search Results


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  • 98
    Thermo Fisher dna sequencing analysis software
    Dna Sequencing Analysis Software, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna sequencing analysis software/product/Thermo Fisher
    Average 98 stars, based on 128 article reviews
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    99
    Millipore dna sequence analysis dna sequence analyses
    Identification of a Thr54Met <t>GNA11</t> mutation in a FHH proband. ( A ) <t>DNA</t> sequence analyses revealed a heterozygous C‐to‐T transition at nucleotide c.161 (red arrow) within exon 2 of GNA11 . ( B ) This sequence abnormality was predicted to lead to a missense amino acid substitution of Thr to Met at codon 54, resulting in the loss of a BsiHKAI restriction endonuclease site (GAGCA/C) and gain of an NspI (GCATG/T) restriction endonuclease site. ( C ) Restriction maps showing that BsiHKAI digestion would result in two products of 104 bp and 293 bp from the wild‐type (WT) sequence but not the mutant (m) sequence. In contrast, NspI digestion results in two products of 104 bp and 293 bp from the m sequence but not the WT sequence. ( D ) Restriction endonuclease (RE) digest of PCR products of exon 2 of GNA11 demonstrating the heterozygous C‐to‐T transition and confirming its absence in two unrelated unaffected individuals (N1 and N2). S = size marker. For BsiHKAI RE digest the mutant product (397 bp) and WT product (293 bp) are shown; the WT product at 104 bp is not shown. For NspI RE digest the WT product (397 bp) and mutant product (293 bp) are shown; the mutant product at 104 bp is not shown.
    Dna Sequence Analysis Dna Sequence Analyses, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    MWG-Biotech dna sequencing analysis
    Identification of a Thr54Met <t>GNA11</t> mutation in a FHH proband. ( A ) <t>DNA</t> sequence analyses revealed a heterozygous C‐to‐T transition at nucleotide c.161 (red arrow) within exon 2 of GNA11 . ( B ) This sequence abnormality was predicted to lead to a missense amino acid substitution of Thr to Met at codon 54, resulting in the loss of a BsiHKAI restriction endonuclease site (GAGCA/C) and gain of an NspI (GCATG/T) restriction endonuclease site. ( C ) Restriction maps showing that BsiHKAI digestion would result in two products of 104 bp and 293 bp from the wild‐type (WT) sequence but not the mutant (m) sequence. In contrast, NspI digestion results in two products of 104 bp and 293 bp from the m sequence but not the WT sequence. ( D ) Restriction endonuclease (RE) digest of PCR products of exon 2 of GNA11 demonstrating the heterozygous C‐to‐T transition and confirming its absence in two unrelated unaffected individuals (N1 and N2). S = size marker. For BsiHKAI RE digest the mutant product (397 bp) and WT product (293 bp) are shown; the WT product at 104 bp is not shown. For NspI RE digest the WT product (397 bp) and mutant product (293 bp) are shown; the mutant product at 104 bp is not shown.
    Dna Sequencing Analysis, supplied by MWG-Biotech, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Elim Bio dna sequence analysis
    Determination of nucleotides at the 5′ and 3′ ends of <t>HCV</t> replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by <t>DNA</t> sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.
    Dna Sequence Analysis, supplied by Elim Bio, used in various techniques. Bioz Stars score: 92/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Gene Codes Inc sequencher dna sequence analysis software v2 1
    Determination of nucleotides at the 5′ and 3′ ends of <t>HCV</t> replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by <t>DNA</t> sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.
    Sequencher Dna Sequence Analysis Software V2 1, supplied by Gene Codes Inc, used in various techniques. Bioz Stars score: 86/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 28 article reviews
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    88
    Gene Codes Inc sequencher dna sequence analysis software version 4 2
    Determination of nucleotides at the 5′ and 3′ ends of <t>HCV</t> replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by <t>DNA</t> sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.
    Sequencher Dna Sequence Analysis Software Version 4 2, supplied by Gene Codes Inc, used in various techniques. Bioz Stars score: 88/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Sangon Biotech dna sequence analysis
    Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the <t>LAMP</t> method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid <t>DNA);</t> 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.
    Dna Sequence Analysis, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 91/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Shanghai GenePharma dna sequencing analysis
    Hep G2 cells with integrated HPV 18 <t>DNA</t> expressed E6 and <t>E7</t> mRNAs and proteins. (A) PCR amplification of HPV 18 E6E7 gene was assessed in samples from Hep G2, EC109, and K562 cells. Then an amplified fragment of 847 bp was present in both Hep G2 and EC109 cells (HPV 18 positive), but absent in K562 cells (HPV 18 negative). (B) The expression of HPV 18 E6 and E7 mRNA was detected by RT-PCR. The expected fragments of E6 (196 bp) and E7 (332 bp) were present in both Hep G2 and EC109 cells, but not in K562 cells. (C) Western blotting showed the expression of the HPV 18 E6 and E7 proteins. EC109 and K562 were used as controls. β-actin was used as an internal control.
    Dna Sequencing Analysis, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Macrogen dna sequencing analyses
    Hep G2 cells with integrated HPV 18 <t>DNA</t> expressed E6 and <t>E7</t> mRNAs and proteins. (A) PCR amplification of HPV 18 E6E7 gene was assessed in samples from Hep G2, EC109, and K562 cells. Then an amplified fragment of 847 bp was present in both Hep G2 and EC109 cells (HPV 18 positive), but absent in K562 cells (HPV 18 negative). (B) The expression of HPV 18 E6 and E7 mRNA was detected by RT-PCR. The expected fragments of E6 (196 bp) and E7 (332 bp) were present in both Hep G2 and EC109 cells, but not in K562 cells. (C) Western blotting showed the expression of the HPV 18 E6 and E7 proteins. EC109 and K562 were used as controls. β-actin was used as an internal control.
    Dna Sequencing Analyses, supplied by Macrogen, used in various techniques. Bioz Stars score: 88/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher dna sequence analysis
    Detection of the mRNA expression levels of IgG and related enzymes, including RAG1, RAG2 and AID in podocytes. (A) Transcripts of Igγ, γ1, γ3, γ4, κ and λ C regions were detected by <t>RT-PCR.</t> CD19 was detected as a marker of B lymphocytes. The mRNA of Igγ and κ V regions was amplified by nested RT-PCR. (B) mRNA expression levels of AID, RAG1 and RAG2. PBMCs were used as a positive control; water instead of cDNA was used as a blank control; R (cDNA template replaced by DNase-treated RNA) was used as negative control. (C) <t>DNA</t> sequencing results. The sequences of the PCR products were aligned with the mRNA sequences available in the NCBI database. HPC, human podocytes; AID, activation-induced cytidine deaminase; C, constant; Ig, immunoglobulin; Igγ, IgG heavy chain; PBMCs, peripheral blood mononuclear cells; RAG. recombination activating gene; RT-PCR, reverse transcription-polymerase chain reaction; V, variable.
    Dna Sequence Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Beckman Coulter dna sequence analysis
    Detection of the mRNA expression levels of IgG and related enzymes, including RAG1, RAG2 and AID in podocytes. (A) Transcripts of Igγ, γ1, γ3, γ4, κ and λ C regions were detected by <t>RT-PCR.</t> CD19 was detected as a marker of B lymphocytes. The mRNA of Igγ and κ V regions was amplified by nested RT-PCR. (B) mRNA expression levels of AID, RAG1 and RAG2. PBMCs were used as a positive control; water instead of cDNA was used as a blank control; R (cDNA template replaced by DNase-treated RNA) was used as negative control. (C) <t>DNA</t> sequencing results. The sequences of the PCR products were aligned with the mRNA sequences available in the NCBI database. HPC, human podocytes; AID, activation-induced cytidine deaminase; C, constant; Ig, immunoglobulin; Igγ, IgG heavy chain; PBMCs, peripheral blood mononuclear cells; RAG. recombination activating gene; RT-PCR, reverse transcription-polymerase chain reaction; V, variable.
    Dna Sequence Analysis, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bioneer Corporation dna sequencing analysis
    Agarose gel electrophoresis of <t>PCR</t> assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp <t>DNA</t> marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.
    Dna Sequencing Analysis, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    SeqWright dna sequence analyses
    Agarose gel electrophoresis of <t>PCR</t> assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp <t>DNA</t> marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.
    Dna Sequence Analyses, supplied by SeqWright, used in various techniques. Bioz Stars score: 89/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche dna sequencing sequence analysis
    Agarose gel electrophoresis of <t>PCR</t> assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp <t>DNA</t> marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.
    Dna Sequencing Sequence Analysis, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher abi 3730 xl dna sequencing analyser
    Agarose gel electrophoresis of <t>PCR</t> assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp <t>DNA</t> marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.
    Abi 3730 Xl Dna Sequencing Analyser, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Lonestar Heart dna sequence analysis
    Agarose gel electrophoresis of <t>PCR</t> assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp <t>DNA</t> marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.
    Dna Sequence Analysis, supplied by Lonestar Heart, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Eurofins dna sequencing analysis
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    Dna Sequencing Analysis, supplied by Eurofins, used in various techniques. Bioz Stars score: 91/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dna sequence analyser abi3720
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    Dna Sequence Analyser Abi3720, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genomed GmbH dna sequence analysis
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    Dna Sequence Analysis, supplied by Genomed GmbH, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher 373a automatic dna sequence analysis machine
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    373a Automatic Dna Sequence Analysis Machine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher abi prism 3130 dna analyser sequencer
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    Abi Prism 3130 Dna Analyser Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher dna sequencing analysis 3 7
    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct <t>PCR</t> with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by <t>DNA</t> sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown
    Dna Sequencing Analysis 3 7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Identification of a Thr54Met GNA11 mutation in a FHH proband. ( A ) DNA sequence analyses revealed a heterozygous C‐to‐T transition at nucleotide c.161 (red arrow) within exon 2 of GNA11 . ( B ) This sequence abnormality was predicted to lead to a missense amino acid substitution of Thr to Met at codon 54, resulting in the loss of a BsiHKAI restriction endonuclease site (GAGCA/C) and gain of an NspI (GCATG/T) restriction endonuclease site. ( C ) Restriction maps showing that BsiHKAI digestion would result in two products of 104 bp and 293 bp from the wild‐type (WT) sequence but not the mutant (m) sequence. In contrast, NspI digestion results in two products of 104 bp and 293 bp from the m sequence but not the WT sequence. ( D ) Restriction endonuclease (RE) digest of PCR products of exon 2 of GNA11 demonstrating the heterozygous C‐to‐T transition and confirming its absence in two unrelated unaffected individuals (N1 and N2). S = size marker. For BsiHKAI RE digest the mutant product (397 bp) and WT product (293 bp) are shown; the WT product at 104 bp is not shown. For NspI RE digest the WT product (397 bp) and mutant product (293 bp) are shown; the mutant product at 104 bp is not shown.

    Journal: Journal of Bone and Mineral Research

    Article Title: A G‐protein Subunit‐α11 Loss‐of‐Function Mutation, Thr54Met, Causes Familial Hypocalciuric Hypercalcemia Type 2 (FHH2)

    doi: 10.1002/jbmr.2778

    Figure Lengend Snippet: Identification of a Thr54Met GNA11 mutation in a FHH proband. ( A ) DNA sequence analyses revealed a heterozygous C‐to‐T transition at nucleotide c.161 (red arrow) within exon 2 of GNA11 . ( B ) This sequence abnormality was predicted to lead to a missense amino acid substitution of Thr to Met at codon 54, resulting in the loss of a BsiHKAI restriction endonuclease site (GAGCA/C) and gain of an NspI (GCATG/T) restriction endonuclease site. ( C ) Restriction maps showing that BsiHKAI digestion would result in two products of 104 bp and 293 bp from the wild‐type (WT) sequence but not the mutant (m) sequence. In contrast, NspI digestion results in two products of 104 bp and 293 bp from the m sequence but not the WT sequence. ( D ) Restriction endonuclease (RE) digest of PCR products of exon 2 of GNA11 demonstrating the heterozygous C‐to‐T transition and confirming its absence in two unrelated unaffected individuals (N1 and N2). S = size marker. For BsiHKAI RE digest the mutant product (397 bp) and WT product (293 bp) are shown; the WT product at 104 bp is not shown. For NspI RE digest the WT product (397 bp) and mutant product (293 bp) are shown; the mutant product at 104 bp is not shown.

    Article Snippet: DNA sequence analysis DNA sequence analyses of GNA11 exons 1–7 and their adjacent splice sites (NM_002067) (Fig. ) was performed using leucocyte DNA and gene‐specific primers (Sigma‐Aldrich, St. Louis, MO, USA), as previously reported.

    Techniques: Mutagenesis, Sequencing, Polymerase Chain Reaction, Marker

    Determination of nucleotides at the 5′ and 3′ ends of HCV replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by DNA sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.

    Journal: Journal of Virology

    Article Title: Effects of Mutations of the Initiation Nucleotides on Hepatitis C Virus RNA Replication in the Cell

    doi: 10.1128/JVI.78.7.3633-3643.2004

    Figure Lengend Snippet: Determination of nucleotides at the 5′ and 3′ ends of HCV replicon RNAs recovered from G418-resistant Huh7 cells. (A) Schematic of the RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) (Ambion). An adapter RNA (open bar) was ligated to the 5′ end of either the positive-strand (for determination of the 5′-end nucleotide) or the negative-strand (for determination of the 3′-end nucleotide) HCV replicon RNA isolated from different cell lines. The cDNAs of both positive and negative strands of HCV RNA were reverse transcribed by using HCV-specific primers and amplified by PCR with 5′RACE outer/inner primers (gray arrow) and HCV-specific primers (open arrow). The 5′-end nucleotides of both positive and negative strands of HCV RNA were determined by DNA sequence analysis. (B) 5′- and 3′-end nucleotides of HCV RNA determined by RLM-RACE. Letters indicate nucleotides at the 5′ and 3′ ends as shown on the top. The number indicates the number of cell clones from which HCV replicon RNAs were analyzed. Numbers in parentheses indicate the frequency of the nucleotides determined. The HCV replicon RNAs given on the left column were the ones transcribed in vitro by a T7 RNA polymerase.

    Article Snippet: Further analyses of HCV RNA derived from this patient by cDNA cloning and DNA sequence analysis revealed that the mixture of HCV RNA consists of 60% of RNA with a 5′-end nucleotide G and 40% of RNA with a 5′-end nucleotide A.

    Techniques: Rapid Amplification of cDNA Ends, Isolation, Amplification, Polymerase Chain Reaction, Sequencing, Clone Assay, In Vitro

    Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the LAMP method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid DNA); 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.

    Journal: Tropical Medicine and Infectious Disease

    Article Title: Field Evaluation of a Loop-Mediated Isothermal Amplification (LAMP) Platform for the Detection of Schistosoma japonicum Infection in Oncomelania hupensis Snails

    doi: 10.3390/tropicalmed3040124

    Figure Lengend Snippet: Detection of the Sj28S gene component in the different parasite developmental stages of S. japonicum in O. hupensis by the LAMP method. 1. Negative control (nuclease-free water); 2. Positive control (Sj28 plasmid DNA); 3. Cercariae; 4. Specificity control ( Trichobilharzia cercariae); 5. Negative control (pooled snail DNA from a non-endemic area); 6. Mother sporocyst; 7. Daughter sporocyst.

    Article Snippet: Out of the 232 pooled snail samples tested, 217 were found to be negative and 14 positive by both assays , while the remaining single sample, found positive by LAMP but negative by PCR, was found to be positive by DNA sequence analysis, underlining LAMP’s superiority.

    Techniques: Negative Control, Positive Control, Plasmid Preparation

    Hep G2 cells with integrated HPV 18 DNA expressed E6 and E7 mRNAs and proteins. (A) PCR amplification of HPV 18 E6E7 gene was assessed in samples from Hep G2, EC109, and K562 cells. Then an amplified fragment of 847 bp was present in both Hep G2 and EC109 cells (HPV 18 positive), but absent in K562 cells (HPV 18 negative). (B) The expression of HPV 18 E6 and E7 mRNA was detected by RT-PCR. The expected fragments of E6 (196 bp) and E7 (332 bp) were present in both Hep G2 and EC109 cells, but not in K562 cells. (C) Western blotting showed the expression of the HPV 18 E6 and E7 proteins. EC109 and K562 were used as controls. β-actin was used as an internal control.

    Journal: PLoS ONE

    Article Title: Human Papillomavirus Type 18 E6 and E7 Genes Integrate into Human Hepatoma Derived Cell Line Hep G2

    doi: 10.1371/journal.pone.0037964

    Figure Lengend Snippet: Hep G2 cells with integrated HPV 18 DNA expressed E6 and E7 mRNAs and proteins. (A) PCR amplification of HPV 18 E6E7 gene was assessed in samples from Hep G2, EC109, and K562 cells. Then an amplified fragment of 847 bp was present in both Hep G2 and EC109 cells (HPV 18 positive), but absent in K562 cells (HPV 18 negative). (B) The expression of HPV 18 E6 and E7 mRNA was detected by RT-PCR. The expected fragments of E6 (196 bp) and E7 (332 bp) were present in both Hep G2 and EC109 cells, but not in K562 cells. (C) Western blotting showed the expression of the HPV 18 E6 and E7 proteins. EC109 and K562 were used as controls. β-actin was used as an internal control.

    Article Snippet: The siRNA E7-63 was separately cloned into pGPH1 vector and this was verified through DNA sequencing analysis performed at Shanghai GenePharma Company.

    Techniques: Polymerase Chain Reaction, Amplification, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Detection of the mRNA expression levels of IgG and related enzymes, including RAG1, RAG2 and AID in podocytes. (A) Transcripts of Igγ, γ1, γ3, γ4, κ and λ C regions were detected by RT-PCR. CD19 was detected as a marker of B lymphocytes. The mRNA of Igγ and κ V regions was amplified by nested RT-PCR. (B) mRNA expression levels of AID, RAG1 and RAG2. PBMCs were used as a positive control; water instead of cDNA was used as a blank control; R (cDNA template replaced by DNase-treated RNA) was used as negative control. (C) DNA sequencing results. The sequences of the PCR products were aligned with the mRNA sequences available in the NCBI database. HPC, human podocytes; AID, activation-induced cytidine deaminase; C, constant; Ig, immunoglobulin; Igγ, IgG heavy chain; PBMCs, peripheral blood mononuclear cells; RAG. recombination activating gene; RT-PCR, reverse transcription-polymerase chain reaction; V, variable.

    Journal: International Journal of Molecular Medicine

    Article Title: Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion

    doi: 10.3892/ijmm.2018.3525

    Figure Lengend Snippet: Detection of the mRNA expression levels of IgG and related enzymes, including RAG1, RAG2 and AID in podocytes. (A) Transcripts of Igγ, γ1, γ3, γ4, κ and λ C regions were detected by RT-PCR. CD19 was detected as a marker of B lymphocytes. The mRNA of Igγ and κ V regions was amplified by nested RT-PCR. (B) mRNA expression levels of AID, RAG1 and RAG2. PBMCs were used as a positive control; water instead of cDNA was used as a blank control; R (cDNA template replaced by DNase-treated RNA) was used as negative control. (C) DNA sequencing results. The sequences of the PCR products were aligned with the mRNA sequences available in the NCBI database. HPC, human podocytes; AID, activation-induced cytidine deaminase; C, constant; Ig, immunoglobulin; Igγ, IgG heavy chain; PBMCs, peripheral blood mononuclear cells; RAG. recombination activating gene; RT-PCR, reverse transcription-polymerase chain reaction; V, variable.

    Article Snippet: The reliability of PCR products was analyzed by DNA sequence analysis, which was performed by Invitrogen Trading (Shanghai) Co., Ltd. (Shanghai, China).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Marker, Amplification, Positive Control, Negative Control, DNA Sequencing, Polymerase Chain Reaction, Activation Assay

    Agarose gel electrophoresis of PCR assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp DNA marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.

    Journal: Iranian Journal of Medical Sciences

    Article Title: Isolation and Detection of Erysipelothrix rhusiopathiae and Its Distribution in Humans and Animals by Phenotypical and Molecular Methods in Ahvaz-Iran in 2015

    doi:

    Figure Lengend Snippet: Agarose gel electrophoresis of PCR assay for the identification of Erysipelothrix rhusiopathiae. Lane 1: 100bp DNA marker; Lanes 2–8 and 11: positive samples; Lanes 10: negative control; Lane 12: Erysipelothrix rhusiopathiae ATCC 19414 as positive control.

    Article Snippet: DNA Sequencing Analysis All the PCR products (20 cases) were sequenced at Bioneer Company (Korea), and all the sequences were compared with GenBank.

    Techniques: Agarose Gel Electrophoresis, Polymerase Chain Reaction, Marker, Negative Control, Positive Control

    Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct PCR with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by DNA sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown

    Journal: Microbial Cell Factories

    Article Title: Oral delivery of Lactococcus lactis that secretes bioactive heme oxygenase-1 alleviates development of acute colitis in mice

    doi: 10.1186/s12934-015-0378-2

    Figure Lengend Snippet: Survival of NZ-HO in the mouse intestine. a Experimental schedule. b Homogenates of the entire colon (including luminal contents) were plated on GM17 cm agar. c , d Ten single colonies were randomly chosen for each sample from healthy ( c ) or colitis ( d ) mice and were subjected to colony-direct PCR with pNZ8148-specific ( upper images of each group) or L. lactis subsp. cremoris -specific ( lower images of each group) primer pairs. Bands indicated by blue (586 bp), salmon pink (1,403 bp), and black arrows (163 bp) were further analyzed by DNA sequencing and were consistent with the putative sequences. L : DNA ladder (bp). e Immunohistochemical staining/detection of His-tagged proteins in colonic tissue. Positive reactions were observed in the mucosal epithelial cells ( arrows ), in the crypt, and the lamina propria ( asterisks ) of the colon from gmNZ9000-treated mice. Bars 50 μm. Normal colon and Inflamed colon mean colons from healthy (mice that drank sterile water) or colitis (mice that drank DSS in the water) mice, respectively. Similar results were seen in two different mice. Representative images are shown

    Article Snippet: DNA sequences of PCR products were determined by DNA sequencing analysis, which was performed by Eurofins Genomics.

    Techniques: Mouse Assay, Polymerase Chain Reaction, DNA Sequencing, Immunohistochemistry, Staining