dna seq id Search Results


dna smart chip seq kit  (TaKaRa)
94
TaKaRa dna smart chip seq kit
HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, <t>DNA</t> ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and <t>sequencing</t> <t>(ChIP-seq)</t> with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.
Dna Smart Chip Seq Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dna smart chip seq kit - by Bioz Stars, 2025-07
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thruplex dna seq kit  (TaKaRa)
97
TaKaRa thruplex dna seq kit
HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, <t>DNA</t> ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and <t>sequencing</t> <t>(ChIP-seq)</t> with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.
Thruplex Dna Seq Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thruplex dna seq kit/product/TaKaRa
Average 97 stars, based on 1 article reviews
thruplex dna seq kit - by Bioz Stars, 2025-07
97/100 stars
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pcr primers n a n a fluidigm access array barcodes fluidigm  (fluidigm)
91
fluidigm pcr primers n a n a fluidigm access array barcodes fluidigm
HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, <t>DNA</t> ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and <t>sequencing</t> <t>(ChIP-seq)</t> with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.
Pcr Primers N A N A Fluidigm Access Array Barcodes Fluidigm, supplied by fluidigm, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr primers n a n a fluidigm access array barcodes fluidigm/product/fluidigm
Average 91 stars, based on 1 article reviews
pcr primers n a n a fluidigm access array barcodes fluidigm - by Bioz Stars, 2025-07
91/100 stars
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dna seq  (fluidigm)
91
fluidigm dna seq
HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, <t>DNA</t> ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and <t>sequencing</t> <t>(ChIP-seq)</t> with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.
Dna Seq, supplied by fluidigm, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna seq/product/fluidigm
Average 91 stars, based on 1 article reviews
dna seq - by Bioz Stars, 2025-07
91/100 stars
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Image Search Results


HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, DNA ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and sequencing (ChIP-seq) with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.

Journal: Methods in enzymology

Article Title: Genomic mapping of DNA-repair reaction intermediates in living cells with engineered DNA structure-trap proteins

doi: 10.1016/bs.mie.2021.09.015

Figure Lengend Snippet: HJ mapping by X-seq in E. coli. (A) Diagram of X-seq (Mei et al., 2021; Xia et al., 2016). RuvCDefGFP-bound HJs are immunoprecipitated with anti-RuvC antibody. While RuvCDefGFP remains bound to immobilized antibody-protein A complex, DNA ends are blunted, A-tailed, and ligated with NEBNext adapters prior to elution. Adapter-ligated DNA fragments are converted into a sequencing library by PCR amplification, then sequenced. (B) X-seq detects three significant spontaneous HJ sites within the terminus region of the E. coli genome, one at the TerA unidirectional replication-barrier site, and two that flank the dif site of chromosome decatenation. All three form dependently on DSB repair-proteins RecA and RecBCD indicating their origins in DSB repair (see Fig. 1B). Replication terminus region at top, and origin, oriC, at the bottom. Orange, X-seq with RuvC antibody; sky blue, chromatin immunoprecipitation and sequencing (ChIP-seq) with nonspecific immunoglobulin G2a (IgG2a). Italics capital letters A–H, locations and orientations of TerA-TerH sites. Panel (B): Data from Mei, Q., Fitzgerald, D. M., Liu, J., Xia, J., Pribis, J. P., Zhai, Y., et al. (2021). Two mechanisms of chromosome fragility at replication-termination sites in bacteria. Science Advances, 7(25), eabe2846.

Article Snippet: 3 Prepare library with the Takara DNA SMART ChIP-Seq Kit according to its user manual.

Techniques: Immunoprecipitation, Sequencing, Amplification, Chromatin Immunoprecipitation, ChIP-sequencing

SsEND-seq mapping of 3ʹ-ssDNA-ends in E. coli. (A) Diagram of SsEND-seq. ExID-FLAG-bound 3ʹ-ssDNA ends are immunoprecipitated with anti-FLAG antibody, followed by strand-specific library preparation using the Takara DNA SMART ChIP-Seq Kit, in which 5ʹ and 3ʹ ends of ssDNA fragments are tagged with known adapter sequences, then sequenced and a genomic map of recurrent 3ʹ-ssDNA ends is made. (B) SsEND-seq detects asymmetrical peaks. SsEND-seq reads are mapped to the top (TOP, orange) or bottom (BTM, purple) strand, and form different asymmetrical peaks in which summits indicate recurrent DNA 3ʹ ssDNA termini that ExID traps. (C) SsEND-seq detects peaks of predicted asymmetry and strandedness (Liu et al. (n.d.)). Heatmaps of SsEND-seq signals around peak summits of ~220 recurrent 3ʹ-ssDNA end sites. The signals were normalized by Reads Per Kilobase per Million mapped reads (RPKM), and sorted according to signal intensity. Signals are displayed along ±300-nt regions around and centered along the peak summits.

Journal: Methods in enzymology

Article Title: Genomic mapping of DNA-repair reaction intermediates in living cells with engineered DNA structure-trap proteins

doi: 10.1016/bs.mie.2021.09.015

Figure Lengend Snippet: SsEND-seq mapping of 3ʹ-ssDNA-ends in E. coli. (A) Diagram of SsEND-seq. ExID-FLAG-bound 3ʹ-ssDNA ends are immunoprecipitated with anti-FLAG antibody, followed by strand-specific library preparation using the Takara DNA SMART ChIP-Seq Kit, in which 5ʹ and 3ʹ ends of ssDNA fragments are tagged with known adapter sequences, then sequenced and a genomic map of recurrent 3ʹ-ssDNA ends is made. (B) SsEND-seq detects asymmetrical peaks. SsEND-seq reads are mapped to the top (TOP, orange) or bottom (BTM, purple) strand, and form different asymmetrical peaks in which summits indicate recurrent DNA 3ʹ ssDNA termini that ExID traps. (C) SsEND-seq detects peaks of predicted asymmetry and strandedness (Liu et al. (n.d.)). Heatmaps of SsEND-seq signals around peak summits of ~220 recurrent 3ʹ-ssDNA end sites. The signals were normalized by Reads Per Kilobase per Million mapped reads (RPKM), and sorted according to signal intensity. Signals are displayed along ±300-nt regions around and centered along the peak summits.

Article Snippet: 3 Prepare library with the Takara DNA SMART ChIP-Seq Kit according to its user manual.

Techniques: Immunoprecipitation, ChIP-sequencing