dna polymerase Stratagene Search Results


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  • 78
    Stratagene herculase dnapol
    Translation of a <t>DNA</t> template into PNA and displacement of the resulting PNA strand Following PNA polymerization (lane 5) under conditions described in Figure 2 , the PNA strand is displaced by primer extension of the 3′ hairpin using <t>Herculase</t> DNA polymerase II and dNTPs (lane 6). DNA polymerization to the end of the template strand (through the 5′ stem-loop) generates an 84-base pair DNA duplex that can form as a mutually exclusive alternative to the 40-base pair PNA-DNA heteroduplex and 24-base pair DNA-DNA stem. Successful strand displacement creates a double-stranded Bcc I restriction endonuclease cleavage site. The Bcc I-mediated cleavage of the primer extension product (lanes 4 and 8) is therefore consistent with displacement of the PNA strand. The red dot represents a Cy3 backbone spacer that blocks subsequentual DNA polymerization. The green dot represents a fluorescein group. The denaturing PAGE gel shown is visualized by imaging fluorescein fluorescence. Reactions shown in lanes labeled “translation–” lacked NaCNBH 3 ; those in lanes labeled “displacement–” lacked DNA polymerase; those in lanes labeled “restriction digestion–” lacked Bcc I.
    Herculase Dnapol, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/herculase dnapol/product/Stratagene
    Average 78 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    herculase dnapol - by Bioz Stars, 2020-02
    78/100 stars
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    80
    Stratagene pfuturbo stratagene dna polymerase
    Quantitative assessment of viral <t>DNA</t> in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 <t>pfu</t> of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.
    Pfuturbo Stratagene Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbo stratagene dna polymerase/product/Stratagene
    Average 80 stars, based on 646 article reviews
    Price from $9.99 to $1999.99
    pfuturbo stratagene dna polymerase - by Bioz Stars, 2020-02
    80/100 stars
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    81
    Stratagene pfu stratagene dna polymerase
    Quantitative assessment of viral <t>DNA</t> in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 <t>pfu</t> of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.
    Pfu Stratagene Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 81/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 81 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    pfu stratagene dna polymerase - by Bioz Stars, 2020-02
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    91
    Stratagene dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 670 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 670 article reviews
    Price from $9.99 to $1999.99
    dna polymerase - by Bioz Stars, 2020-02
    91/100 stars
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    99
    Stratagene pfuultra dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Pfuultra Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 99/100, based on 568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuultra dna polymerase/product/Stratagene
    Average 99 stars, based on 568 article reviews
    Price from $9.99 to $1999.99
    pfuultra dna polymerase - by Bioz Stars, 2020-02
    99/100 stars
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    83
    Stratagene dna polymerase pfuturbor
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Dna Polymerase Pfuturbor, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 83 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    dna polymerase pfuturbor - by Bioz Stars, 2020-02
    83/100 stars
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    83
    Stratagene easya dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Easya Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/easya dna polymerase/product/Stratagene
    Average 83 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    easya dna polymerase - by Bioz Stars, 2020-02
    83/100 stars
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    89
    Stratagene exl dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Exl Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 62 article reviews
    Price from $9.99 to $1999.99
    exl dna polymerase - by Bioz Stars, 2020-02
    89/100 stars
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    75
    Stratagene paq5000 dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Paq5000 Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 75/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paq5000 dna polymerase/product/Stratagene
    Average 75 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    paq5000 dna polymerase - by Bioz Stars, 2020-02
    75/100 stars
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    80
    Stratagene thermostable dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Thermostable Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thermostable dna polymerase/product/Stratagene
    Average 80 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    thermostable dna polymerase - by Bioz Stars, 2020-02
    80/100 stars
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    87
    Stratagene paq dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Paq Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 87/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paq dna polymerase/product/Stratagene
    Average 87 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    paq dna polymerase - by Bioz Stars, 2020-02
    87/100 stars
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    81
    Stratagene pfuturbotm dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Pfuturbotm Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuturbotm dna polymerase/product/Stratagene
    Average 81 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pfuturbotm dna polymerase - by Bioz Stars, 2020-02
    81/100 stars
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    78
    Stratagene pfuultratm dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Pfuultratm Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pfuultratm dna polymerase/product/Stratagene
    Average 78 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    pfuultratm dna polymerase - by Bioz Stars, 2020-02
    78/100 stars
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    84
    Stratagene turbopfu dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Turbopfu Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 84/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turbopfu dna polymerase/product/Stratagene
    Average 84 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    turbopfu dna polymerase - by Bioz Stars, 2020-02
    84/100 stars
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    80
    Stratagene yieldace dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Yieldace Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yieldace dna polymerase/product/Stratagene
    Average 80 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    yieldace dna polymerase - by Bioz Stars, 2020-02
    80/100 stars
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    87
    Stratagene turbo dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Turbo Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 87/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/turbo dna polymerase/product/Stratagene
    Average 87 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    turbo dna polymerase - by Bioz Stars, 2020-02
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    87
    Stratagene taq2000 dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Taq2000 Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 87/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 87 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    taq2000 dna polymerase - by Bioz Stars, 2020-02
    87/100 stars
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    79
    Stratagene pfuultraii dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Pfuultraii Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    pfuultraii dna polymerase - by Bioz Stars, 2020-02
    79/100 stars
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    82
    Stratagene picomaxx dna polymerase
    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a <t>PCR</t> at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate <t>DNA</t> bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.
    Picomaxx Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 82/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/picomaxx dna polymerase/product/Stratagene
    Average 82 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    picomaxx dna polymerase - by Bioz Stars, 2020-02
    82/100 stars
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    79
    Stratagene native dna polymerase
    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
    Native Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with <t>HCMV,</t> incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV <t>DNA</t> per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.
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    Image Search Results


    Translation of a DNA template into PNA and displacement of the resulting PNA strand Following PNA polymerization (lane 5) under conditions described in Figure 2 , the PNA strand is displaced by primer extension of the 3′ hairpin using Herculase DNA polymerase II and dNTPs (lane 6). DNA polymerization to the end of the template strand (through the 5′ stem-loop) generates an 84-base pair DNA duplex that can form as a mutually exclusive alternative to the 40-base pair PNA-DNA heteroduplex and 24-base pair DNA-DNA stem. Successful strand displacement creates a double-stranded Bcc I restriction endonuclease cleavage site. The Bcc I-mediated cleavage of the primer extension product (lanes 4 and 8) is therefore consistent with displacement of the PNA strand. The red dot represents a Cy3 backbone spacer that blocks subsequentual DNA polymerization. The green dot represents a fluorescein group. The denaturing PAGE gel shown is visualized by imaging fluorescein fluorescence. Reactions shown in lanes labeled “translation–” lacked NaCNBH 3 ; those in lanes labeled “displacement–” lacked DNA polymerase; those in lanes labeled “restriction digestion–” lacked Bcc I.

    Journal: Nature chemical biology

    Article Title: An In Vitro Translation, Selection, and Amplification System for Peptide Nucleic Acids

    doi: 10.1038/nchembio.280

    Figure Lengend Snippet: Translation of a DNA template into PNA and displacement of the resulting PNA strand Following PNA polymerization (lane 5) under conditions described in Figure 2 , the PNA strand is displaced by primer extension of the 3′ hairpin using Herculase DNA polymerase II and dNTPs (lane 6). DNA polymerization to the end of the template strand (through the 5′ stem-loop) generates an 84-base pair DNA duplex that can form as a mutually exclusive alternative to the 40-base pair PNA-DNA heteroduplex and 24-base pair DNA-DNA stem. Successful strand displacement creates a double-stranded Bcc I restriction endonuclease cleavage site. The Bcc I-mediated cleavage of the primer extension product (lanes 4 and 8) is therefore consistent with displacement of the PNA strand. The red dot represents a Cy3 backbone spacer that blocks subsequentual DNA polymerization. The green dot represents a fluorescein group. The denaturing PAGE gel shown is visualized by imaging fluorescein fluorescence. Reactions shown in lanes labeled “translation–” lacked NaCNBH 3 ; those in lanes labeled “displacement–” lacked DNA polymerase; those in lanes labeled “restriction digestion–” lacked Bcc I.

    Article Snippet: Herculase II DNA polymerase (Stratagene), a fusion protein of Pfu Ultra and a DNA-binding domain that is designed to facilitate DNA polymerization on GC-rich templates, was able to displace the PNA strand efficiently ( ).

    Techniques: Polyacrylamide Gel Electrophoresis, Imaging, Fluorescence, Labeling

    Quantitative assessment of viral DNA in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 pfu of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Comparative assessment of virulence of recombinant vaccinia viruses expressing IL-2 and IL-15 in immunodeficient mice

    doi: 10.1073/pnas.081080298

    Figure Lengend Snippet: Quantitative assessment of viral DNA in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 pfu of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.

    Article Snippet: Five micrograms of cellular DNA was used in PCR amplification of the Escherichia coli β-galactosidase DNA sequences with Pfu Turbo DNA polymerase (Stratagene).

    Techniques: Infection, Mouse Assay, Recombinant, Isolation, Polymerase Chain Reaction, Amplification

    Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a PCR at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate DNA bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.

    Journal: Journal of Bacteriology

    Article Title: Identification of a Differentially Expressed Oligopeptide Binding Protein (OppA2) in Streptococcus uberis by Representational Difference Analysis of cDNA

    doi: 10.1128/JB.185.17.5210-5219.2003

    Figure Lengend Snippet: Cloning the full-length oppA2 gene. (a) Schematic of how 5′ and 3′ regions of the oppA2 gene were cloned. Gene-specific primers SP1 and SP2 (HAP F and HAP R) were used in concert with primers directed against pneumococcal sequences RP1 or RP2 and were used in a PCR at low annealing temperature to encourage mispriming of the pneumococcal primers. (b) Cloning the 3′ oppA2 region. Primer RP2 misprimed close to the oppA2 gene and generated a product that was detected by Southern blotting by using pDub3 insert as a probe (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products). (c) Cloning the 5′ sequence of oppA2 (lane M, GeneRuler 1-kb ladder; center lane, ethidium bromide-stained agarose gel of PCR products; and rightmost lane, Southern blot of PCR products probed with pDub3). Arrowheads indicate DNA bands taken for cloning. Molecular mass of GeneRuler 1-kb ladder (MBI) shown in kilobases.

    Article Snippet: An additional primer, oppA6, was then designed upstream of the ATG and was used in a PCR with primer oppA4 and a proofreading DNA polymerase, Pfu Turbo (Stratagene), to amplify the full-length gene product, oppA2 .

    Techniques: Clone Assay, Polymerase Chain Reaction, Generated, Southern Blot, Staining, Agarose Gel Electrophoresis, Sequencing

    Non-overlapping regulatory modules within angptl4 intron 3 confer liver, islet, and enterocyte-specific reporter expression. (A) Depiction of the 6 dpf zebrafish showing liver (li, green), intestine (in, blue), swim bladder (sb, grey), and muscle (mu, grey), with the fish oriented anterior (a) to the left and posterior (p) to the right. The opposite orientation reveals the exocrine pancreas (pa, yellow) and islet (is, orange). (B) Scaled schematic of the zebrafish angptl4 locus and non-coding DNA assayed for regulatory potential. Modules are color coded according to the tissues in which they confer expression. Ratios of islet or intestine positive fish versus total fish expressing gfp are shown in parentheses next to truncation labels. (C–N) Representative images of GFP reporter expression in mosaic (column 1) and F 1 stable (column 2) animals driven by each non-coding DNA region (rows). Scale bars = 100 µm; li = liver, is = islet, in = intestine, sb = swim bladder. Colored arrowheads indicate tissue with specific reporter expression. (C–D) Full-length intron 3 (in3; 2,136 bp) is sufficient to promote expression of the reporter in the liver, islet (D, inset, scale bar = 50 µm), and intestine. (E–F) Truncation in3.1 (1,219 bp) confers expression in the liver. (G–H) Truncation in3.2 (701 bp) confers expression in both the intestine and islet (H, inset). Inset scale bar = 50 µm. (I–J) Truncation in3.3 (387 bp) confers islet expression. A transverse section (inset, J) reveals islet expression (nuclei stained with DAPI). Inset scale bar = 50 µm. (K–L) Truncation in3.4 (316 bp) confers intestinal expression. Insets in panels K and L contain transverse sections showing expression localized to the intestinal epithelium (nuclei stained with DAPI). Inset scale bar = 25 µm. The dotted lines in panels D, G, H, and I outline the pancreas. The white arrows in panels H, K, and L mark the boundary between the anterior intestine (segment 1) and mid-intestine (segment 2). (M–N) Cells expressing GFP driven by the in3.4 regulatory module colocalize with a marker (4E8, red, white arrow) of the brush border of absorptive enterocytes, but fail to co-localize with marker for secretory cells (2F11, red, asterisk). Nuclei stained with DAPI. Scale bars = 5 µm. (O) Intercross of Tg(in3.2-Mmu.Fos:tdTomato) with β-cell specific reporter line ( Tg(ins:CFP-NTR) s892 ) show colocalization of tdTomato and CFP in the islet. Scale bars = 10 µm. (P) Quantitative PCR shows that the in3.4 module and the angptl4 promoter (TATA box), but not the in3.3 module, are hypersensitive to DNase I cleavage in intestinal epithelial cells isolated from adult zebrafish. Asterisks denote P-value

    Journal: PLoS Genetics

    Article Title: Intronic Cis-Regulatory Modules Mediate Tissue-Specific and Microbial Control of angptl4/fiaf Transcription

    doi: 10.1371/journal.pgen.1002585

    Figure Lengend Snippet: Non-overlapping regulatory modules within angptl4 intron 3 confer liver, islet, and enterocyte-specific reporter expression. (A) Depiction of the 6 dpf zebrafish showing liver (li, green), intestine (in, blue), swim bladder (sb, grey), and muscle (mu, grey), with the fish oriented anterior (a) to the left and posterior (p) to the right. The opposite orientation reveals the exocrine pancreas (pa, yellow) and islet (is, orange). (B) Scaled schematic of the zebrafish angptl4 locus and non-coding DNA assayed for regulatory potential. Modules are color coded according to the tissues in which they confer expression. Ratios of islet or intestine positive fish versus total fish expressing gfp are shown in parentheses next to truncation labels. (C–N) Representative images of GFP reporter expression in mosaic (column 1) and F 1 stable (column 2) animals driven by each non-coding DNA region (rows). Scale bars = 100 µm; li = liver, is = islet, in = intestine, sb = swim bladder. Colored arrowheads indicate tissue with specific reporter expression. (C–D) Full-length intron 3 (in3; 2,136 bp) is sufficient to promote expression of the reporter in the liver, islet (D, inset, scale bar = 50 µm), and intestine. (E–F) Truncation in3.1 (1,219 bp) confers expression in the liver. (G–H) Truncation in3.2 (701 bp) confers expression in both the intestine and islet (H, inset). Inset scale bar = 50 µm. (I–J) Truncation in3.3 (387 bp) confers islet expression. A transverse section (inset, J) reveals islet expression (nuclei stained with DAPI). Inset scale bar = 50 µm. (K–L) Truncation in3.4 (316 bp) confers intestinal expression. Insets in panels K and L contain transverse sections showing expression localized to the intestinal epithelium (nuclei stained with DAPI). Inset scale bar = 25 µm. The dotted lines in panels D, G, H, and I outline the pancreas. The white arrows in panels H, K, and L mark the boundary between the anterior intestine (segment 1) and mid-intestine (segment 2). (M–N) Cells expressing GFP driven by the in3.4 regulatory module colocalize with a marker (4E8, red, white arrow) of the brush border of absorptive enterocytes, but fail to co-localize with marker for secretory cells (2F11, red, asterisk). Nuclei stained with DAPI. Scale bars = 5 µm. (O) Intercross of Tg(in3.2-Mmu.Fos:tdTomato) with β-cell specific reporter line ( Tg(ins:CFP-NTR) s892 ) show colocalization of tdTomato and CFP in the islet. Scale bars = 10 µm. (P) Quantitative PCR shows that the in3.4 module and the angptl4 promoter (TATA box), but not the in3.3 module, are hypersensitive to DNase I cleavage in intestinal epithelial cells isolated from adult zebrafish. Asterisks denote P-value

    Article Snippet: Reporter construct cloning All PCR reactions used for cloning were performed with high-fidelity DNA polymerase (PfuTurbo, Stratagene; Phusion, Invitrogen; Platinum Taq, Invitrogen) and TOP10 chemically competent E. coli (Invitrogen).

    Techniques: Expressing, Fluorescence In Situ Hybridization, Staining, Marker, Real-time Polymerase Chain Reaction, Isolation

    Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with HCMV, incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV DNA per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Establishment of a Cell-Based Assay for Screening of Compounds Inhibiting Very Early Events in the Cytomegalovirus Replication Cycle and Characterization of a Compound Identified Using the Assay

    doi: 10.1128/AAC.00134-08

    Figure Lengend Snippet: Effect of DPPC on the entry process. hTERT-BJ1 cells that were pretreated with DMSO, 20 μM DPPC, or 1 mg/ml heparan sulfate (HS) were infected with HCMV, incubated at 4°C for 2 h, rinsed twice with PBS to remove unbound virions, and then cultured at 37°C for 2 h. The drugs were present throughout the processes. The cells were rinsed with PBS and then treated with 5 mg/ml protease K and 1 mg/ml DNase I in PBS containing 5 mM MgCl 2 at 37°C for 30 min. The cells were harvested by centrifugation. The amount of HCMV DNA per cell was measured by qPCR assays for HCMV and cellular genes. Means and SDs of the DNA amounts obtained in triplicate experiments are shown.

    Article Snippet: DNA fragments containing HCMV promoters were amplified from the nucleocapsid DNA by PCR with native PfuI DNA polymerase (Stratagene) and cloned into a reporter plasmid, pGL3-Basic (Promega), that encodes firefly luciferase.

    Techniques: Infection, Incubation, Cell Culture, Centrifugation, Real-time Polymerase Chain Reaction

    Establishment and characterization of a CMV reporter cell line. (A) Selection of an HCMV promoter for establishment of an HCMV reporter cell line. DNA fragments containing the HCMV promoters for the indicated open reading frames were amplified by PCR and cloned into the luciferase reporter plasmid pGL3-Basic (Promega). U373MG glioma cells were transfected with the plasmids and then infected with HCMV at 24 h after transfection. Luciferase activities were measured at 24 h p.i. Means and SDs in RLU from triplicate experiments are shown. (B) U4C-48 cells (2.8 × 10 4 cells/well) in 96-well plates were infected with serial dilutions of HCMV (Towne) and cultured for 45 h prior to measurement of luciferase activity. Means and SDs in RLU from triplicate experiments are shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Establishment of a Cell-Based Assay for Screening of Compounds Inhibiting Very Early Events in the Cytomegalovirus Replication Cycle and Characterization of a Compound Identified Using the Assay

    doi: 10.1128/AAC.00134-08

    Figure Lengend Snippet: Establishment and characterization of a CMV reporter cell line. (A) Selection of an HCMV promoter for establishment of an HCMV reporter cell line. DNA fragments containing the HCMV promoters for the indicated open reading frames were amplified by PCR and cloned into the luciferase reporter plasmid pGL3-Basic (Promega). U373MG glioma cells were transfected with the plasmids and then infected with HCMV at 24 h after transfection. Luciferase activities were measured at 24 h p.i. Means and SDs in RLU from triplicate experiments are shown. (B) U4C-48 cells (2.8 × 10 4 cells/well) in 96-well plates were infected with serial dilutions of HCMV (Towne) and cultured for 45 h prior to measurement of luciferase activity. Means and SDs in RLU from triplicate experiments are shown.

    Article Snippet: DNA fragments containing HCMV promoters were amplified from the nucleocapsid DNA by PCR with native PfuI DNA polymerase (Stratagene) and cloned into a reporter plasmid, pGL3-Basic (Promega), that encodes firefly luciferase.

    Techniques: Selection, Amplification, Polymerase Chain Reaction, Clone Assay, Luciferase, Plasmid Preparation, Transfection, Infection, Cell Culture, Activity Assay

    Effect of DPPC on HCMV DNA replication. hTERT-BJ1 cells were infected with HCMV at an MOI of 0.05 (A) or at an MOI of 3.0 (B) and cultured for the indicated durations in the presence of DMSO or 20 μM or 5 μM of DPPC or GCV. Copy numbers for HCMV UL83 and human albumin genes were obtained by qPCR assays. HCMV genome copy numbers divided by albumin gene copy numbers were expressed as HCMV copy numbers per cell. Means and SDs of the copy numbers obtained in triplicate experiments are shown.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Establishment of a Cell-Based Assay for Screening of Compounds Inhibiting Very Early Events in the Cytomegalovirus Replication Cycle and Characterization of a Compound Identified Using the Assay

    doi: 10.1128/AAC.00134-08

    Figure Lengend Snippet: Effect of DPPC on HCMV DNA replication. hTERT-BJ1 cells were infected with HCMV at an MOI of 0.05 (A) or at an MOI of 3.0 (B) and cultured for the indicated durations in the presence of DMSO or 20 μM or 5 μM of DPPC or GCV. Copy numbers for HCMV UL83 and human albumin genes were obtained by qPCR assays. HCMV genome copy numbers divided by albumin gene copy numbers were expressed as HCMV copy numbers per cell. Means and SDs of the copy numbers obtained in triplicate experiments are shown.

    Article Snippet: DNA fragments containing HCMV promoters were amplified from the nucleocapsid DNA by PCR with native PfuI DNA polymerase (Stratagene) and cloned into a reporter plasmid, pGL3-Basic (Promega), that encodes firefly luciferase.

    Techniques: Infection, Cell Culture, Real-time Polymerase Chain Reaction