dna polymerase Search Results


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  • 99
    New England Biolabs phusion high fidelity dna polymerase
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 24256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher taq polymerase
    Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33062 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq polymerase
    Platinum Taq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa taq polymerase
    Taq Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10064 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega taq polymerase
    Taq Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 18606 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs taq polymerase
    Taq Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8780 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher phusion high fidelity dna polymerase
    Phusion High Fidelity Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 18947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa ex taq dna polymerase
    Ex Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 9323 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen taq dna polymerase
    Taq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 8578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amplitaq gold dna polymerase
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene pfu dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Pfu Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 11421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs q5 high fidelity dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Q5 High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9046 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa la taq dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    La Taq Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5238 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    T4 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9070 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa primestar hs dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Primestar Hs Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 7828 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pfu polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Pfu Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen hotstartaq dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Hotstartaq Dna Polymerase, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum taq polymerase high fidelity
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Platinum Taq Polymerase High Fidelity, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher platinum pfx dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Platinum Pfx Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7813 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega gotaq flexi dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Gotaq Flexi Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 7095 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna polymerase
    <t>Pfu</t> P45 enhances PCRs using archaeal <t>DNA</t> polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.
    Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4972 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Stratagene pfu turbo dna polymerase
    Quantitative assessment of viral <t>DNA</t> in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 <t>pfu</t> of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.
    Pfu Turbo Dna Polymerase, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 6343 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Pfu P45 enhances PCRs using archaeal DNA polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

    doi: 10.1073/pnas.012372799

    Figure Lengend Snippet: Pfu P45 enhances PCRs using archaeal DNA polymerases. Native P45 (0, 1, 5, or 10 ng) was added to amplifications of 0.9-kb and 1.9-kb genomic targets. PCRs were carried out with 2.5 units of Pfu DNA polymerase or 1 unit of Vent R , Deep Vent R , or Taq DNA polymerase in each enzyme's optimal PCR buffer.

    Article Snippet: Improvements in the performance of Pfu DNA polymerase have been achieved by increasing PCR extension times (2 min/kb of target; Stratagene Pfu DNA polymerase manual), incorporating additives such as DMSO ( , ), and mixing Pfu with Taq DNA polymerase ( ).

    Techniques: Polymerase Chain Reaction

    Human dUTPase exhibits PCR-enhancing activity. A 5.2-kb target was amplified with 5 units of Pfu DNA polymerase. At each annealing step (58°C), 0.5 μl of the following was added: buffer (lane 1) or human dUTPase preparation, undiluted (lanes 2 and 3), or diluted 10 −1 (lanes 4 and 5).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

    doi: 10.1073/pnas.012372799

    Figure Lengend Snippet: Human dUTPase exhibits PCR-enhancing activity. A 5.2-kb target was amplified with 5 units of Pfu DNA polymerase. At each annealing step (58°C), 0.5 μl of the following was added: buffer (lane 1) or human dUTPase preparation, undiluted (lanes 2 and 3), or diluted 10 −1 (lanes 4 and 5).

    Article Snippet: Improvements in the performance of Pfu DNA polymerase have been achieved by increasing PCR extension times (2 min/kb of target; Stratagene Pfu DNA polymerase manual), incorporating additives such as DMSO ( , ), and mixing Pfu with Taq DNA polymerase ( ).

    Techniques: Polymerase Chain Reaction, Activity Assay, Amplification

    Pfu P45 improves amplification of long targets by Pfu DNA polymerase. PCRs were performed with Taq or Pfu DNA polymerase, in the absence or presence of P45, in each enzyme's recommended buffer. The following PCR products were amplified: lanes 1–4, 7–10, and 13–16, 0.9- to 5.2-kb fragments of the human α-1-antitrypsin gene; lanes 5, 11, and 17, 8-kb fragment from mouse genomic DNA; and lanes 6, 12, and 18, 14-kb fragment from λ phage DNA. PCR amplifications were conducted by using 2.5 units (lanes 4, 10, and 16) or 5 units of each enzyme and an extension time of 1 min/kb of target.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

    doi: 10.1073/pnas.012372799

    Figure Lengend Snippet: Pfu P45 improves amplification of long targets by Pfu DNA polymerase. PCRs were performed with Taq or Pfu DNA polymerase, in the absence or presence of P45, in each enzyme's recommended buffer. The following PCR products were amplified: lanes 1–4, 7–10, and 13–16, 0.9- to 5.2-kb fragments of the human α-1-antitrypsin gene; lanes 5, 11, and 17, 8-kb fragment from mouse genomic DNA; and lanes 6, 12, and 18, 14-kb fragment from λ phage DNA. PCR amplifications were conducted by using 2.5 units (lanes 4, 10, and 16) or 5 units of each enzyme and an extension time of 1 min/kb of target.

    Article Snippet: Improvements in the performance of Pfu DNA polymerase have been achieved by increasing PCR extension times (2 min/kb of target; Stratagene Pfu DNA polymerase manual), incorporating additives such as DMSO ( , ), and mixing Pfu with Taq DNA polymerase ( ).

    Techniques: Amplification, Polymerase Chain Reaction

    PCR-enhancing activity from Pfu . Fractions eluted from heparin-Sepharose ( A ) or Sephacryl S-200 ( B ) columns were added to PCRs using Pfu DNA polymerase. ( A ) A 6.6-kb (lanes 1–8) or 5.2-kb (lanes 9–10) genomic target was amplified in the presence (+) of the following amount of eluate: lanes 1, 3, and 9, none (−); lanes 2, 4, 5, and 10, 1 μl of undiluted; lane 6, 1 μl of diluted 10 −1 ; lane 7, 1 μl of diluted 10 −3 ; and lane 8, 1 μl of diluted 10 −5 . As negative controls, either Pfu DNA polymerase ( A , lanes 1 and 2) or genomic DNA ( A , lanes 3 and 4) was omitted. The 6.6-kb PCR system produces a second nonspecific band (lower band in lanes 5 and 6) irrespective of Pfu P45 addition. ( B ) The following amount (ng) of S-200-purified protein was added (+) to amplifications of an 8-kb genomic target: lane 1, none (−); lane 2, 0.1; lane 3, 0.2; lane 4, 0.5; lane 5, 1; lane 6, 5; lane 7, 25; lane 8, 100; or lane 9, 200.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Archaeal dUTPase enhances PCR amplifications with archaeal DNA polymerases by preventing dUTP incorporation

    doi: 10.1073/pnas.012372799

    Figure Lengend Snippet: PCR-enhancing activity from Pfu . Fractions eluted from heparin-Sepharose ( A ) or Sephacryl S-200 ( B ) columns were added to PCRs using Pfu DNA polymerase. ( A ) A 6.6-kb (lanes 1–8) or 5.2-kb (lanes 9–10) genomic target was amplified in the presence (+) of the following amount of eluate: lanes 1, 3, and 9, none (−); lanes 2, 4, 5, and 10, 1 μl of undiluted; lane 6, 1 μl of diluted 10 −1 ; lane 7, 1 μl of diluted 10 −3 ; and lane 8, 1 μl of diluted 10 −5 . As negative controls, either Pfu DNA polymerase ( A , lanes 1 and 2) or genomic DNA ( A , lanes 3 and 4) was omitted. The 6.6-kb PCR system produces a second nonspecific band (lower band in lanes 5 and 6) irrespective of Pfu P45 addition. ( B ) The following amount (ng) of S-200-purified protein was added (+) to amplifications of an 8-kb genomic target: lane 1, none (−); lane 2, 0.1; lane 3, 0.2; lane 4, 0.5; lane 5, 1; lane 6, 5; lane 7, 25; lane 8, 100; or lane 9, 200.

    Article Snippet: Improvements in the performance of Pfu DNA polymerase have been achieved by increasing PCR extension times (2 min/kb of target; Stratagene Pfu DNA polymerase manual), incorporating additives such as DMSO ( , ), and mixing Pfu with Taq DNA polymerase ( ).

    Techniques: Polymerase Chain Reaction, Activity Assay, Amplification, Purification

    Quantitative assessment of viral DNA in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 pfu of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Comparative assessment of virulence of recombinant vaccinia viruses expressing IL-2 and IL-15 in immunodeficient mice

    doi: 10.1073/pnas.081080298

    Figure Lengend Snippet: Quantitative assessment of viral DNA in tissues of infected mice. Nude mice were inoculated i.v. with 10 6 pfu of the respective recombinant vaccinia viruses, and tissues were harvested 72 h after infection. Genomic DNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4). Each sample represents tissues pooled from three animals, and the recovery of DNA was first standardized to the cytochrome- c oxidase II gene content in each sample before PCR amplification of the β-galactosidase gene.

    Article Snippet: Five micrograms of cellular DNA was used in PCR amplification of the Escherichia coli β-galactosidase DNA sequences with Pfu Turbo DNA polymerase (Stratagene).

    Techniques: Infection, Mouse Assay, Recombinant, Isolation, Polymerase Chain Reaction, Amplification