dna lo-bind tube Search Results


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  • 99
    Millipore dna lobind tube
    Dna Lobind Tube, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna lobind tube/product/Millipore
    Average 99 stars, based on 5 article reviews
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    94
    Eppendorf AG dna lo bind tube
    Dna Lo Bind Tube, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna lo bind tube/product/Eppendorf AG
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    94
    Eppendorf AG lo bind tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Lo Bind Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 94/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lo bind tubes/product/Eppendorf AG
    Average 94 stars, based on 147 article reviews
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    86
    Fisher Scientific dna lo bind tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Dna Lo Bind Tubes, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna lo bind tubes/product/Fisher Scientific
    Average 86 stars, based on 4 article reviews
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    99
    Eppendorf AG dna lo binding tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Dna Lo Binding Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna lo binding tubes/product/Eppendorf AG
    Average 99 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    dna lo binding tubes - by Bioz Stars, 2020-04
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    99
    Millipore dna lobind tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Dna Lobind Tubes, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna lobind tubes/product/Millipore
    Average 99 stars, based on 5 article reviews
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    dna lobind tubes - by Bioz Stars, 2020-04
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    90
    Eppendorf AG dna lobind nuclease free tube
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Dna Lobind Nuclease Free Tube, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 14 article reviews
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    99
    Eppendorf AG lobind eppendorf tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Lobind Eppendorf Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lobind eppendorf tubes/product/Eppendorf AG
    Average 99 stars, based on 217 article reviews
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    93
    Eppendorf AG sample to surface binding tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Sample To Surface Binding Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Eppendorf AG low binding microcentrifugation tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Low Binding Microcentrifugation Tubes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rna isolation
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Rna Isolation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 52893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher i5 i7 index primers
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    I5 I7 Index Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Corning Life Sciences pcr tubes
    Storage condition of purified ChIP <t>DNA</t> is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; <t>LoBind,</t> Eppendorf DNA LoBind Snap Cap <t>PCR</t> Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube
    Pcr Tubes, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: Storage condition of purified ChIP DNA is important. Purified ChIP DNA was adjusted to a concentration of 1 ng/μL ( a ) or 0.1 ng/μL ( b ), aliquoted into 4 different types of microcentrifuge tubes in 15 μL volume, and stored at −20 °C. DNA was quantified using Qubit dsDNA High Sensitivity assay at the indicated time points and expressed as a percentage of the amount measured at day 0. Three independent DNA samples were used in the experiment and DNA concentration from five tubes were measured at each time point. MaxyClear, Axygen® 1.7 mL MaxyClear Snaplock Microcentrifuge Tube; LoBind, Eppendorf DNA LoBind Snap Cap PCR Tube; Siliconized, Fisherbrand™ Siliconized Low-Retention Microcentrifuge Tube; Premium, Fisherbrand™ Premium Microcentrifuge Tube

    Article Snippet: Eppendorf DNA LoBind Snap Cap PCR Tubes (LoBind) performed similarly well at −20 °C, preserving 91.7%, 84.6%, and 83.7% of DNA over the same time frame.

    Techniques: Purification, Chromatin Immunoprecipitation, Concentration Assay, Sensitive Assay, Polymerase Chain Reaction

    DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Journal: BMC Genomics

    Article Title: Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

    doi: 10.1186/s12864-017-4371-5

    Figure Lengend Snippet: DNA purification reagents vary in their ability to recover low amounts of DNA from de-crosslinked chromatin. a Recovered DNA amount by different DNA purification reagents from de-crosslinked chromatin. De-crosslinked chromatin estimated to include 1 ng range DNA in ChIP elution buffer was purified following the manufacturer’s instructions. The data were generated from triplicate DNA samples derived from three independent preparations. Zy, ChIP DNA Clean Concentrator™ (Zymo Research); Pr, Wizard® SV Gel and PCR Clean-Up System (Promega); Th, GeneJET PCR Purification Kit (Thermo Fisher Scientific); In, PureLink® PCR Purification Kit (Invitrogen); Ne, Monarch® PCR DNA Cleanup Kit (New England Biolabs); Am, Chromatin IP DNA Purification Kit (Active Motif); Qp, QIAquick PCR Purification Kit (Qiagen); Qm, MinElute PCR Purification Kit (Qiagen); Ba, Agencourt AMPure XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); Br, RNAClean™ XP kit (Beckman, chromatin to beads ratio from 1:1.25 to 1:2); PC, phenol/chloroform extraction. b Interference of PCR amplification by purified eluent of purification reagents. 9 μL eluent was mixed with 1 μL 166 bp of Drosophila probe DNA (0.0001 ng), and the resulting mixture was used as the template in 20 μl of real-time PCR reaction. The Ct value for Drosophila probe DNA from TE buffer was set as 100%. The experiment was repeated 3 times using de-crosslinked chromatin estimated to include 1 ng of DNA. c Size profiles of DNA purified by different reagents. The DNAs purified from de-crosslinked chromatin estimated to include 50 ng range DNA was analyzed by AATI Fragment Analyzer. DNA size (bp) is shown

    Article Snippet: Eppendorf DNA LoBind Snap Cap PCR Tubes (LoBind) performed similarly well at −20 °C, preserving 91.7%, 84.6%, and 83.7% of DNA over the same time frame.

    Techniques: DNA Purification, Chromatin Immunoprecipitation, Purification, Generated, Derivative Assay, Polymerase Chain Reaction, Amplification, Real-time Polymerase Chain Reaction

    Binding affinities and elution of the S1 and S1m aptamers ( A ) In vitro binding of recombinant SA protein to S1 and S1m RNA was measured by EMSA. Radiolabeled S1 and S1m RNAs were incubated with increasing concentrations of SA. Free RNA (black arrow) was separated from RNA-protein complexes (white arrow) by native 6% PAGE. Bottom graph: the apparent dissociation constant K d ± SD was calculated from quantification of three independent experiments. ( B ) Schematic representation of DNA templates used for in vitro transcription of 4×S1, 4×S1m, ARE-4×S1 and ARE-4×S1m RNAs. ( C ) Elution by biotin was examined by in vitro SA pulldown experiments. In vitro transcribed RNAs were coupled to SA-Sepharose beads, incubated with NIH3T3 cell extracts, washed and eluted using 10 mM biotin (E B ). Residual RNA bound to beads was subsequently eluted with Trizol (E T ). RNA was resolved by 6% denaturing urea-PAGE and visualized by methylene blue staining. Each lane represents 20% of the starting amount, low range ssRNA ladder (NEB) was loaded for reference. On the right, the percentage of RNA recovered at each step was quantified based on three independent repeat experiments, average values ± SD are shown. ( D ) The same experiment was carried out as in C, except that the first elution was carried out with 0.05 µg/µl RNase A. ( E ) Upon RNA affinity purification using an NIH3T3 cell lysate as in C, proteins were eluted with RNase A followed by subsequent elution with SDS to determine residual protein bound to the beads. Top: 10% of each RNA fraction was resolved by denaturing urea-PAGE and stained with methylene blue. Bottom: 23% of the RNase A and SDS eluates was resolved by PAGE and proteins were monitored by western blot analysis. The ARE-BP HuR serves as positive control, whereas G3BP is a negative control.

    Journal: Nucleic Acids Research

    Article Title: An optimized streptavidin-binding RNA aptamer for purification of ribonucleoprotein complexes identifies novel ARE-binding proteins

    doi: 10.1093/nar/gkt956

    Figure Lengend Snippet: Binding affinities and elution of the S1 and S1m aptamers ( A ) In vitro binding of recombinant SA protein to S1 and S1m RNA was measured by EMSA. Radiolabeled S1 and S1m RNAs were incubated with increasing concentrations of SA. Free RNA (black arrow) was separated from RNA-protein complexes (white arrow) by native 6% PAGE. Bottom graph: the apparent dissociation constant K d ± SD was calculated from quantification of three independent experiments. ( B ) Schematic representation of DNA templates used for in vitro transcription of 4×S1, 4×S1m, ARE-4×S1 and ARE-4×S1m RNAs. ( C ) Elution by biotin was examined by in vitro SA pulldown experiments. In vitro transcribed RNAs were coupled to SA-Sepharose beads, incubated with NIH3T3 cell extracts, washed and eluted using 10 mM biotin (E B ). Residual RNA bound to beads was subsequently eluted with Trizol (E T ). RNA was resolved by 6% denaturing urea-PAGE and visualized by methylene blue staining. Each lane represents 20% of the starting amount, low range ssRNA ladder (NEB) was loaded for reference. On the right, the percentage of RNA recovered at each step was quantified based on three independent repeat experiments, average values ± SD are shown. ( D ) The same experiment was carried out as in C, except that the first elution was carried out with 0.05 µg/µl RNase A. ( E ) Upon RNA affinity purification using an NIH3T3 cell lysate as in C, proteins were eluted with RNase A followed by subsequent elution with SDS to determine residual protein bound to the beads. Top: 10% of each RNA fraction was resolved by denaturing urea-PAGE and stained with methylene blue. Bottom: 23% of the RNase A and SDS eluates was resolved by PAGE and proteins were monitored by western blot analysis. The ARE-BP HuR serves as positive control, whereas G3BP is a negative control.

    Article Snippet: For the following procedures, DNA/RNA LoBind tubes (Eppendorf) were used to reduce the degree of unspecific binding.

    Techniques: Binding Assay, In Vitro, Recombinant, Incubation, Polyacrylamide Gel Electrophoresis, Staining, Affinity Purification, Western Blot, Positive Control, Negative Control