Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: A very large protein with diverse functional motifs is deficient in rjs (runty, jerky, sterile) mice

doi:

Figure Lengend Snippet: Northern blot of total RNA isolated from wild-type and p 6H / p 6H tissues (B, brain; L, liver; and T, testis) and hybridized with BS2c probe (corresponding to nucleotides 12661–14606 of the final rjs cDNA contig, which includes part of RLDc and all of the HECT domain). Markers (not shown) indicate this to be a very large transcript, ≥15 kb. Note the expression of the rjs gene in brain and testis of wild type and the absence of transcript in p 6H / p 6H total RNA. The faint high molecular weight band in p 6H / p 6H brain total RNA probably represents nonspecific hybridization since RT-PCR from p 6H / p 6H mRNA was negative with primers after nucleotide 1993.

Article Snippet: After confirmation by Southern and Northern blots, the insert from the largest of these clones (BS2c) was used to screen two additional randomly primed cDNA libraries prepared from testis [gift of T. Miki, National Institutes of Health ( )] and 16.5-day embryos (from Stratagene).

Techniques: Northern Blot, Isolation, Expressing, Molecular Weight, Hybridization, Reverse Transcription Polymerase Chain Reaction

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: A very large protein with diverse functional motifs is deficient in rjs (runty, jerky, sterile) mice

doi:

Figure Lengend Snippet: Southern blot analysis of genomic DNA from wild type, p 6H / p 6H , p bs / p bs , p cp / p cp , and p 25H / p 25H homozygous mice digested with Hin dIII and hybridized with the BS2c probe (corresponding to nucleotides 12661–14606 of the final rjs cDNA contig that includes part of RLDc and all of the HECT domain). Marker lane contains size markers with position in kb indicated at left. Note deletion of all bands except lowest molecular weight band in p 6H / p 6H DNA.

Article Snippet: After confirmation by Southern and Northern blots, the insert from the largest of these clones (BS2c) was used to screen two additional randomly primed cDNA libraries prepared from testis [gift of T. Miki, National Institutes of Health ( )] and 16.5-day embryos (from Stratagene).

Techniques: Southern Blot, Mouse Assay, Marker, Molecular Weight