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  • 83
    New England Biolabs fast dna ladder
    Fast Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 83/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher 100 bp ladder maker
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    100 Bp Ladder Maker, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna standard
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    Dna Standard, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Roche 100 bp dna ladder
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    100 Bp Dna Ladder, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs kb dna ladder
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    Kb Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bp tridye dna ladder
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    Bp Tridye Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    New England Biolabs quickload dna ladder
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    Quickload Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs quick load purple 100 bp dna ladder
    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were <t>100</t> bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).
    Quick Load Purple 100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were 100 bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).

    Journal: PLoS ONE

    Article Title: No Evidence for AID/MBD4-Coupled DNA Demethylation in Zebrafish Embryos

    doi: 10.1371/journal.pone.0114816

    Figure Lengend Snippet: Absence of genome demethylation following the injection of a methylated DNA fragment into zebrafish eggs. (A) A schematic structure of the 736-bp DNA template used for in vitro methylation by HpaII methylase to generate M-DNA. Numbers below the horizontal line indicate the positions of HpaII/MspI sites subject to methylation. Numbers above the line show the lengths of the fragments generated when completely digested with HpaII or MspI. (B) Verification of in vitro methylation of the 736-bp DNA template. The unmethylated DNA fragment (U) was susceptible to HpaII, a methylation-sensitive restriction enzyme, as well as MspI, the methylation-insensitive isoschizomer of HpaII. Methylation of the fragment (Me) conferred resistance to DNA to the digestion by HpaII. (C) Undigested genomic DNA of control (W), M-DNA-injected (M), and dnmt1 MO-injected (MO) embryos at the indicated time points (hours post fertilization; hpf) were run on an agarose gel. (D, E, F) The same genomic DNAs as those used and shown in (C) were digested with MspI (D), HpaII (E), or HpyCH4IV (F), and run on an agarose gel. The molecular weight markers of DNA loaded on the first lanes of gels, shown as MW, were 100 bp (B) or 1 kb ladders (C, D, E, and F). Note that smearing down of high-molecular DNA, thereby reducing the methylation level, was discernible only in the genomic DNA from dnmt1 MO-injected embryos (white arrows in E and F).

    Article Snippet: DNA-size markers and restriction enzymes were purchased from New England Biolabs, except for the 100-bp ladder maker in (Invitrogen).

    Techniques: Injection, Methylation, In Vitro, Generated, Agarose Gel Electrophoresis, Molecular Weight