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  • 80
    Thermo Fisher bp dna ladder
    Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp <t>DNA</t> ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 <t>µl</t> and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.
    Bp Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bp dna ladder/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bp dna ladder - by Bioz Stars, 2022-09
    80/100 stars
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    80
    Thermo Fisher kb plus dna ladder
    Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp <t>DNA</t> ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 <t>µl</t> and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.
    Kb Plus Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kb plus dna ladder/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    kb plus dna ladder - by Bioz Stars, 2022-09
    80/100 stars
      Buy from Supplier

    80
    Thermo Fisher dna ladder
    Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp <t>DNA</t> ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 <t>µl</t> and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.
    Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ladder/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna ladder - by Bioz Stars, 2022-09
    80/100 stars
      Buy from Supplier

    88
    Thermo Fisher 100 bp dna ladder
    Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp <t>DNA</t> ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 <t>µl</t> and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.
    100 Bp Dna Ladder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp dna ladder/product/Thermo Fisher
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    100 bp dna ladder - by Bioz Stars, 2022-09
    88/100 stars
      Buy from Supplier

    Image Search Results


    Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp DNA ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 µl and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.

    Journal: Current protocols in molecular biology

    Article Title: Chromatin Interaction Analysis using Paired-End Tag Sequencing

    doi: 10.1002/0471142727.mb2115s89

    Figure Lengend Snippet: Gel analysis of ChIA-PETs after PCR amplification ( STEP 32 ) A 25 bp DNA ladder is shown in lane 1 for size reference. Lanes 2 and 3 are the PCR products generated after 20 cycles of PCR amplification from 1 µl and 2 µl of bead-immobilized template respectively. 25 cycles were used to generate the PCR products in lanes 4 and 5 program, from 1 µl and 2 µl of beads respectively. ( A ) This is a successful library, as indicated by the bright, well-defined bands at the expected size of 166 bp. ( B ) PCR amplification has failed to yield sufficient ChIA-PET DNA, as seen from the very weak band present in lane 5. This could indicate that PCR conditions need to be optimized, or that library construction has failed.

    Article Snippet: Annealed half-linkers A and B ( ) DNA fragment X of known size (blunt-ended, 5’-phosphorylated, ∼1 – 3 kb) Adapters A and B ( ) Primers ( ) 10 mM dNTPs Nuclease-free water T4 DNA ligase (30 U/µl) (Fermentas) 5× T4 DNA Ligase Buffer with PEG (Invitrogen) T4 DNA Polynucleotide Kinase (10 U/µl) (NEB) 10× T4 DNA Ligase Buffer (NEB) EB buffer (Qiagen) GlycoBlue (15 mg/ml) (Ambion) Absolute ethanol/ 75% ethanol (v/v) MmeI (2 U/µl) (NEB) S-adenosylmethionine (SAM) (NEB) 10× NEBuffer 4 (NEB) Dynabeads M-280 Streptavidin (Invitrogen) 1× and 2× B & W buffer (see recipe) E. coli DNA polymerase I (10 U/µl) (NEB) 10× NEBuffer 2 (NEB) HotStarTaq Master Mix (Qiagen) 25 bp DNA ladder (Invitrogen) 4–20% TBE PAGE gel (10 wells) (Invitrogen) 10× TBE buffer ( APPENDIX 2 ) DNA loading dye QIAquick PCR Purification Kit (Qiagen) TE buffer, pH 8.0 ( APPENDIX 2 ) 0.2-ml PCR tubes 0.6-ml tubes 1.7-ml microcentrifuge tubes DNA LoBind Tubes, 1.5-ml (Eppendorf) Novex Mini-Cell (Invitrogen) Intelli-Mixer RM-2L (Palico Biotech) Magnetic Particle Collector (Invitrogen)

    Techniques: Polymerase Chain Reaction, Amplification, Generated, ChIA Pet Assay