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  • 99
    Qiagen genomic dna
    Genomic Dna, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna/product/Qiagen
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna - by Bioz Stars, 2021-06
    99/100 stars
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    99
    Thermo Fisher genomic dna
    Genomic Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    genomic dna - by Bioz Stars, 2021-06
    99/100 stars
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    99
    TaKaRa gdna eraser
    Gdna Eraser, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gdna eraser/product/TaKaRa
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gdna eraser - by Bioz Stars, 2021-06
    99/100 stars
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    86
    Promega wizard genomic dna purification kit
    Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from rice seeds inoculated with different dilutions of Burkholderia glumae BGR1, Burkholderia gladioli BSR3 and mixed co-inoculation Suspensions from rice seeds inoculated with different dilutions of the tested strains or their mix were collected using sterile distilled water containing 0.03% Tween 20 and used as templates for the PCR. Lane 1, 100 ng <t>gDNA</t> from B. glumae BGR1; lane 2, 100 ng gDNA from B. gladioli BSR3; lane 3, cell suspension of overnight culture from B. glumae ; lane, 4–7, 10 − 1 to 10 − 4 dilutions; lane 8, cell suspension of overnight culture from B. gladioli ; lane 9–12, 10 − 1 to 10 − 4 dilutions; lane 13, 1:1 cell suspension mix from overnight culture from B. glumae and B. gladioli ; lane 14–17, 10 − 1 to 10 − 4 dilutions. M, 1 kb <t>DNA</t> ladder; lane 18, un-inoculated surface sterilized rice seeds as a negative control.
    Wizard Genomic Dna Purification Kit, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wizard genomic dna purification kit/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wizard genomic dna purification kit - by Bioz Stars, 2021-06
    86/100 stars
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    N/A
    This is human genomic DNA isolated from whole blood of multiple normal subjects It is suitable for Southern blot hybridizations genomic analysis including PCR and genomic library construction This is
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    N/A
    For rapid and reliable isolation of high quality total cellular DNA from a wide variety of Fungal species and tissues
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    N/A
    PCR DNA hybridization enzyme manipulation cloning Southern blot and array based experiments 50 assays
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    N/A
    For purifying DNA including genomic mitochondrial and viral DNA from buccal cells
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    Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from rice seeds inoculated with different dilutions of Burkholderia glumae BGR1, Burkholderia gladioli BSR3 and mixed co-inoculation Suspensions from rice seeds inoculated with different dilutions of the tested strains or their mix were collected using sterile distilled water containing 0.03% Tween 20 and used as templates for the PCR. Lane 1, 100 ng gDNA from B. glumae BGR1; lane 2, 100 ng gDNA from B. gladioli BSR3; lane 3, cell suspension of overnight culture from B. glumae ; lane, 4–7, 10 − 1 to 10 − 4 dilutions; lane 8, cell suspension of overnight culture from B. gladioli ; lane 9–12, 10 − 1 to 10 − 4 dilutions; lane 13, 1:1 cell suspension mix from overnight culture from B. glumae and B. gladioli ; lane 14–17, 10 − 1 to 10 − 4 dilutions. M, 1 kb DNA ladder; lane 18, un-inoculated surface sterilized rice seeds as a negative control.

    Journal: The Plant Pathology Journal

    Article Title: Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

    doi: 10.5423/PPJ.OA.07.2018.0136

    Figure Lengend Snippet: Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from rice seeds inoculated with different dilutions of Burkholderia glumae BGR1, Burkholderia gladioli BSR3 and mixed co-inoculation Suspensions from rice seeds inoculated with different dilutions of the tested strains or their mix were collected using sterile distilled water containing 0.03% Tween 20 and used as templates for the PCR. Lane 1, 100 ng gDNA from B. glumae BGR1; lane 2, 100 ng gDNA from B. gladioli BSR3; lane 3, cell suspension of overnight culture from B. glumae ; lane, 4–7, 10 − 1 to 10 − 4 dilutions; lane 8, cell suspension of overnight culture from B. gladioli ; lane 9–12, 10 − 1 to 10 − 4 dilutions; lane 13, 1:1 cell suspension mix from overnight culture from B. glumae and B. gladioli ; lane 14–17, 10 − 1 to 10 − 4 dilutions. M, 1 kb DNA ladder; lane 18, un-inoculated surface sterilized rice seeds as a negative control.

    Article Snippet: The gDNA was extracted from cultures of the tested strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), following the manufacturer’s instructions.

    Techniques: Sensitive Assay, Amplification, Polymerase Chain Reaction, Negative Control

    Specificity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR products (A) Products from bacterial genomic DNA. (B) Products from cell suspensions. Lane 1, Burkholderia glumae BGR1; lane 2, B. gladioli BSR3; lane 3, B. cepacia KACC 10189; lane 4, B. cepacia KACC 10190; lane 5, B. cepacia KACC 10337; lane 6, B. cepacia KACC 12679; lane 7, B. cepacia KACC 15010; lane 8, B. kururiensis KACC 12038; lane 9, Burkholderia sp. KJ006; lane 10, B. megalochromosomata KACC 17925; lane 11, B. phymatum KACC 12032; lane 12, B. phytofirmans KACC 12042; lane 13, B. pyrrocinia KACC 17914; lane 14, B. stabilis KACC 12028. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e BGR1 and 289 bp for B. gladioli BSR3. No bands were observed with the other tested Burkholderia species. M denotes the 1 kb DNA ladder.

    Journal: The Plant Pathology Journal

    Article Title: Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

    doi: 10.5423/PPJ.OA.07.2018.0136

    Figure Lengend Snippet: Specificity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR products (A) Products from bacterial genomic DNA. (B) Products from cell suspensions. Lane 1, Burkholderia glumae BGR1; lane 2, B. gladioli BSR3; lane 3, B. cepacia KACC 10189; lane 4, B. cepacia KACC 10190; lane 5, B. cepacia KACC 10337; lane 6, B. cepacia KACC 12679; lane 7, B. cepacia KACC 15010; lane 8, B. kururiensis KACC 12038; lane 9, Burkholderia sp. KJ006; lane 10, B. megalochromosomata KACC 17925; lane 11, B. phymatum KACC 12032; lane 12, B. phytofirmans KACC 12042; lane 13, B. pyrrocinia KACC 17914; lane 14, B. stabilis KACC 12028. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e BGR1 and 289 bp for B. gladioli BSR3. No bands were observed with the other tested Burkholderia species. M denotes the 1 kb DNA ladder.

    Article Snippet: The gDNA was extracted from cultures of the tested strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), following the manufacturer’s instructions.

    Techniques: Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis

    Amplification of the expected PCR products (A) Products from bacterial genomic DNA. (B) Products from suspensions of five strains of Burkholderia glumae (Lane 1–5) amplified using Bglu3-f and Bglu3-r primers and six strains of Burkholderia gladioli (Lane 6–11) amplified using Bgla9-f and Bgla9-r primers. Lane 12 is a negative control. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e and 289 bp for B. gladioli. M denotes the 1 kb DNA ladder.

    Journal: The Plant Pathology Journal

    Article Title: Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

    doi: 10.5423/PPJ.OA.07.2018.0136

    Figure Lengend Snippet: Amplification of the expected PCR products (A) Products from bacterial genomic DNA. (B) Products from suspensions of five strains of Burkholderia glumae (Lane 1–5) amplified using Bglu3-f and Bglu3-r primers and six strains of Burkholderia gladioli (Lane 6–11) amplified using Bgla9-f and Bgla9-r primers. Lane 12 is a negative control. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e and 289 bp for B. gladioli. M denotes the 1 kb DNA ladder.

    Article Snippet: The gDNA was extracted from cultures of the tested strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), following the manufacturer’s instructions.

    Techniques: Amplification, Polymerase Chain Reaction, Negative Control, Agarose Gel Electrophoresis

    Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from genomic DNA (gDNA) from (A) Burkholderia glumae BGR1, (B) B. gladioli BSR3 and (C) mixed sample (1:1) of B. glumae BGR1 and B. gladioli BSR3 in a duplex PCR assay Lane 1, 100 ng gDNA; lane 2, 10 ng gDNA; lane 3, 1 ng gDNA; lane 4, 0.1 ng gDNA; lane 5, 0.01 ng gDNA, lane 6, 1 pg gDNA, lane 7, 0.1 pg; lane 8, no gDNA template. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e and 289 bp for B. gladioli up to the 0.1 ng gDNA template. M denotes the 1 kb DNA ladder.

    Journal: The Plant Pathology Journal

    Article Title: Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

    doi: 10.5423/PPJ.OA.07.2018.0136

    Figure Lengend Snippet: Sensitivity assay for the designed primer pairs (Bglu3 and Bgla9), by amplification of the expected PCR product from genomic DNA (gDNA) from (A) Burkholderia glumae BGR1, (B) B. gladioli BSR3 and (C) mixed sample (1:1) of B. glumae BGR1 and B. gladioli BSR3 in a duplex PCR assay Lane 1, 100 ng gDNA; lane 2, 10 ng gDNA; lane 3, 1 ng gDNA; lane 4, 0.1 ng gDNA; lane 5, 0.01 ng gDNA, lane 6, 1 pg gDNA, lane 7, 0.1 pg; lane 8, no gDNA template. Specific bands were clearly visible on the 2% agarose gel at 174 bp for B. gluma e and 289 bp for B. gladioli up to the 0.1 ng gDNA template. M denotes the 1 kb DNA ladder.

    Article Snippet: The gDNA was extracted from cultures of the tested strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), following the manufacturer’s instructions.

    Techniques: Sensitive Assay, Amplification, Polymerase Chain Reaction, Agarose Gel Electrophoresis