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  • 78
    Thermo Fisher turbo deoxyribonucleic acid dna free kit
    Turbo Deoxyribonucleic Acid Dna Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna free kit
    Phenotype and Analysis of the pumpkin <t>T-DNA</t> Insertion Line. A, Schematic representation of the Arabidopsis PUMPKIN . ATG, start codon; P, Primer; TGA, stop codon. B, Images of the 3-week-old wild type, pumpkin, RNAi, and complemented (Comp) plants grown on soil. White scale bars correspond to 0.5 cm. C, <t>PCR-based</t> genotyping analysis. PCR on genomic DNA with three primers (P1, P2, P3) shown in (A) was conducted in order to distinguish between the wild-type, homozygous ( pumpkin ), and heterozygous (Het) PUMPKIN alleles. P1 and P2 yielded an amplicon of 218 bp corresponding to the expected size of the wild-type allele, while the combination of P2 and P3 amplified the mutated allele (163 bp). D, Analysis of the relative expression of PUMPKIN in the wild type, pumpkin, RNAi, and Comp plants by means of RT-PCR. (D) illustrates that 100%, 10%, and 1% of the wild type correspond to 100 ng, 10 ng, and 1 ng of total RNA used as template for cDNA syntheses. UBIQUITIN ( UBI ) served as control. E, RNA-gel–blot analysis of pumpkin RNA. The PCR probe was amplified with the same primers as in (D). MB, Methylene blue. F, Immunodetection of PUMPKIN in total protein extracts from the wild type, pumpkin, RNAi, and Comp plants using PUMPKIN-specific antibodies. In (F), 100% of the wild type corresponds to 30 µg total proteins. CBB, Coomassie Brilliant Blue. G, Immunodetection of PUMPKIN in stroma extracts (40 µg) from the wild type and pumpkin to prove the specificity of the PUMPKIN antibody. CBB, Coomassie Brilliant Blue; WT, wild type.
    Dna Free Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna dye picogreen
    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Thermo Fisher dna free enzyme
    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing <t>mt-DNA</t> copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of <t>PicoGreen</t> staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p
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    Image Search Results


    Phenotype and Analysis of the pumpkin T-DNA Insertion Line. A, Schematic representation of the Arabidopsis PUMPKIN . ATG, start codon; P, Primer; TGA, stop codon. B, Images of the 3-week-old wild type, pumpkin, RNAi, and complemented (Comp) plants grown on soil. White scale bars correspond to 0.5 cm. C, PCR-based genotyping analysis. PCR on genomic DNA with three primers (P1, P2, P3) shown in (A) was conducted in order to distinguish between the wild-type, homozygous ( pumpkin ), and heterozygous (Het) PUMPKIN alleles. P1 and P2 yielded an amplicon of 218 bp corresponding to the expected size of the wild-type allele, while the combination of P2 and P3 amplified the mutated allele (163 bp). D, Analysis of the relative expression of PUMPKIN in the wild type, pumpkin, RNAi, and Comp plants by means of RT-PCR. (D) illustrates that 100%, 10%, and 1% of the wild type correspond to 100 ng, 10 ng, and 1 ng of total RNA used as template for cDNA syntheses. UBIQUITIN ( UBI ) served as control. E, RNA-gel–blot analysis of pumpkin RNA. The PCR probe was amplified with the same primers as in (D). MB, Methylene blue. F, Immunodetection of PUMPKIN in total protein extracts from the wild type, pumpkin, RNAi, and Comp plants using PUMPKIN-specific antibodies. In (F), 100% of the wild type corresponds to 30 µg total proteins. CBB, Coomassie Brilliant Blue. G, Immunodetection of PUMPKIN in stroma extracts (40 µg) from the wild type and pumpkin to prove the specificity of the PUMPKIN antibody. CBB, Coomassie Brilliant Blue; WT, wild type.

    Journal: Plant Physiology

    Article Title: PUMPKIN, the Sole Plastid UMP Kinase, Associates with Group II Introns and Alters Their Metabolism

    doi: 10.1104/pp.18.00687

    Figure Lengend Snippet: Phenotype and Analysis of the pumpkin T-DNA Insertion Line. A, Schematic representation of the Arabidopsis PUMPKIN . ATG, start codon; P, Primer; TGA, stop codon. B, Images of the 3-week-old wild type, pumpkin, RNAi, and complemented (Comp) plants grown on soil. White scale bars correspond to 0.5 cm. C, PCR-based genotyping analysis. PCR on genomic DNA with three primers (P1, P2, P3) shown in (A) was conducted in order to distinguish between the wild-type, homozygous ( pumpkin ), and heterozygous (Het) PUMPKIN alleles. P1 and P2 yielded an amplicon of 218 bp corresponding to the expected size of the wild-type allele, while the combination of P2 and P3 amplified the mutated allele (163 bp). D, Analysis of the relative expression of PUMPKIN in the wild type, pumpkin, RNAi, and Comp plants by means of RT-PCR. (D) illustrates that 100%, 10%, and 1% of the wild type correspond to 100 ng, 10 ng, and 1 ng of total RNA used as template for cDNA syntheses. UBIQUITIN ( UBI ) served as control. E, RNA-gel–blot analysis of pumpkin RNA. The PCR probe was amplified with the same primers as in (D). MB, Methylene blue. F, Immunodetection of PUMPKIN in total protein extracts from the wild type, pumpkin, RNAi, and Comp plants using PUMPKIN-specific antibodies. In (F), 100% of the wild type corresponds to 30 µg total proteins. CBB, Coomassie Brilliant Blue. G, Immunodetection of PUMPKIN in stroma extracts (40 µg) from the wild type and pumpkin to prove the specificity of the PUMPKIN antibody. CBB, Coomassie Brilliant Blue; WT, wild type.

    Article Snippet: For RT-PCR, DNA contaminations were removed by the DNA-free DNA Removal Kit (Thermo Fisher Scientific, Waltham, MA).

    Techniques: Polymerase Chain Reaction, Amplification, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunodetection

    Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing mt-DNA copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of PicoGreen staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p

    Journal: Cell Death & Disease

    Article Title: Epigenetic modifiers promote mitochondrial biogenesis and oxidative metabolism leading to enhanced differentiation of neuroprogenitor cells

    doi: 10.1038/s41419-018-0396-1

    Figure Lengend Snippet: Sodium butyrate stimulates mitochondrial biogenesis in PC12-ND6 cells by increasing mt-DNA copy number and expression levels of key regulators of mt-DNA replication. a Representative live-cell confocal micrograph of PicoGreen staining of mt-nucleoids in control and NaBt-treated PC12-ND6 cells transfected with the mito-RFP vector. Scale bar = 5 µm. b Quantification of Mt-DNA copy number by qPCR from control and HDACi-treated PC12-ND6 cells and normalized by ΔΔ C (t). Mean ± SEM ( n = 3; * p

    Article Snippet: Untreated and treated PC12-ND6 cells were incubated in 1 ml of serum-free medium containing 3 µl of the fluorescent DNA dye PicoGreen® (Invitrogen) for 1 h at 37 °C.

    Techniques: Expressing, Staining, Transfection, Plasmid Preparation, Real-time Polymerase Chain Reaction