dna fragments New England Biolabs Search Results


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  • 99
    Thermo Fisher 3730xl dna analyzer
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs large klenow fragment new england biolabs
    Large Klenow Fragment New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs new england biolabs epimark 5 hmc
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    New England Biolabs taq dna ligase
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1092 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna size ladder
    Dna Size Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs 100 bp dna ladder
    100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna ligase
    Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 994 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna enrichment kit
    Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna gel extraction kit
    Dna Gel Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 87/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs tridye 100 bp dna ladder
    Tridye 100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs e coli dna ligase
    E Coli Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 384 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nebuilder hifi dna assembly kit
    Nebuilder Hifi Dna Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs low molecular weight dna standards
    Low Molecular Weight Dna Standards, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs quick load 100 bp dna ladder
    Quick Load 100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs nebuilder hifi dna assembly
    Nebuilder Hifi Dna Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs vector dna
    Vector Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs orf template dna sspi
    Orf Template Dna Sspi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs ptxb1 vector
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Ptxb1 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs type dna
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Type Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs pcr amplifying dna fragments harboring ndei
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Pcr Amplifying Dna Fragments Harboring Ndei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna fragments
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Dna Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 7478 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna sample prep master mix set
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Dna Sample Prep Master Mix Set, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna fragment library preparation kit
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Dna Fragment Library Preparation Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs t4 dna polymerase
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    T4 Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs fragmented hela genomic dna
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Fragmented Hela Genomic Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs vent dna polymerase
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Vent Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3756 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bacteriophage lambda dna
    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
    Bacteriophage Lambda Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
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    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
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    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the <t>pTXB1</t> vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively
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    Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the pTXB1 vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively

    Journal: Acta Neuropathologica Communications

    Article Title: Enhancement of therapeutic potential of a naturally occurring human antibody targeting a phosphorylated Ser422 containing epitope on pathological tau

    doi: 10.1186/s40478-018-0562-9

    Figure Lengend Snippet: Inhibition of in-vitro aggregation of pS422-tau by dmCBTAU-22.1. a Schematic representation of the chemical ligation process applied to preparation pS422-tau. Ser 422 (red) is phosphorylated in the CBTAU-22.1 epitope and Ser 416 (blue) is mutated into Cys as a result of the chemical ligation process. b SDS-PAGE analysis of the ligation reaction progress. Lane 1 corresponds to the full tau1–415-Mxe-CBD multidomain protein produced in E. coli after cloning into the pTXB1 vector. For the purposes of this analysis the protein was purified via His-tag affinity chromatography. Lane 2 corresponds to the thioester product obtained by treatment of the Lane 1 product with excess MESNa. Lane 3 represents the ligation product obtained by reaction of the Lane 2 product with tau peptide CL22D-P. c Size Exclusion Chromatography profiles of pS422-tau (green) and non-phosphorylated 2N4R-tau (blue). d Western blot of pS422-tau and non-phosphorylated 2N4R-tau with dmCBTAU-22.1. e Aggregation of pS422-tau in the absence (black) or presence of dmCBTAU-27.1 (red) or dmCBTAU-22.1 (blue) as monitored continuously by ThT fluorescence. The molar ratio between pS422-tau and IgG was 1: 0.6 in both cases. Each experimental condition was tested in three independent replicates, the red triplicates and two of the blue triplicates overlap and cannot be visually distinguished. f Atomic force microscopy of pS422-tau fibrils. The two panels correspond to AFM images with sizes of 6 × 6 μm and 1.5 × 1.5 μm, respectively

    Article Snippet: Production recombinant tau fragments (tau1–415-Mxe-CBD) for ligation DNA inserts for tau fragments with suitable overlaps to the pTXB1 vector (New England Biolabs) were obtained commercially (IDT) and cloned into the expressed protein ligation vector using the IMPACT kit and associated protocols (New England Biolabs).

    Techniques: Inhibition, In Vitro, Ligation, SDS Page, Produced, Clone Assay, Plasmid Preparation, Purification, Affinity Chromatography, Size-exclusion Chromatography, Western Blot, Fluorescence, Microscopy