dna fragments New England Biolabs Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs large klenow fragment new england biolabs
    Large Klenow Fragment New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/large klenow fragment new england biolabs/product/New England Biolabs
    Average 99 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    large klenow fragment new england biolabs - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher 3730xl dna analyzer
    3730xl Dna Analyzer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46651 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3730xl dna analyzer/product/Thermo Fisher
    Average 99 stars, based on 46651 article reviews
    Price from $9.99 to $1999.99
    3730xl dna analyzer - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs fragm new england biolabs
    Fragm New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fragm new england biolabs/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fragm new england biolabs - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs nebnext dna sample prep new england biolabs
    Nebnext Dna Sample Prep New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext dna sample prep new england biolabs/product/New England Biolabs
    Average 94 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    nebnext dna sample prep new england biolabs - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext fast dna fragmentation library prep set for ion torrent new england biolabs
    Nebnext Fast Dna Fragmentation Library Prep Set For Ion Torrent New England Biolabs, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext fast dna fragmentation library prep set for ion torrent new england biolabs/product/New England Biolabs
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    nebnext fast dna fragmentation library prep set for ion torrent new england biolabs - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    96
    New England Biolabs new england biolabs epimark 5 hmc
    New England Biolabs Epimark 5 Hmc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/new england biolabs epimark 5 hmc/product/New England Biolabs
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    new england biolabs epimark 5 hmc - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    96
    New England Biolabs dna fragment
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Dna Fragment, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2677 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragment/product/New England Biolabs
    Average 96 stars, based on 2677 article reviews
    Price from $9.99 to $1999.99
    dna fragment - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    94
    New England Biolabs dna fragments
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Dna Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 11859 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/New England Biolabs
    Average 94 stars, based on 11859 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs kb dna
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Kb Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kb dna/product/New England Biolabs
    Average 99 stars, based on 174 article reviews
    Price from $9.99 to $1999.99
    kb dna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs taq dna ligase
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase/product/New England Biolabs
    Average 99 stars, based on 1648 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs nebnext dna
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Nebnext Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 230 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebnext dna/product/New England Biolabs
    Average 99 stars, based on 230 article reviews
    Price from $9.99 to $1999.99
    nebnext dna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs pxba dna
    Fig. 2. <t>Gal4-VP16</t> targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ <t>DNA.</t> ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).
    Pxba Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pxba dna/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pxba dna - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs dna ligase
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna ligase/product/New England Biolabs
    Average 99 stars, based on 1307 article reviews
    Price from $9.99 to $1999.99
    dna ligase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs dna end repair
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    Dna End Repair, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna end repair/product/New England Biolabs
    Average 99 stars, based on 189 article reviews
    Price from $9.99 to $1999.99
    dna end repair - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs control dna fragments
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    Control Dna Fragments, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control dna fragments/product/New England Biolabs
    Average 99 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    control dna fragments - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    New England Biolabs 100 bp dna ladder
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    100 Bp Dna Ladder, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 715 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/100 bp dna ladder/product/New England Biolabs
    Average 99 stars, based on 715 article reviews
    Price from $9.99 to $1999.99
    100 bp dna ladder - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs dna enrichment kit
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    Dna Enrichment Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna enrichment kit/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    dna enrichment kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    99
    New England Biolabs dna gel extraction kit
    In vitro construction of <t>ADP</t> fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp <t>DNA</t> marker.
    Dna Gel Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna gel extraction kit/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    dna gel extraction kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    Image Search Results


    Fig. 2. Gal4-VP16 targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ DNA. ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).

    Journal: The EMBO Journal

    Article Title: Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

    doi: 10.1093/emboj/19.11.2629

    Figure Lengend Snippet: Fig. 2. Gal4-VP16 targets the HAT activity of SAGA and NuA4, but not that of NuA3, to the G5E4-5S array in the presence of competitor chromatin. ( A ) Diagram of the experimental protocol. ( B ) Ethidium bromide-stained agarose gel showing the migration profiles of the chromatin used as competitor (lane 1) and the reconstituted G5E4-5S array (lane 3) under the electrophoretic conditions used in (C). Lane 2 is Hin dIII-digested λ DNA. ( C ) The reconstituted array was incubated in the absence (lanes 1, 2, 5, 6, 9 and 10) or presence (3, 4, 7, 8, 11 and 12) of Gal4-VP16, and competitor chromatin was added to even-numbered lanes as indicated. Next, the reactions were incubated with SAGA, NuA3 or NuA4 in the presence of [ 3 H]acetyl-CoA, and Gal4-VP16 was competed off by incubation with an oligonucleotide corresponding to the consensus Gal4-binding site. The samples were then separated by agarose gel electrophoresis. Finally, the gels were treated for fluorography and exposed. The arrowhead indicates the position of the 5S array (also compare with lane 3 of B).

    Article Snippet: pG5E4T, a kind gift from M.Carey , contains five consensus Gal4 DNA-binding sites upstream of the adenovirus 2 E4 minimal promoter, and was linearized with Bgl I so that the Gal4 sites are central to the DNA fragment (New England Biolabs).

    Techniques: HAT Assay, Activity Assay, Staining, Agarose Gel Electrophoresis, Migration, Incubation, Binding Assay

    Fig. 1. Targeted histone acetylation by SAGA and NuA4 is required for stimulated transcription in vitro under competitive conditions. ( A ) Construct used in the transcription experiments, showing the position of the Gal4 DNA-binding sites and the 5S rDNA repeats. The chromatin template was generated as described in Materials and methods. The filled arrow signals the initiation site and the direction of transcription, while the open arrowhead shows the position and orientation of the oligonucleotide used for RNA analysis by primer extension. ( B ) Transcription assay examining the influence of targeted histone acetylation on transcription. The G5E4-5S nucleosomal array was transcribed following HAT reactions including (+) or omitting (–) Gal4-VP16, the G5E4-5S array and Superose 6-purified HAT complexes as indicated. All HAT reactions contained acetyl-CoA, a 50-fold molar excess of purified chromatin relative to the G5E4-5S array and an HIV-1 plasmid as an internal control for recovery. As indicated in the top diagram, acetyl-CoA was removed from the reaction after the acetylation step and before the transcription step. In lanes 3–10, spin column eluates lacking either Gal4-VP16 (lanes 4 and 8), the G5E4-5S array (lanes 5 and 9) or HAT complexes (lanes 6 and 10) were supplemented with the omitted component, so that transcription was performed under constant conditions. ( C ) The G5E4-5S nucleosomal array was transcribed following HAT reactions in the presence (+) or absence (–) of competitor chromatin and the NuA3 complex as indicated. All lanes contained Gal4-VP16. As in (B), the HIV-1 plasmid was included in the reaction as a recovery control. ( D ) The Superose 6 fractions of partially purified SAGA (lane 2), NuA3 (lane 3) and NuA4 (lane 4) were tested for their ability to acetylate nucleosomal histones. The samples were separated by SDS–PAGE, and the gel was stained with Coomassie Blue to determine the position of the core histones (top panel) and treated for fluorography to reveal the acetylation pattern (lower panel).

    Journal: The EMBO Journal

    Article Title: Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes

    doi: 10.1093/emboj/19.11.2629

    Figure Lengend Snippet: Fig. 1. Targeted histone acetylation by SAGA and NuA4 is required for stimulated transcription in vitro under competitive conditions. ( A ) Construct used in the transcription experiments, showing the position of the Gal4 DNA-binding sites and the 5S rDNA repeats. The chromatin template was generated as described in Materials and methods. The filled arrow signals the initiation site and the direction of transcription, while the open arrowhead shows the position and orientation of the oligonucleotide used for RNA analysis by primer extension. ( B ) Transcription assay examining the influence of targeted histone acetylation on transcription. The G5E4-5S nucleosomal array was transcribed following HAT reactions including (+) or omitting (–) Gal4-VP16, the G5E4-5S array and Superose 6-purified HAT complexes as indicated. All HAT reactions contained acetyl-CoA, a 50-fold molar excess of purified chromatin relative to the G5E4-5S array and an HIV-1 plasmid as an internal control for recovery. As indicated in the top diagram, acetyl-CoA was removed from the reaction after the acetylation step and before the transcription step. In lanes 3–10, spin column eluates lacking either Gal4-VP16 (lanes 4 and 8), the G5E4-5S array (lanes 5 and 9) or HAT complexes (lanes 6 and 10) were supplemented with the omitted component, so that transcription was performed under constant conditions. ( C ) The G5E4-5S nucleosomal array was transcribed following HAT reactions in the presence (+) or absence (–) of competitor chromatin and the NuA3 complex as indicated. All lanes contained Gal4-VP16. As in (B), the HIV-1 plasmid was included in the reaction as a recovery control. ( D ) The Superose 6 fractions of partially purified SAGA (lane 2), NuA3 (lane 3) and NuA4 (lane 4) were tested for their ability to acetylate nucleosomal histones. The samples were separated by SDS–PAGE, and the gel was stained with Coomassie Blue to determine the position of the core histones (top panel) and treated for fluorography to reveal the acetylation pattern (lower panel).

    Article Snippet: pG5E4T, a kind gift from M.Carey , contains five consensus Gal4 DNA-binding sites upstream of the adenovirus 2 E4 minimal promoter, and was linearized with Bgl I so that the Gal4 sites are central to the DNA fragment (New England Biolabs).

    Techniques: In Vitro, Construct, Binding Assay, Generated, HAT Assay, Purification, Plasmid Preparation, SDS Page, Staining

    In vitro construction of ADP fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp DNA marker.

    Journal: International Journal of Molecular Sciences

    Article Title: A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli

    doi: 10.3390/ijms13033549

    Figure Lengend Snippet: In vitro construction of ADP fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp DNA marker.

    Article Snippet: The ligation mixture was prepared by adding digested vector and digested ADP fragment with DNA ligase and its suitable ligation buffer (New England Biolabs, UK).

    Techniques: In Vitro, Polymerase Chain Reaction, Amplification, Purification, Marker