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  • 86
    Qiagen dna fragments
    Recovery of <t>DNA</t> and <t>RNA</t> from aggregates. DNA and RNA were extracted and quantified from insoluble aggregates, isolated from hippocampi of AMC (APOE ε3/ε3), or AD (ε3/ε3 or ε4/ε4) individuals (A, each N = 3); or from similar mixes of APOE ε3/ε3 and ε3/ε4 individuals (B, each N = 5–6). (C, D) DNA and RNA were extracted from insoluble aggregates from T98G glioma cells. C, independent cultures of T98G cells overexpressing APOE ε3 from a transgene were compared to cultures expressing ε4. D, T98G cells without any transgene were lysed in 0.5% NP40, and nuclei separated from cytoplasm. DNA was chiefly associated with nuclear aggregates, and RNA with cytoplasmic aggregates, as expected. Means ±SDs are shown. p values reflect 2‐tailed heteroscedastic t tests.
    Dna Fragments, supplied by Qiagen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/Qiagen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2021-07
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    86
    Roche dna fragmentation
    Impact of HO-1 siRNA on effects of SB202190 on metabolic activity, <t>apoptosis</t> and autophagy HUVEC were transfected with HO-1-specific siRNA or non-targeting siRNA (NON) 24 h prior to stimulation with SB202190 (10 μM). Following a 24-h incubation, cells were analysed for metabolic activity (A) and <t>DNA</t> fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 13–16 (A), n = 18–20 (B) or n = 20 (C, D) experiments. * P
    Dna Fragmentation, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragmentation/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragmentation - by Bioz Stars, 2021-07
    86/100 stars
      Buy from Supplier

    93
    Bio-Rad dna fragments
    Impact of HO-1 siRNA on effects of SB202190 on metabolic activity, <t>apoptosis</t> and autophagy HUVEC were transfected with HO-1-specific siRNA or non-targeting siRNA (NON) 24 h prior to stimulation with SB202190 (10 μM). Following a 24-h incubation, cells were analysed for metabolic activity (A) and <t>DNA</t> fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 13–16 (A), n = 18–20 (B) or n = 20 (C, D) experiments. * P
    Dna Fragments, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2021-07
    93/100 stars
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    99
    Millipore dna fragments
    Impact of HO-1 siRNA on effects of SB202190 on metabolic activity, <t>apoptosis</t> and autophagy HUVEC were transfected with HO-1-specific siRNA or non-targeting siRNA (NON) 24 h prior to stimulation with SB202190 (10 μM). Following a 24-h incubation, cells were analysed for metabolic activity (A) and <t>DNA</t> fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 13–16 (A), n = 18–20 (B) or n = 20 (C, D) experiments. * P
    Dna Fragments, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragments/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragments - by Bioz Stars, 2021-07
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    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
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    N/A
    The GeneArt Gene Synthesis service offers chemical synthesis cloning and sequence verification of virtually any desired genetic sequence
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    N/A
    To recover or concentrate DNA fragments 40 200 bp from agarose gel PCR or other enzymatic reactions
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    N/A
    To recover or concentrate DNA fragments 70 bp 20 kb from agarose gel PCR or other enzymatic reactions in one convenient product
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    Image Search Results


    Recovery of DNA and RNA from aggregates. DNA and RNA were extracted and quantified from insoluble aggregates, isolated from hippocampi of AMC (APOE ε3/ε3), or AD (ε3/ε3 or ε4/ε4) individuals (A, each N = 3); or from similar mixes of APOE ε3/ε3 and ε3/ε4 individuals (B, each N = 5–6). (C, D) DNA and RNA were extracted from insoluble aggregates from T98G glioma cells. C, independent cultures of T98G cells overexpressing APOE ε3 from a transgene were compared to cultures expressing ε4. D, T98G cells without any transgene were lysed in 0.5% NP40, and nuclei separated from cytoplasm. DNA was chiefly associated with nuclear aggregates, and RNA with cytoplasmic aggregates, as expected. Means ±SDs are shown. p values reflect 2‐tailed heteroscedastic t tests.

    Journal: Aging Cell

    Article Title: “Protein aggregates” contain RNA and DNA, entrapped by misfolded proteins but largely rescued by slowing translational elongation

    doi: 10.1111/acel.13326

    Figure Lengend Snippet: Recovery of DNA and RNA from aggregates. DNA and RNA were extracted and quantified from insoluble aggregates, isolated from hippocampi of AMC (APOE ε3/ε3), or AD (ε3/ε3 or ε4/ε4) individuals (A, each N = 3); or from similar mixes of APOE ε3/ε3 and ε3/ε4 individuals (B, each N = 5–6). (C, D) DNA and RNA were extracted from insoluble aggregates from T98G glioma cells. C, independent cultures of T98G cells overexpressing APOE ε3 from a transgene were compared to cultures expressing ε4. D, T98G cells without any transgene were lysed in 0.5% NP40, and nuclei separated from cytoplasm. DNA was chiefly associated with nuclear aggregates, and RNA with cytoplasmic aggregates, as expected. Means ±SDs are shown. p values reflect 2‐tailed heteroscedastic t tests.

    Article Snippet: These consisted of (1) separation of RNA and DNA fragments using a Qiagen AllPrep DNA/RNA extraction kit according to the manufacturer's protocol, with recovery assayed by absorbance at 260 nm; (2) separation of RNA and DNA fragments with the Qiagen kit, and quantitation by ethidium bromide and/or SYBR Gold after resolution by acrylamide gel electrophoresis; (3) selective enzymatic digestion with RNAse‐free DNAse (Thermo Fisher, CA) and assay by 260‐nm absorption (RNA directly; DNA by subtraction), and (4) using TRI Reagent (Molecular Research Ctr., TR118) to isolate RNA, DNA, and protein in a single protocol.

    Techniques: Isolation, Expressing

    Effects of EEF2 knockdown on the composition of aggregates in SY5Y‐APP Sw cells. Results shown in each panel comprise data from 3 independent cell expansions treated with shRNA constructs targeting EEF2, or 2 scrambled RNAs for controls. Replicate experiments produced similar results. (A, B), Western‐blot quantitation of EEF2 knockdown efficacy, evaluated by EEF2 protein; efficacies of individual shRNAs (constructs a, b, c in Methods) are superimposed in B. (C, D), total RNA fragments in aggregates, quantified by gel staining with SYBR Gold. (E, F) Total DNA fragments in aggregates, quantified by ethidium bromide fluorescence. (G, H) Total aggregate protein, quantified by staining with SYPRO Ruby. p values shown here are based on 2‐tailed heteroscedastic t tests, for 3 – 4 experiments, combining data from shRNAs a – c

    Journal: Aging Cell

    Article Title: “Protein aggregates” contain RNA and DNA, entrapped by misfolded proteins but largely rescued by slowing translational elongation

    doi: 10.1111/acel.13326

    Figure Lengend Snippet: Effects of EEF2 knockdown on the composition of aggregates in SY5Y‐APP Sw cells. Results shown in each panel comprise data from 3 independent cell expansions treated with shRNA constructs targeting EEF2, or 2 scrambled RNAs for controls. Replicate experiments produced similar results. (A, B), Western‐blot quantitation of EEF2 knockdown efficacy, evaluated by EEF2 protein; efficacies of individual shRNAs (constructs a, b, c in Methods) are superimposed in B. (C, D), total RNA fragments in aggregates, quantified by gel staining with SYBR Gold. (E, F) Total DNA fragments in aggregates, quantified by ethidium bromide fluorescence. (G, H) Total aggregate protein, quantified by staining with SYPRO Ruby. p values shown here are based on 2‐tailed heteroscedastic t tests, for 3 – 4 experiments, combining data from shRNAs a – c

    Article Snippet: These consisted of (1) separation of RNA and DNA fragments using a Qiagen AllPrep DNA/RNA extraction kit according to the manufacturer's protocol, with recovery assayed by absorbance at 260 nm; (2) separation of RNA and DNA fragments with the Qiagen kit, and quantitation by ethidium bromide and/or SYBR Gold after resolution by acrylamide gel electrophoresis; (3) selective enzymatic digestion with RNAse‐free DNAse (Thermo Fisher, CA) and assay by 260‐nm absorption (RNA directly; DNA by subtraction), and (4) using TRI Reagent (Molecular Research Ctr., TR118) to isolate RNA, DNA, and protein in a single protocol.

    Techniques: shRNA, Construct, Produced, Western Blot, Quantitation Assay, Staining, Fluorescence

    Impact of HO-1 siRNA on effects of SB202190 on metabolic activity, apoptosis and autophagy HUVEC were transfected with HO-1-specific siRNA or non-targeting siRNA (NON) 24 h prior to stimulation with SB202190 (10 μM). Following a 24-h incubation, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 13–16 (A), n = 18–20 (B) or n = 20 (C, D) experiments. * P

    Journal: Oncotarget

    Article Title: SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1

    doi: 10.18632/oncotarget.25234

    Figure Lengend Snippet: Impact of HO-1 siRNA on effects of SB202190 on metabolic activity, apoptosis and autophagy HUVEC were transfected with HO-1-specific siRNA or non-targeting siRNA (NON) 24 h prior to stimulation with SB202190 (10 μM). Following a 24-h incubation, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 13–16 (A), n = 18–20 (B) or n = 20 (C, D) experiments. * P

    Article Snippet: DNA fragmentation, an indicator of apoptosis, was determined using the Cell Death Detection ELISAplus kit (Roche Diagnostics).

    Techniques: Activity Assay, Transfection, Incubation, Expressing

    Impact of SB202190 on metabolic activity, apoptosis and cell cycle progression of HUVEC Cells were incubated with increasing concentrations of SB202190 or vehicle control for 24 h (A, C, E) or with 10 μM SB202190 for the indicated times (B, D) . Following incubation, cells were analysed for metabolic activity using WST-1 colorimetric assay (A, B), DNA fragmentation (C, D) or cell cycle distribution using flow cytometry (E). Exemplary images of flow cytometry analysis are shown for vehicle control and 10 μM SB202190, respectively (F) . Percent control represents comparison with the respective vehicle-treated time-matched group. Values are means ± SEM of n = 15–18 (A), n = 19–22 (B), n = 7–8 (C), n = 11–12 (D) and n = 3 (E) experiments. * P

    Journal: Oncotarget

    Article Title: SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1

    doi: 10.18632/oncotarget.25234

    Figure Lengend Snippet: Impact of SB202190 on metabolic activity, apoptosis and cell cycle progression of HUVEC Cells were incubated with increasing concentrations of SB202190 or vehicle control for 24 h (A, C, E) or with 10 μM SB202190 for the indicated times (B, D) . Following incubation, cells were analysed for metabolic activity using WST-1 colorimetric assay (A, B), DNA fragmentation (C, D) or cell cycle distribution using flow cytometry (E). Exemplary images of flow cytometry analysis are shown for vehicle control and 10 μM SB202190, respectively (F) . Percent control represents comparison with the respective vehicle-treated time-matched group. Values are means ± SEM of n = 15–18 (A), n = 19–22 (B), n = 7–8 (C), n = 11–12 (D) and n = 3 (E) experiments. * P

    Article Snippet: DNA fragmentation, an indicator of apoptosis, was determined using the Cell Death Detection ELISAplus kit (Roche Diagnostics).

    Techniques: Activity Assay, Incubation, Colorimetric Assay, Flow Cytometry, Cytometry

    Impact of the HO-1 inhibitor SnPPIX on effects of SB202190 on metabolic activity, apoptosis and autophagy HUVEC were pre-incubated with the HO-1 activity inhibitor SnPPIX (25 μM) for 1 h followed by addition of SB202190 (10 μM) and continuation of incubation for another 24 h. Thereafter, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 21–24 (A), n = 10–12 (B), n = 12 (C) or n = 16 (D) experiments. * P

    Journal: Oncotarget

    Article Title: SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1

    doi: 10.18632/oncotarget.25234

    Figure Lengend Snippet: Impact of the HO-1 inhibitor SnPPIX on effects of SB202190 on metabolic activity, apoptosis and autophagy HUVEC were pre-incubated with the HO-1 activity inhibitor SnPPIX (25 μM) for 1 h followed by addition of SB202190 (10 μM) and continuation of incubation for another 24 h. Thereafter, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for autophagy-related protein LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 21–24 (A), n = 10–12 (B), n = 12 (C) or n = 16 (D) experiments. * P

    Article Snippet: DNA fragmentation, an indicator of apoptosis, was determined using the Cell Death Detection ELISAplus kit (Roche Diagnostics).

    Techniques: Activity Assay, Incubation, Expressing

    Impact of the autophagy inhibitor bafilomycin A 1 on effects of SB202190 on metabolic activity, apoptosis, autophagy and HO-1 expression HUVEC were pre-incubated with the autophagy inhibitor bafilomycin A 1 (2.5 nM) for 1 h followed by addition of SB202190 (10 μM) and continuation of incubation for another 24 h. Thereafter, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for protein expression of LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 19–25 (A), n = 14–15 (B), n = 11 (C) and n = 12 (D) experiments. * P

    Journal: Oncotarget

    Article Title: SB202190 inhibits endothelial cell apoptosis via induction of autophagy and heme oxygenase-1

    doi: 10.18632/oncotarget.25234

    Figure Lengend Snippet: Impact of the autophagy inhibitor bafilomycin A 1 on effects of SB202190 on metabolic activity, apoptosis, autophagy and HO-1 expression HUVEC were pre-incubated with the autophagy inhibitor bafilomycin A 1 (2.5 nM) for 1 h followed by addition of SB202190 (10 μM) and continuation of incubation for another 24 h. Thereafter, cells were analysed for metabolic activity (A) and DNA fragmentation (B) . Lysates of cells were analysed for protein expression of LC3A/B-I/II (C) and HO-1 (D) . Protein expression values were normalised to β-actin. Percent control represents comparison with vehicle-treated group (set as 100%). Values are means ± SEM of n = 19–25 (A), n = 14–15 (B), n = 11 (C) and n = 12 (D) experiments. * P

    Article Snippet: DNA fragmentation, an indicator of apoptosis, was determined using the Cell Death Detection ELISAplus kit (Roche Diagnostics).

    Techniques: Activity Assay, Expressing, Incubation