Journal: Journal of Bacteriology
Article Title: Identification of Quorum-Sensing-Regulated Genes of Burkholderia cepacia
Figure Lengend Snippet: Characterization of identified clones containing putative CepR-C 8 ]) to sequences in the genome of B. cepacia J2315. For those clones that showed a high degree of identity at the DNA level (see text), a region of the genome surrounding 2 kbp of each sequence was subsequently annotated. In the majority of the cases, it was possible to identify a homologue of a gene coding for a protein of known function. In such cases, the names of the respective genes are indicated inside of the putative ORFs (represented as thick gray arrows). A black arrow represents the size, position, and orientation of the identified insert in each clone (in scale with each clone). Within some genetic regions shown, hypothetical proteins of unknown function were found (here designated ORF). Such was the case with P67 (ORF represents what was probably a lysR family transcriptional regulator from R. solanacearum ), P80 (ORF represents a 4-hydroxybenzoate octaprenyl transferase from Xanthomonas axonopodis ), P114 (ORF1 represents a hypothetical protein from B. fungorum ; ORF2 represents a probable transcriptional regulator, PA1627, from P. aeruginosa ), P111 (ORF2 represents a short chain dehydrogenase-reductase from Pseudomonas putida , KT2440; ORF1 represents a hypothetical protein, PA1829, from P. aeruginosa ), P79 (ORF represents a protein of unknown function), P103 (ORF represents a protein of unknown function), P56 (ORF1 represents a probable efflux membrane protein from Mesorhizobium loti ; ORF2 represents a probable nucleoside triphosphate pyrophosphohydrolase from Agrobacterium tumefaciens ), P57 (ORF1 represents a probable porin transmembrane protein from R. solanacearum ; ORF2 represents a putative lipoprotein from R. solanacearum ), P105 (ORF represents a hypothetical protein from R. solanacearum ), and P135 (ORF1 represents a human RNA binding protein; ORF2 represents a putative small heat shock protein from R . solanacearum ). See text for details of all other ORFs mentioned.
Article Snippet: DNA ligations between pSCR2 and the genomic restriction DNA fragments from B. cepacia (obtained using the restriction enzymes Nru I, Hae III, Hin cII, Alu I, and Sau 3AI independently) were transformed in E. coli DH5α and plated on selective medium containing 100 μg of ampicillin (Sigma-Aldrich)/ml, 20 μg of X-Gal (Sigma-Aldrich)/ml, and 100 nM C8 -HSL.
Techniques: Clone Assay, Sequencing, RNA Binding Assay