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  • 94
    Millipore dna extraction kit
    Genetic alterations within the <t>DNA</t> sequence of BSDL A. Sequences encoding BSDL were amplified from the RNA extracted from pancreatic tumor SOJ-6 cells using RT-PCR and specific primers. The PCR was performed in a personal Robocycler Gradient 96 (Stratagene). Following PCR, migration on agarose gel was performed to visualize amplified fragments. B. Amplicons were purified and their amino-acid sequences deduced after Sanger sequencing. The BSDL-WT amino-acid sequence corresponds to the 2.2 kb <t>amplicon</t> associated with the BSDL-Mut1 sequence, and the BSDL-Mut2 to that of the 1.8 kb band. Star symbol indicates the identical amino-acids. Only the C-terminal sequences are shown.
    Dna Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction kit/product/Millipore
    Average 94 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    dna extraction kit - by Bioz Stars, 2020-08
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    99
    Millipore dna gel extraction kit
    Schematic of small <t>DNA</t> fragment <t>(SDF)</t> generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10
    Dna Gel Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2010 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna gel extraction kit/product/Millipore
    Average 99 stars, based on 2010 article reviews
    Price from $9.99 to $1999.99
    dna gel extraction kit - by Bioz Stars, 2020-08
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    99
    Millipore bacterial dna extraction kit
    Schematic of small <t>DNA</t> fragment <t>(SDF)</t> generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10
    Bacterial Dna Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial dna extraction kit/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    bacterial dna extraction kit - by Bioz Stars, 2020-08
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    95
    Millipore genomic dna extraction kit
    The binding activities of cecropin A2 to bacterial LPS and <t>DNA.</t> (A) The affinities of cecropin A2 and control reagents to P. <t>aeruginosa</t> LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.
    Genomic Dna Extraction Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 72 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna extraction kit/product/Millipore
    Average 95 stars, based on 72 article reviews
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    99
    Qiagen deoxyribonucleic acid dna extraction kit
    The binding activities of cecropin A2 to bacterial LPS and <t>DNA.</t> (A) The affinities of cecropin A2 and control reagents to P. <t>aeruginosa</t> LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.
    Deoxyribonucleic Acid Dna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deoxyribonucleic acid dna extraction kit/product/Qiagen
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
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    94
    tiangen biotech co blood genomic deoxyribonucleic acid dna extraction kit
    The binding activities of cecropin A2 to bacterial LPS and <t>DNA.</t> (A) The affinities of cecropin A2 and control reagents to P. <t>aeruginosa</t> LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.
    Blood Genomic Deoxyribonucleic Acid Dna Extraction Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blood genomic deoxyribonucleic acid dna extraction kit/product/tiangen biotech co
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    blood genomic deoxyribonucleic acid dna extraction kit - by Bioz Stars, 2020-08
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    99
    Millipore dna extraction special kits
    The binding activities of cecropin A2 to bacterial LPS and <t>DNA.</t> (A) The affinities of cecropin A2 and control reagents to P. <t>aeruginosa</t> LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.
    Dna Extraction Special Kits, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction special kits/product/Millipore
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    dna extraction special kits - by Bioz Stars, 2020-08
    99/100 stars
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    Image Search Results


    Genetic alterations within the DNA sequence of BSDL A. Sequences encoding BSDL were amplified from the RNA extracted from pancreatic tumor SOJ-6 cells using RT-PCR and specific primers. The PCR was performed in a personal Robocycler Gradient 96 (Stratagene). Following PCR, migration on agarose gel was performed to visualize amplified fragments. B. Amplicons were purified and their amino-acid sequences deduced after Sanger sequencing. The BSDL-WT amino-acid sequence corresponds to the 2.2 kb amplicon associated with the BSDL-Mut1 sequence, and the BSDL-Mut2 to that of the 1.8 kb band. Star symbol indicates the identical amino-acids. Only the C-terminal sequences are shown.

    Journal: Oncotarget

    Article Title: Expression of truncated bile salt-dependent lipase variant in pancreatic pre-neoplastic lesions

    doi: 10.18632/oncotarget.11777

    Figure Lengend Snippet: Genetic alterations within the DNA sequence of BSDL A. Sequences encoding BSDL were amplified from the RNA extracted from pancreatic tumor SOJ-6 cells using RT-PCR and specific primers. The PCR was performed in a personal Robocycler Gradient 96 (Stratagene). Following PCR, migration on agarose gel was performed to visualize amplified fragments. B. Amplicons were purified and their amino-acid sequences deduced after Sanger sequencing. The BSDL-WT amino-acid sequence corresponds to the 2.2 kb amplicon associated with the BSDL-Mut1 sequence, and the BSDL-Mut2 to that of the 1.8 kb band. Star symbol indicates the identical amino-acids. Only the C-terminal sequences are shown.

    Article Snippet: Amplicon purification and sequencing The amplicons obtained were then purified using the DNA extraction kit (Millipore) after electrophoretic migration on agarose gel, according to the protocol recommended by the supplier.

    Techniques: Sequencing, Amplification, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Migration, Agarose Gel Electrophoresis, Purification

    Schematic of small DNA fragment (SDF) generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10

    Journal:

    Article Title: CFTR gene targeting in mouse embryonic stem cells mediated by Small Fragment Homologous Replacement (SFHR)

    doi:

    Figure Lengend Snippet: Schematic of small DNA fragment (SDF) generation and PCR analysis of SFHR A. SDF (783bp) containing the ΔF508 mutation and a KpnI restriction enzyme cleavage site was synthesized using primers mCF1 and mCF15, localized within introns 9 and 10

    Article Snippet: Before transfection the SDF was used, always gel and ethanol purified (DNA gel extraction kit; Millipore, Bedford, MA).

    Techniques: Polymerase Chain Reaction, Mutagenesis, Synthesized

    Microscopic analysis of the Helicobacter pullorum positive Meckel´s diverticulum case. Low power view of a histological section from Meckel´s diverticulum, positive for Helicobacter pullorum ( H. pullorum ) DNA, in the heterotopic area. There is an unusually well developed lymphatic tissue with germinal centres and a predominance of lymphocytes in the non-heterotopic area. Plasma cells were mainly found in the heterotopic area. The small arrows in the big square, point to a small strip of gastric heterotopia. This area displays at higher magnification in the inset. Large arrows point to gastric glands of corpus type. Blue without arrows in the photo indicates intestinal mucus. Alcian blue-PAS staining pH 2.5.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Helicobacter species and gut bacterial DNA in Meckel's diverticulum and the appendix

    doi: 10.3748/wjg.v17.i36.4104

    Figure Lengend Snippet: Microscopic analysis of the Helicobacter pullorum positive Meckel´s diverticulum case. Low power view of a histological section from Meckel´s diverticulum, positive for Helicobacter pullorum ( H. pullorum ) DNA, in the heterotopic area. There is an unusually well developed lymphatic tissue with germinal centres and a predominance of lymphocytes in the non-heterotopic area. Plasma cells were mainly found in the heterotopic area. The small arrows in the big square, point to a small strip of gastric heterotopia. This area displays at higher magnification in the inset. Large arrows point to gastric glands of corpus type. Blue without arrows in the photo indicates intestinal mucus. Alcian blue-PAS staining pH 2.5.

    Article Snippet: Helicobacter specific PCR products were purified from agarose gels using the Montage DNA Gel Extraction Kit (Millipore, Bedford, MA, United States), according to the manufacturer’s instructions.

    Techniques: Stripping Membranes, Staining

    FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced DNA fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P

    Journal: Annals of Surgery

    Article Title: Trauma-Hemorrhagic Shock Mesenteric Lymph Induces Endothelial Apoptosis That Involves Both Caspase-Dependent and Caspase-Independent Mechanisms

    doi: 10.1097/01.sla.0000129341.94219.cf

    Figure Lengend Snippet: FIGURE 2. (A) T/HS lymph but not T/SS lymph or medium induced DNA fragmentation. HUVECs were incubated with lymph for 4 hours, after which genomic DNA was isolated, fractionated on a 1.5% agarose gel and stained with ethidium bromide. The hallmark DNA step-laddering pattern characteristic of apoptosis is present only in the HUVECS incubated with T/HS lymph. These results are representative of 3 independent studies (M, marker). (B) The number of free nucleosomes generated in HUVECs after 4-hour incubation with T/HS lymph was increased compared with HUVECs incubated in medium or T/SS lymph. Nuclear extracts were prepared using a Nucleosome ELISA kit (Oncogene Research). Optical density (OD) correlates with the number of free nucleosomes. Data expressed as mean ± SD. * P

    Article Snippet: Using a DNA Isolation Kit (Oncogene Research Products, Boston MA) low molecular weight DNA extracts were prepared.

    Techniques: Incubation, Isolation, Agarose Gel Electrophoresis, Staining, Marker, Generated, Enzyme-linked Immunosorbent Assay

    The binding activities of cecropin A2 to bacterial LPS and DNA. (A) The affinities of cecropin A2 and control reagents to P. aeruginosa LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Synergistic Efficacy of Aedes aegypti Antimicrobial Peptide Cecropin A2 and Tetracycline against Pseudomonas aeruginosa

    doi: 10.1128/AAC.00686-17

    Figure Lengend Snippet: The binding activities of cecropin A2 to bacterial LPS and DNA. (A) The affinities of cecropin A2 and control reagents to P. aeruginosa LPS were determined using the BODIPY TR cadaverine displacement method. (B) Interaction of cecropin A2 and control reagents with P. aeruginosa genomic DNA. To highlight the effects of high concentrations of cecropin A2 on its DNA-binding activity, we ran multiple gels that demonstrated a decreasing intensity of DNA bands from cells that were treated with a high concentration of the peptide (representative bands from two different gels with complete pictures of the gels included in Fig. S1 in the supplemental material). (C) The DNA-binding ability of cecropin A2. DNA bands were quantified with ImageJ software and DNA binding (%) was defined as the ratio of nonmigrating DNA to total genomic DNA.

    Article Snippet: P. aeruginosa strain PA14 genomic DNA was extracted using the bacterial genomic DNA extraction kit (Sigma-Aldrich Biotechnology, St. Louis, MO), and 20 μl of the purified genomic DNA was incubated with cecropin A2, LL-37, or tetracycline for 1 h at room temperature.

    Techniques: Binding Assay, Activity Assay, Concentration Assay, Software