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    Thermo Fisher dna content
    Dna Content, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore dna content
    Dna Content, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson dna content analysis
    Transduction of serum-deprived cultures. Normal human fibroblasts were grown to confluence and serum starved for 4 days (Stationary) or were serum-starved for 3 days and then plated in medium with 10% serum 16 h prior to the addition of vector (Dividing). (A) Flow cytometry analysis of the <t>DNA</t> content by <t>propidium</t> iodide staining. (B) The number of AP + foci in stationary cultures divided by the number of AP + foci in dividing cultures (S/D ratio) is reported for three independent experiments with standard errors (error bars). HIV vector preparations were prepared with (HIV+Acc) and without (HIV−Acc) the accessory genes vif , vpr , vpu , and nef . An asterisk indicates significant differences ( P
    Dna Content Analysis, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson dna content
    CypA is not required for Vpr-induced cell cycle arrest in the context of HIV-1 infection. Vpr was introduced by infection with replication-competent HIV-1 NL 4 - 3 . The isogenic, Vpr-negative mutant, HIV-1 NL 4 - 3 vprX, was used as a negative control. Infections of CypA + and CypA −/− cells were analyzed at 4 (A) and 7 (B) days postinfection by simultaneous intracellular p24 and <t>DNA</t> content staining with propidium iodide. The p24-positive (infected) and -negative (uninfected) cells from the cultures were analyzed by flow <t>cytometry</t> for DNA content after being gated based on p24 status. Relative G 1 , S, and G 2 /M distribution as well as infection rates are indicated.
    Dna Content, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Transduction of serum-deprived cultures. Normal human fibroblasts were grown to confluence and serum starved for 4 days (Stationary) or were serum-starved for 3 days and then plated in medium with 10% serum 16 h prior to the addition of vector (Dividing). (A) Flow cytometry analysis of the DNA content by propidium iodide staining. (B) The number of AP + foci in stationary cultures divided by the number of AP + foci in dividing cultures (S/D ratio) is reported for three independent experiments with standard errors (error bars). HIV vector preparations were prepared with (HIV+Acc) and without (HIV−Acc) the accessory genes vif , vpr , vpu , and nef . An asterisk indicates significant differences ( P

    Journal: Journal of Virology

    Article Title: Cell Cycle Requirements for Transduction by Foamy Virus Vectors Compared to Those of Oncovirus and Lentivirus Vectors

    doi: 10.1128/JVI.78.5.2327-2335.2004

    Figure Lengend Snippet: Transduction of serum-deprived cultures. Normal human fibroblasts were grown to confluence and serum starved for 4 days (Stationary) or were serum-starved for 3 days and then plated in medium with 10% serum 16 h prior to the addition of vector (Dividing). (A) Flow cytometry analysis of the DNA content by propidium iodide staining. (B) The number of AP + foci in stationary cultures divided by the number of AP + foci in dividing cultures (S/D ratio) is reported for three independent experiments with standard errors (error bars). HIV vector preparations were prepared with (HIV+Acc) and without (HIV−Acc) the accessory genes vif , vpr , vpu , and nef . An asterisk indicates significant differences ( P

    Article Snippet: For DNA content analysis, cells were stained with propidium iodide as described previously ( ) and analyzed by flow cytometry using a Becton Dickinson Facstar.

    Techniques: Transduction, Plasmid Preparation, Flow Cytometry, Cytometry, Staining

    CypA is not required for Vpr-induced cell cycle arrest in the context of HIV-1 infection. Vpr was introduced by infection with replication-competent HIV-1 NL 4 - 3 . The isogenic, Vpr-negative mutant, HIV-1 NL 4 - 3 vprX, was used as a negative control. Infections of CypA + and CypA −/− cells were analyzed at 4 (A) and 7 (B) days postinfection by simultaneous intracellular p24 and DNA content staining with propidium iodide. The p24-positive (infected) and -negative (uninfected) cells from the cultures were analyzed by flow cytometry for DNA content after being gated based on p24 status. Relative G 1 , S, and G 2 /M distribution as well as infection rates are indicated.

    Journal: Journal of Virology

    Article Title: Induction of G2 Arrest and Binding to Cyclophilin A Are Independent Phenotypes of Human Immunodeficiency Virus Type 1 Vpr

    doi: 10.1128/JVI.80.8.3694-3700.2006

    Figure Lengend Snippet: CypA is not required for Vpr-induced cell cycle arrest in the context of HIV-1 infection. Vpr was introduced by infection with replication-competent HIV-1 NL 4 - 3 . The isogenic, Vpr-negative mutant, HIV-1 NL 4 - 3 vprX, was used as a negative control. Infections of CypA + and CypA −/− cells were analyzed at 4 (A) and 7 (B) days postinfection by simultaneous intracellular p24 and DNA content staining with propidium iodide. The p24-positive (infected) and -negative (uninfected) cells from the cultures were analyzed by flow cytometry for DNA content after being gated based on p24 status. Relative G 1 , S, and G 2 /M distribution as well as infection rates are indicated.

    Article Snippet: Cells were washed again with FACS buffer, incubated in DNA staining buffer (10 μg of propidium iodide/ml and 11.25 kU of RNase A/ml in FACS buffer) for 15 min, and analyzed by FACScan flow cytometry for GFP expression or DNA content (Beckton Dickinson, Franklin Lakes, N.J.).

    Techniques: Infection, Mutagenesis, Negative Control, Staining, Flow Cytometry, Cytometry

    CypA is not required for Vpr-mediated cell cycle arrest. (A) Cell cycle analysis of CypA + and CypA −/− Jurkat cells infected with pHR-VPR, encoding Vpr and GFP, or the control vector, pHR-GFP, encoding GFP only. Vector-infected cells were analyzed 24 h postinfection for DNA content and, separately, for GFP expression, using flow cytometry with the Modfit software package (Verity Software House, Inc., Topsham, Maine). Relative G 1 , S, and G 2 /M distributions as well as infection rates are indicated. “Percent infected” indicates the percentage of GFP-positive cells. The results are representative of two different experiments. (B) CypA expression in Jurkat cells. Lysates of CypA −/− and CypA + were subjected to Western blot analysis with anti-CypA antibody to verify the lack of CypA expression in CypA −/− cells.

    Journal: Journal of Virology

    Article Title: Induction of G2 Arrest and Binding to Cyclophilin A Are Independent Phenotypes of Human Immunodeficiency Virus Type 1 Vpr

    doi: 10.1128/JVI.80.8.3694-3700.2006

    Figure Lengend Snippet: CypA is not required for Vpr-mediated cell cycle arrest. (A) Cell cycle analysis of CypA + and CypA −/− Jurkat cells infected with pHR-VPR, encoding Vpr and GFP, or the control vector, pHR-GFP, encoding GFP only. Vector-infected cells were analyzed 24 h postinfection for DNA content and, separately, for GFP expression, using flow cytometry with the Modfit software package (Verity Software House, Inc., Topsham, Maine). Relative G 1 , S, and G 2 /M distributions as well as infection rates are indicated. “Percent infected” indicates the percentage of GFP-positive cells. The results are representative of two different experiments. (B) CypA expression in Jurkat cells. Lysates of CypA −/− and CypA + were subjected to Western blot analysis with anti-CypA antibody to verify the lack of CypA expression in CypA −/− cells.

    Article Snippet: Cells were washed again with FACS buffer, incubated in DNA staining buffer (10 μg of propidium iodide/ml and 11.25 kU of RNase A/ml in FACS buffer) for 15 min, and analyzed by FACScan flow cytometry for GFP expression or DNA content (Beckton Dickinson, Franklin Lakes, N.J.).

    Techniques: Cell Cycle Assay, Infection, Plasmid Preparation, Expressing, Flow Cytometry, Cytometry, Software, Western Blot

    Induction of G 2 arrest by Vpr and Vpr(P35N) and failure of CsA to inhibit Vpr function. CypA + (A) or CypA −/− (B) Jurkat cells were mock infected or infected with pHR-GFP, pHR-VPR, or pHR-VPR(P35N) and then subdivided into two cultures, which were incubated in the presence or absence of 2.5 μM CsA, respectively. Cells were analyzed 48 h postinfection for DNA content and, separately, for GFP expression using flow cytometry. Relative G 1 , S, and G 2 /M distributions as well as infection rates are indicated.

    Journal: Journal of Virology

    Article Title: Induction of G2 Arrest and Binding to Cyclophilin A Are Independent Phenotypes of Human Immunodeficiency Virus Type 1 Vpr

    doi: 10.1128/JVI.80.8.3694-3700.2006

    Figure Lengend Snippet: Induction of G 2 arrest by Vpr and Vpr(P35N) and failure of CsA to inhibit Vpr function. CypA + (A) or CypA −/− (B) Jurkat cells were mock infected or infected with pHR-GFP, pHR-VPR, or pHR-VPR(P35N) and then subdivided into two cultures, which were incubated in the presence or absence of 2.5 μM CsA, respectively. Cells were analyzed 48 h postinfection for DNA content and, separately, for GFP expression using flow cytometry. Relative G 1 , S, and G 2 /M distributions as well as infection rates are indicated.

    Article Snippet: Cells were washed again with FACS buffer, incubated in DNA staining buffer (10 μg of propidium iodide/ml and 11.25 kU of RNase A/ml in FACS buffer) for 15 min, and analyzed by FACScan flow cytometry for GFP expression or DNA content (Beckton Dickinson, Franklin Lakes, N.J.).

    Techniques: Infection, Incubation, Expressing, Flow Cytometry, Cytometry