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  • 99
    Millipore dna bands
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Dna Bands, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 241 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna bands
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Dna Bands, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1259 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad dna bands size
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Dna Bands Size, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare dna band purification kit
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Dna Band Purification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Omega Bio-tek dna band purification kit
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Dna Band Purification Kit, supplied by Omega Bio-tek, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare gel band dna purification kit
    Polyacrylamide gel electrophoresis of the <t>Sigma</t> C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The <t>DNA</t> molecular weight markers are indicated on the left.
    Gel Band Dna Purification Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare dna band
    Discovery of <t>DNA</t> sequences with urea-resistant structures. ( a ) The sequence of the DNA library used for in vitro selection. R: adenosine ribonucleotide; F: fluorescein-labeled dT; Q: dabcyl-labeled dT; N: mixture of ACGT (25% each). ( b ) The random-sequence regions of UD1, UD2 and UD3. ( c ) Fluorimage and phosphorimage of a <t>7MU-dPAGE</t> gel conducted to analyze the cleavage reaction of UD1–3. Insert: The cleavage reaction with 14-nt P1 and 84-nt P2 as the cleavage product. L: DNA ladder lane. ( d ) 7MU-dPAGE analysis of U1T, U2T and U3T (truncated UD1, UD2 and UD3 with the removal of the first 28 nucleotides). ( e ) 7MU-dPAGE analysis of the boxed DNA bands from panel d. + M and -M: with and without 50 mM NaCl and 5 mM MgCl 2 , respectively. Cropped gel images were used in panels ( c – e ).
    Dna Band, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 245 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    IBI Scientific dna band
    Discovery of <t>DNA</t> sequences with urea-resistant structures. ( a ) The sequence of the DNA library used for in vitro selection. R: adenosine ribonucleotide; F: fluorescein-labeled dT; Q: dabcyl-labeled dT; N: mixture of ACGT (25% each). ( b ) The random-sequence regions of UD1, UD2 and UD3. ( c ) Fluorimage and phosphorimage of a <t>7MU-dPAGE</t> gel conducted to analyze the cleavage reaction of UD1–3. Insert: The cleavage reaction with 14-nt P1 and 84-nt P2 as the cleavage product. L: DNA ladder lane. ( d ) 7MU-dPAGE analysis of U1T, U2T and U3T (truncated UD1, UD2 and UD3 with the removal of the first 28 nucleotides). ( e ) 7MU-dPAGE analysis of the boxed DNA bands from panel d. + M and -M: with and without 50 mM NaCl and 5 mM MgCl 2 , respectively. Cropped gel images were used in panels ( c – e ).
    Dna Band, supplied by IBI Scientific, used in various techniques. Bioz Stars score: 90/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Illumina Inc dna band
    Discovery of <t>DNA</t> sequences with urea-resistant structures. ( a ) The sequence of the DNA library used for in vitro selection. R: adenosine ribonucleotide; F: fluorescein-labeled dT; Q: dabcyl-labeled dT; N: mixture of ACGT (25% each). ( b ) The random-sequence regions of UD1, UD2 and UD3. ( c ) Fluorimage and phosphorimage of a <t>7MU-dPAGE</t> gel conducted to analyze the cleavage reaction of UD1–3. Insert: The cleavage reaction with 14-nt P1 and 84-nt P2 as the cleavage product. L: DNA ladder lane. ( d ) 7MU-dPAGE analysis of U1T, U2T and U3T (truncated UD1, UD2 and UD3 with the removal of the first 28 nucleotides). ( e ) 7MU-dPAGE analysis of the boxed DNA bands from panel d. + M and -M: with and without 50 mM NaCl and 5 mM MgCl 2 , respectively. Cropped gel images were used in panels ( c – e ).
    Dna Band, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad dna bands
    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of <t>DNA</t> oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide <t>TBE</t> gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.
    Dna Bands, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 2114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Nippon Genetics dna bands
    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of <t>DNA</t> oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide <t>TBE</t> gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.
    Dna Bands, supplied by Nippon Genetics, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Biometra dna bands
    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of <t>DNA</t> oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide <t>TBE</t> gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.
    Dna Bands, supplied by Biometra, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa dna bands
    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of <t>DNA</t> oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide <t>TBE</t> gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.
    Dna Bands, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BGI Tech Solutions Co Ltd dna bands
    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of <t>DNA</t> oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide <t>TBE</t> gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.
    Dna Bands, supplied by BGI Tech Solutions Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Polyacrylamide gel electrophoresis of the Sigma C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The DNA molecular weight markers are indicated on the left.

    Journal: Scientific Reports

    Article Title: Phenotypic, genotypic and antigenic characterization of emerging avian reoviruses isolated from clinical cases of arthritis in broilers in Saskatchewan, Canada

    doi: 10.1038/s41598-017-02743-8

    Figure Lengend Snippet: Polyacrylamide gel electrophoresis of the Sigma C RT-PCR products (~960 bp) following digestion with the indicated restriction enzymes. The DNA molecular weight markers are indicated on the left.

    Article Snippet: As shown in Fig. , the Bcn I enzyme cutting site was only present in the sequence of the SK-R23 isolate from Cluster II (Fig. ) creating two DNA bands after digestion of the Sigma C PCR product (Fig. ).

    Techniques: Polyacrylamide Gel Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Molecular Weight

    The L1 copy number is increased in HMW genomic DNA from HIV-1-infected primary CD4 + T cells. CD4 + T cells from HIV-1-uninfected donors were either infected with 0.05 MOI of HIV-1-81A or maintained as a mock-infected control. (A) At 172 h postinfection,

    Journal: Journal of Virology

    Article Title: LINE-1 Retrotransposable Element DNA Accumulates in HIV-1-Infected Cells

    doi: 10.1128/JVI.02257-13

    Figure Lengend Snippet: The L1 copy number is increased in HMW genomic DNA from HIV-1-infected primary CD4 + T cells. CD4 + T cells from HIV-1-uninfected donors were either infected with 0.05 MOI of HIV-1-81A or maintained as a mock-infected control. (A) At 172 h postinfection,

    Article Snippet: High-molecular-weight (HMW) DNA bands were excised and purified using a Puregene gel extraction kit (Sigma).

    Techniques: Infection

    Discovery of DNA sequences with urea-resistant structures. ( a ) The sequence of the DNA library used for in vitro selection. R: adenosine ribonucleotide; F: fluorescein-labeled dT; Q: dabcyl-labeled dT; N: mixture of ACGT (25% each). ( b ) The random-sequence regions of UD1, UD2 and UD3. ( c ) Fluorimage and phosphorimage of a 7MU-dPAGE gel conducted to analyze the cleavage reaction of UD1–3. Insert: The cleavage reaction with 14-nt P1 and 84-nt P2 as the cleavage product. L: DNA ladder lane. ( d ) 7MU-dPAGE analysis of U1T, U2T and U3T (truncated UD1, UD2 and UD3 with the removal of the first 28 nucleotides). ( e ) 7MU-dPAGE analysis of the boxed DNA bands from panel d. + M and -M: with and without 50 mM NaCl and 5 mM MgCl 2 , respectively. Cropped gel images were used in panels ( c – e ).

    Journal: Scientific Reports

    Article Title: Serendipitous Discovery of a Guanine-rich DNA Molecule with a Highly Stable Structure in Urea

    doi: 10.1038/s41598-018-20248-w

    Figure Lengend Snippet: Discovery of DNA sequences with urea-resistant structures. ( a ) The sequence of the DNA library used for in vitro selection. R: adenosine ribonucleotide; F: fluorescein-labeled dT; Q: dabcyl-labeled dT; N: mixture of ACGT (25% each). ( b ) The random-sequence regions of UD1, UD2 and UD3. ( c ) Fluorimage and phosphorimage of a 7MU-dPAGE gel conducted to analyze the cleavage reaction of UD1–3. Insert: The cleavage reaction with 14-nt P1 and 84-nt P2 as the cleavage product. L: DNA ladder lane. ( d ) 7MU-dPAGE analysis of U1T, U2T and U3T (truncated UD1, UD2 and UD3 with the removal of the first 28 nucleotides). ( e ) 7MU-dPAGE analysis of the boxed DNA bands from panel d. + M and -M: with and without 50 mM NaCl and 5 mM MgCl 2 , respectively. Cropped gel images were used in panels ( c – e ).

    Article Snippet: The unfolded and folded DNA bands separated by dPAGE gel was imaged with a Typhoon Trio + Imager (GE Healthcare) and the radioactivity of each DNA band was quantified with ImageQuant software (Molecular Dynamics).

    Techniques: Sequencing, In Vitro, Selection, Labeling

    ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of DNA oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide TBE gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.

    Journal: PLoS ONE

    Article Title: Structural and Mechanistic Insight into DNA Unwinding by Deinococcus radiodurans UvrD

    doi: 10.1371/journal.pone.0077364

    Figure Lengend Snippet: ssDNA translocase activity of dr UvrD. Translocase activity of dr UvrD was assayed using the streptavidin-displacement assay. A. Structure of DNA oligonucleotides used for dr UvrD translocase assay measuring streptavidin displacement from biotinylated DNA substrates. The fluorescein label is represented as a star and the biotin label as a circle. B. Time course of dr UvrD (250 nM) catalyzed streptavidin displacement from the 3′- (blue) and 5′- (red) ssDNA extensions of DNA oligonucleotides shown in (A). The fraction of released dsDNA (no longer bound to streptavidin) was quantified and plotted as a function of time. C. Translocase activ ity of dr UvrD (250 nM) on 5' tailed dsDNA (20 nM) as a function of time in the absence (left) and the presence (right) of dr SSB (250 nM). The reaction products were analyzed on a 10 % polyacrylamide TBE gel. Bands correspond to the fluorescein labeled reaction products: streptavidin-bound dsDNA (upper bands, corresponding to several biotin labeled oligonucleotides bound to streptavidin), released dsDNA (middle band) and unwound ssDNA (lower band). D. The bands shown in (C), resulting from the time course of streptavidin displacement from 5′- tailed dsDNA, were quantified and the fraction of streptavidin-bound (black), released dsDNA (red) and unwound ssDNA (blue) were plotted as a function of time for reactions carried out in the absence (full lines) and presence (dotted lines) of dr SSB (250 nM). Standard deviations are shown as vertical bars.

    Article Snippet: Reaction products were run on a 20 % polyacrylamide TBE gel and the DNA bands were visualized and quantified using a ChemiDoc MP imaging system and the Image Lab software (Bio-Rad).

    Techniques: Activity Assay, Labeling