dna Bei Resources Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    BEI Resources haematobium dna
    Haematobium Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haematobium dna/product/BEI Resources
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    haematobium dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    BEI Resources mock community dna
    Mock Community Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mock community dna/product/BEI Resources
    Average 88 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    mock community dna - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    92
    BEI Resources dna extraction
    Dna Extraction, supplied by BEI Resources, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction/product/BEI Resources
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna extraction - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    BEI Resources processing dna
    Processing Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/processing dna/product/BEI Resources
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    processing dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    BEI Resources mock bacterial community dna
    Mock Bacterial Community Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mock bacterial community dna/product/BEI Resources
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mock bacterial community dna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    BEI Resources francisella dna
    Francisella Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/francisella dna/product/BEI Resources
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    francisella dna - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    BEI Resources dna extraction blank
    Dna Extraction Blank, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna extraction blank/product/BEI Resources
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    dna extraction blank - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    BEI Resources synthetic dna mock community
    Synthetic Dna Mock Community, supplied by BEI Resources, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/synthetic dna mock community/product/BEI Resources
    Average 88 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    synthetic dna mock community - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    93
    BEI Resources mock community dna mock communities purified dna
    Mock Community Dna Mock Communities Purified Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mock community dna mock communities purified dna/product/BEI Resources
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mock community dna mock communities purified dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    88
    BEI Resources p knowlesi dna
    P Knowlesi Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p knowlesi dna/product/BEI Resources
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    p knowlesi dna - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    93
    BEI Resources m leprae dna
    M Leprae Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m leprae dna/product/BEI Resources
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m leprae dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    93
    BEI Resources sequence specific positive control dna
    Sequence Specific Positive Control Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sequence specific positive control dna/product/BEI Resources
    Average 93 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    sequence specific positive control dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    BEI Resources s mansoni dna
    A sequence of images illustrating the plasma separation process. (A) A 200 μL of blood sample spiked with S. <t>mansoni</t> <t>DNA</t> was loaded into the superhydrophobic plasma separator. (B) When the top cover was closed, the blood was sandwiched between
    S Mansoni Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s mansoni dna/product/BEI Resources
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s mansoni dna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    BEI Resources human microbiome project mock community dna
    Overview of experimental design. During phase I, <t>DNA</t> extraction treatments were performed on subdivided tissue, with efficiency of SSU gene amplification assessed using gel screening of PCR products. The green check mark and red X indicate that amplicons from the treatment were and were not chosen for sequencing, respectively. During phase II, well-performing PB, VG, PG, and VGl extracts were amplified and sequenced for microbial community analysis. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass
    Human Microbiome Project Mock Community Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human microbiome project mock community dna/product/BEI Resources
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    human microbiome project mock community dna - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    93
    BEI Resources dna based sequence independent single primer amplification sispa
    Overview of experimental design. During phase I, <t>DNA</t> extraction treatments were performed on subdivided tissue, with efficiency of SSU gene amplification assessed using gel screening of PCR products. The green check mark and red X indicate that amplicons from the treatment were and were not chosen for sequencing, respectively. During phase II, well-performing PB, VG, PG, and VGl extracts were amplified and sequenced for microbial community analysis. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass
    Dna Based Sequence Independent Single Primer Amplification Sispa, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna based sequence independent single primer amplification sispa/product/BEI Resources
    Average 93 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    dna based sequence independent single primer amplification sispa - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    90
    BEI Resources mtb genomic dna
    Overview of experimental design. During phase I, <t>DNA</t> extraction treatments were performed on subdivided tissue, with efficiency of SSU gene amplification assessed using gel screening of PCR products. The green check mark and red X indicate that amplicons from the treatment were and were not chosen for sequencing, respectively. During phase II, well-performing PB, VG, PG, and VGl extracts were amplified and sequenced for microbial community analysis. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass
    Mtb Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtb genomic dna/product/BEI Resources
    Average 90 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    mtb genomic dna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    BEI Resources haematobium genomic dna
    Detection of dual infection (both S . mansoni and S . <t>haematobium</t> ) by species-specific repeat <t>DNA</t> amplification from single urine sample. The dual infection for both species is categorized into two different age groups (Group A: 7–12 years and Group B: 13–15 years) and for both female and male participants.
    Haematobium Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haematobium genomic dna/product/BEI Resources
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    haematobium genomic dna - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    90
    BEI Resources bacteria genomic dna
    Detection of dual infection (both S . mansoni and S . <t>haematobium</t> ) by species-specific repeat <t>DNA</t> amplification from single urine sample. The dual infection for both species is categorized into two different age groups (Group A: 7–12 years and Group B: 13–15 years) and for both female and male participants.
    Bacteria Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacteria genomic dna/product/BEI Resources
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bacteria genomic dna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    BEI Resources mle br 4923 genomic dna
    Overview of mycobacterial DnaB inteins. a Relationship of DnaB inteins in three mycobacterial species. The two M. smegmatis ( Msm ) DnaB inteins (DnaBi1 and DnaBi2) were aligned to the single DnaB inteins in M. leprae ( <t>Mle</t> ) and M. tuberculosis ( Mtu ) using a protein pairwise alignment (EMBOSS Needle; http://www.ebi.ac.uk/Tools/psa/emboss_needle ). The amino acid percent identity between inteins (red) are shown. The number of residues for the intein and extein fragments (white) are indicated and the homing endonuclease (HEN) is shown in black. The N-terminal domain of DnaB is indicated in light blue shading and the C-terminal ATPase is indicated in gray shading. NTD N-terminal domain, CTD C-terminal domain, aa amino acid. b Localization of DnaB intein insertions. A structure model of the DnaB ATPase domain (residues 200–461) is shown 54 . The insertion sites of DnaBi1 and DnaBi2 are indicated by the S + 1 in purple. The P-loop, where DnaBi1 is inserted, is cyan and the H4 <t>motif/DNA-binding</t> loop, where DnaBi2 is found, is orange. c Intein splicing mechanisms. The class 3 mechanism (top) is used by Msm DnaBi1 and Mle DnaBi, whereas the canonical class 1 pathway (bottom) is used by Msm DnaBi2 and Mtu DnaBi. See the main text and Supplementary Figure 1 for detailed splicing description and steps (boxed numbers). Residue numbering refers to Msm inteins
    Mle Br 4923 Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mle br 4923 genomic dna/product/BEI Resources
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    mle br 4923 genomic dna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    93
    BEI Resources genomic dna
    Experimental Workflow. Approximately 1 mL of saliva was collected from study participants using a commercially available kit from OMNIgene. Bacteria within the saliva samples were then vigorously lysed using both enzymatic and mechanical techniques. After purifying genomic <t>DNA,</t> amplicons from the 16S rRNA gene were generated, sequenced on an Illumina MiSeq, and analyzed using the DADA2 computational pipeline to determine <t>microbiome</t> composition.
    Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic dna/product/BEI Resources
    Average 93 stars, based on 239 article reviews
    Price from $9.99 to $1999.99
    genomic dna - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    BEI Resources plasmid dna
    Experimental Workflow. Approximately 1 mL of saliva was collected from study participants using a commercially available kit from OMNIgene. Bacteria within the saliva samples were then vigorously lysed using both enzymatic and mechanical techniques. After purifying genomic <t>DNA,</t> amplicons from the 16S rRNA gene were generated, sequenced on an Illumina MiSeq, and analyzed using the DADA2 computational pipeline to determine <t>microbiome</t> composition.
    Plasmid Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid dna/product/BEI Resources
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    plasmid dna - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    BEI Resources influenza virus genomic dna
    Hierarchical clustering of mixed samples demonstrates the resolution capabilities of the UBDA array . This dendogram and heat map illustrates a unique bio-signature pattern obtained from Lactobacillus <t>plantarum</t> , mixed sample (synthetic mixture in a 4:1 ratio of L. plantarum and Streptococcus mitis ), S. mitis , mixed sample (a synthetic mixture of L. plantarum and S. mitis genomic <t>DNA</t> in a ratio of 4:1 with a spike-in of pBluescript plasmid at 50 ng) and pBluescript plasmid. Normalized data from the 9-mer data set were filtered for intensity signals greater than the 20 th percentile. Only intensity signals with a fold change of 5 or greater were included. These 36,059 elements were subjected to hierarchical clustering with Euclidean distance being used as a similarity measure. The signal intensity values were represented on a log 2 scale and range from 8.4 to 13.4.
    Influenza Virus Genomic Dna, supplied by BEI Resources, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza virus genomic dna/product/BEI Resources
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    influenza virus genomic dna - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    A sequence of images illustrating the plasma separation process. (A) A 200 μL of blood sample spiked with S. mansoni DNA was loaded into the superhydrophobic plasma separator. (B) When the top cover was closed, the blood was sandwiched between

    Journal: Lab on a chip

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator

    doi: 10.1039/c5lc01235j

    Figure Lengend Snippet: A sequence of images illustrating the plasma separation process. (A) A 200 μL of blood sample spiked with S. mansoni DNA was loaded into the superhydrophobic plasma separator. (B) When the top cover was closed, the blood was sandwiched between

    Article Snippet: 200 μL of the whole blood spiked with S. mansoni DNA (obtained from the Schistosomiasis Resource Center, for distribution by BEI Resources, NIAID, NIH) was loaded into the blood well ( ).

    Techniques: Sequencing

    (A) Recovery efficiency of S. mansoni genomic DNA on our plasma separator at various concentrations. (B) Endpoint, fluorescence images of intercalating dye in three amplification chambers, target DNA amount in each chamber is 10 fg, 1 fg and 0 fg (negative

    Journal: Lab on a chip

    Article Title: A High-Efficiency Superhydrophobic Plasma Separator

    doi: 10.1039/c5lc01235j

    Figure Lengend Snippet: (A) Recovery efficiency of S. mansoni genomic DNA on our plasma separator at various concentrations. (B) Endpoint, fluorescence images of intercalating dye in three amplification chambers, target DNA amount in each chamber is 10 fg, 1 fg and 0 fg (negative

    Article Snippet: 200 μL of the whole blood spiked with S. mansoni DNA (obtained from the Schistosomiasis Resource Center, for distribution by BEI Resources, NIAID, NIH) was loaded into the blood well ( ).

    Techniques: Fluorescence, Amplification

    Overview of experimental design. During phase I, DNA extraction treatments were performed on subdivided tissue, with efficiency of SSU gene amplification assessed using gel screening of PCR products. The green check mark and red X indicate that amplicons from the treatment were and were not chosen for sequencing, respectively. During phase II, well-performing PB, VG, PG, and VGl extracts were amplified and sequenced for microbial community analysis. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass

    Journal: Microbiome

    Article Title: Optimization of DNA extraction for advancing coral microbiota investigations

    doi: 10.1186/s40168-017-0229-y

    Figure Lengend Snippet: Overview of experimental design. During phase I, DNA extraction treatments were performed on subdivided tissue, with efficiency of SSU gene amplification assessed using gel screening of PCR products. The green check mark and red X indicate that amplicons from the treatment were and were not chosen for sequencing, respectively. During phase II, well-performing PB, VG, PG, and VGl extracts were amplified and sequenced for microbial community analysis. PS PowerSoil, PP PowerPlant Pro, PB PowerBiofilm, VG UC Vortex Garnet, PG UC Powerlyzer Glass, VGl UC Vortex Glass

    Article Snippet: In addition, two positive DNA controls obtained from Escherichia coli (Promega) and the Human Microbiome Project mock community DNA (BEI Resources, cat # HM-276D) and a negative control (U.V. sterilized DNA-free water) were amplified, barcoded, and included in the library pool.

    Techniques: DNA Extraction, Amplification, Polymerase Chain Reaction, Sequencing

    Detection of dual infection (both S . mansoni and S . haematobium ) by species-specific repeat DNA amplification from single urine sample. The dual infection for both species is categorized into two different age groups (Group A: 7–12 years and Group B: 13–15 years) and for both female and male participants.

    Journal: PLoS ONE

    Article Title: Detection of duo-schistosome infection from filtered urine samples from school children in Zambia after MDA

    doi: 10.1371/journal.pone.0189400

    Figure Lengend Snippet: Detection of dual infection (both S . mansoni and S . haematobium ) by species-specific repeat DNA amplification from single urine sample. The dual infection for both species is categorized into two different age groups (Group A: 7–12 years and Group B: 13–15 years) and for both female and male participants.

    Article Snippet: For every reaction, three different controls were used: 1) S . mansoni and S . haematobium genomic DNA (BEI Resources, Manassas, VA, USA) as positive control; 2) extracted DNA from urine collected in-house as negative control; and 3) nuclease-free-water as water control (Sigma-Aldrich, St. Louis, MO, USA).

    Techniques: Infection, Amplification

    Overview of mycobacterial DnaB inteins. a Relationship of DnaB inteins in three mycobacterial species. The two M. smegmatis ( Msm ) DnaB inteins (DnaBi1 and DnaBi2) were aligned to the single DnaB inteins in M. leprae ( Mle ) and M. tuberculosis ( Mtu ) using a protein pairwise alignment (EMBOSS Needle; http://www.ebi.ac.uk/Tools/psa/emboss_needle ). The amino acid percent identity between inteins (red) are shown. The number of residues for the intein and extein fragments (white) are indicated and the homing endonuclease (HEN) is shown in black. The N-terminal domain of DnaB is indicated in light blue shading and the C-terminal ATPase is indicated in gray shading. NTD N-terminal domain, CTD C-terminal domain, aa amino acid. b Localization of DnaB intein insertions. A structure model of the DnaB ATPase domain (residues 200–461) is shown 54 . The insertion sites of DnaBi1 and DnaBi2 are indicated by the S + 1 in purple. The P-loop, where DnaBi1 is inserted, is cyan and the H4 motif/DNA-binding loop, where DnaBi2 is found, is orange. c Intein splicing mechanisms. The class 3 mechanism (top) is used by Msm DnaBi1 and Mle DnaBi, whereas the canonical class 1 pathway (bottom) is used by Msm DnaBi2 and Mtu DnaBi. See the main text and Supplementary Figure 1 for detailed splicing description and steps (boxed numbers). Residue numbering refers to Msm inteins

    Journal: Nature Communications

    Article Title: Mycobacterial DnaB helicase intein as oxidative stress sensor

    doi: 10.1038/s41467-018-06554-x

    Figure Lengend Snippet: Overview of mycobacterial DnaB inteins. a Relationship of DnaB inteins in three mycobacterial species. The two M. smegmatis ( Msm ) DnaB inteins (DnaBi1 and DnaBi2) were aligned to the single DnaB inteins in M. leprae ( Mle ) and M. tuberculosis ( Mtu ) using a protein pairwise alignment (EMBOSS Needle; http://www.ebi.ac.uk/Tools/psa/emboss_needle ). The amino acid percent identity between inteins (red) are shown. The number of residues for the intein and extein fragments (white) are indicated and the homing endonuclease (HEN) is shown in black. The N-terminal domain of DnaB is indicated in light blue shading and the C-terminal ATPase is indicated in gray shading. NTD N-terminal domain, CTD C-terminal domain, aa amino acid. b Localization of DnaB intein insertions. A structure model of the DnaB ATPase domain (residues 200–461) is shown 54 . The insertion sites of DnaBi1 and DnaBi2 are indicated by the S + 1 in purple. The P-loop, where DnaBi1 is inserted, is cyan and the H4 motif/DNA-binding loop, where DnaBi2 is found, is orange. c Intein splicing mechanisms. The class 3 mechanism (top) is used by Msm DnaBi1 and Mle DnaBi, whereas the canonical class 1 pathway (bottom) is used by Msm DnaBi2 and Mtu DnaBi. See the main text and Supplementary Figure 1 for detailed splicing description and steps (boxed numbers). Residue numbering refers to Msm inteins

    Article Snippet: PCR fragments were amplified using CloneAmp HiFi PCR Premix (Clontech) from Msm mc2 155, Mtu H37Rv, or Mle Br 4923 genomic DNA (BEI Resources).

    Techniques: Binding Assay

    Experimental Workflow. Approximately 1 mL of saliva was collected from study participants using a commercially available kit from OMNIgene. Bacteria within the saliva samples were then vigorously lysed using both enzymatic and mechanical techniques. After purifying genomic DNA, amplicons from the 16S rRNA gene were generated, sequenced on an Illumina MiSeq, and analyzed using the DADA2 computational pipeline to determine microbiome composition.

    Journal: Scientific Reports

    Article Title: The salivary microbiome is consistent between subjects and resistant to impacts of short-term hospitalization

    doi: 10.1038/s41598-017-11427-2

    Figure Lengend Snippet: Experimental Workflow. Approximately 1 mL of saliva was collected from study participants using a commercially available kit from OMNIgene. Bacteria within the saliva samples were then vigorously lysed using both enzymatic and mechanical techniques. After purifying genomic DNA, amplicons from the 16S rRNA gene were generated, sequenced on an Illumina MiSeq, and analyzed using the DADA2 computational pipeline to determine microbiome composition.

    Article Snippet: To do so, we compared the performance of two commonly utilized regions, V1V2 and V4V5, using a mock community composed of bacterial genomic DNA, developed for the Human Microbiome Project (HMP) and available through BEI Resources (HM-783D).

    Techniques: Generated

    Hierarchical clustering of mixed samples demonstrates the resolution capabilities of the UBDA array . This dendogram and heat map illustrates a unique bio-signature pattern obtained from Lactobacillus plantarum , mixed sample (synthetic mixture in a 4:1 ratio of L. plantarum and Streptococcus mitis ), S. mitis , mixed sample (a synthetic mixture of L. plantarum and S. mitis genomic DNA in a ratio of 4:1 with a spike-in of pBluescript plasmid at 50 ng) and pBluescript plasmid. Normalized data from the 9-mer data set were filtered for intensity signals greater than the 20 th percentile. Only intensity signals with a fold change of 5 or greater were included. These 36,059 elements were subjected to hierarchical clustering with Euclidean distance being used as a similarity measure. The signal intensity values were represented on a log 2 scale and range from 8.4 to 13.4.

    Journal: BMC Microbiology

    Article Title: A species independent universal bio-detection microarray for pathogen forensics and phylogenetic classification of unknown microorganisms

    doi: 10.1186/1471-2180-11-132

    Figure Lengend Snippet: Hierarchical clustering of mixed samples demonstrates the resolution capabilities of the UBDA array . This dendogram and heat map illustrates a unique bio-signature pattern obtained from Lactobacillus plantarum , mixed sample (synthetic mixture in a 4:1 ratio of L. plantarum and Streptococcus mitis ), S. mitis , mixed sample (a synthetic mixture of L. plantarum and S. mitis genomic DNA in a ratio of 4:1 with a spike-in of pBluescript plasmid at 50 ng) and pBluescript plasmid. Normalized data from the 9-mer data set were filtered for intensity signals greater than the 20 th percentile. Only intensity signals with a fold change of 5 or greater were included. These 36,059 elements were subjected to hierarchical clustering with Euclidean distance being used as a similarity measure. The signal intensity values were represented on a log 2 scale and range from 8.4 to 13.4.

    Article Snippet: Brucella species, Cryptosporidium parvum, Lactobacillus plantarum , Streptococcus mitis , Escherichia coli and Influenza virus genomic DNA was obtained from BEI resources and ATCC (Manasses, VA).

    Techniques: Plasmid Preparation