dna 1000 chip Search Results


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  • 94
    Agilent technologies dna 1000 chip
    Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using <t>DNA</t> 1000 chip (Agilent Technologies). For each sample three biological replicates were performed
    Dna 1000 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 2895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 2895 article reviews
    Price from $9.99 to $1999.99
    dna 1000 chip - by Bioz Stars, 2020-08
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    91
    Agilent technologies dna 1000 chip kit
    Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using <t>DNA</t> 1000 chip (Agilent Technologies). For each sample three biological replicates were performed
    Dna 1000 Chip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 623 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 chip kit/product/Agilent technologies
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    Price from $9.99 to $1999.99
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    88
    Agilent technologies bioanalyser dna 1000 chip
    Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using <t>DNA</t> 1000 chip (Agilent Technologies). For each sample three biological replicates were performed
    Bioanalyser Dna 1000 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 88/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyser dna 1000 chip/product/Agilent technologies
    Average 88 stars, based on 101 article reviews
    Price from $9.99 to $1999.99
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    89
    Agilent technologies dna 1000 chips
    gRNA mediated cleavage reveals mir-29b-1 as the main source of mature miR-29b. ( a ) Surveyor assay detecting the mutations on miR-29b gene locus. mmu-mir-29b-1 was shown to have mutations in cas1-1, cas1-2, cas2-1 and cas2-2; mmu-mir-29b-2 displayed mutations in cas2-1, cas2-2, cas3-1, cas3-2 and cas3-3. ( b ) Surveyor assay showed mutations on hsa-mir-29b-1 and hsa-mir-29b-2 sequences in clone cas1-1, cas1-2, cas1-3 and cas1-4. Surveyor assay products were visualized on Bioanalyser equipment using <t>DNA</t> 1000 chip. Full-length Bioanalyser images for ( a , b ) are presented in Supplementary Fig. 5 .
    Dna 1000 Chips, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 488 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 chips/product/Agilent technologies
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    94
    Agilent technologies bioanalyzer dna 1000 chip
    CRISPR/Cas9 deletion of MTA1 in breast cancer cells. a . T7 endonuclease assay PCR results. Electropherogram of the T7 endonuclease digestion of MTA1 genomic PCR products visualized on an Agilent <t>Bioanalyzer</t> <t>DNA</t> 1000 Chip. Due to location of sgRNA target site, digestion of PCR products was predicted to generate the following fragments: wildtype 800 bp, sgRNA #1: 500 and 310 bp, sgRNA#3: 700 and 100 bp, and sgRNA #5: 760 and 40 bp. b . Western blot of MTA1 in MCF7 and MDA-MB-231 MTA1 knockout cells. GAPDH is included as an equal loading control. c . Cell proliferation assay of MCF7 and MDA-MB-231 knockout cells, compared to cells expressing an empty vector, n = 4 **** p
    Bioanalyzer Dna 1000 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 2376 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer dna 1000 chip/product/Agilent technologies
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    89
    Agilent technologies dna 1000 nano chip kit
    CRISPR/Cas9 deletion of MTA1 in breast cancer cells. a . T7 endonuclease assay PCR results. Electropherogram of the T7 endonuclease digestion of MTA1 genomic PCR products visualized on an Agilent <t>Bioanalyzer</t> <t>DNA</t> 1000 Chip. Due to location of sgRNA target site, digestion of PCR products was predicted to generate the following fragments: wildtype 800 bp, sgRNA #1: 500 and 310 bp, sgRNA#3: 700 and 100 bp, and sgRNA #5: 760 and 40 bp. b . Western blot of MTA1 in MCF7 and MDA-MB-231 MTA1 knockout cells. GAPDH is included as an equal loading control. c . Cell proliferation assay of MCF7 and MDA-MB-231 knockout cells, compared to cells expressing an empty vector, n = 4 **** p
    Dna 1000 Nano Chip Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 89/100, based on 257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 nano chip kit/product/Agilent technologies
    Average 89 stars, based on 257 article reviews
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    91
    Agilent technologies dna 1000 microfluidic chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    Dna 1000 Microfluidic Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 microfluidic chip/product/Agilent technologies
    Average 91 stars, based on 4 article reviews
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    90
    Agilent technologies bio analyzer dna 1000 chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    Bio Analyzer Dna 1000 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bio analyzer dna 1000 chip/product/Agilent technologies
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bio analyzer dna 1000 chip - by Bioz Stars, 2020-08
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    91
    Illumina Inc bioanalyzer dna 1000 chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    Bioanalyzer Dna 1000 Chip, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bioanalyzer dna 1000 chip/product/Illumina Inc
    Average 91 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    bioanalyzer dna 1000 chip - by Bioz Stars, 2020-08
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    90
    Agilent technologies 2100 bioanalyzer dna 1000 chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    2100 Bioanalyzer Dna 1000 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2100 bioanalyzer dna 1000 chip/product/Agilent technologies
    Average 90 stars, based on 577 article reviews
    Price from $9.99 to $1999.99
    2100 bioanalyzer dna 1000 chip - by Bioz Stars, 2020-08
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    92
    Agilent technologies dna 1000 bioanalyser 2100 chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    Dna 1000 Bioanalyser 2100 Chip, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 bioanalyser 2100 chip/product/Agilent technologies
    Average 92 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    dna 1000 bioanalyser 2100 chip - by Bioz Stars, 2020-08
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    90
    Bio-Rad dna 1000 chip
    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a <t>microfluidic</t> <t>DNA</t> 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).
    Dna 1000 Chip, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna 1000 chip/product/Bio-Rad
    Average 90 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
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    Image Search Results


    Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using DNA 1000 chip (Agilent Technologies). For each sample three biological replicates were performed

    Journal: SpringerPlus

    Article Title: High quality RNA extraction from Maqui berry for its application in next-generation sequencing

    doi: 10.1186/s40064-016-2906-x

    Figure Lengend Snippet: Electrophoretogram of sequencing libraries. The graph shows the length distribution curves of sequencing libraries obtained with Illumina TruSeqTM RNA sample preparation kit (Low-Throughput protocol) according to manufacturer’s protocol. The length of DNA fragments was between 200 and 700 pb. Curves were generated on a 2100 bioanalyzer using DNA 1000 chip (Agilent Technologies). For each sample three biological replicates were performed

    Article Snippet: Subsequently, the libraries were validated using DNA 1000 chip (on Agilent Technologies 2100 bioanalyzer).

    Techniques: Sequencing, Sample Prep, Generated, Chromatin Immunoprecipitation

    gRNA mediated cleavage reveals mir-29b-1 as the main source of mature miR-29b. ( a ) Surveyor assay detecting the mutations on miR-29b gene locus. mmu-mir-29b-1 was shown to have mutations in cas1-1, cas1-2, cas2-1 and cas2-2; mmu-mir-29b-2 displayed mutations in cas2-1, cas2-2, cas3-1, cas3-2 and cas3-3. ( b ) Surveyor assay showed mutations on hsa-mir-29b-1 and hsa-mir-29b-2 sequences in clone cas1-1, cas1-2, cas1-3 and cas1-4. Surveyor assay products were visualized on Bioanalyser equipment using DNA 1000 chip. Full-length Bioanalyser images for ( a , b ) are presented in Supplementary Fig. 5 .

    Journal: Scientific Reports

    Article Title: Novel miR-29b target regulation patterns are revealed in two different cell lines

    doi: 10.1038/s41598-019-53868-x

    Figure Lengend Snippet: gRNA mediated cleavage reveals mir-29b-1 as the main source of mature miR-29b. ( a ) Surveyor assay detecting the mutations on miR-29b gene locus. mmu-mir-29b-1 was shown to have mutations in cas1-1, cas1-2, cas2-1 and cas2-2; mmu-mir-29b-2 displayed mutations in cas2-1, cas2-2, cas3-1, cas3-2 and cas3-3. ( b ) Surveyor assay showed mutations on hsa-mir-29b-1 and hsa-mir-29b-2 sequences in clone cas1-1, cas1-2, cas1-3 and cas1-4. Surveyor assay products were visualized on Bioanalyser equipment using DNA 1000 chip. Full-length Bioanalyser images for ( a , b ) are presented in Supplementary Fig. 5 .

    Article Snippet: Surveyor nuclease digestion products were visualized on Bioanalyser equipment using DNA 1000 chips (Agilent Technologies) containing interconnected microchannels for separation of nucleic acid fragment based on their sizes.

    Techniques: Chromatin Immunoprecipitation

    CRISPR/Cas9 deletion of MTA1 in breast cancer cells. a . T7 endonuclease assay PCR results. Electropherogram of the T7 endonuclease digestion of MTA1 genomic PCR products visualized on an Agilent Bioanalyzer DNA 1000 Chip. Due to location of sgRNA target site, digestion of PCR products was predicted to generate the following fragments: wildtype 800 bp, sgRNA #1: 500 and 310 bp, sgRNA#3: 700 and 100 bp, and sgRNA #5: 760 and 40 bp. b . Western blot of MTA1 in MCF7 and MDA-MB-231 MTA1 knockout cells. GAPDH is included as an equal loading control. c . Cell proliferation assay of MCF7 and MDA-MB-231 knockout cells, compared to cells expressing an empty vector, n = 4 **** p

    Journal: Cell Communication and Signaling : CCS

    Article Title: Metastasis-associated protein 1 (MTA1) is transferred by exosomes and contributes to the regulation of hypoxia and estrogen signaling in breast cancer cells

    doi: 10.1186/s12964-019-0325-7

    Figure Lengend Snippet: CRISPR/Cas9 deletion of MTA1 in breast cancer cells. a . T7 endonuclease assay PCR results. Electropherogram of the T7 endonuclease digestion of MTA1 genomic PCR products visualized on an Agilent Bioanalyzer DNA 1000 Chip. Due to location of sgRNA target site, digestion of PCR products was predicted to generate the following fragments: wildtype 800 bp, sgRNA #1: 500 and 310 bp, sgRNA#3: 700 and 100 bp, and sgRNA #5: 760 and 40 bp. b . Western blot of MTA1 in MCF7 and MDA-MB-231 MTA1 knockout cells. GAPDH is included as an equal loading control. c . Cell proliferation assay of MCF7 and MDA-MB-231 knockout cells, compared to cells expressing an empty vector, n = 4 **** p

    Article Snippet: The T7EI digestion products were visualized by running on an Agilent Bioanalyzer DNA 1000 Chip (Agilent Technologies).

    Techniques: CRISPR, Polymerase Chain Reaction, Chromatin Immunoprecipitation, Western Blot, Multiple Displacement Amplification, Knock-Out, Proliferation Assay, Expressing, Plasmid Preparation

    Validation of novel miRNAs by qRT-PCR and northern blots. Panel A shows amplification products of qRT-PCR in three RNA Pools (P1-P3) on Bioanalyzer DNA 1000 Chip, Panel B on conventional 3% agarose gels. Negative controls included a no template control for reverse transcription (NTRT), a RT reaction without enzyme (RT-) and a no template PCR control for each specific primer (NTC). As the used qRT-PCR system depends on poly-adenylation at the 3′ end of mature miRNAs followed by reverse transcription using an oligo-dT primer that includes a universal tag sequence for the qPCR, amplification products of mature miRNAs are ≈80–95 bps depending on the number of A′s added to the miRNA sequence. The ladder bands shown represent 50 and 100 bps. For 11 miRNAs specific bands at 80–90 bps could be detected. All PCR products were subcloned into pGEM and Sanger sequenced (see Supplementary Table S4) to verify specific amplification of novel miRNAs. Panels C and D show northern blots detecting mature miRs-1005–5p (C) and -3p (D) with sequence specific radio-labelled probes (left side) in HEK293T cells transfected with pSG5 vector with inserted mir-1005 precursor sequence. The right size of the novel mature miRNAs was confirmed by the stripping and rehybridization of both nylon membranes with specific radio-labelled probes of the high confident miR-20a-5p (right side). Loading control demonstrates equal RNA amounts in all lanes.

    Journal: Nucleic Acids Research

    Article Title: Prioritizing and selecting likely novel miRNAs from NGS data

    doi: 10.1093/nar/gkv1335

    Figure Lengend Snippet: Validation of novel miRNAs by qRT-PCR and northern blots. Panel A shows amplification products of qRT-PCR in three RNA Pools (P1-P3) on Bioanalyzer DNA 1000 Chip, Panel B on conventional 3% agarose gels. Negative controls included a no template control for reverse transcription (NTRT), a RT reaction without enzyme (RT-) and a no template PCR control for each specific primer (NTC). As the used qRT-PCR system depends on poly-adenylation at the 3′ end of mature miRNAs followed by reverse transcription using an oligo-dT primer that includes a universal tag sequence for the qPCR, amplification products of mature miRNAs are ≈80–95 bps depending on the number of A′s added to the miRNA sequence. The ladder bands shown represent 50 and 100 bps. For 11 miRNAs specific bands at 80–90 bps could be detected. All PCR products were subcloned into pGEM and Sanger sequenced (see Supplementary Table S4) to verify specific amplification of novel miRNAs. Panels C and D show northern blots detecting mature miRs-1005–5p (C) and -3p (D) with sequence specific radio-labelled probes (left side) in HEK293T cells transfected with pSG5 vector with inserted mir-1005 precursor sequence. The right size of the novel mature miRNAs was confirmed by the stripping and rehybridization of both nylon membranes with specific radio-labelled probes of the high confident miR-20a-5p (right side). Loading control demonstrates equal RNA amounts in all lanes.

    Article Snippet: Specific amplification of novel miRNAs was satisfactorily demonstrated by a qRT-PCR product with (i) a melting temperature of 75°C ± 1.5C°; (ii) a mean raw Ct value of the product in the three pools of < 35 and (iii) an assay dependent product length of 80–90bp as evidenced on an DNA 1000 Bioanalyzer chip (Agilent Technologies) and conventional 3% agarose gels.

    Techniques: Quantitative RT-PCR, Northern Blot, Amplification, Chromatin Immunoprecipitation, Polymerase Chain Reaction, Sequencing, Real-time Polymerase Chain Reaction, Transfection, Plasmid Preparation, Stripping Membranes

    PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a microfluidic DNA 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).

    Journal: PLoS ONE

    Article Title: Cross-Contamination of a UROtsa Stock with T24 Cells - Molecular Comparison of Different Cell Lines and Stocks

    doi: 10.1371/journal.pone.0064139

    Figure Lengend Snippet: PCR analysis of cell lines and UROtsa stocks to detect large T-antigen. (A) PCR reactions were resolved on a 2% agarose gel. SV40gp6 sequences (5′-, 3′-, and middle part) are absent in UROtsa-1 (lanes 2, 10, 18), UROtsa-3/T24 (lanes 3, 11, 19), UROtsa-4 (lanes 4, 12, 20), HeLa (negative control, lanes 5, 13, 21) and T24 (negative control, lanes 6, 14, 22) but not in BEAS-2B (positive control, lanes 7, 15, 23). No-template controls are in lanes 1, 9, and 17. A 100 bp ladder served as size marker (lanes 8, 16, 24). (B) PCR reactions of reverse-transcribed mRNA were resolved on a microfluidic DNA 1000 chip (Bioanalyzer). Large T-antigen expression (expected product size: 65 bp in lanes 2–7 [24] and 304 bp in lanes 8–13 [23] ) is absent in UROtsa-3/T24 (lanes 3 and 8), UROtsa-4 (lanes 4 and 9), HeLa (negative control, lanes 5 and 10), and T24 (negative control, lanes 6 and 11) but not in BEAS-2B (positive control, lanes 7 and 12). A no-template control was loaded in lanes 2 and 13. Size markers (lane 1) are 15 (green), 25, 50, 100, 150, 200, 300, 400, 500, 700, 850, 1000, and 1500 bp (purple).

    Article Snippet: PCR products were visualized on a Bioanalyzer with a microfluidic DNA 1000 chip (Agilent).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control, Positive Control, Marker, Chromatin Immunoprecipitation, Expressing