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  • 86
    Millipore dmpp
    Dmpp, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmpp/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmpp - by Bioz Stars, 2023-01
    86/100 stars
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    86
    EuroChem Agro GmbH dmpp
    Dmpp, supplied by EuroChem Agro GmbH, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmpp/product/EuroChem Agro GmbH
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dmpp - by Bioz Stars, 2023-01
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    86
    ICN Biomedicals dmpp
    Dmpp, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    dmpp - by Bioz Stars, 2023-01
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    86
    Thermo Fisher dmpp
    Dmpp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmpp/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    dmpp - by Bioz Stars, 2023-01
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    86
    Alomone Labs dmpp
    GL-dSACs exhibit nicotinic responses to endogenous ACh release. A, GL-dSAC activity was recorded during focal application of broad-spectrum nicotinic agonist <t>DMPP.</t> B, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. C, D, Focal application of DMPP consistently increased the spontaneous firing rate of the cell shown in B. Results are plotted as in Figure 6. Insets, Magnification of 1-s-long periods of firing before and during DMPP appplication. E, Voltage-clamp recording of the mean inward current evoked by DMPP in the cell shown in B. Bath application of Mec abolished the inward current. F, DMPP application significantly increased GL-dSAC spontaneous firing rates (9.2 ± 3.9 vs 22.0 ± 7.5 Hz, before vs during DMPP application; n = 5; p = 0.013, two-tailed paired t test). Red points denote cell shown in B–E. G, Mec blocked the DMPP-evoked inward current in GL-dSACs, leading to a significant reduction in current amplitude (325.5 ± 75.6 vs 12.0 ± 6.1 pA, before vs after Mec application; n = 5; p = 7.3 × 10−4, two-tailed paired t test). Red points denote cell shown in B–E. H, Synaptic input to GL-dSACs was recorded while stimulating the IPL and blocking glutamatergic and GABAergic receptors. I, J, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. K, IPL stimulation (10 μA) in the presence <t>of</t> <t>NBQX/AP5/GBZ</t> evoked a robust EPSC in the cell shown in I and J that was abolished rapidly by Mec application. Inset, Mean synaptic response evoked by IPL stimulation (arrowhead) before and after Mec application (scale bar, 100 ms/50 pA). Individual trials are shown in lighter colors. L, Mean postsynaptic nicotinic current recorded in GL-dSACs (peak: 57.7 ± 38.8 pA; decay constant: 29.2 ± 7.2 ms; n = 6) after IPL stimulation (arrowhead; 10–15 μA) calculated by subtracting the mean current after Mec application from the mean current before Mec application (in the presence of NBQX/AP5/GBZ).
    Dmpp, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dmpp/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
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    dmpp - by Bioz Stars, 2023-01
    86/100 stars
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    Image Search Results


    GL-dSACs exhibit nicotinic responses to endogenous ACh release. A, GL-dSAC activity was recorded during focal application of broad-spectrum nicotinic agonist DMPP. B, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. C, D, Focal application of DMPP consistently increased the spontaneous firing rate of the cell shown in B. Results are plotted as in Figure 6. Insets, Magnification of 1-s-long periods of firing before and during DMPP appplication. E, Voltage-clamp recording of the mean inward current evoked by DMPP in the cell shown in B. Bath application of Mec abolished the inward current. F, DMPP application significantly increased GL-dSAC spontaneous firing rates (9.2 ± 3.9 vs 22.0 ± 7.5 Hz, before vs during DMPP application; n = 5; p = 0.013, two-tailed paired t test). Red points denote cell shown in B–E. G, Mec blocked the DMPP-evoked inward current in GL-dSACs, leading to a significant reduction in current amplitude (325.5 ± 75.6 vs 12.0 ± 6.1 pA, before vs after Mec application; n = 5; p = 7.3 × 10−4, two-tailed paired t test). Red points denote cell shown in B–E. H, Synaptic input to GL-dSACs was recorded while stimulating the IPL and blocking glutamatergic and GABAergic receptors. I, J, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. K, IPL stimulation (10 μA) in the presence of NBQX/AP5/GBZ evoked a robust EPSC in the cell shown in I and J that was abolished rapidly by Mec application. Inset, Mean synaptic response evoked by IPL stimulation (arrowhead) before and after Mec application (scale bar, 100 ms/50 pA). Individual trials are shown in lighter colors. L, Mean postsynaptic nicotinic current recorded in GL-dSACs (peak: 57.7 ± 38.8 pA; decay constant: 29.2 ± 7.2 ms; n = 6) after IPL stimulation (arrowhead; 10–15 μA) calculated by subtracting the mean current after Mec application from the mean current before Mec application (in the presence of NBQX/AP5/GBZ).

    Journal: The Journal of Neuroscience

    Article Title: Olfactory Bulb Deep Short-Axon Cells Mediate Widespread Inhibition of Tufted Cell Apical Dendrites

    doi: 10.1523/JNEUROSCI.2880-16.2016

    Figure Lengend Snippet: GL-dSACs exhibit nicotinic responses to endogenous ACh release. A, GL-dSAC activity was recorded during focal application of broad-spectrum nicotinic agonist DMPP. B, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. C, D, Focal application of DMPP consistently increased the spontaneous firing rate of the cell shown in B. Results are plotted as in Figure 6. Insets, Magnification of 1-s-long periods of firing before and during DMPP appplication. E, Voltage-clamp recording of the mean inward current evoked by DMPP in the cell shown in B. Bath application of Mec abolished the inward current. F, DMPP application significantly increased GL-dSAC spontaneous firing rates (9.2 ± 3.9 vs 22.0 ± 7.5 Hz, before vs during DMPP application; n = 5; p = 0.013, two-tailed paired t test). Red points denote cell shown in B–E. G, Mec blocked the DMPP-evoked inward current in GL-dSACs, leading to a significant reduction in current amplitude (325.5 ± 75.6 vs 12.0 ± 6.1 pA, before vs after Mec application; n = 5; p = 7.3 × 10−4, two-tailed paired t test). Red points denote cell shown in B–E. H, Synaptic input to GL-dSACs was recorded while stimulating the IPL and blocking glutamatergic and GABAergic receptors. I, J, Whole-cell recording of a representative EYFP-expressing GL-dSAC. Scale bar, 20 μm. K, IPL stimulation (10 μA) in the presence of NBQX/AP5/GBZ evoked a robust EPSC in the cell shown in I and J that was abolished rapidly by Mec application. Inset, Mean synaptic response evoked by IPL stimulation (arrowhead) before and after Mec application (scale bar, 100 ms/50 pA). Individual trials are shown in lighter colors. L, Mean postsynaptic nicotinic current recorded in GL-dSACs (peak: 57.7 ± 38.8 pA; decay constant: 29.2 ± 7.2 ms; n = 6) after IPL stimulation (arrowhead; 10–15 μA) calculated by subtracting the mean current after Mec application from the mean current before Mec application (in the presence of NBQX/AP5/GBZ).

    Article Snippet: NBQX (Tocris Bioscience and Sigma-Aldrich), AP5 (Tocris Bioscience), GBZ (Tocris Bioscience), DMPP (Alomone Labs), and Mec (Tocris Bioscience and Alomone Labs) were added to the bath or focally applied as indicated.

    Techniques: Activity Assay, Expressing, Two Tailed Test, Blocking Assay