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Image Search Results
Journal: Cell Stem Cell
Article Title: Macrophage-Derived Extracellular Succinate Licenses Neural Stem Cells to Suppress Chronic Neuroinflammation
doi: 10.1016/j.stem.2018.01.020
Figure Lengend Snippet:
Article Snippet: Tissues were isolated, kept in ice-cold complete IMDM medium [5% FBS, 1% GlutaMAX (GIBCO), 1% pen/strep (Invitrogen) in IMDM (GIBCO)], sectioned into small pieces (≈5-10 mm 3 ) and incubated at 37°C with 1 mL of digestion buffer [2 mg/ml collagenase (Sigma), 0.2 mg/ml
Techniques: Plasmid Preparation, Purification, Recombinant, Flow Cytometry, Staining, Activation Assay, LDH Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Colorimetric Assay, Expressing, Microarray, Software
Journal: Science translational medicine
Article Title: Human limbal biopsy–derived stromal stem cells prevent corneal scarring
doi: 10.1126/scitranslmed.3009644
Figure Lengend Snippet: (A) Phase-contrast images of primary cells cultured from limbal biopsy tissue prepared by digestion with collagenase only. In initial plating (P0), red dashed lines mark islands of epithelial cells. Scale bars, 50 μm. (B) The length of time (in days) required to expand cells from P0 to P3 was compared for LBSCs prepared with dispase and collagenase (DSP + COL) or collagenase only (COL), expanding cells in either HS or FBS. Data are means ± SD (n = 4). P value determined by two-way analysis of variance (ANOVA). (C) Clonal growth of LBSCs in HS and FBS. (D) Percentage of clonogenic cells in P3 cultures. Data are means ± SD (n = 4). (E) Colony size was calculated with Fiji image analysis software. Data are means ± SD (n > 400). P values in (D) and (E) were determined by a two-sided t test. (F) Corneal fibroblasts in FBS did not exhibit clonal growth (n = 4).
Article Snippet: The remaining four segments were first incubated for 40 min in
Techniques: Cell Culture, Software