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  • 94
    Thermo Fisher dionex dnapac pa 100
    Dionex Dnapac Pa 100, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher dionex dnapac pa
    HPLC trace of before and after 90°C, 5 min treatment. The oligonucleotides containing (A) riboFTg(5 R ), (B) riboFTg(5 S ) or (C) araFTg(5 S ) were analyzed by HPLC using a Dionex <t>DNAPac</t> <t>PA-100</t> ion exchange column. The analysis was achieved with buffer
    Dionex Dnapac Pa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher dionex dnapac pa 100 column
    Synthesis of RNA-peptide conjugates based on the modified solid supports 9 and 10 . Anion-exchange HPLC profiles of crude and purified (insets) conjugates S7 , S4 , and S6 ( A – C , upper panels) and LC-ESI mass spectra of purified products ( A – C , lower panels). Anion-exchange chromatography conditions: <t>Dionex</t> <t>DNAPac®PA-100</t> (4 × 250 mm) column; temperature: 60 °C; flow rate: 1 mL/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–35% B in A within 30 min; UV detection at 260 nm.
    Dionex Dnapac Pa 100 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher semipreparative dionex dnapac pa 100 column
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Semipreparative Dionex Dnapac Pa 100 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher preparative dionex dnapac pa 200 column
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Preparative Dionex Dnapac Pa 200 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher biolc dnapac pa
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Biolc Dnapac Pa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher dnapac pa 200 column
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Dnapac Pa 200 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dnapac pa 100 9×250 column
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Dnapac Pa 100 9×250 Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher anion exchange chromatography
    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: <t>Dionex</t> <t>DNAPac</t> <t>PA‐100</t> (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.
    Anion Exchange Chromatography, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    HPLC trace of before and after 90°C, 5 min treatment. The oligonucleotides containing (A) riboFTg(5 R ), (B) riboFTg(5 S ) or (C) araFTg(5 S ) were analyzed by HPLC using a Dionex DNAPac PA-100 ion exchange column. The analysis was achieved with buffer

    Journal: Chembiochem : a European journal of chemical biology

    Article Title: NEIL1 Binding to DNA containing 2?-Fluorothymidine Glycol Stereoisomers and the Effect of Editing

    doi: 10.1002/cbic.201200139

    Figure Lengend Snippet: HPLC trace of before and after 90°C, 5 min treatment. The oligonucleotides containing (A) riboFTg(5 R ), (B) riboFTg(5 S ) or (C) araFTg(5 S ) were analyzed by HPLC using a Dionex DNAPac PA-100 ion exchange column. The analysis was achieved with buffer

    Article Snippet: The residues were dissolved in 0.1 M triethylammonium acetate (pH 7.0), and the oligonucleotides were analyzed and purified by HPLC using a Dionex DNAPac PA-100 ion exchange column.

    Techniques: High Performance Liquid Chromatography

    Synthesis of RNA-peptide conjugates based on the modified solid supports 9 and 10 . Anion-exchange HPLC profiles of crude and purified (insets) conjugates S7 , S4 , and S6 ( A – C , upper panels) and LC-ESI mass spectra of purified products ( A – C , lower panels). Anion-exchange chromatography conditions: Dionex DNAPac®PA-100 (4 × 250 mm) column; temperature: 60 °C; flow rate: 1 mL/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–35% B in A within 30 min; UV detection at 260 nm.

    Journal: Bioorganic & Medicinal Chemistry

    Article Title: Functionalized polystyrene supports for solid-phase synthesis of glycyl-, alanyl-, and isoleucyl-RNA conjugates as hydrolysis-resistant mimics of peptidyl-tRNAs

    doi: 10.1016/j.bmc.2011.07.018

    Figure Lengend Snippet: Synthesis of RNA-peptide conjugates based on the modified solid supports 9 and 10 . Anion-exchange HPLC profiles of crude and purified (insets) conjugates S7 , S4 , and S6 ( A – C , upper panels) and LC-ESI mass spectra of purified products ( A – C , lower panels). Anion-exchange chromatography conditions: Dionex DNAPac®PA-100 (4 × 250 mm) column; temperature: 60 °C; flow rate: 1 mL/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–35% B in A within 30 min; UV detection at 260 nm.

    Article Snippet: 4.2.4 Analysis and purification of RNA-peptide conjugates Analysis of crude products after deprotection was performed by anion-exchange HPLC on a Dionex DNAPac®PA-100 column (4x250 mm) at 60 °C.

    Techniques: Modification, High Performance Liquid Chromatography, Purification, Chromatography, Flow Cytometry

    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: Dionex DNAPac PA‐100 (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Journal: EMBO Reports

    Article Title: Molecular insights into protein synthesis with proline residues

    doi: 10.15252/embr.201642943

    Figure Lengend Snippet: Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: Dionex DNAPac PA‐100 (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Article Snippet: Analysis of the crude products was performed by anion‐exchange chromatography on a Dionex DNAPac PA‐100 column (4 × 250 mm) at 60°C.

    Techniques: High Performance Liquid Chromatography, Purification, Chromatography, Flow Cytometry

    Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: Dionex DNAPac PA‐100 (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Journal: EMBO Reports

    Article Title: Molecular insights into protein synthesis with proline residues

    doi: 10.15252/embr.201642943

    Figure Lengend Snippet: Chemical synthesis of hydrolysis‐resistant ACCA‐Pro and ACCA‐Pro‐Pro conjugates The strategy is exemplified by the synthetic scheme for the ACCA‐Pro‐Pro conjugate. Synthetic scheme overview. Letters indicate conditions: a) 1.25 equivalent of Fmoc‐Pro, 1.25 equivalent of O ‐(benzotriazol‐1‐yl)‐ N , N , N ', N '‐tetramethyluronium hexafluorophosphate, 1.25 equivalent of 1‐hydroxybenzotriazole hydrate, 1.5 equivalent of N , N ‐diisopropylethylamine in DMF, room temperature, 12 h, 76% for compound 2; b) 3 equivalent of adipic acid bis(pentafluorophenyl)ester, 1 equivalent of 4‐( N , N ‐dimethyl‐amino)pyridine in N , N ‐dimethylformamide/pyridine (1/1, v/v), room temperature, 1 h, 54% for compound 3; c) ˜1 equivalent of amino‐functionalized polystyrene support (GE Healthcare, Custom Primer SupportTM 200 Amino), pyridine, N , N ‐dimethylformamide, room temperature, 1 day, loading: 45 μmol/g for solid support 4. DMT, 4,4′‐dimethoxytrityl; Fmoc, N ‐(9‐fluorenyl)methoxycarbonyl; SPS, solid‐phase synthesis; AE HPLC, anion‐exchange high‐pressure liquid chromatography. Anion‐exchange HPLC profiles of crude (top) and purified (middle) ACCA‐Pro‐Pro conjugate and LC‐ESI mass spectra of purified products (bottom). Anion‐exchange chromatography conditions: Dionex DNAPac PA‐100 (4 × 250 mm) column; temperature: 60°C; flow rate: 1 ml/min; eluant A: 25 mM Tris–HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris–HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Article Snippet: The crude conjugate was purified on a semipreparative Dionex DNAPac PA‐100 column (9 × 250 mm) at 60°C with flow rate of 2 ml/min (for eluents, see above).

    Techniques: High Performance Liquid Chromatography, Purification, Chromatography, Flow Cytometry

    Synthesis of RNA-peptide conjugates based on the modified solid supports 8 and 10 . Anion-exchange HPLC profiles of crude and purified (insets) conjugates S1 and S3 ( A , B ; upper panels) and LC-ESI mass spectra of purified products ( A , B ; lower panels). Anion-exchange chromatography conditions: Dionex DNAPac®PA-100 (4 × 250 mm) column; temperature: 60 °C; flow rate: 1 mL/min; eluant A: 25 mM Tris·HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris·HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Journal: Bioorganic & Medicinal Chemistry

    Article Title: Synthesis of aminoacylated N6,N6-dimethyladenosine solid support for efficient access to hydrolysis-resistant 3′-charged tRNA mimics

    doi: 10.1016/j.bmc.2014.09.054

    Figure Lengend Snippet: Synthesis of RNA-peptide conjugates based on the modified solid supports 8 and 10 . Anion-exchange HPLC profiles of crude and purified (insets) conjugates S1 and S3 ( A , B ; upper panels) and LC-ESI mass spectra of purified products ( A , B ; lower panels). Anion-exchange chromatography conditions: Dionex DNAPac®PA-100 (4 × 250 mm) column; temperature: 60 °C; flow rate: 1 mL/min; eluant A: 25 mM Tris·HCl (pH 8.0), 6 M urea; eluant B: 25 mM Tris·HCl (pH 8.0), 6 M urea, 500 mM NaClO 4 ; gradient: 0–40% B in A within 25 min; UV detection at 260 nm.

    Article Snippet: Crude products were purified on a semi-preparative Dionex DNAPac®PA-100 column (9x250 mm) at 60 °C.

    Techniques: Modification, High Performance Liquid Chromatography, Purification, Chromatography, Flow Cytometry