Article Title: Cytotoxic, tubulin-interfering and proapoptotic activities of 4′-methylthio-trans-stilbene derivatives, analogues of trans-resveratrol
Figure Lengend Snippet: Effect of resveratrol, 3,4,5-MTS and 2,4,5-MTS on phosphatidylserine externalization and propidium iodide staining in MCF12A ( a ), MCF7 ( b ), HaCaT ( c ) and A431 ( d ) cell lines after 24 h of incubation subjected to Annexin-V binding and propidium iodide labeling followed by flow cytometry analysis. The cell populations of AV−/PI−, AV+/PI+ and AV+/PI− representing viable, dead and apoptotic cells, respectively, were estimated. Camptothecin at final concentration of 50 nM was used as a positive control. Results of three independent experiments are presented as mean ± SEM. * p
Article Snippet: Chemicals Resveratrol (purity 99%), antibiotic solution (10,000 units’ penicillin, 10 mg streptomycin and 25 μg amphotericin B per mL), camptothecin, dimethyl sulfoxide (DMSO), fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Ham’s F12 mixture, propidium iodide (PI), ribonuclease A (RNase A) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and all other compounds were provided by Sigma-Aldrich Co. (St Louis, MO, USA).
Techniques: Staining, Incubation, Binding Assay, Labeling, Flow Cytometry, Cytometry, Concentration Assay, Positive Control